LOCUS       NP_033288                442 aa            linear   ROD 04-OCT-2024
DEFINITION  testican-1 isoform 1 precursor [Mus musculus].
ACCESSION   NP_033288
VERSION     NP_033288.2
DBSOURCE    REFSEQ: accession NM_009262.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 442)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 442)
  AUTHORS   O'Brown,N.M., Patel,N.B., Hartmann,U., Klein,A.M., Gu,C. and
            Megason,S.G.
  TITLE     The secreted neuronal signal Spock1 promotes blood-brain barrier
            development
  JOURNAL   Dev Cell 58 (17), 1534-1547 (2023)
   PUBMED   37437574
  REMARK    GeneRIF: The secreted neuronal signal Spock1 promotes blood-brain
            barrier development.
REFERENCE   3  (residues 1 to 442)
  AUTHORS   Xie,Z., Ma,X.H., Bai,Q.F., Tang,J., Sun,J.H., Jiang,F., Guo,W.,
            Wang,C.M., Yang,R., Wen,Y.C., Wang,F.Y., Chen,Y.X., Zhang,H.,
            He,D.Z., Kelley,M.W., Yang,S. and Zhang,W.J.
  TITLE     ZBTB20 is essential for cochlear maturation and hearing in mice
  JOURNAL   Proc Natl Acad Sci U S A 120 (24), e2220867120 (2023)
   PUBMED   37279265
REFERENCE   4  (residues 1 to 442)
  AUTHORS   Ebeid,M., Barnas,K., Zhang,H., Yaghmour,A., Noreikaite,G. and
            Bjork,B.C.
  TITLE     PRDM16 expression and function in mammalian cochlear development
  JOURNAL   Dev Dyn 251 (10), 1666-1683 (2022)
   PUBMED   35451126
REFERENCE   5  (residues 1 to 442)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 442)
  AUTHORS   Charbonnier,F., Chanoine,C., Cifuentes-Diaz,C., Gallien,C.L.,
            Rieger,F., Alliel,P.M. and Perin,J.P.
  TITLE     Expression of the proteoglycan SPOCK during mouse embryo
            development
  JOURNAL   Mech Dev 90 (2), 317-321 (2000)
   PUBMED   10640720
REFERENCE   7  (residues 1 to 442)
  AUTHORS   Ringuette,M., Rogers,I., Varmuza,S., Rush,S. and Brown,I.R.
  TITLE     Expression of SC1 is associated with the migration of myotomes
            along the dermomyotome during somitogenesis in early mouse embryos
  JOURNAL   Dev Genes Evol 208 (7), 403-406 (1998)
   PUBMED   9732554
REFERENCE   8  (residues 1 to 442)
  AUTHORS   Sommer,B., Bickel,M., Hofstetter,W. and Wetterwald,A.
  TITLE     Expression of matrix proteins during the development of mineralized
            tissues
  JOURNAL   Bone 19 (4), 371-380 (1996)
   PUBMED   8894143
REFERENCE   9  (residues 1 to 442)
  AUTHORS   Kirschbaum,T., Jaenichen,R. and Zachau,H.G.
  TITLE     The mouse immunoglobulin kappa locus contains about 140 variable
            gene segments
  JOURNAL   Eur J Immunol 26 (7), 1613-1620 (1996)
   PUBMED   8766569
REFERENCE   10 (residues 1 to 442)
  AUTHORS   Bonnet,F., Perin,J.P., Charbonnier,F., Camuzat,A., Roussel,G.,
            Nussbaum,J.L. and Alliel,P.M.
  TITLE     Structure and cellular distribution of mouse brain testican.
            Association with the postsynaptic area of hippocampus pyramidal
            cells
  JOURNAL   J Biol Chem 271 (8), 4373-4380 (1996)
   PUBMED   8626787
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY241184.1, BC075625.1 and AC154748.2.
            
            On Jul 8, 2004 this sequence version replaced NP_033288.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC075625.1, X92864.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 30.5 cM"
     Protein         1..442
                     /product="testican-1 isoform 1 precursor"
                     /note="testican-1"
                     /calculated_mol_wt=47509
     sig_peptide     1..21
                     /note="/evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q62288.2)"
                     /calculated_mol_wt=2120
     mat_peptide     22..442
                     /product="Testican-1. /id=PRO_0000026700"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62288.2)"
                     /calculated_mol_wt=47509
     Region          139..>176
                     /region_name="Kazal_2"
                     /note="Kazal-type serine protease inhibitor domain;
                     pfam07648"
                     /db_xref="CDD:400135"
     Region          201..311
                     /region_name="EFh_SPARC_EC"
                     /note="EF-hand, extracellular calcium-binding (EC) motif,
                     found in secreted protein acidic and rich in cysteine
                     (SPARC)-like proteins; cl25349"
                     /db_xref="CDD:330171"
     Region          248..279
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320009"
     Site            order(260,264,271,292,294,296,303)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320009"
     Region          280..310
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320009"
     Region          315..376
                     /region_name="TY"
                     /note="Thyroglobulin type I repeats.; The N-terminal
                     region of human thyroglobulin contains 11 type-1 repeats
                     TY repeats are proposed to be inhibitors of cysteine
                     proteases; cd00191"
                     /db_xref="CDD:238114"
     Site            order(320,335,353,357)
                     /site_type="active"
                     /note="protease interaction site [active]"
                     /db_xref="CDD:238114"
     Region          375..395
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62288.2)"
     Site            386
                     /site_type="glycosylation"
                     /note="O-linked (Xyl...) (glycosaminoglycan) serine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q62288.2)"
     Site            391
                     /site_type="glycosylation"
                     /note="O-linked (Xyl...) (glycosaminoglycan) serine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q62288.2)"
     Region          420..442
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62288.2)"
     CDS             1..442
                     /gene="Spock1"
                     /gene_synonym="Spock; testican; Ticn1"
                     /coded_by="NM_009262.3:142..1470"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS79195.1"
                     /db_xref="GeneID:20745"
                     /db_xref="MGI:MGI:105371"
ORIGIN      
        1 mpaiavlaaa aaawcflqvd srhldalagg aalnnanfld ndqwlstvsq ydrdkywnrf
       61 rdeveddyfr nwnpnkpfdq aldpskdpcl kvkcsphkvc vtqdyqtalc vsrkhllprq
      121 kkgnvahkhw lgpsnlvkck pcpvaqsamv cgsdghtyts kcklefhacs tgkslnslcd
      181 gpcpclpepe plkpkaeksa ctdkelrnla srlkdwfgal hedanrvikp tssdpaqgrf
      241 dtsilpickd slgwmfnkld mnydllldhs einaiyldky epcikplfns cdsfkdgkls
      301 nnewcycfqk paglpcqnem nriqklskgk sllgafiprc neegyykatq chgstgqcwc
      361 vdkygnelag srkqgtvsce eeqetsgdfg sggsvvlldd lederelgpk dkegklrvrt
      421 ravreddede dddkedevgy iw
//
