LOCUS       NM_023220               5629 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus signal peptide peptidase like 2A (Sppl2a), mRNA.
ACCESSION   NM_023220
VERSION     NM_023220.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Ballin,M., Griep,W., Patel,M., Karl,M., Mentrup,T.,
            Rivera-Monroy,J., Foo,B., Schwappach,B. and Schroder,B.
  TITLE     The intramembrane proteases SPPL2a and SPPL2b regulate the
            homeostasis of selected SNARE proteins
  JOURNAL   FEBS J 290 (9), 2320-2337 (2023)
   PUBMED   36047592
  REMARK    GeneRIF: The intramembrane proteases SPPL2a and SPPL2b regulate the
            homeostasis of selected SNARE proteins.
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Gradtke,A.C., Mentrup,T., Lehmann,C.H.K., Cabrera-Cabrera,F.,
            Desel,C., Okakpu,D., Assmann,M., Dalpke,A., Schaible,U.E.,
            Dudziak,D. and Schroder,B.
  TITLE     Deficiency of the Intramembrane Protease SPPL2a Alters
            Antimycobacterial Cytokine Responses of Dendritic Cells
  JOURNAL   J Immunol 206 (1), 164-180 (2021)
   PUBMED   33239420
  REMARK    GeneRIF: Deficiency of the Intramembrane Protease SPPL2a Alters
            Antimycobacterial Cytokine Responses of Dendritic Cells.
REFERENCE   3  (bases 1 to 5629)
  AUTHORS   Mentrup,T., Theodorou,K., Cabrera-Cabrera,F., Helbig,A.O., Happ,K.,
            Gijbels,M., Gradtke,A.C., Rabe,B., Fukumori,A., Steiner,H.,
            Tholey,A., Fluhrer,R., Donners,M. and Schroder,B.
  TITLE     Atherogenic LOX-1 signaling is controlled by SPPL2-mediated
            intramembrane proteolysis
  JOURNAL   J Exp Med 216 (4), 807-830 (2019)
   PUBMED   30819724
  REMARK    GeneRIF: SPPL2a/b negatively regulates LOX-1 signaling and
            atherosclerosis.
REFERENCE   4  (bases 1 to 5629)
  AUTHORS   Papadopoulou,A.A., Muller,S.A., Mentrup,T., Shmueli,M.D.,
            Niemeyer,J., Haug-Kroper,M., von Blume,J., Mayerhofer,A.,
            Feederle,R., Schroder,B., Lichtenthaler,S.F. and Fluhrer,R.
  TITLE     Signal peptide peptidase-like 2c impairs vesicular transport and
            cleaves SNARE proteins
  JOURNAL   EMBO Rep 20 (3) (2019)
   PUBMED   30733281
  REMARK    Erratum:[EMBO Rep. 2019 May;20(5):e48133. doi:
            10.15252/embr.201948133.. PMID: 31061204]
REFERENCE   5  (bases 1 to 5629)
  AUTHORS   Kong,X.F., Martinez-Barricarte,R., Kennedy,J., Mele,F., Lazarov,T.,
            Deenick,E.K., Ma,C.S., Breton,G., Lucero,K.B., Langlais,D.,
            Bousfiha,A., Aytekin,C., Markle,J., Trouillet,C., Jabot-Hanin,F.,
            Arlehamn,C.S.L., Rao,G., Picard,C., Lasseau,T., Latorre,D.,
            Hambleton,S., Deswarte,C., Itan,Y., Abarca,K.,
            Moraes-Vasconcelos,D., Ailal,F., Ikinciogullari,A., Dogu,F.,
            Benhsaien,I., Sette,A., Abel,L., Boisson-Dupuis,S., Schroder,B.,
            Nussenzweig,M.C., Liu,K., Geissmann,F., Tangye,S.G., Gros,P.,
            Sallusto,F., Bustamante,J. and Casanova,J.L.
  TITLE     Disruption of an antimycobacterial circuit between dendritic and
            helper T cells in human SPPL2a deficiency
  JOURNAL   Nat Immunol 19 (9), 973-985 (2018)
   PUBMED   30127434
  REMARK    GeneRIF: Sppl2a-/- mice lack conventional dendritic cells, have
            CD4+ T cells that produce small amounts of IFN-gamma after BCG
            infection, and are highly susceptible to infection with BCG or
            Mycobacterium tuberculosis
REFERENCE   6  (bases 1 to 5629)
  AUTHORS   Bronckers,A.L., Gueneli,N., Lullmann-Rauch,R., Schneppenheim,J.,
            Moraru,A.P., Himmerkus,N., Bervoets,T.J., Fluhrer,R., Everts,V.,
            Saftig,P. and Schroder,B.
  TITLE     The intramembrane protease SPPL2A is critical for tooth enamel
            formation
  JOURNAL   J Bone Miner Res 28 (7), 1622-1630 (2013)
   PUBMED   23426979
  REMARK    GeneRIF: intramembrane proteolysis by SPPL2A is essential for
            maintaining cellular homeostasis of ameloblasts.
REFERENCE   7  (bases 1 to 5629)
  AUTHORS   Bergmann,H., Yabas,M., Short,A., Miosge,L., Barthel,N., Teh,C.E.,
            Roots,C.M., Bull,K.R., Jeelall,Y., Horikawa,K., Whittle,B.,
            Balakishnan,B., Sjollema,G., Bertram,E.M., Mackay,F., Rimmer,A.J.,
            Cornall,R.J., Field,M.A., Andrews,T.D., Goodnow,C.C. and Enders,A.
  TITLE     B cell survival, surface BCR and BAFFR expression, CD74 metabolism,
            and CD8- dendritic cells require the intramembrane endopeptidase
            SPPL2A
  JOURNAL   J Exp Med 210 (1), 31-40 (2013)
   PUBMED   23267016
  REMARK    GeneRIF: B cell survival and dendritic cells function requires
            SPPL2A.
REFERENCE   8  (bases 1 to 5629)
  AUTHORS   Schneppenheim,J., Dressel,R., Huttl,S., Lullmann-Rauch,R.,
            Engelke,M., Dittmann,K., Wienands,J., Eskelinen,E.L.,
            Hermans-Borgmeyer,I., Fluhrer,R., Saftig,P. and Schroder,B.
  TITLE     The intramembrane protease SPPL2a promotes B cell development and
            controls endosomal traffic by cleavage of the invariant chain
  JOURNAL   J Exp Med 210 (1), 41-58 (2013)
   PUBMED   23267015
  REMARK    GeneRIF: Sppl2a promotes B cell development and controls endosomal
            traffic by cleavage of the invariant chain.
REFERENCE   9  (bases 1 to 5629)
  AUTHORS   Beisner,D.R., Langerak,P., Parker,A.E., Dahlberg,C., Otero,F.J.,
            Sutton,S.E., Poirot,L., Barnes,W., Young,M.A., Niessen,S.,
            Wiltshire,T., Bodendorf,U., Martoglio,B., Cravatt,B. and Cooke,M.P.
  TITLE     The intramembrane protease Sppl2a is required for B cell and DC
            development and survival via cleavage of the invariant chain
  JOURNAL   J Exp Med 210 (1), 23-30 (2013)
   PUBMED   23267013
  REMARK    GeneRIF: Sppl2a is required for B cell and dendritic cells
            development and survival.
REFERENCE   10 (bases 1 to 5629)
  AUTHORS   Behnke,J., Schneppenheim,J., Koch-Nolte,F., Haag,F., Saftig,P. and
            Schroder,B.
  TITLE     Signal-peptide-peptidase-like 2a (SPPL2a) is targeted to
            lysosomes/late endosomes by a tyrosine motif in its C-terminal tail
  JOURNAL   FEBS Lett 585 (19), 2951-2957 (2011)
   PUBMED   21896273
  REMARK    GeneRIF: Data show that endogenous SPPL2a - in agreement with
            overexpression studies - is localised in membranes of
            lysosomes/late endosomes.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB041547.1, AL732330.14 and AI853709.1.
            
            On Sep 18, 2009 this sequence version replaced NM_023220.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB041547.1, SRR9219382.136889.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1727              AB041547.1         1-1727
            1728-5392           AL732330.14        199465-203129       c
            5393-5629           AI853709.1         19-255
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 61.76 cM"
     gene            1..5629
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /note="signal peptide peptidase like 2A"
                     /db_xref="GeneID:66552"
                     /db_xref="MGI:MGI:1913802"
     exon            1..289
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             224..1795
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /EC_number="3.4.23.-"
                     /note="IMP-3; protein SPPL2a; SPP-like 2A protein;
                     intramembrane protease 3; presenilin-like protein 2"
                     /codon_start=1
                     /product="signal peptide peptidase-like 2A precursor"
                     /protein_id="NP_075709.2"
                     /db_xref="CCDS:CCDS16690.1"
                     /db_xref="GeneID:66552"
                     /db_xref="MGI:MGI:1913802"
                     /translation="MGLLHSLHAPAAALLWSCLLGLAAAQEAILHASTNGVSSLSKDY
                     CMYYNNNWTRLPSSLENATSLSLMNLTGTALCHLSDIPPDGIRNKAVVVHWGPCHFLE
                     KARIAQEGGAAALLIANNSVLIPSSRNKSTFQNVTVLIAVITQKDFKDMKETLGDDIT
                     VKMYSPSWPNFDYTLVVIFVIAVFTVALGGYWSGLIELENMKSVEDAEDRETRKKKDD
                     YLTFSPLTVVVFVVICCIMIVLLYFFYRWLVYVMIAIFCIASSMSLYNCLSALIHRMP
                     CGQCTILCCGKNIKVSLIFLSGLCISVAVVWAVFRNEDRWAWILQDILGIAFCLNLIK
                     TMKLPNFMSCVILLGLLLIYDVFFVFITPFITKNGESIMVELAAGPFENAEKLPVVIR
                     VPKLMGYSVMSVCSVPVSVLGFGDIIVPGLLIAYCRRFDVQTGSSIYYISSTIAYAVG
                     MIITFVVLMVMKTGQPALLYLVPCTLITVSVVAWSRKEMKKFWKGSSYQVMDHLDYST
                     NEENPVTTDEQIVQQ"
     exon            290..409
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            410..592
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            593..682
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            683..816
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            817..968
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1065
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1167
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..1249
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1324
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1381
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1484
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1485..1559
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1720
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..5616
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5608..5613
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5616
                     /gene="Sppl2a"
                     /gene_synonym="2010106G01Rik; C130089K23Rik"
                     /note="major polyA site"
ORIGIN      
        1 aacccgaagc tccagtggct tggaatgggc ggcggccgca gttaggggga accgaacgag
       61 aacccaggat ttacgcagct aaaacggctc tgccaggagc tgacagtacc cacagagtcc
      121 cggcctagcg tttcgcggga gggaccggaa gaggaagtcg gggctgcacc ctagaggtcc
      181 gggtgccgcc agcgcagcct gagcctagct gcccgcccgt gggatggggc tgctgcactc
      241 gctgcacgct cccgcggctg ccctgctctg gagctgcctg ctcggactgg cagctgctca
      301 ggaagccatt ctgcatgcgt ctacaaatgg cgtgtcttca ctttccaagg actactgcat
      361 gtattataac aacaactgga cacgacttcc aagttcccta gaaaatgcaa cttctctgag
      421 cttgatgaat ctgacgggca cagcactgtg ccacctttct gatattcctc ctgatggcat
      481 aaggaacaag gcagtggtgg tgcactgggg accctgccat tttcttgaaa aagccagaat
      541 tgcacaggaa ggtggtgctg cagcattgct gattgccaac aacagtgtcc taattccttc
      601 ctcaaggaac aaatctacat ttcaaaatgt gactgtacta attgcagtta taacccaaaa
      661 agacttcaaa gacatgaaag agactcttgg agatgacatt accgtgaaaa tgtattcccc
      721 atcctggcct aactttgact atactctggt ggttattttt gtaattgctg tgttcactgt
      781 ggccttagga ggatactgga gtggacttat tgaattggaa aacatgaagt cagtggaaga
      841 cgcagaagac agagagacaa gaaaaaagaa ggacgattac ttaacattca gtcctctcac
      901 ggttgttgtg ttcgtggtca tctgctgtat aatgattgtc ttactgtatt tcttctacag
      961 gtggcttgtg tatgttatga tagcgatttt ctgcatagca tcgtcaatga gcctgtacaa
     1021 ctgtctttct gcattgattc ataggatgcc atgtggacag tgcacgattt tgtgttgtgg
     1081 caaaaacatt aaagtgagcc tgattttcct ttccgggctg tgcatctcag ttgctgttgt
     1141 gtgggctgta tttcgaaatg aggataggtg ggcttggatt ttacaggata tcttaggaat
     1201 tgctttctgt ctcaacttaa ttaaaacaat gaagttaccc aatttcatgt catgcgtgat
     1261 actgctaggc cttcttctca tctacgatgt gttctttgtc ttcatcacac ctttcatcac
     1321 aaagaatggt gagagcatca tggtggaact tgctgctggg cctttcgaga atgctgaaaa
     1381 gttgccagta gttatcaggg tgccaaaact gatgggttac tcagtcatga gtgtatgctc
     1441 cgtgcctgtt tcagtattgg gtttcggaga tatcattgta ccaggcctgt tgattgcata
     1501 ttgtagaaga ttcgatgttc agactggttc ttctatatac tatatttcat ccacaattgc
     1561 ctatgctgtt ggcatgatca ttacctttgt tgtcctgatg gtgatgaaaa cagggcagcc
     1621 tgctctcctc tacttggtac cttgtacact tattactgtc tcagtcgttg cttggagtcg
     1681 taaggaaatg aaaaagttct ggaaaggcag cagctatcag gtgatggacc acctggacta
     1741 ttcaacaaat gaagaaaatc cagtgacgac tgatgagcag attgtacaac agtaattacg
     1801 tggacttaca atgatgtgtt attgatttcc tacaaataca ttttggcctt tttttctttt
     1861 ttgctcttgg agacagaggc aggactggag ttcagtcctg cctctgtctc ccaaaggttt
     1921 agttaggact atagctggac aacactatgc ccagcaagtt ttaaatcaaa gtttgagttg
     1981 aaaattggga acatttacag cgatgtttgc aaatctgtat atttttatga gttggtaaaa
     2041 atcccatcat acatagtgaa aatgcatttt cttttaatta tgttaaaatt gtgccttcac
     2101 agtcagctta aaaacatagc gtttgtgtag tttccaaacg aattatatac attatatttt
     2161 tctaaaaaag aaaaaaaaaa gctaccctga ccttcctttg tacttttctt aatgaaatat
     2221 ttctccaaat gctaaggtta gatcatgtgt aagtgtcaaa gcaagtttct gagtatatta
     2281 aagatttatg taacttttaa cccctctaca gaaataaata cttaagactg caagaagaca
     2341 gttcatgtat atgggtgttt tgcctgtatg catctgtctg tacaccacat ttgtgcctgg
     2401 tacctggtgc ctggtgccaa tggagaccag aggagggtgt tggattccct gggactggag
     2461 ttacagatgg ttgtcagctg ccctttgtgt tctgggaatc aaacccaggt cctcagaaag
     2521 agcagcaagt gctcctaact gctgagccat ctcttcatcc ttgtcgttta agcttttaaa
     2581 aacgtgtaac cctttttaaa cctgagaacc taaattcttt catctgtgta cacatgctac
     2641 tagactgttt tttaggtcga aggaaacata gaagtagttg ttagaaggac accagaactg
     2701 aatctagttc ttatctagtt cctatggttt acttgaagat acagattaaa cttctgtatt
     2761 aaacttcagt ttgttttttt ctgatctttg aaatttgatt ccattaagta agatttcttc
     2821 ttttaaatgt ttttcttaac tgactagata gtatatgttt aacttattgt ttgtcttgac
     2881 tatcctgaca atacttatat catcttcacc ctgctttcca gtccttcttg tgtaagtgag
     2941 gtctggtaac cacaagtgtt ctgaagtcca tctcttttct cacctctaaa atgagtgagt
     3001 gctccactca tttaaccact ttgctagctt gcaacctgaa aaaaaaattt ccttctgcag
     3061 ggcgtggtgg cgcacgcctt tgatcccagc actcgggagg cagaggcagg cggatttctg
     3121 agtttggggc cagcctggtc tacagagtga gttccaggac agccagagct acacagagaa
     3181 accctgcctc aaaaacaaaa caaaacaaaa caaacaaaca aagaaacaaa tacacacaga
     3241 tatatttccg tctgattctt tgcaaaatct caggtgtttt tctagaaatt agaggtgaaa
     3301 ttatcttcaa gttttgtttt gtttttttaa aatttctcta agaaagtacc aggtgacaag
     3361 tcatcgcaga agcgcaggct tttgtgtttc tgtatcaaca ttgcacagga gacgtgagca
     3421 gtccagtcgt tcatgttaag gcaaagccag cttcttcaaa gtaaaacagg catactgaaa
     3481 aatgggttag acctttgata tttgaactaa agttcacatt aaatgctttt ttgtttaatt
     3541 gttaactttt gctgagtaga tgacaaagaa gcgggactgc ttcgttcttc taggtaccgg
     3601 atggggtctt ttgtttacag taaagctatc tggcaagaga taagtgccat ttacaattct
     3661 aactgtactt tcacatcaac tgtcaataaa atgcctgcca aaatacagct gttttagtaa
     3721 catctgcatt cccattcaag tcgcgatatt caaaagcaac agttacaatt ttatggagac
     3781 tattaaagaa aattattctt tttaaaacca cattgaaata agatgatatc tcaaaataat
     3841 ttggttttat taatttgtag ctaatatctt aatttcctaa tctaaacttt tgctccagaa
     3901 aaccatgaac acatctacaa atctgaattc caacttattg gaaacatttt ctattttacc
     3961 cagccatcat attgtcttct atatggtctg gagtgtatca gtgttagagt acttgcctac
     4021 catgttcaaa gtgccttccc cagcactgcc aatgaaacaa cttactaagt agctataaat
     4081 aacaaaagaa tttcctagag tctctctacc ctcactgcca atttcatgtt tacttaaaca
     4141 gccctcctag caacatagtt gaaaatacct ccatagagaa aataaaagac agaaatactt
     4201 tgtcttggaa atcaaaacta atagatacca gagttgaaaa tttgtctctg aaaatttgtc
     4261 atctcttcct ctcttgctaa cctaacacca atacttttta agaggtagat aagttggatt
     4321 tctttcccct taaacatgct tttaaaacaa aatatattgt atttattagt atttactaca
     4381 aaataaaata cttgctagaa agttccacac ttgtggctta tcaaatagaa atcactgatt
     4441 ttttttgttt gtttgttagt tttttttcaa gacagaggtc tctctgtgta gccctggctg
     4501 tcctggagct cactctgtag accagatggg cttctaatgc agagatctgc ctgcctctgc
     4561 ctcccgagtg ctgggaaaat attaatcata aatgcatttt tattttcttc tagcttgtgg
     4621 ctgactttaa ttcgcaatta cactttatac tagaatgtga ttcgatgccc ttttgaagga
     4681 aatctgtcct tccatatgct gctggcattc atgtatttta gttactgtgc atacaggttt
     4741 gggtagagtg gcattttata ataactccaa tgagctttct aagttccttt gctctgtcaa
     4801 tgtccacatt tctaaagcga tttttccgtc atggggcgct tatccagggc tgtgcacaca
     4861 ctgagcaagt gctgcgccca cctctgagca gtatgtccag ctcctccgtc tcggctctag
     4921 gtcaaagcag gattatttta cattggatga aaatatatct agtctgttaa actgattcgc
     4981 tagaactccg gtttgattcc tgtcagtgaa ctttcagagc tggcagttgg actcccgatg
     5041 ggtagactac actagttaaa gcagcctttg tagttttctg taaataagaa aaatagactg
     5101 gatcaactca aggatgtagt ggttgatcag ttcagcacgt cacgtggtgg gtttgactag
     5161 gcattacaaa tactataaac agcgttccta ttcctttgaa atatacataa aaaataccta
     5221 ccatatatct ggttggcctt caacttcccc cataggacaa gtgagtgtaa attcagtagc
     5281 tttttgtcca ctttcccccc taatacattg tactcaaatt agtaactttt taagaatgca
     5341 taattggatt tttacttctt agatgattgg tttgaatatg agattctttt tctaacactt
     5401 ttctatgtaa gttagtgtaa attaatattt ttataacact attagacttt ttatggaaca
     5461 tgatttaaga cttaaatttt tggagtatta atttagctgt aagttaagct ctcttcatca
     5521 tgatgtgcca attctgacaa gaaaaacaaa aacaacaaac ctttcagtgt attgaattta
     5581 ctgtgtgctc aataaatttt attttaaaat aaaaaaaaaa aaaaaaaaa
//
