LOCUS       NM_029012               2817 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus signal peptide peptidase 3 (Sppl3), mRNA.
ACCESSION   NM_029012 NM_031400 XM_485673
VERSION     NM_029012.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Hamblet,C.E., Makowski,S.L., Tritapoe,J.M. and Pomerantz,J.L.
  TITLE     NK Cell Maturation and Cytotoxicity Are Controlled by the
            Intramembrane Aspartyl Protease SPPL3
  JOURNAL   J Immunol 196 (6), 2614-2626 (2016)
   PUBMED   26851218
  REMARK    GeneRIF: Our results identify SPPL3 as a cell-autonomous molecular
            determinant of NK cell maturation
REFERENCE   3  (bases 1 to 2817)
  AUTHORS   Kuhn,P.H., Voss,M., Haug-Kroper,M., Schroder,B., Schepers,U.,
            Brase,S., Haass,C., Lichtenthaler,S.F. and Fluhrer,R.
  TITLE     Secretome analysis identifies novel signal Peptide peptidase-like 3
            (Sppl3) substrates and reveals a role of Sppl3 in multiple Golgi
            glycosylation pathways
  JOURNAL   Mol Cell Proteomics 14 (6), 1584-1598 (2015)
   PUBMED   25827571
REFERENCE   4  (bases 1 to 2817)
  AUTHORS   Makowski,S.L., Wang,Z. and Pomerantz,J.L.
  TITLE     A protease-independent function for SPPL3 in NFAT activation
  JOURNAL   Mol Cell Biol 35 (2), 451-467 (2015)
   PUBMED   25384971
REFERENCE   5  (bases 1 to 2817)
  AUTHORS   Voss,M., Kunzel,U., Higel,F., Kuhn,P.H., Colombo,A., Fukumori,A.,
            Haug-Kroper,M., Klier,B., Grammer,G., Seidl,A., Schroder,B.,
            Obst,R., Steiner,H., Lichtenthaler,S.F., Haass,C. and Fluhrer,R.
  TITLE     Shedding of glycan-modifying enzymes by signal peptide
            peptidase-like 3 (SPPL3) regulates cellular N-glycosylation
  JOURNAL   EMBO J 33 (24), 2890-2905 (2014)
   PUBMED   25354954
REFERENCE   6  (bases 1 to 2817)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   7  (bases 1 to 2817)
  AUTHORS   Jensen,P., Magdaleno,S., Lehman,K.M., Rice,D.S., Lavallie,E.R.,
            Collins-Racie,L., McCoy,J.M. and Curran,T.
  TITLE     A neurogenomics approach to gene expression analysis in the
            developing brain
  JOURNAL   Brain Res Mol Brain Res 132 (2), 116-127 (2004)
   PUBMED   15582152
REFERENCE   8  (bases 1 to 2817)
  AUTHORS   Wertz,K. and Herrmann,B.G.
  TITLE     Large-scale screen for genes involved in gonad development
  JOURNAL   Mech Dev 98 (1-2), 51-70 (2000)
   PUBMED   11044607
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BU613738.1, CD806412.1, AK014709.1 and CAAA01026347.1.
            
            On or before Oct 4, 2005 this sequence version replaced
            NM_031400.1, XM_485673.2, NM_029012.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC086646.1, AK014709.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-571               BU613738.1         114-684
            572-1107            CD806412.1         189-724
            1108-1778           AK014709.1         1446-2116
            1779-2817           CAAA01026347.1     7978-9016
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 55.99 cM"
     gene            1..2817
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /note="signal peptide peptidase 3"
                     /db_xref="GeneID:74585"
                     /db_xref="MGI:MGI:1891433"
     exon            1..92
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..1224
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /note="upregulated during skeletal muscle growth 3; IMP-2;
                     SPP-like 3 protein; intramembrane protease 2;
                     presenilin-like protein 4"
                     /codon_start=1
                     /product="signal peptide peptidase-like 3"
                     /protein_id="NP_083288.2"
                     /db_xref="CCDS:CCDS19578.1"
                     /db_xref="GeneID:74585"
                     /db_xref="MGI:MGI:1891433"
                     /translation="MAEQTYSWAYSLVDSSQVSTFLISILLIVYGSFRSLNMDFENQD
                     KEKDSNSSSGSFNGNSTNNSIQTIDSTQALFLPIGASVSLLVMFFFFDSVQVVFTICT
                     AVLATIAFAFLLLPMCQYLTRPCSPQNKISFGCCGRFTAAELLSFSLSVMLVLIWVLT
                     GHWLLMDALAMGLCVAMIAFVRLPSLKVSCLLLSGLLIYDVFWVFFSAYIFNSNVMVK
                     VATQPADNPLDVLSRKLHLGPNVGRDVPRLSLPGKLVFPSSTGSHFSMLGIGDIVMPG
                     LLLCFVLRYDNYKKQASGDSCGAPGPANISGRMQKVSYFHCTLIGYFVGLLTATVASR
                     IHRAAQPALLYLVPFTLLPLLTMAYLKGDLRRMWSEPFHSKSSSSRFLEV"
     exon            93..170
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /inference="alignment:Splign:2.1.0"
     exon            171..259
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /inference="alignment:Splign:2.1.0"
     exon            260..379
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /inference="alignment:Splign:2.1.0"
     exon            380..458
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /inference="alignment:Splign:2.1.0"
     exon            459..571
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /inference="alignment:Splign:2.1.0"
     exon            572..678
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /inference="alignment:Splign:2.1.0"
     exon            679..842
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /inference="alignment:Splign:2.1.0"
     exon            843..1042
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1152
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..2817
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2263..2268
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /note="hexamer: AATACA"
     polyA_site      2281
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /note="major polyA site"
     regulatory      2797..2802
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /note="hexamer: AATAAA"
     polyA_site      2817
                     /gene="Sppl3"
                     /gene_synonym="4833416I09Rik; Psl4; Usmg3"
                     /note="major polyA site"
ORIGIN      
        1 cgccggggcc cccggccgcg ccctctgccg ccggccgagc gagccgcgga gcccccgaga
       61 ggcagcacca tggcggaaca gacctattcg tgggcctatt ccctggtgga ctccagtcaa
      121 gtgtcgacat ttctgatatc cattcttctt atagtctatg gtagtttcag gtctcttaat
      181 atggactttg aaaaccaaga taaggagaaa gacagcaaca gttcttctgg ctctttcaat
      241 ggcaacagca ccaataacag catccagacc attgattcca cccaagcact gttcctcccg
      301 attggagcgt ctgtctctct cctcgtcatg ttcttcttct ttgattcagt tcaagtcgtt
      361 ttcacaatat gtacagcagt tcttgcaaca atagcttttg cttttcttct tctcccgatg
      421 tgccagtatt taacaaggcc ctgctcacct cagaacaaga tttccttcgg ttgctgtggg
      481 cgtttcactg ctgccgagct gctgtcgttc tccctgtctg tcatgctcgt cctcatctgg
      541 gttctcactg gccactggct tctcatggat gctctggcca tgggtctctg tgttgccatg
      601 atcgccttcg tccgcctgcc aagcctcaag gtttcctgcc tgcttctctc agggcttctc
      661 atctacgatg tcttctgggt gttcttctca gcctacatct tcaacagtaa tgtcatggtg
      721 aaagtggcca cacagccagc tgacaatccc ctcgacgttc tgtccaggaa gctccacctg
      781 ggacccaatg tggggcgtga tgttcctcgc ctgtctttgc ctggaaaatt ggtcttccca
      841 agctccactg gcagtcactt ctctatgctg ggcatcgggg acattgtgat gcccggcctc
      901 ctgttatgct ttgttcttcg ctatgacaac tacaagaaac aagccagtgg tgactcgtgt
      961 ggggcccctg gcccagccaa tatctctggg cgcatgcaga aggtctccta cttccactgc
     1021 accctcatcg ggtactttgt aggtctgctc actgcgactg tggcgtctcg catccaccga
     1081 gctgcccagc cagctctcct ctacttggtg ccatttaccc tattgccact cctcaccatg
     1141 gcctacctaa agggtgactt acggaggatg tggtctgagc ccttccactc caagtccagc
     1201 agctcccggt tcctggaagt atgatgacgc gggagaggtg accagagcgc tgtcctcacc
     1261 ttcctctctc accgcgcggt tttgttccct ctacaagctg gcctgactca ggcatgcctg
     1321 ctcacggaac tgcagtgtga ctggagtgtg tgtctgaggg gagcgtttgc agagggcact
     1381 ggagccctgc acagcccttc tcttctccgc tctgggagag tggacccttc cagaaggggc
     1441 aggccggccc ttcgcccggt gctcctcctt cctcgtgttt ttatggatct gcaccagact
     1501 gttaacttgt ggggagatgg cgaagttgac ttatttaaaa actacaaaca gcaagttgtt
     1561 gttctagaac tttgaacact aaatggaaga aaatccatta gcagaccgaa gtctcttcag
     1621 tgaaccctcc gaaactctgg gaccagtttc ctctgggggc tccatacaga gacccagggc
     1681 agaacttctg ccctattttt tttctttttt cttttggatt tattaaatat tttctgtggt
     1741 gtgaagtgac ttattacatc cacagacatt gaatgacttc ttacaacata cacataagaa
     1801 tttgttgtaa tgactgtgtc cagccgtgtt ggcagtgagc aggacgcggt tggtttatac
     1861 atacgtacat atatatatat atatacacac acgtgcacat agatatctgt gagtaaacag
     1921 cgactaagcc tgctaaggtc acgccatgca gctgccgggg gcttgctgtc atgggagcat
     1981 gtagaacagt ttacctggct tcctcgcaca cgctgctgtc tcccttgaga gtcgcgtgcg
     2041 gttgtaagct gtggatgcgc tgaactgata gacttgtggg ctgtggggac tcctcctttt
     2101 gatcagtgta gcaaattagg gatgaaaagt ttgaactttt tgaccctttt ctttttacag
     2161 gcttttccct cgcagttttt agtaaccgcc tcttgaacca gtgcatgtat tatagcagcc
     2221 ggtgtctttg tgctttctga tcatagttat gtactacctg taaatacatt tttctatttt
     2281 atattttttt gtattttttt tgacatttcg tttcattggt gcgctgtatt ttccatgccc
     2341 tcagtccctt agaaaagaca aaacagaaaa aagcaacgca actctgtcct tgctgtgatg
     2401 atcacagcct cagtttacct gtggtgacag caggctgtgg ggacaaatgt cagttgcctc
     2461 tgcaaatgga gctgaagtcc aggagctgga gacagagcag gctgctgctg tgacttcaaa
     2521 acctggcccc gcgctgttgc tgtccgtggg ctgcacagag cctggcatca ccatcacctc
     2581 acacttggtt agctgctgcc tgggtgcgag gcggcaggcc ctagagtctg ttccttcccg
     2641 gggccgctgg catcgtggcc tgcctgtccc tattcctgca gacattgtgc agagtgcaca
     2701 gcccatcgtg tccatggagc agcgtgccac caagttgttg gttcctctgt cgtcttcaga
     2761 ctctgttgag gggtttgaag ggttggtttt gtctctaata aatgactcct ttttcta
//
