LOCUS       NM_033524               6295 bp    mRNA    linear   ROD 18-JUN-2024
DEFINITION  Mus musculus sprouty protein with EVH-1 domain 1, related sequence
            (Spred1), transcript variant 1, mRNA.
ACCESSION   NM_033524
VERSION     NM_033524.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Wazin,F. and Lovicu,F.J.
  TITLE     Conditional Ablation of Spred1 and Spred2 in the Eye Lens
            Negatively Impacts Its Development and Growth
  JOURNAL   Cells 13 (4), 290 (2024)
   PUBMED   38391903
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6295)
  AUTHORS   Qiao,J., Zhao,D., Nguyen,L.X.T., Chen,F., Liang,C., Estrella,K.,
            Ghoda,L.Y., Heisterkamp,N., Marcucci,E.C., Kuo,Y.H., Marcucci,G.
            and Zhang,B.
  TITLE     Targeting miR-126 in Ph+ acute lymphoblastic leukemia
  JOURNAL   Leukemia 37 (7), 1540-1544 (2023)
   PUBMED   37296274
REFERENCE   4  (bases 1 to 6295)
  AUTHORS   Qiao,J., Liang,C., Zhao,D., Nguyen,L.X.T., Chen,F., Suo,S.,
            Hoang,D.H., Pellicano,F., Rodriguez,I.R., Elhajmoussa,Y., Ghoda,L.,
            Yoshimura,A., Stein,A.S., Ali,H., Koller,P., Perrotti,D.,
            Copland,M., Han,A., Zhang,B.A. and Marcucci,G.
  TITLE     Spred1 deficit promotes treatment resistance and transformation of
            chronic phase CML
  JOURNAL   Leukemia 36 (2), 492-506 (2022)
   PUBMED   34564700
  REMARK    GeneRIF: Spred1 deficit promotes treatment resistance and
            transformation of chronic phase CML.
REFERENCE   5  (bases 1 to 6295)
  AUTHORS   Guy,G.R., Jackson,R.A., Yusoff,P. and Chow,S.Y.
  TITLE     Sprouty proteins: modified modulators, matchmakers or missing
            links?
  JOURNAL   J Endocrinol 203 (2), 191-202 (2009)
   PUBMED   19423641
  REMARK    Review article
REFERENCE   6  (bases 1 to 6295)
  AUTHORS   Nonami,A., Kato,R., Taniguchi,K., Yoshiga,D., Taketomi,T.,
            Fukuyama,S., Harada,M., Sasaki,A. and Yoshimura,A.
  TITLE     Spred-1 negatively regulates interleukin-3-mediated
            ERK/mitogen-activated protein (MAP) kinase activation in
            hematopoietic cells
  JOURNAL   J Biol Chem 279 (50), 52543-52551 (2004)
   PUBMED   15465815
  REMARK    GeneRIF: role in hematopoiesis
REFERENCE   7  (bases 1 to 6295)
  AUTHORS   Engelhardt,C.M., Bundschu,K., Messerschmitt,M., Renne,T.,
            Walter,U., Reinhard,M. and Schuh,K.
  TITLE     Expression and subcellular localization of Spred proteins in mouse
            and human tissues
  JOURNAL   Histochem Cell Biol 122 (6), 527-538 (2004)
   PUBMED   15580519
  REMARK    GeneRIF: Spred-1 and Spred-2 were found to be expressed
            predominantly in brain
REFERENCE   8  (bases 1 to 6295)
  AUTHORS   Miyoshi,K., Wakioka,T., Nishinakamura,H., Kamio,M., Yang,L.,
            Inoue,M., Hasegawa,M., Yonemitsu,Y., Komiya,S. and Yoshimura,A.
  TITLE     The Sprouty-related protein, Spred, inhibits cell motility,
            metastasis, and Rho-mediated actin reorganization
  JOURNAL   Oncogene 23 (33), 5567-5576 (2004)
   PUBMED   15184877
  REMARK    GeneRIF: These data suggest that Spreds are key regulators of
            RhoA-mediated cell motility and signal transduction. Furthermore,
            our study suggests that the induction of Spreds could be a novel
            strategy for preventing cancer cell metastasis.
REFERENCE   9  (bases 1 to 6295)
  AUTHORS   Kato,R., Nonami,A., Taketomi,T., Wakioka,T., Kuroiwa,A., Matsuda,Y.
            and Yoshimura,A.
  TITLE     Molecular cloning of mammalian Spred-3 which suppresses tyrosine
            kinase-mediated Erk activation
  JOURNAL   Biochem Biophys Res Commun 302 (4), 767-772 (2003)
   PUBMED   12646235
REFERENCE   10 (bases 1 to 6295)
  AUTHORS   Wakioka,T., Sasaki,A., Kato,R., Shouda,T., Matsumoto,A.,
            Miyoshi,K., Tsuneoka,M., Komiya,S., Baron,R. and Yoshimura,A.
  TITLE     Spred is a Sprouty-related suppressor of Ras signalling
  JOURNAL   Nature 412 (6847), 647-651 (2001)
   PUBMED   11493923
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL807741.8 and AL928959.9.
            
            On Oct 27, 2022 this sequence version replaced NM_033524.3.
            
            Summary: This gene encodes a membrane-associated protein that is
            phosphorylated by tyrosine kinases in response to growth factors.
            The encoded protein acts as a negative regulator of the
            mitogen-activated protein (MAP) kinase signaling pathway.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Mar 2013].
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK155065.1, BC079606.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-662               AL807741.8         46849-47510         c
            663-837             AL807741.8         15439-15613         c
            838-1006            AL807741.8         4939-5107           c
            1007-1056           AL807741.8         3610-3659           c
            1057-1215           AL928959.9         5324-5482
            1216-1317           AL928959.9         8740-8841
            1318-6295           AL928959.9         10698-15675
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 59.03 cM"
     gene            1..6295
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
                     /note="sprouty protein with EVH-1 domain 1, related
                     sequence"
                     /db_xref="GeneID:114715"
                     /db_xref="MGI:MGI:2150016"
     exon            1..662
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            663..837
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            838..1006
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1056
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1215
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1317
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..6295
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4279..4284
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      4300
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
                     /note="major polyA site"
     regulatory      6270..6275
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      6295
                     /gene="Spred1"
                     /gene_synonym="5730461F13Rik"
ORIGIN      
        1 gcggctcccc tctcgcgggc gtgaggagcg ggccgccgag cagcgctgat ctcggccctc
       61 gtcgccgccg ctcggcactg tgccctcggg ctccgacgct cgcctcgcgc cctgcctgcg
      121 gcccctcagc tggagcgcgt ccgaggcagc ccctgagggc cgaccaggcg cgccgcgcgg
      181 gcagccggcg gccaggcggg agatggtgcg cggcacctcg gcgggaccgc gccgcctccc
      241 gcagccagcg ctcggctgcc ccccgccggc tcccgccgcc cgcccgtcgc cgcgcccctg
      301 agagcagcac cgcctcagcc accggccgcg tccccctcag cgcccgcagt cgcccgcgcc
      361 gtctcggctt cgcactcgac ctcggcgcgg caaaccgctg gggtcgccac ggccgcgctt
      421 gctgccgccg cccccctgcg ggggtggccg ggctgcccgg ctcgcggggc accgctccga
      481 gtgaggcatc caccatggtg aggcccccga gccgctgccc gcgcgccccg ccggccgtcg
      541 tcgcccgccg cccctcggtg ccgccgctgc cgctgcttcc ctgcctgctg ttgctcctcc
      601 atctcctgat cggatcacgg tgagggaaag atgagcgagg agacggcgac ttctgacaac
      661 gataatagtt atgcacgagt gcgagctgtg gtgatgacca gagatgactc aagtggtgga
      721 tggctaccac tcggagggag cggactgagc agcgtcactg tcttcagagt ccctcatcag
      781 gaagagaatg gctgtgcgga cttttttatc cgtggagaga gactcagaga caaaatggtg
      841 gttttggaat gtatgcttaa gaaagacctc atctataata aggtcactcc cacatttcac
      901 cactggaaga tcgatgacaa gaagtttggc cttacctttc agagtcctgc tgatgccagg
      961 gcttttgatc gaggcattcg aagagctata gaggatatat ctctagggtg cccagcgtca
     1021 aaaactgagg ctgaaggagg agatgatgat ttacaaacga ctgaagaaga cacttcccgt
     1081 tccctagtga aagatcactt tttccagcaa gagacagttg ttaccagtga accttacaga
     1141 agctcagaca taagaccgtt accctttgaa gatctgaatg ccagaagagt ctacttgcaa
     1201 agccaagtca gccagatacc attcagtcag caaggcctag acattcagag ccgaagtatg
     1261 gaatatgtac agcggcaaat atctaaggaa tgtgggagcc taaagtccca aactagggtc
     1321 cctttgaagt caatcagaca tgtcagcttt caagatgagg atgagattgt tagaataaat
     1381 cctcgagata tcttaatacg tcgatatgca gactacagac atccggacat gtggaaaaat
     1441 gacttggaga gagatgatac tgactccagt gttccatttt ctaaacaaga cagtaaaaag
     1501 tcagactatc tctaccactg tggggatgag actaagttaa gttctctgaa agactctgtg
     1561 gtatttaaga cacagcctcc ctcgttaaaa tttaagtcaa aacgaagaaa agaggatggg
     1621 gaacgttctc gctgcgtata ctgccaggaa aggtttaatc atgaagaaaa cgccaggggg
     1681 aaatgccagg acgctccaga tcctgttaaa agatgcatat atcaagttag ctgcatgctc
     1741 tgtgctgaga gcatgctata tcattgtatg tcagactcag agggagactt ttctgatcct
     1801 tgttcgtgtg acactagcga tgacaagttc tgcctaaggt ggttagccct ggtagctctg
     1861 tctttcattg taccatgtat gtgctgctac gtccctttga gaatgtgcca tcgctgtggt
     1921 gaggcatgcg ggtgctgtgg tgggaaacat aaggctgctg ggtgaagcag tccagggccg
     1981 gcatgagctt aaaccttcat ctccaggaat tagctaactt ggatttgtgg aagctgttgg
     2041 caagcaatat ggaatcttgc cggtatcatt ggggccatgc gtggaggaag cagaactcat
     2101 cagttcttgt gatttacaga tgctagccgt gcctgtttcc ctgagtgcat ggcatgttct
     2161 gggacagatc acagagcatt ttcagaaggc agtcatttct ggttattgat tacaaaaggt
     2221 caacatagaa taggatccaa ctaaaaggga ttaattttgg cattttcata tttatgcact
     2281 aggtgatggg actttttaaa gatttgaatt ctttaggaca ttaactaaaa agtgcactaa
     2341 gggacagtga tttcagtaca gaaaagttag tacttgggaa attaagagat aagacaagtg
     2401 caactaaatc attggttgtt ttttgaaagc agttttatgt ataaataaca aatgtttata
     2461 tttaactaaa tgtaaggtac gaactatgac atattaaact tttcttgccc ttcctagttc
     2521 tgaggtagat gtgcacatat ctgccaccac attccatatg tgttgagtag ttaactcatc
     2581 tagttaataa cgtttgtgtc catgtgagag attcggtgag ttcatcctca gctctcttgg
     2641 gctgcagttt atattgagtc atatctgtac attctggaga tctaaaatct ttaaaatact
     2701 ctaatagcct tgagtgacca accttttttt ttaaagcaca gatgtaattg tctaatgttc
     2761 tgatggaaat gtaacactta tttttatata aagagattga gtaaacagaa gaaattgagg
     2821 gttcctttag ttgttttgtt tctgtggtat tcagccagca agttgttttc tttcagagtt
     2881 tcctccttca gagaacttaa ctgcatttat aaacgtttta caaggcagaa gcattaactg
     2941 gatagttagt gtgaaacagt ttccgcttga gatctccatt tctcactctg ctgagccaga
     3001 actcgcacag ctgtttcact gattttctgt gcttattact cacataacaa tggtaattaa
     3061 tacctttcag ttgcatttta ttatatttca ggagcaaagt tactttgcag taacacatat
     3121 gcagaattct cccaggaaag tattcaatcc accattgtga atggaaatct tatacaccac
     3181 attcctgtcg cagagcattt gtattatggg atgtttgatt tacaaagtag tgtgatatgc
     3241 tccagcaaac aggttaaaaa tactttgagg tcaaattgtc ttggttttgt ttttttaaag
     3301 tgttactatt aaaactcttg tgagggattg ttgcaaaata ctccctcagg aaagaaagca
     3361 gtgtgccttt tttgcctttt ttttttaatg gattaaagtt tctgaagtga taaaaatgaa
     3421 aatcacacta ccatttgaag tccattctcc aggttttcag tgtcatttct gtgcagtcag
     3481 ttttatgtgt cacacttcgg cttgtgttgg ctttcttctt ttgccccaga ttaaactgaa
     3541 caatgtatat atatataaca ctatctgtct gtaaaatact ttttttaaga aagcatttat
     3601 atttatatga cagcttgaac tgacaacatt atgtatatag atcatcttga agtattattt
     3661 cacattgaaa agaagaaaaa tatattgata actatagatg ttatgaagaa gaggctattt
     3721 ctagttttgt actaaaaatc aattggatga actaaatcca aaaccagaca ctgtcgcagc
     3781 aattttcagt cttattttta ctgtttctac attggaggct gctgcacaga tagatgatct
     3841 tcgtccttgc tgttttcaga ggagcttggt ttcctagagc agactgttac ctttcaggat
     3901 tcctttttct tgctgaagta gagtcctggt tttccttatg agtgattatc catatttgta
     3961 agttctgggt ttgtaattca ggttggagca aggtgtgaat aactaaagaa aataacttgc
     4021 tggcaatata ggaaatgcca tggaaataaa ctgtgtatat acttctgaga agaacaaaat
     4081 taatcatttt ttttttctgt taagcactag tcactatagt gcatctcctg gttctgtact
     4141 gtattttata cgcaacgtac atgctttaat attttaatgt ttgtgcatta atattttcag
     4201 tttgtttaac cactttgctg ctaagatttt gccatcccgt tcccattttt tcctttacaa
     4261 ttttaaacat ttctcttcat taaaaactat gataatgaaa ttgttttttt tttaaccttg
     4321 gatttttgta attagcacac atgaattcaa gccagtccag aaaagaaact gatgatataa
     4381 tgttaatgag tctctttgtg aattagatgg acagtttaat ccaattccca acatgtacgt
     4441 gtaggtggat tcaagaatga tttcttgttc actgtacaga ttgggcgctg ccattgggca
     4501 ttttctttaa aacttctctt ttgtctagaa tgtaagcaga tagctcaaca aaactggtga
     4561 agtaaaagta aatattgaaa atgaaatcat ctgaatagcg tgacccaaaa attctccagc
     4621 tggggggcct gtttgctcct gatctgtagt tctcagagga tgcttgcagc ctgtggggaa
     4681 gccttagatt tcctgagcag tgcttgcgga gctgcagtgc cacctgctgg atggctttag
     4741 accagcgctg aaagatggaa atggtgtctc cagtaaagga gggctgtggg ctgttcaggg
     4801 tggccagtgc ccaccagaga aatcgggggt gaggaaaagc gctaaggagt gtaagtaagt
     4861 ctttccccaa atgcatatgg ttgctggaag aattgaagca ctttttcctt acacatcctg
     4921 ttttaaaata tttattgaaa cttggaactg gcaatcaaac ttatgtatgt actgaagcat
     4981 ttatacaacc agtttcttca actcagaagc cattaccaaa atggtattcc tattttcttc
     5041 ccagtaccag tcagggagaa ttaaaaaaaa aataaacata attacctgta ttttatcatg
     5101 acaactccag agagtgtgta tgtttctgag tagatgttga tgaggcagga cttaaaattt
     5161 aaagagaaaa acttgaaact acctattttc attggttatt cagcagtgcc taaaatgagc
     5221 ttctgaacta ttacaaatgc actttggatt catgtgtaca gtgccatctg gttctctggg
     5281 tcaaacttgc ttctcctctc cctctttctt ctcccgtaac taagtggcac acaggcattt
     5341 cttagtgttc gtgtcggtct tcaaagtgca gagaacgtgg tggagagaat tttatggatc
     5401 ttttttttct gttaaaattg aatgtctgat tatatttgca acatgtaact tggattaatg
     5461 tgttttaaag taattttaat tgtagtccag cagcacattg agcaagtgta gcttttctga
     5521 aaagtaactt ggagaacttc agttaaccga taaaaccaac tcatcactaa atgtcgctgc
     5581 tcttcctctt gatttgatct agatcccact tctgcgtctt acagggaaca ccaaaaagtt
     5641 gctagtaaat gatagtagag aaatagaggg agtcaaacag aaaatattaa aaatatgcgc
     5701 acacagacaa acagaggtga tttatacaaa ccagaatgaa ggtggggagg gaaggaaaat
     5761 tacagggggc tttaaaaggt accagttacc tagcagctgg gcaattagaa acctttcagt
     5821 tgctttggta cactgtcatc cattgttccc aaaagctaaa tttgcaaaag caattagttt
     5881 tatttcattt tttcaagaat ttcctagtcg aaagcctttg tattaaagat gcacaggttt
     5941 ttgtgtgaac aggttttttt gtgaacaagt atgttgtaca tttttgctgt tatcttgacc
     6001 tcagaagtgc ctcacttgta tattacccac cccaccccca cccccactag gagcctgcct
     6061 gcatctttct aataagcaat ggaagcccgc ttaactcctg tcctaatgca tcttctcaca
     6121 tagagctgac tggctgagag ttgtgagtat atcatgtaaa tacgcacata ggagtttgta
     6181 actgcttttg ttctgtcata tcatgtaatt ggacgtatca gatggtagac tctttgtttc
     6241 agtgatttct cctcccctgg aattacctga ttaaaccaaa ttgtgaaatt taaaa
//
