LOCUS       NM_023689               3226 bp    mRNA    linear   ROD 06-AUG-2023
DEFINITION  Mus musculus sparc/osteonectin, cwcv and kazal-like domains
            proteoglycan 3 (Spock3), transcript variant 1, mRNA.
ACCESSION   NM_023689
VERSION     NM_023689.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Yamamoto A, Uchiyama K, Nara T, Nishimura N, Hayasaka M, Hanaoka K
            and Yamamoto T.
  TITLE     Structural abnormalities of corpus callosum and cortical axonal
            tracts accompanied by decreased anxiety-like behavior and lowered
            sociability in spock3- mutant mice
  JOURNAL   Dev Neurosci 36 (5), 381-395 (2014)
   PUBMED   25138526
  REMARK    GeneRIF: these results demonstrate that Spock3 plays an important
            role in the formation or maintenance of major neuronal structures
            in the brain.
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Hartmann U, Hulsmann H, Seul J, Roll S, Midani H, Breloy I, Hechler
            D, Muller R and Paulsson M.
  TITLE     Testican-3: a brain-specific proteoglycan member of the
            BM-40/SPARC/osteonectin family
  JOURNAL   J Neurochem 125 (3), 399-409 (2013)
   PUBMED   23418755
  REMARK    GeneRIF: The expression of testican-3 in adult mice is restricted
            to the brain and located diffusely within the extracellular matrix.
REFERENCE   3  (bases 1 to 3226)
  AUTHORS   Manabe R, Tsutsui K, Yamada T, Kimura M, Nakano I, Shimono C,
            Sanzen N, Furutani Y, Fukuda T, Oguri Y, Shimamoto K, Kiyozumi D,
            Sato Y, Sado Y, Senoo H, Yamashina S, Fukuda S, Kawai J, Sugiura N,
            Kimata K, Hayashizaki Y and Sekiguchi K.
  TITLE     Transcriptome-based systematic identification of extracellular
            matrix proteins
  JOURNAL   Proc Natl Acad Sci U S A 105 (35), 12849-12854 (2008)
   PUBMED   18757743
REFERENCE   4  (bases 1 to 3226)
  AUTHORS   Mozhui K, Hamre KM, Holmes A, Lu L and Williams RW.
  TITLE     Genetic and structural analysis of the basolateral amygdala complex
            in BXD recombinant inbred mice
  JOURNAL   Behav Genet 37 (1), 223-243 (2007)
   PUBMED   17131200
REFERENCE   5  (bases 1 to 3226)
  AUTHORS   Desai J, Shannon ME, Johnson MD, Ruff DW, Hughes LA, Kerley MK,
            Carpenter DA, Johnson DK, Rinchik EM and Culiat CT.
  TITLE     Nell1-deficient mice have reduced expression of extracellular
            matrix proteins causing cranial and vertebral defects
  JOURNAL   Hum Mol Genet 15 (8), 1329-1341 (2006)
   PUBMED   16537572
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122404.4, AK220280.1 and BC017601.1.
            
            On Nov 22, 2011 this sequence version replaced NM_023689.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1). Variants 1 and 2
            both encode the same isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC017601.1, AK220280.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AC122404.4         54080-54095
            17-2966             AK220280.1         1-2950
            2967-3226           BC017601.1         2866-3125
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 31.97 cM"
     gene            1..3226
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /note="sparc/osteonectin, cwcv and kazal-like domains
                     proteoglycan 3"
                     /db_xref="GeneID:72902"
                     /db_xref="MGI:MGI:1920152"
     exon            1..184
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
     CDS             185..1495
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; testican-3"
                     /codon_start=1
                     /product="testican-3 isoform 1 precursor"
                     /protein_id="NP_076178.1"
                     /db_xref="CCDS:CCDS22326.1"
                     /db_xref="GeneID:72902"
                     /db_xref="MGI:MGI:1920152"
                     /translation="MLKVSALLCVCAAAWCSQTLAAAAAVAVAGGRSDGGNFLDEKQW
                     LTTISQYDKEVGQWNKFRDEVEDDYFRTWNPGKPFDQALDPAKDPCLKTKCSRHKVCI
                     TQDAQTALCISHRRLTHSMKEVGGSHKQWRGLPSSTCKPCPIAYASPVCGSDGHSYSS
                     QCKLEYQACVLGKQISIKCEGRCPCPSDKSMNIGRNVKRACSDLEFREVANRLRDWFK
                     ALHESGSQNKKTKALLRPERSRFDTSILPICKDSLGWMFNRLDTNYDLLLDQSELGSI
                     YLDKNEQCTKAFFNSCDTYKDSLISNNEWCYCFQRQQDPPCHTELSNIQKRQGIKKLL
                     GQYIPLCDEDGYYKPTQCHGSVGQCWCVDRYGNEVVGSRINGVADCAIDFEISGDFAS
                     GDFREWTDDEGEEDDIMNDKDDIEDDDEDEGDDDDDGDVHDGYI"
     exon            185..373
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
     exon            374..382
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
     exon            383..428
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
     exon            429..543
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
     exon            544..667
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
     exon            668..782
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
     exon            783..902
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1124
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1187
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1325
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..3212
                     /gene="Spock3"
                     /gene_synonym="2900045C01Rik; mKIAA4039"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgaagcgagg ggagctggcg agggataggc gcggggaggg tgggatgggg ggagcgccgg
       61 gcgcagggag ctgagtggac ggctgagacg gcggcgcgtg cagcagctcc agcaagaagc
      121 gagttgccag agccgggctg cttttccatc aggagctgcg agctccgtgc ccgctctcaa
      181 caagatgctc aaggtgtcag ccttattgtg tgtgtgtgca gcggcttggt gcagccagac
      241 tcttgcagct gccgcggcgg tggctgtggc cggggggcgg tcggacggcg gtaattttct
      301 ggacgaaaaa caatggctca cgacaatctc tcagtatgac aaggaagtcg gacagtggaa
      361 caaattccga gacgaagtag aggatgatta tttccgcact tggaatccag gaaaaccatt
      421 tgatcaagct ttagatcctg ctaaggaccc atgcttaaag acgaaatgca gccgccacaa
      481 agtttgcatt acgcaggatg ctcagactgc actgtgtatc agtcaccgga ggctcacaca
      541 cagtatgaaa gaagtaggag gaagccataa gcaatggaga ggtctcccgt catctacctg
      601 caagccatgc cctatcgcct atgccagccc agtctgtggt tctgatgggc attcctactc
      661 ttcacagtgc aaactagaat atcaggcatg tgtcttagga aaacagatct ctatcaaatg
      721 tgaaggacgt tgcccatgtc cctccgataa gtccatgaac ataggcagaa atgttaaaag
      781 agcctgcagt gacctggaat tcagagaagt ggcaaacaga ctgcgagact ggttcaaggc
      841 ccttcatgag agtggaagcc agaacaagaa gacaaaagca ttgctgaggc ctgagagaag
      901 cagatttgat accagtattt tgccaatttg caaggattca cttggctgga tgtttaatag
      961 acttgataca aactatgacc tgctattgga ccagtcagag cttgggagca tataccttga
     1021 taagaatgag cagtgcacca aggcgttctt caattcttgt gacacttaca aggacagttt
     1081 gatatctaat aatgaatggt gctactgttt ccagagacag caagacccac cttgccacac
     1141 ggagctcagt aacattcaga agcgacaggg gataaagaag ctcctaggac agtacattcc
     1201 actctgtgat gaagatggtt actacaaacc aacacaatgc catggcagtg tagggcagtg
     1261 ctggtgtgtt gacagatatg gaaatgaagt tgttggatca agaataaatg gtgttgcaga
     1321 ttgtgctata gactttgaga tatctggaga tttcgcaagt ggagattttc gtgaatggac
     1381 tgatgatgaa ggtgaagaag atgacattat gaatgataag gatgatattg aagatgatga
     1441 tgaagatgaa ggggatgatg atgacgatgg ggatgtccat gatggatata tttgatcagt
     1501 aacagctaat gtcaataaat tctacatttt taatatttac aaaataatag catattgaga
     1561 atttccttct tatccctaaa caaagtggtt ctaaacctga catatatttt gtataattat
     1621 ttcaaatatt gaagctaaaa tcatagaact tgatgttaaa ttagaattgt ttaatttcag
     1681 ttttcatatg ccttagaaaa aaagaaaaca catatgggtt gcagcctgaa tatagaaagt
     1741 tgtaaagatt actatagcaa aaatttcata agaaaatact atgtaatttt tccacaaaca
     1801 aaataacagt gaatgtgtgc ttgggatcat gtgtgttgct tttatgacag attgtataag
     1861 ttaaatttaa aataggtaac taatgacctg aaccacaata atttcatcga ttaactcatg
     1921 atgtttactt caattcaaat ttggcatctt cccaagactt atagttggta ttttaagaaa
     1981 aacaactcat ggtaagagaa agtggtaatc ttttcaatcg tgaagttata ctgggtatga
     2041 ttaaatatta tatgcaatgc aaactatgga aaactttggg ttctgaaagt taaaaactta
     2101 agatttttct ttctactttt taatttaaat agcattgcat aacatgtgga tttttaaaaa
     2161 tattccttga caacctcatt tctcagtttc tctctctttc tgagtagtaa tcatgtatag
     2221 aatttcaatt agaaggagaa acagacttat ttcaacccct ttttctataa aatataattt
     2281 atttgtccca agatcaattt tgaaaatatg aactctcagt tttcatttag tataaaattt
     2341 tgcatttgat cttggaatca tgccattttt gttagtggta ttgaaattag agaccatgtt
     2401 gtatggtgcc ttgcaaaaat agaaatagaa agggttagca tgtatatcag tatagaagac
     2461 taggcaatgt gtaagcacac ataattaaca aattattgct atttaaatac tgctagaatg
     2521 aagaaaatgg aatatgtttc tttctttttt tctattgtta cataatccca tatggacttg
     2581 ttcataatta ttgtgaagag tagcatttaa gattacctgt aacgaaagtt actttgttca
     2641 ctatacatca gttagcatgt taattaattg tgtagacatt ttgacatacc accacctttg
     2701 ggtatatcac accactagtt ttgtgctcat aatagaagtg ggaaaacctg ttgaatttta
     2761 cacattggta tctttcactt caacagtgaa tttacagatt tccaggaata tcattcataa
     2821 gaacacaaat ttggtgaact cttgccattt aatttatata cagtaaataa aaactttaat
     2881 gttgaactta cataatattt aagctataat gcattttctt ttttttactg tttatgtata
     2941 gtttacaaat aaagaatctt gtaaccaaag taaacagttc cttaatgatt ttcttgggta
     3001 tacataatag catgaagagt ttttaattta ttccttaatt ctgcaaaata acataccaca
     3061 acagagccaa taagtttttc tcagtttatt tttaagtttt taaacctgat ttgttagtgt
     3121 cttgggtaga atatatttat ctttgtttgt tggtttattt taatctgttt atttgtcaaa
     3181 atattgagaa ataaatactt ttctttccaa aaaaaaaaaa aaaaaa
//
