LOCUS       NM_052994               3931 bp    mRNA    linear   ROD 01-JUL-2024
DEFINITION  Mus musculus sparc/osteonectin, cwcv and kazal-like domains
            proteoglycan 2 (Spock2), mRNA.
ACCESSION   NM_052994
VERSION     NM_052994.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Kang,J.Y., Cho,H., Gil,M., Lee,H., Park,S. and Kim,K.E.
  TITLE     The novel prognostic marker SPOCK2 regulates tumour progression in
            melanoma
  JOURNAL   Exp Dermatol 33 (6), e15092 (2024)
   PUBMED   38888196
  REMARK    GeneRIF: The novel prognostic marker SPOCK2 regulates tumour
            progression in melanoma.
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Hadchouel,A., Franco-Montoya,M.L., Guerin,S., Do Cruzeiro,M.,
            Lhuillier,M., Ribeiro Baptista,B., Boyer,L., Lanone,S. and
            Delacourt,C.
  TITLE     Overexpression of Spock2 in mice leads to altered lung alveolar
            development and worsens lesions induced by hyperoxia
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 319 (1), L71-L81 (2020)
   PUBMED   32374670
REFERENCE   3  (bases 1 to 3931)
  AUTHORS   Magella,B., Adam,M., Potter,A.S., Venkatasubramanian,M., Chetal,K.,
            Hay,S.B., Salomonis,N. and Potter,S.S.
  TITLE     Cross-platform single cell analysis of kidney development shows
            stromal cells express Gdnf
  JOURNAL   Dev Biol 434 (1), 36-47 (2018)
   PUBMED   29183737
REFERENCE   4  (bases 1 to 3931)
  AUTHORS   Naba,A., Clauser,K.R., Mani,D.R., Carr,S.A. and Hynes,R.O.
  TITLE     Quantitative proteomic profiling of the extracellular matrix of
            pancreatic islets during the angiogenic switch and insulinoma
            progression
  JOURNAL   Sci Rep 7, 40495 (2017)
   PUBMED   28071719
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3931)
  AUTHORS   Yamamoto,A., Uchiyama,K., Nara,T., Nishimura,N., Hayasaka,M.,
            Hanaoka,K. and Yamamoto,T.
  TITLE     Structural abnormalities of corpus callosum and cortical axonal
            tracts accompanied by decreased anxiety-like behavior and lowered
            sociability in spock3- mutant mice
  JOURNAL   Dev Neurosci 36 (5), 381-395 (2014)
   PUBMED   25138526
REFERENCE   6  (bases 1 to 3931)
  AUTHORS   Schnepp,A., Komp Lindgren,P., Hulsmann,H., Kroger,S., Paulsson,M.
            and Hartmann,U.
  TITLE     Mouse testican-2. Expression, glycosylation, and effects on neurite
            outgrowth
  JOURNAL   J Biol Chem 280 (12), 11274-11280 (2005)
   PUBMED   15657052
  REMARK    GeneRIF: Both the proteoglycan and core protein forms of testican-2
            inhibited neurite extension from cultured primary cerebellar
            neurons and may play regulatory roles in the development of the
            central nervous system.
REFERENCE   7  (bases 1 to 3931)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   8  (bases 1 to 3931)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 3931)
  AUTHORS   Vannahme,C., Schubel,S., Herud,M., Gosling,S., Hulsmann,H.,
            Paulsson,M., Hartmann,U. and Maurer,P.
  TITLE     Molecular cloning of testican-2: defining a novel calcium-binding
            proteoglycan family expressed in brain
  JOURNAL   J Neurochem 73 (1), 12-20 (1999)
   PUBMED   10386950
REFERENCE   10 (bases 1 to 3931)
  AUTHORS   Kambouris,M., Triarhou,L.C., Dlouhy,S.R., Sangameswaran,L., Luo,F.,
            Ghetti,B. and Hodes,M.E.
  TITLE     Novel cDNA clones obtained by antibody screening of a mouse
            cerebellar cDNA expression library
  JOURNAL   Brain Res Mol Brain Res 25 (3-4), 183-191 (1994)
   PUBMED   7808216
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC057324.1.
            
            On Dec 20, 2003 this sequence version replaced NM_052994.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC057324.1, AK122233.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 29.69 cM"
     gene            1..3931
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /note="sparc/osteonectin, cwcv and kazal-like domains
                     proteoglycan 2"
                     /db_xref="GeneID:94214"
                     /db_xref="MGI:MGI:1891351"
     exon            1..400
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..1483
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /note="granule cell antiserum positive 26; testican-2"
                     /codon_start=1
                     /product="testican-2 precursor"
                     /protein_id="NP_443720.1"
                     /db_xref="CCDS:CCDS35910.1"
                     /db_xref="GeneID:94214"
                     /db_xref="MGI:MGI:1891351"
                     /translation="MRAPGSGRLALPLLLLAVVALAEGDAKGLKEGETPGNFMEDEQW
                     LSSISQYSGKIKHWNRFRDEVEDDYIKSWEDNQQGDEALDTTKDPCQKVKCSRHKVCV
                     AQGYQRAMCISRKKLEHRIKQPSLKLHGGKDSVCKPCHMAQLASVCGSDGHTYSSVCK
                     LEQQACLSSKQLAVRCEGPCPCPTEQSTASTTDSKSETCTGQDLADLGDRLRDWFQLL
                     RENSKQNGSANSATNPAGLDKSLGASCKDSIGWMFSKLDTSGDLFLDQTELAAINLDK
                     YEVCIRPFFNSCDTYKDGRVSTAEWCFCFWREKPPCLAELERTQIQEAAKKKPGVFIP
                     SCDEDGYYRKMQCDQSRGDCWCVDQLGLELTGTRMHGTPDCDDIVGFSGDFGSGVGWE
                     DEEEKETEEAGEEAEEEEGEAGEADDGGYIW"
     exon            401..409
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /inference="alignment:Splign:2.1.0"
     exon            410..455
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /inference="alignment:Splign:2.1.0"
     exon            456..570
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /inference="alignment:Splign:2.1.0"
     exon            571..685
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /inference="alignment:Splign:2.1.0"
     exon            686..800
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /inference="alignment:Splign:2.1.0"
     exon            801..917
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1136
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..1199
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1337
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..3916
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2174
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /note="major polyA site"
     regulatory      3457..3462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /note="hexamer: CATAAA"
     polyA_site      3484
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /note="major polyA site"
     regulatory      3894..3899
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /note="hexamer: AGTAAA"
     polyA_site      3916
                     /gene="Spock2"
                     /gene_synonym="Gcap26; mKIAA0275"
                     /note="major polyA site"
ORIGIN      
        1 cctctccgcg tccagcgctg ggctttttca gacaagtgca tctgctaacc aggtcacatt
       61 tcagccacga cccaccctcc gtcagtcact ggagtcagac agcaggagga gggctgagga
      121 ggaccgcagc gcttcgccag tggcgcggtg gagggaagga catcaaatcc tgctgaagtc
      181 cagacccccc caggtccaag cccagaccac gatgcgcgct ccaggctccg ggcggctggc
      241 tctgccgctg ctgctcctcg ccgtggtcgc cttggctgaa ggcgacgcca aggggctcaa
      301 ggagggcgag acccccggca atttcatgga ggacgagcaa tggctgtcgt ccatctcgca
      361 gtacagcggc aagattaagc actggaaccg cttccgagac gaggtagaag atgattacat
      421 caagagctgg gaagacaatc agcaaggaga tgaagctctg gataccacca aggacccctg
      481 ccagaaggtg aagtgcagtc gccacaaggt gtgtgttgct caaggctacc agcgcgccat
      541 gtgcatcagc cgcaagaagt tggagcacag gatcaagcag ccttctctga aactccacgg
      601 aggcaaagac tccgtctgca agccctgtca tatggcccag ctcgcttcag tctgtggctc
      661 cgacggccat acctacagct ctgtgtgtaa gctggagcag caggcatgcc taagcagcaa
      721 gcagctggcg gtgagatgtg agggcccctg tccctgtccc acggagcagt ctactgcgtc
      781 taccaccgac agcaagtcag agacctgcac agggcaggac ctggccgacc tgggggatag
      841 gctccgtgac tggttccagc tccttcgtga gaactccaag cagaacggct cagccaacag
      901 tgcaactaac cccgcagggt tggacaagag cctgggggcc agttgcaagg actccatcgg
      961 ttggatgttc tctaagctgg acactagtgg agacctcttc ctggaccaga cagaactggc
     1021 tgccatcaac ctggacaaat acgaggtctg catccgcccc ttcttcaact cctgtgatac
     1081 ctacaaagat ggccgcgtct ctactgctga gtggtgcttc tgtttctgga gggagaagcc
     1141 cccctgcctg gcagagctgg agcgcacaca gatccaggag gcagccaaga agaagccagg
     1201 tgtcttcatc ccgagctgtg atgaagatgg ctactaccgg aagatgcagt gtgaccagag
     1261 cagaggtgac tgctggtgtg tggaccagct gggcctggag ctgaccggca cacgcatgca
     1321 tgggacccct gactgtgatg acatcgtggg tttctccggg gactttggaa gcggcgttgg
     1381 ctgggaggat gaggaagaaa aggagacaga agaggcaggg gaggaggccg aggaggagga
     1441 gggtgaggcg ggtgaggcag atgatggagg ctacatctgg tagatagccc tcgacggcct
     1501 cggcaggcca gggtgccgac ggccagaaag acaggccaga agagacctgg acaacagcag
     1561 gcaacttagc aaatggatcc ggaagtcagt gcggaggacc ctggctccag tgggggagga
     1621 ctggggagtg tggcttgtgt gtatgtgtga tatgagatgt gtgtgtatga tgtgagatgt
     1681 gagtgtgtga tgtgagatgt gtgtatgtga gatgtgtgtg tgtgtgtgtg tgtgtgtgat
     1741 gtgagtcacc caaaggtccc tatgttattg attttcaaat gcacactttc acacacaagg
     1801 atacactaac agaccaaaat caatgagatg tccctgatcc tgcccctcag cccaacacct
     1861 ggaatccctg ccctgcctaa tcagccctac cccatcctgc ctttcttcct tcctcttctg
     1921 aggccaggga gagggaggga gggcaggcag ctgaaagctg agcgtcctcc ccaaattggg
     1981 ctgatttgat atgaaagccc caccacggtc ttgtggtgat gaggcaggac ctgtacacac
     2041 agcaaggctt actatccgtc tcctgacatc cgaagagctc gtcgcaggtg tgtctaatgg
     2101 cccttgggct gcacgtggtt aagcatagct gtctctgtgg cccaacacaa agaggtaaac
     2161 ttacttaaaa cacaggggtt ttgttgctgt ttgttttttg ttagtttgtt tgttgataat
     2221 tggattgagg agttaatcct ctgtcacaat gtcaaaagca tggacatccc tgatagatta
     2281 tctctcaacc tagtcccgag taaccagaga tagctcagca gaagaggcag aaatcatgag
     2341 acagaggaca atgggtccct tgatttcagg cctcagatta acccagtcag cacctgtggc
     2401 ctcatggggc tctgggctgc tttcttggcg aatggggact gtcatcatat agctgagatt
     2461 tggagtagcc tgctatgagg gcttgcagtt gcactctctg ttccctggcg agggtccccc
     2521 ttgtcccaag gagcagagga ccagcaagca gcccgtgtct gctcaccttc tccctgaccc
     2581 tctaggaaat ggttccctct tgccccgacc aatagaaggg cgtgtccccg agacccttgt
     2641 gaggaaccac ggggagctgt gacttcctgt gcccagaagg tcctggagga gaggccccaa
     2701 agccttagtc ctctcagcca tgactttcct ctgtctcaag ggtccccaag atggccctag
     2761 aagaaccatg ccccagactc tatttaaagc cctatagcat atatgaatag agaacggtgt
     2821 atctatcaga ggggaccaca ctgagcagct aggccctgat gccatgcctc agcacgccct
     2881 cagacctcca ggccgggtgg aaggggcttt tacccagatg tcctgtgggg ttttgtgtgt
     2941 ctattgctcc ccacttcccc ctctcaaagg aaatttcctt gatatatttg atgaagaatt
     3001 ccttacctgg gcctctgata ccctttggta cgcgtacgtg tgtgtggcgc gtatacgtgt
     3061 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtga gagagagaga gagagagaga
     3121 gagagagaga gagagaaaga gagagagaat gagagagaat gagagagaga atacccgagg
     3181 gccaccctga tgcccaggaa gagtgagggc agccccctgt gtggtggaag aggctgggca
     3241 gctagctact ggggaccttg tgctgaggct tccaggacaa agccccagtc tggctctccc
     3301 ccagcctctc aggggcactg gctggtcaca tccactgagt attgatcggt tggactttag
     3361 ggaagagcct cctccttccc attcctttct gccaccctgg aatggccacc ttaatcgtca
     3421 tctcctcggg gggaatacgg aaatgtcacc aagctccata aagtgcttct cattccacga
     3481 cacagttctg actctccggg tgactgtcac ctgctcccat cctcttcttc ctccctccct
     3541 tcctcagacc tctgcaacca tcctctggtc cctggctcag tcacatcagc cctctgcatt
     3601 ctagaagcag ccatcccgcc cccctctctc ctccccctcc tccccttcct cctccctcca
     3661 gctggagtta cttgaaactt cctgctctcg ctaggatgga gtctgattct ttctctgccc
     3721 cagctggatt tccagaacat ccccgggtag ctgtgaagga ttttaggctc tctggggtgg
     3781 ggagggggtg gtgtagagga agacgcatcc ccttctttca ccctgccccg ccctgccccg
     3841 ctggggagct atgctctgtg tgtccctgga tcttctggtt ttgtatctga tgtagtaaac
     3901 atgttttagt aagaaaaaaa aaaaaaaaaa a
//
