LOCUS       NM_009263               1428 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus secreted phosphoprotein 1 (Spp1), transcript variant
            4, mRNA.
ACCESSION   NM_009263 XR_106288 XR_106289 XR_107716 XR_107717 XR_107718
VERSION     NM_009263.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Shigesawa,I., Nakamura,A., Yamauchi,Y., Kawata,S., Miyazaki,A.,
            Nomoto,H., Kameda,H., Terauchi,Y. and Atsumi,T.
  TITLE     Effects of glucokinase haploinsufficiency on the pancreatic
            beta-cell mass and function of long-term high-fat, high-sucrose
            diet-fed mice
  JOURNAL   J Diabetes Investig 15 (12), 1732-1742 (2024)
   PUBMED   39305123
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Datta,A., Li,X.Y. and Nagpaul,M.
  TITLE     Early expression of osteopontin glycoprotein on the ocular surface
            and in tear fluid contributes to ocular surface diseases in type 2
            diabetic mice
  JOURNAL   PLoS One 19 (10), e0313027 (2024)
   PUBMED   39480896
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1428)
  AUTHORS   Yang,W., Jin,M., Gu,Y., Zhao,X., Zhu,L., He,S., Wang,H., Ding,X.,
            Wang,B., Jiang,T., Xiao,Y., Zhou,G., Huang,J. and Zhang,Y.
  TITLE     Intracellular osteopontin potentiates the immunosuppressive
            activity of mesenchymal stromal cells
  JOURNAL   Stem Cell Res Ther 15 (1), 366 (2024)
   PUBMED   39407354
  REMARK    GeneRIF: Intracellular osteopontin potentiates the
            immunosuppressive activity of mesenchymal stromal cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1428)
  AUTHORS   Wang,Y., Zhang,W., Yang,Y., Qin,J., Wang,R., Wang,S., Fu,W.,
            Niu,Q., Wang,Y., Li,C., Li,H., Zhou,Y. and Liu,M.
  TITLE     Osteopontin deficiency promotes cartilaginous endplate degeneration
            by enhancing the NF-kappaB signaling to recruit macrophages and
            activate the NLRP3 inflammasome
  JOURNAL   Bone Res 12 (1), 53 (2024)
   PUBMED   39242551
  REMARK    GeneRIF: Osteopontin deficiency promotes cartilaginous endplate
            degeneration by enhancing the NF-kappaB signaling to recruit
            macrophages and activate the NLRP3 inflammasome.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1428)
  AUTHORS   Jansson,K.P., Kuluva,J., Zhang,S., Swanson,T., Zhang,Y.,
            Zimmerman,K.A., Fields,T.A., Wallace,D.P., Rowe,P.S. and
            Stubbs,J.R.
  TITLE     Osteopontin deletion attenuates cyst growth but exacerbates
            fibrosis in mice with cystic kidney disease
  JOURNAL   Physiol Rep 12 (17), e70038 (2024)
   PUBMED   39238069
  REMARK    GeneRIF: Osteopontin deletion attenuates cyst growth but
            exacerbates fibrosis in mice with cystic kidney disease.
REFERENCE   6  (bases 1 to 1428)
  AUTHORS   Worcester,E.M., Blumenthal,S.S., Beshensky,A.M. and Lewand,D.L.
  TITLE     The calcium oxalate crystal growth inhibitor protein produced by
            mouse kidney cortical cells in culture is osteopontin
  JOURNAL   J Bone Miner Res 7 (9), 1029-1036 (1992)
   PUBMED   1414495
REFERENCE   7  (bases 1 to 1428)
  AUTHORS   Waterhouse,P., Parhar,R.S., Guo,X., Lala,P.K. and Denhardt,D.T.
  TITLE     Regulated temporal and spatial expression of the calcium-binding
            proteins calcyclin and OPN (osteopontin) in mouse tissues during
            pregnancy
  JOURNAL   Mol Reprod Dev 32 (4), 315-323 (1992)
   PUBMED   1497879
REFERENCE   8  (bases 1 to 1428)
  AUTHORS   Kozak,C.A. and Stephenson,D.A.
  TITLE     Mouse chromosome 5
  JOURNAL   Mamm Genome 3 Spec No, S65-S80 (1992)
   PUBMED   1498444
  REMARK    Review article
REFERENCE   9  (bases 1 to 1428)
  AUTHORS   Craig,A.M. and Denhardt,D.T.
  TITLE     The murine gene encoding secreted phosphoprotein 1 (osteopontin):
            promoter structure, activity, and induction in vivo by estrogen and
            progesterone
  JOURNAL   Gene 100, 163-171 (1991)
   PUBMED   2055467
REFERENCE   10 (bases 1 to 1428)
  AUTHORS   Groves,M.G. and Osterman,J.V.
  TITLE     Host defenses in experimental scrub typhus: genetics of natural
            resistance to infection
  JOURNAL   Infect Immun 19 (2), 583-588 (1978)
   PUBMED   415980
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124106.11, AK160540.1 and AI882055.1.
            
            On Feb 18, 2011 this sequence version replaced NM_009263.2.
            
            Transcript Variant: This variant (4) differs in the 5' UTR, uses a
            downstream in-frame start codon and lacks an exon in the coding
            region, compared to variant 1. Variants 4 and 5 encode the same
            isoform (4), which is shorter than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057858.1, AK154908.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 AC124106.11        51606-51612         c
            8-1414              AK160540.1         2-1408
            1415-1428           AI882055.1         1-14                c
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 50.68 cM"
     gene            1..1428
                     /gene="Spp1"
                     /gene_synonym="2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;
                     OP; Opn; Opnl; Ric; Spp-1"
                     /note="secreted phosphoprotein 1"
                     /db_xref="GeneID:20750"
                     /db_xref="MGI:MGI:98389"
     exon            1..86
                     /gene="Spp1"
                     /gene_synonym="2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;
                     OP; Opn; Opnl; Ric; Spp-1"
                     /inference="alignment:Splign:2.1.0"
     exon            87..154
                     /gene="Spp1"
                     /gene_synonym="2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;
                     OP; Opn; Opnl; Ric; Spp-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..985
                     /gene="Spp1"
                     /gene_synonym="2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;
                     OP; Opn; Opnl; Ric; Spp-1"
                     /note="isoform 4 precursor is encoded by transcript
                     variant 4; minopontin; early T-lymphocyte activation 1
                     protein; calcium oxalate crystal growth inhibitor protein;
                     bone sialoprotein 1; osteopontin-like protein; 44 kDa bone
                     phosphoprotein"
                     /codon_start=1
                     /product="osteopontin isoform 4 precursor"
                     /protein_id="NP_033289.2"
                     /db_xref="CCDS:CCDS19486.1"
                     /db_xref="GeneID:20750"
                     /db_xref="MGI:MGI:98389"
                     /translation="MRLAVICFCLFGIASSLPVKVTDSGSSEEKLYSLHPDPIATWLV
                     PDPSQKQNLLAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAESED
                     SVDSDESDESHHSDESDETVTASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQ
                     VSDEQYPDATDEDLTSHMKSGESKESLDVIPVAQLLSMPSDQDNNGKGSHESSQLDEP
                     SLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKDKLVLDPKSKEDDR
                     YLKFRISHELESSSSEVN"
     exon            155..190
                     /gene="Spp1"
                     /gene_synonym="2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;
                     OP; Opn; Opnl; Ric; Spp-1"
                     /inference="alignment:Splign:2.1.0"
     exon            191..271
                     /gene="Spp1"
                     /gene_synonym="2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;
                     OP; Opn; Opnl; Ric; Spp-1"
                     /inference="alignment:Splign:2.1.0"
     exon            272..313
                     /gene="Spp1"
                     /gene_synonym="2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;
                     OP; Opn; Opnl; Ric; Spp-1"
                     /inference="alignment:Splign:2.1.0"
     exon            314..595
                     /gene="Spp1"
                     /gene_synonym="2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;
                     OP; Opn; Opnl; Ric; Spp-1"
                     /inference="alignment:Splign:2.1.0"
     exon            596..1420
                     /gene="Spp1"
                     /gene_synonym="2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;
                     OP; Opn; Opnl; Ric; Spp-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1395..1400
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spp1"
                     /gene_synonym="2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;
                     OP; Opn; Opnl; Ric; Spp-1"
                     /note="hexamer: AATAAA"
     polyA_site      1417
                     /gene="Spp1"
                     /gene_synonym="2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1;
                     OP; Opn; Opnl; Ric; Spp-1"
                     /note="major polyA site"
ORIGIN      
        1 agccaccagc agctcacact gaagaagcat ccttgcttgg gtttgcagtc ttctgcggca
       61 ggcattctcg gaggaaacca gccaaggact aactacgacc atgagattgg cagtgatttg
      121 cttttgcctg tttggcattg cctcctccct cccggtgaaa gtgactgatt ctggcagctc
      181 agaggagaag ctttacagcc tgcacccaga tcctatagcc acatggctgg tgcctgaccc
      241 atctcagaag cagaatctcc ttgcgccaca gaatgctgtg tcctctgaag aaaaggatga
      301 ctttaagcaa gaaactcttc caagcaattc caatgaaagc catgaccaca tggacgacga
      361 tgatgacgat gatgatgacg atggagacca tgcagagagc gaggattctg tggactcgga
      421 tgaatctgac gaatctcacc attcggatga gtctgatgag accgtcactg ctagtacaca
      481 agcagacact ttcactccaa tcgtccctac agtcgatgtc cccaacggcc gaggtgatag
      541 cttggcttat ggactgaggt caaagtctag gagtttccag gtttctgatg aacagtatcc
      601 tgatgccaca gatgaggacc tcacctctca catgaagagc ggtgagtcta aggagtccct
      661 cgatgtcatc cctgttgccc agcttctgag catgccctct gatcaggaca acaacggaaa
      721 gggcagccat gagtcaagtc agctggatga accaagtctg gaaacacaca gacttgagca
      781 ttccaaagag agccaggaga gtgccgatca gtcggatgtg atcgatagtc aagcaagttc
      841 caaagccagc ctggaacatc agagccacaa gtttcacagc cacaaggaca agctagtcct
      901 agaccctaag agtaaggaag atgataggta tctgaaattc cgaatttctc atgaattaga
      961 gagttcatct tctgaggtca actaaagaag aggcaaaaac acagttcctt actttgcatt
     1021 tagtaaaaac aagaaaaagt gttagtgagg gttaagcagg aatactaact gctcatttct
     1081 cagttcagtg gatatatgta tgtagagaaa gagaggtaat attttgggct cttagcttag
     1141 tctgttgttt catgcaaaca ccgttgtaac caaaagcttc tgcactttgc ttctgttctt
     1201 cctgtacaag aaatgcaaac ggccactgca ttttaatgat tgttattctt ttatgaataa
     1261 aatgtatgta gaaacaagca aatttactga aacaagcaga attaaaagag aaactgtaac
     1321 agtctatatc actataccct tttagtttta taattagcat atattttgtt gtgattattt
     1381 tttttgttgg tgtgaataaa tcttgtaacg aatgtaagga aaaaaaaa
//
