LOCUS       NM_009262               4674 bp    mRNA    linear   ROD 04-OCT-2024
DEFINITION  Mus musculus sparc/osteonectin, cwcv and kazal-like domains
            proteoglycan 1 (Spock1), transcript variant 1, mRNA.
ACCESSION   NM_009262
VERSION     NM_009262.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   O'Brown,N.M., Patel,N.B., Hartmann,U., Klein,A.M., Gu,C. and
            Megason,S.G.
  TITLE     The secreted neuronal signal Spock1 promotes blood-brain barrier
            development
  JOURNAL   Dev Cell 58 (17), 1534-1547 (2023)
   PUBMED   37437574
  REMARK    GeneRIF: The secreted neuronal signal Spock1 promotes blood-brain
            barrier development.
REFERENCE   3  (bases 1 to 4674)
  AUTHORS   Xie,Z., Ma,X.H., Bai,Q.F., Tang,J., Sun,J.H., Jiang,F., Guo,W.,
            Wang,C.M., Yang,R., Wen,Y.C., Wang,F.Y., Chen,Y.X., Zhang,H.,
            He,D.Z., Kelley,M.W., Yang,S. and Zhang,W.J.
  TITLE     ZBTB20 is essential for cochlear maturation and hearing in mice
  JOURNAL   Proc Natl Acad Sci U S A 120 (24), e2220867120 (2023)
   PUBMED   37279265
REFERENCE   4  (bases 1 to 4674)
  AUTHORS   Ebeid,M., Barnas,K., Zhang,H., Yaghmour,A., Noreikaite,G. and
            Bjork,B.C.
  TITLE     PRDM16 expression and function in mammalian cochlear development
  JOURNAL   Dev Dyn 251 (10), 1666-1683 (2022)
   PUBMED   35451126
REFERENCE   5  (bases 1 to 4674)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4674)
  AUTHORS   Charbonnier,F., Chanoine,C., Cifuentes-Diaz,C., Gallien,C.L.,
            Rieger,F., Alliel,P.M. and Perin,J.P.
  TITLE     Expression of the proteoglycan SPOCK during mouse embryo
            development
  JOURNAL   Mech Dev 90 (2), 317-321 (2000)
   PUBMED   10640720
REFERENCE   7  (bases 1 to 4674)
  AUTHORS   Ringuette,M., Rogers,I., Varmuza,S., Rush,S. and Brown,I.R.
  TITLE     Expression of SC1 is associated with the migration of myotomes
            along the dermomyotome during somitogenesis in early mouse embryos
  JOURNAL   Dev Genes Evol 208 (7), 403-406 (1998)
   PUBMED   9732554
REFERENCE   8  (bases 1 to 4674)
  AUTHORS   Sommer,B., Bickel,M., Hofstetter,W. and Wetterwald,A.
  TITLE     Expression of matrix proteins during the development of mineralized
            tissues
  JOURNAL   Bone 19 (4), 371-380 (1996)
   PUBMED   8894143
REFERENCE   9  (bases 1 to 4674)
  AUTHORS   Kirschbaum,T., Jaenichen,R. and Zachau,H.G.
  TITLE     The mouse immunoglobulin kappa locus contains about 140 variable
            gene segments
  JOURNAL   Eur J Immunol 26 (7), 1613-1620 (1996)
   PUBMED   8766569
REFERENCE   10 (bases 1 to 4674)
  AUTHORS   Bonnet,F., Perin,J.P., Charbonnier,F., Camuzat,A., Roussel,G.,
            Nussbaum,J.L. and Alliel,P.M.
  TITLE     Structure and cellular distribution of mouse brain testican.
            Association with the postsynaptic area of hippocampus pyramidal
            cells
  JOURNAL   J Biol Chem 271 (8), 4373-4380 (1996)
   PUBMED   8626787
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY241184.1, BC075625.1 and AC154748.2.
            
            On Oct 24, 2009 this sequence version replaced NM_009262.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC075625.1, X92864.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                BY241184.1         2-58
            58-2873             BC075625.1         1-2816
            2874-4674           AC154748.2         117202-119002       c
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 30.5 cM"
     gene            1..4674
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /note="sparc/osteonectin, cwcv and kazal-like domains
                     proteoglycan 1"
                     /db_xref="GeneID:20745"
                     /db_xref="MGI:MGI:105371"
     exon            1..141
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     exon            142..327
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     exon            328..336
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     exon            337..382
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     exon            383..497
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     exon            498..624
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     exon            625..739
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     exon            740..856
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     exon            857..1078
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1141
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1279
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..4674
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4650..4655
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /note="hexamer: AATAAA"
     polyA_site      4671
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /note="major polyA site"
ORIGIN      
        1 agagcgctgc gggctcacaa agcggccgga cgcgcggcgg cggcaggtgc gcaagggaac
       61 gagccgggca tcagccttcc cagccaccgc ggtacaggaa ccccagcaac tcgggcgccg
      121 ggactcgggc gggctcccaa gatgccagcg atcgcggtgc tcgcggcggc cgccgcggcg
      181 tggtgcttcc tccaagtgga cagccggcac ctggacgcgc tggccggtgg cgcggccctc
      241 aacaacgcca atttcctaga caatgaccag tggctgagca ctgtctccca gtatgaccgt
      301 gacaagtact ggaaccgctt ccgagatgaa gttgaggatg actatttcag aaactggaat
      361 cccaacaagc ccttcgacca agccctggac ccatccaagg acccctgcct gaaggtgaaa
      421 tgcagcccgc acaaagtatg tgtgacccag gactaccaga cggctctgtg tgtcagccgc
      481 aagcacctgt tgcccaggca gaagaagggc aatgtggctc acaaacactg gcttggacct
      541 tccaatctgg ttaagtgcaa gccttgcccc gtggcgcagt cagcgatggt ctgcggctct
      601 gacggccaca cgtacacgtc caagtgcaag ttggaattcc acgcttgttc tacaggcaaa
      661 agcctcaact ccctctgtga tgggccctgt ccgtgtctgc ctgagcctga gccactgaag
      721 cccaaagcag agaagagtgc ctgcacggac aaggagctgc ggaacctcgc ctcccggctg
      781 aaggactggt tcggggctct tcatgaggac gccaatagag tcatcaagcc taccagctct
      841 gatccagccc aaggcaggtt tgacaccagc atcttaccca tttgcaagga ctccttgggt
      901 tggatgttca acaagttgga catgaactat gacctgttgc tggaccactc agagatcaat
      961 gccatctacc tagacaaata tgagccctgc atcaagcctc tcttcaactc gtgcgactcc
     1021 ttcaaggacg gcaaactctc caacaatgag tggtgttact gcttccagaa gccagcgggt
     1081 ctcccttgcc agaatgaaat gaacagaatt cagaagctaa gcaaggggaa aagcctactg
     1141 ggggccttca tccctcgatg taacgaggag ggctactaca aagccacaca gtgccacggc
     1201 agcacggggc agtgctggtg tgtggataaa tatgggaacg agctggccgg ctccaggaaa
     1261 cagggcactg taagctgcga agaggagcag gaaacctccg gggacttcgg cagtggaggc
     1321 tccgtggtcc tgctggatga cctagaggat gagcgggagc tgggaccaaa ggacaaagaa
     1381 gggaagctga gggtgcgcac ccgggccgtg cgggaagatg atgaggatga agatgacgac
     1441 aaagaagatg aggtcgggta catatggtag tgcccacgag gaagaggaca cacttttggc
     1501 acagatctgc aagtcgtttc ctttgcctgc atttgtatct aagactccga ggcaccgggg
     1561 tctcttctcc actgttgatc tctgaaccgg ggcctgaggt ttggaagacc ctcttcccag
     1621 agtgactgaa tttgcatacg gttgtgtggg agaaaggatg ttatttttgt tccttgtttt
     1681 cgttttcatt ggatgagagg acaggctggc ttagttaaag ccgcctcctc tccctgtgag
     1741 gttttcgcaa ctagcatgtg atttgtgtgg aatccaacag tgcggggagc cccaccctct
     1801 caagcgtcaa agaccctctc tgattaccca cgtcggtggt tcctacagca tggttcccag
     1861 cgtcttatga tgtctgcgtg cttttctcgt gtcctgtaga tgttgtggaa aacataccag
     1921 gctgtccctt ttccctgtct ctaccacctc tggtgttgat tattaaaaat taattttcat
     1981 gtcactgtat ctgacttcct acaaacgaca gccttaattc agtccaagtc cctttggaga
     2041 attcattttc tttcttaaaa aaaataagat ctcgacaccc cccccccccc cctgcgccca
     2101 aaggtgactg tgcttgcatg acttgcatac acacaccttt cagtctagtg tctggttgta
     2161 taccaaaaac accttagata agcaatggct tttagtcagt tgctccagac cgagaacaca
     2221 aaccggggta tgaaattcct cttagagtcc ggtttgcact agctcttcgt gtgtgtgtgt
     2281 agcctgcatc ccggggaggc tcttctatgc ggtctcagag tgactggaag cctttttgat
     2341 ttggatgagc ctccatttgc ctaaattgtt tttacccaca gaagccatgc aattcccacg
     2401 tgggcagctt cacaagccat ttcttcactt ccgctcacag ctcccctcag gatacagtgt
     2461 acccacctgc ggcagcttcc ttccctatcc acccaagggc tttttcctac cgtgttcccc
     2521 actgagcccc tgctccccaa gaagacagca tcattataaa gcttagaaga aaatttaggg
     2581 ttcttcagtg tctcctttga ccccctttct atccacaaat catgacccag ggtatagaga
     2641 ctttaaaggt ttctcgtgtt cccccattat ttactaggga cccccaatgg aagcccagaa
     2701 agagattcct agtagcacaa gaggctagtc tcctcatcag cagccatctc cccgacacat
     2761 gctgacctta gttgacacac ggctgaggcc attttactgt ttgtcctgag agctcgttat
     2821 tggcatctgg tttcactagt gatatcatct caatactttt cagatttctt tcatacaagt
     2881 accaaagagc aatcttcagt gcgggccccg ttcagctcag ggccaatccc ccagctctgt
     2941 cttcttgctt cctgcctagc tccttgccct aaccagccac agcctgccag acacactctc
     3001 ttcatggtcc ttgccttccc ccatggtgag ttttagccag tccacaagac agatccatca
     3061 actcaggcta caaaggtatt tggttaaacc gagatcaggg aggcagagcc ctaaactggc
     3121 tttctgtatt taggaagcca cctacaattc gaattcccct tcttgaatag gacaggcaaa
     3181 gtgacatcag ccctcttggg aaatgaatat acccggtgcc ctttacccct ggcctgggat
     3241 acacacgcag agccagggtg ctgagattct gtgtgcttcc tccttatgga gatgggaacc
     3301 tgctttggaa tctcagtgcc atcccccccg cccccacccc ctattttgtt tgtttttttc
     3361 ttcccattct caaaatggat cataatttag aaaacaggtg cttaatcaca gctaaattgt
     3421 aatagaatac attaaaaaga catattagaa atctcagttt aggccatcca tataagccat
     3481 tgttgttatt tcctatgagc ttagggggaa aaaaaacatg cacaagagaa caaattaaca
     3541 atcggcaaat tgatttctct ctgagaaact tctaggaacc caatgagcca cttactttgt
     3601 ctaattatct tatgacaagt gtcacattaa gatgacactt agaagtcctc aacattatct
     3661 taatggtgaa gaagagtact caggaggcaa ttcccaggaa ggtaatgaga tgtcatttcc
     3721 agagacattc caagaagata ttttgattca ttaaaatatt aaatcaaaag ccctcctacg
     3781 tgtggagccc acatcaacaa gacaggccag tgccgcttct catggcccca ctttgggttt
     3841 taaagatggc tatttataat ggtactttaa agttagactt ttgttctgtt tcctgagaaa
     3901 gagtgcttcc actggtcttc tggaaggacc ctttcagtct aatcatcagg cgagcatatg
     3961 agacttttta agcatatgcg gctgtaatga taaaccactt gcttaggcag gcactgagct
     4021 gagtccccac ttagctttag aaaaccttca gttgattctg ttactctaat tctacaaact
     4081 gttacttact atcattcctg tgacatccag tttaaaccct agaattctct tccaccaggg
     4141 ttcattgtct gccatatgac tatgtttcta cagatattct cagcatgagg atggcttcac
     4201 atggctttct ctcagtactc ccctttatgc ccacgaccct gtgtatacag gaaatgatga
     4261 tttgtctgtc caggtctacc ctctgcttgt cccagaacgc cattgacatt gactttggtt
     4321 tggtttttat cttgaccaca ctgtacagta acatccaaga gcccttccta cagtgggtgt
     4381 tttttttttt ttttttttgg tctttttaca ccttttctcg gtcagtgatg atgtttgtcc
     4441 ctgttcaaca tgtagccctg taacagaagc ccatcaaatt aattcagagt gtcagtctgt
     4501 tgtccctatt gtaggtgaaa tagtgacgta gaaaacctag tattctgaat gcttttccat
     4561 gtagactttt tctggaatgt gaacacgact cttcagttaa tagcaagcgc ttccctgtag
     4621 ccctgagtag gcgcagttct gtctgtctca ataaatttta ctttgtccgc agca
//
