LOCUS       NM_172660               1838 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus SPOUT domain containing methyltransferase 1 (Spout1),
            mRNA.
ACCESSION   NM_172660 NM_001166622
VERSION     NM_172660.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Irvine,R.A., Adachi,N., Shibata,D.K., Cassell,G.D., Yu,K.,
            Karanjawala,Z.E., Hsieh,C.L. and Lieber,M.R.
  TITLE     Generation and characterization of endonuclease G null mice
  JOURNAL   Mol Cell Biol 25 (1), 294-302 (2005)
   PUBMED   15601850
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
REFERENCE   3  (bases 1 to 1838)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY289934.1, AK147162.1 and BY462424.1.
            
            On or before Sep 2, 2010 this sequence version replaced
            NM_001166622.1, NM_172660.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147162.1, SRR1660817.120330.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                BY289934.1         5-15
            12-1812             AK147162.1         2-1802
            1813-1838           BY462424.1         405-430
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 21.29 cM"
     gene            1..1838
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /note="SPOUT domain containing methyltransferase 1"
                     /db_xref="GeneID:227695"
                     /db_xref="MGI:MGI:106544"
     exon            1..70
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1192
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /note="centromere protein 32; kinetochore-associated
                     protein"
                     /codon_start=1
                     /product="putative methyltransferase C9orf114 homolog"
                     /protein_id="NP_766248.3"
                     /db_xref="CCDS:CCDS15873.2"
                     /db_xref="GeneID:227695"
                     /db_xref="MGI:MGI:106544"
                     /translation="MAERPRKRPCGPGEHGQRVEWRKWKQQKKEEKKKWKDLKIMKKL
                     ERQRAQEEEAKRQEEEEEAAAQRSNQGRPYTLSVALPGSILDNAQSPELRTYLAGQIA
                     RACTIFCVDEIVVFDEEGQDTKSVEGEFRGVGKKGQACVQLARILQYLECPQYLRKAF
                     FPKHQDLQFAGILNPLDSPHHMRQDEESEFREGVVVDRPTKAGHGSLVNCGMKKEVKI
                     DKKLDPGLRVTVRLNQQQLPECKTYKGTVVSSQDPRTKAGLYWGYTVRLASCLSAVFA
                     EAPFQDGYDLTIGTSERGSDVASAQLPSFRHALVVFGGLQGLEAAVDADPNLEVADPS
                     VLFDFYVNTCLSQGSRTIRTEEAILISLAALQPGLTQVGSRPASPLSGPRM"
     exon            71..116
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     exon            117..245
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     exon            246..405
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     exon            406..495
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     exon            496..545
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     exon            546..676
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     exon            677..749
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     exon            750..848
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     exon            849..951
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1099
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     exon            1100..1838
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1795..1800
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /note="hexamer: AATAAA"
     polyA_site      1814
                     /gene="Spout1"
                     /gene_synonym="9930028P05; CENP-32; D2Wsu81e"
                     /note="major polyA site"
ORIGIN      
        1 ggctaactgg caggcgcgcg gtgggcctgg aaacatggcg gagcgcccga ggaagcgtcc
       61 gtgtggtccg ggtgaacatg gccaaagggt tgaatggcga aaatggaaac agcagaaaaa
      121 agaggagaaa aagaagtgga aggacctcaa gataatgaag aaactggagc ggcagcgtgc
      181 ccaagaggaa gaggctaaac gccaggaaga ggaggaagag gcggcggccc agaggagcaa
      241 ccaaggacgg ccttacactc tgagtgtggc cctgccaggc tccatcctgg acaatgccca
      301 gtcacctgag ctccgcacct acctggctgg ccagatcgcc agagcctgca ccatcttctg
      361 tgtggatgag attgtggtgt tcgatgagga gggccaagat accaagagtg tggaaggaga
      421 attcagggga gttggcaaga aagggcaggc atgtgtccag ctggcccgca tcctacagta
      481 cctggagtgt ccacagtacc tgagaaaggc gttcttcccc aagcaccaag atctgcagtt
      541 tgcaggaatc ctcaacccct tggacagccc tcaccacatg cgtcaggacg aagagtctga
      601 gtttcgagaa ggcgtcgtgg tggaccggcc caccaaggca ggccatggct ctttggtcaa
      661 ctgtggaatg aagaaggagg tgaagattga caagaaactg gaccctggac ttcgggtgac
      721 cgtgcgactg aaccagcagc agctcccaga atgcaagaca tacaaaggaa cagttgtgtc
      781 atcacaggac ccccgcacca aagctggtct ctactggggc tacactgtcc ggctggcctc
      841 ttgcctcagc gctgtgtttg ctgaggctcc cttccaggat gggtacgacc tgaccattgg
      901 gacatcagaa cgtggctccg acgtggcctc tgcccagctg cccagcttca ggcatgccct
      961 tgtggtgttt ggaggcctcc aagggctaga agctgcagtg gatgctgacc cgaaccttga
     1021 agtggctgac cccagtgtcc tcttcgactt ctatgtcaac acatgcctta gccagggcag
     1081 ccgtaccatc cgcaccgagg aggccatact catctcgctg gctgctctgc agcctggtct
     1141 cacccaggtg ggatcgaggc ccgcctcccc gctttctgga cccaggatgt agaggaagca
     1201 gcaggacagg aggatcatgg gagtagagat ggtcaagtct tctctccaaa aataactgcc
     1261 tttaatgtga gtcagcccat ctccccaggg gctccagtga gtcctccacc atcacttgct
     1321 gccagcagtg atagccttga ggtttccagc tcgagccaga atattgggca cgaagagcaa
     1381 tcccgaggcc cgctcgatgc tctcgatggg caccaggaac cgctccagag ggatggtctc
     1441 atccacgggg gcattgggca tcacgtagga acgtagctcg atctgcccac cggctgcctc
     1501 caggatcagc accttgaaga agtgtgtggg cactgccacg tggttcttcc caataacctg
     1561 gtacttcaca taggacttcc catcagcctc ggtcctgtgg atagacccgg gcgcatggcc
     1621 aagaggcctg gttacataca aggtcacctc ttccaggaag ccttcctaac ttccaacaag
     1681 caagaagttt gtgctgggcc tggtgcctcc ttgggtctgg gaactatgta aggtggggac
     1741 caatgcttaa cctctgcttt tctgcccaat actgcttcag acagaatggg aggcaataaa
     1801 cgcagctaga ggaatgagct ctctagaccc tgatgctc
//
