LOCUS       NM_033523               2872 bp    mRNA    linear   ROD 23-JUL-2024
DEFINITION  Mus musculus sprouty-related EVH1 domain containing 2 (Spred2),
            mRNA.
ACCESSION   NM_033523
VERSION     NM_033523.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Wang,T., Gao,T., Fujisawa,M., Ohara,T., Sakaguchi,M., Yoshimura,T.
            and Matsukawa,A.
  TITLE     SPRED2 Is a Novel Regulator of Autophagy in Hepatocellular
            Carcinoma Cells and Normal Hepatocytes
  JOURNAL   Int J Mol Sci 25 (11), 6269 (2024)
   PUBMED   38892460
  REMARK    GeneRIF: SPRED2 Is a Novel Regulator of Autophagy in Hepatocellular
            Carcinoma Cells and Normal Hepatocytes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2872)
  AUTHORS   Wazin,F. and Lovicu,F.J.
  TITLE     Conditional Ablation of Spred1 and Spred2 in the Eye Lens
            Negatively Impacts Its Development and Growth
  JOURNAL   Cells 13 (4), 290 (2024)
   PUBMED   38391903
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2872)
  AUTHORS   Zhu,L., Chen,M., Wang,W., Zhu,J. and Wu,H.
  TITLE     microRNA-141-3p mediates epithelial cell proliferation, apoptosis,
            and epithelial-mesenchymal transition and alleviates pulmonary
            fibrosis in mice via Spred2
  JOURNAL   Histol Histopathol 38 (11), 1269-1282 (2023)
   PUBMED   36704943
  REMARK    GeneRIF: microRNA-141-3p mediates epithelial cell proliferation,
            apoptosis, and epithelial-mesenchymal transition and alleviates
            pulmonary fibrosis in mice via Spred2.
REFERENCE   5  (bases 1 to 2872)
  AUTHORS   Motta,M., Fasano,G., Gredy,S., Brinkmann,J., Bonnard,A.A.,
            Simsek-Kiper,P.O., Gulec,E.Y., Essaddam,L., Utine,G.E., Guarnetti
            Prandi,I., Venditti,M., Pantaleoni,F., Radio,F.C., Ciolfi,A.,
            Petrini,S., Consoli,F., Vignal,C., Hepbasli,D., Ullrich,M., de
            Boer,E., Vissers,L.E.L.M., Gritli,S., Rossi,C., De Luca,A., Ben
            Becher,S., Gelb,B.D., Dallapiccola,B., Lauri,A., Chillemi,G.,
            Schuh,K., Cave,H., Zenker,M. and Tartaglia,M.
  TITLE     SPRED2 loss-of-function causes a recessive Noonan syndrome-like
            phenotype
  JOURNAL   Am J Hum Genet 108 (11), 2112-2129 (2021)
   PUBMED   34626534
REFERENCE   6  (bases 1 to 2872)
  AUTHORS   Nobuhisa,I., Kato,R., Inoue,H., Takizawa,M., Okita,K., Yoshimura,A.
            and Taga,T.
  TITLE     Spred-2 suppresses aorta-gonad-mesonephros hematopoiesis by
            inhibiting MAP kinase activation
  JOURNAL   J Exp Med 199 (5), 737-742 (2004)
   PUBMED   14981116
  REMARK    GeneRIF: Spred-2 functions as a negative regulator of
            aorta-gonad-mesonephros hematopoiesis by inhibiting hematopoietic
            cytokine signaling.
REFERENCE   7  (bases 1 to 2872)
  AUTHORS   Kato,R., Nonami,A., Taketomi,T., Wakioka,T., Kuroiwa,A., Matsuda,Y.
            and Yoshimura,A.
  TITLE     Molecular cloning of mammalian Spred-3 which suppresses tyrosine
            kinase-mediated Erk activation
  JOURNAL   Biochem Biophys Res Commun 302 (4), 767-772 (2003)
   PUBMED   12646235
REFERENCE   8  (bases 1 to 2872)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 2872)
  AUTHORS   Wakioka,T., Sasaki,A., Kato,R., Shouda,T., Matsumoto,A.,
            Miyoshi,K., Tsuneoka,M., Komiya,S., Baron,R. and Yoshimura,A.
  TITLE     Spred is a Sprouty-related suppressor of Ras signalling
  JOURNAL   Nature 412 (6847), 647-651 (2001)
   PUBMED   11493923
REFERENCE   10 (bases 1 to 2872)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AL645609.21
            and AL672084.10.
            
            On May 1, 2007 this sequence version replaced NM_033523.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK036430.1, BC066013.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-515               AL645609.21        116758-117272
            516-693             AL672084.10        70943-71120
            694-862             AL672084.10        78605-78773
            863-927             AL672084.10        81236-81300
            928-1065            AL672084.10        90875-91012
            1066-2872           AL672084.10        93614-95420
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 12.79 cM"
     gene            1..2872
                     /gene="Spred2"
                     /note="sprouty-related EVH1 domain containing 2"
                     /db_xref="GeneID:114716"
                     /db_xref="MGI:MGI:2150019"
     exon            1..515
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
     CDS             490..1722
                     /gene="Spred2"
                     /note="sprouty protein with EVH-1 domain 2, related
                     sequence"
                     /codon_start=1
                     /product="sprouty-related, EVH1 domain-containing protein
                     2"
                     /protein_id="NP_277058.1"
                     /db_xref="CCDS:CCDS24454.1"
                     /db_xref="GeneID:114716"
                     /db_xref="MGI:MGI:2150019"
                     /translation="MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVC
                     KVMHPEGNGRSGFLIHGERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTF
                     QSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELGDDDVFTTATDSSSNSSQKRE
                     PTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEIVRINPREK
                     IWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPK
                     GSVIKTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSC
                     MWCADSMLYHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRAC
                     HRCGVMCRCCGGKHKAAA"
     exon            516..693
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
     exon            694..862
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
     exon            863..927
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1065
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..2872
                     /gene="Spred2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aatatggagt aaatcggtgg agtggcgaga tggggctcgg acggggcgcc ctgcaccgcc
       61 tcccaggcgc atctcccccg gccgccgagc actacccgga accctgattg gcccggtccc
      121 cgggggcgac cccggcccgg ccccagcgca gccgactgcc cgcccgccgc ccggagcgtg
      181 cttgtttttc tgctgctctc cccctccggg tccccttccc aaatctcgcc tctgcacccc
      241 ctcagagccc cggggccgtg cccggccggc ccacggcgtc cgctgcctgg tgtgcccctt
      301 cccagacctc gccctgatct cggggctctc catttccccg cctgcctcgg cgcccccttc
      361 atctctagct ccccccctcc accaccaaat caggagatgt cggagatgtg aagaaggggg
      421 gcgagcgcac aggaagatga agggagccag gctgcatgcc gcgggacagg cgtctaggtg
      481 aacaagaaaa tgaccgaaga aacacacccg gacgatgaca gctatattgt gcgtgtcaag
      541 gctgtggtta tgaccagaga tgactccagc gggggatggt tcccacagga aggaggcggg
      601 atcagtcgcg tcggcgtgtg taaggtcatg caccctgaag gcaacggacg aagcggcttt
      661 ctcatccatg gcgagcgaca gaaagacaaa ctggtggtat tggaatgcta tgtcagaaag
      721 gacttggtct acaccaaagc caatccgacg tttcatcatt ggaaggttga taacaggaag
      781 tttggactta ctttccaaag tcctgcagat gcacgagcct ttgacagggg cgtgagaaaa
      841 gccattgaag accttataga aggttcaacg acctcctctt ccactctcca taacgaagct
      901 gagctcggag acgatgacgt tttcacgaca gctacggaca gttcttctaa ttcctcgcag
      961 aagagggagc cgactacgag gacaatctcc tcccccacgt cctgtgagca ccggaagatt
     1021 tatacccttg acccataccc catggaccat taccaccctg accagcggtt gccgcggtcc
     1081 tacccccagg tcaccttccc agaagatgat gaagaaattg tacgcatcaa cccccgagag
     1141 aagatctgga tgaccggtta tgaagactac cggcacgcgc cggttcgcgg caaatactta
     1201 gacaccacag aagacgcgga ctcctacgtg cgcttcgcca agggcgaagt ccccaaacac
     1261 gaatatacct atccctatgt tgattcttcg gacttcggct tcggggagga tcccaaaggt
     1321 agtgtgatca agacacagcc gcccagggcc aagtcccgtc ggcggaagga gaacggcgaa
     1381 cggtcgcggt gtgtgtactg cagggatatg tttaatcacg aagagaaccg aaggggccac
     1441 tgccaagacg cgcccgacgc cgtgagaact tgcattcgcc gggtgagctg tatgtggtgc
     1501 gcggacagca tgctgtacca ctgtatgtcc gaccccgagg gagactacac tgacccttgt
     1561 tcgtgtgaca caagcgatga gaagttttgc ctccggtgga tggctctaat tgccttgtct
     1621 ttcctggccc cttgtatgtg ctgttacctg cccctccggg cctgccaccg ctgtggagtg
     1681 atgtgcaggt gctgtggtgg gaagcacaaa gccgccgcgt gactcggttc ccctactttc
     1741 tgcttctcca tccacggcca cgggcaactt gtctcttgcg cactccccca tcctctccct
     1801 ggttcccttc caactccaaa aggctaggcc aataagagga ggccaggctc tgtggtccct
     1861 tggtgacatc attccaaacc tggggtagct gccgccccct gtgtcagcca cataccccct
     1921 ctcgcagtgt tctgaggaaa ggagccttcg gcatagactc gtgtatttta ccagcctata
     1981 atcgtgctga gtgttggttt cctgcccctc cttcccacct ttctggttct gcagacttgg
     2041 aggggtttat tgactctccc cccccctccc ccgctctgtg actttttttt ttttcttctc
     2101 cccattgggt ttgcctgtcg ctaactagca taggtcccac agagatgtga cagaatgatg
     2161 aagccggcac cacagcatgt tgttatgtga ggaggcaatc tggatagcaa gcatgtacca
     2221 caaaacagta gtactttaac ccctaatgcc acgtctcccc atcagctgcc ggttttgtct
     2281 ttaacctgct atcatctctt ctcctgcccc tccctcccca tgaattaatt cactaatgca
     2341 aaacaatcac tttttaaatg actttttgag ccttaaatcc atattcactt gtgagaatca
     2401 ggaaagaatt cctcgagttg ctttgcatcc tcttggacca gggcttgcaa ggcgatttgg
     2461 gtttctgtga gcttgcccag ttgacccgaa aaataggact ttttagaatt gcattgccac
     2521 ttctaaaatc ctagcaatac ttagaataac acatcagtga gataaatttt actcaattat
     2581 tttacaaacc aaattgagtt attcaaacac aagccagtat tctgtgaaca agttccagat
     2641 gaaaaacaca gaagttgtga atgaagttgt agccattccc tgccctgcac tatatttttc
     2701 agggtgcatt ttaaatacta ttgattcaaa cagtggtggc ttgcgttttt ttaggtatat
     2761 atttttggtt agttttttgg ggggggttgt tttgtttttt taatttgagg ctcaattctt
     2821 catttatact tgaaaaaaac aaggccatat gtaaagaaaa aaaaaaaccc tc
//
