LOCUS       NM_012046               1711 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus SPO11 initiator of meiotic double stranded breaks
            (Spo11), transcript variant 1, mRNA.
ACCESSION   NM_012046
VERSION     NM_012046.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Zainu,A., Dupaigne,P., Bouchouika,S., Cau,J., Clement,J.A.J.,
            Auffret,P., Ropars,V., Charbonnier,J.B., de Massy,B., Mercier,R.,
            Kumar,R. and Baudat,F.
  TITLE     FIGNL1-FIRRM is essential for meiotic recombination and prevents
            DNA damage-independent RAD51 and DMC1 loading
  JOURNAL   Nat Commun 15 (1), 7015 (2024)
   PUBMED   39147779
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Kogo,H., Kikuchi-Kokubo,Y., Tajika,Y., Iizuka-Kogo,A., Yamamoto,H.,
            Ikezawa,M., Kurahashi,H. and Matsuzaki,T.
  TITLE     Differential phosphorylation of two serine clusters in mouse
            HORMAD1 during meiotic prophase I progression
  JOURNAL   Exp Cell Res 440 (1), 114133 (2024)
   PUBMED   38897409
  REMARK    GeneRIF: Differential phosphorylation of two serine clusters in
            mouse HORMAD1 during meiotic prophase I progression.
REFERENCE   3  (bases 1 to 1711)
  AUTHORS   Biot,M., Toth,A., Brun,C., Guichard,L., de Massy,B. and Grey,C.
  TITLE     Principles of chromosome organization for meiotic recombination
  JOURNAL   Mol Cell 84 (10), 1826-1841 (2024)
   PUBMED   38657614
REFERENCE   4  (bases 1 to 1711)
  AUTHORS   Bai,L., Li,P., Xiang,Y., Jiao,X., Chen,J., Song,L., Liang,Z.,
            Liu,Y., Zhu,Y. and Lu,L.Y.
  TITLE     BRCA1 safeguards genome integrity by activating chromosome
            asynapsis checkpoint to eliminate recombination-defective oocytes
  JOURNAL   Proc Natl Acad Sci U S A 121 (19), e2401386121 (2024)
   PUBMED   38696471
REFERENCE   5  (bases 1 to 1711)
  AUTHORS   Dereli,I., Telychko,V., Papanikos,F., Raveendran,K., Xu,J.,
            Boekhout,M., Stanzione,M., Neuditschko,B., Imjeti,N.S.,
            Selezneva,E., Tuncay,H., Demir,S., Giannattasio,T., Gentzel,M.,
            Bondarieva,A., Stevense,M., Barchi,M., Schnittger,A., Weir,J.R.,
            Herzog,F., Keeney,S. and Toth,A.
  TITLE     Seeding the meiotic DNA break machinery and initiating
            recombination on chromosome axes
  JOURNAL   Nat Commun 15 (1), 2941 (2024)
   PUBMED   38580643
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1711)
  AUTHORS   Metzler-Guillemain,C. and de Massy,B.
  TITLE     Identification and characterization of an SPO11 homolog in the
            mouse
  JOURNAL   Chromosoma 109 (1-2), 133-138 (2000)
   PUBMED   10855504
REFERENCE   7  (bases 1 to 1711)
  AUTHORS   Shannon,M., Richardson,L., Christian,A., Handel,M.A. and
            Thelen,M.P.
  TITLE     Differential gene expression of mammalian SPO11/TOP6A homologs
            during meiosis
  JOURNAL   FEBS Lett 462 (3), 329-334 (1999)
   PUBMED   10622720
REFERENCE   8  (bases 1 to 1711)
  AUTHORS   Aruga,J., Mizugishi,K., Koseki,H., Imai,K., Balling,R., Noda,T. and
            Mikoshiba,K.
  TITLE     Zic1 regulates the patterning of vertebral arches in cooperation
            with Gli3
  JOURNAL   Mech Dev 89 (1-2), 141-150 (1999)
   PUBMED   10559489
REFERENCE   9  (bases 1 to 1711)
  AUTHORS   Keeney,S., Baudat,F., Angeles,M., Zhou,Z.H., Copeland,N.G.,
            Jenkins,N.A., Manova,K. and Jasin,M.
  TITLE     A mouse homolog of the Saccharomyces cerevisiae meiotic
            recombination DNA transesterase Spo11p
  JOURNAL   Genomics 61 (2), 170-182 (1999)
   PUBMED   10534402
REFERENCE   10 (bases 1 to 1711)
  AUTHORS   Romanienko,P.J. and Camerini-Otero,R.D.
  TITLE     Cloning, characterization, and localization of mouse and human
            SPO11
  JOURNAL   Genomics 61 (2), 156-169 (1999)
   PUBMED   10534401
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL929140.16 and AL928599.5.
            
            On Jan 3, 2023 this sequence version replaced NM_012046.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF169386.1, AF173848.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-189               AL929140.16        117248-117436
            190-303             AL929140.16        119391-119504
            304-392             AL928599.5         2763-2851
            393-459             AL928599.5         3619-3685
            460-568             AL928599.5         4144-4252
            569-655             AL928599.5         4651-4737
            656-692             AL928599.5         5539-5575
            693-802             AL928599.5         5875-5984
            803-902             AL928599.5         7678-7777
            903-940             AL928599.5         8115-8152
            941-1017            AL928599.5         8671-8747
            1018-1129           AL928599.5         10793-10904
            1130-1711           AL928599.5         11731-12312
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 95.64 cM"
     gene            1..1711
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /note="SPO11 initiator of meiotic double stranded breaks"
                     /db_xref="GeneID:26972"
                     /db_xref="MGI:MGI:1349669"
     exon            1..189
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..1249
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /EC_number="5.6.2.2"
                     /note="isoform a is encoded by transcript variant 1;
                     meiotic recombination protein SPO11; SPO11 meiotic protein
                     covalently bound to DSB homolog; sporulation protein,
                     meiosis-specific, SPO11 homolog (S. cervisiae)"
                     /codon_start=1
                     /product="meiotic recombination protein SPO11 isoform a"
                     /protein_id="NP_036176.1"
                     /db_xref="CCDS:CCDS17137.1"
                     /db_xref="GeneID:26972"
                     /db_xref="MGI:MGI:1349669"
                     /translation="MAFAPMGPEASFFDALDRHRASLLAMVKRGAGETPAGATRVASS
                     SEVLTAIENIIQDIIKSLARNEVPAFTIDNRSSWENIMFDDSVGLRMIPQCTTRKIRS
                     DSPKSVKKFALILKVLSMIYKLIQSDTYATKRDIYYTDSQLFGNQAAVDSAIDDISCM
                     LKVPRRSLHVLSTSKGLIAGNLRYMEEDGTRVQCTCSATATAVPTNIQGMQHLITDAK
                     FLLIVEKDATFQRLLDDNFCSRMSPCIMVTGKGVPDLNTRLLVKKLWDTFHIPVFTLV
                     DADPYGIEIMCIYKYGSMSMSFEAHNLTIPTIRWLGLLPSDIQRLNIPKDSLIPLTKH
                     DQMKLDSILKRPYITYQPLWKKELEMMADSKMKAEIQALTLLSSDYLSRVYLPNKLRF
                     GGWI"
     exon            190..303
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     exon            304..392
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     exon            393..459
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     exon            460..568
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     exon            569..655
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     exon            656..692
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     exon            693..802
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     exon            803..902
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     exon            903..940
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     exon            941..1017
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1129
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1711
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1690..1695
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /note="hexamer: AGTAAA"
     polyA_site      1711
                     /gene="Spo11"
                     /gene_synonym="Spo11a; Spo11b"
                     /note="major polyA site"
ORIGIN      
        1 agagcgcgga aaggttcgca ggggcaggag gcctcagagc ggctccgcat ccgccattat
       61 ggcgttcgcg cctatggggc ccgaggcctc gttcttcgac gccctggatc ggcacagggc
      121 ttccctgttg gccatggtga agagaggcgc aggggagacc cctgccgggg ccacccgcgt
      181 ggcctctagt tctgaggttc ttacagctat agaaaatatt atccaagaca taatcaaaag
      241 cttggcaaga aatgaagtgc ctgccttcac aatagacaac agatcgagct gggaaaacat
      301 aatgtttgat gattctgtcg gccttcggat gatacctcag tgtaccacaa gaaaaatcag
      361 aagcgattca ccaaaatcag ttaagaaatt cgctctgatt ctgaaagtat tgtccatgat
      421 ttataaatta atacagagcg acacttatgc aaccaagaga gacatatact acactgacag
      481 ccagctcttt gggaaccagg ctgcggtgga cagcgccatc gatgacattt cctgtatgct
      541 gaaagtgccc aggaggagtc tgcacgtgct atctacttcc aagggattga ttgctggcaa
      601 cttgagatac atggaggaag atggtaccag agtccagtgt acctgtagtg ccacggctac
      661 tgctgtgccg actaacattc aaggaatgca gcatctgatc acagatgcga agtttctgtt
      721 aatagtcgag aaggatgcaa catttcagcg gctcctggac gacaacttct gcagcaggat
      781 gtccccgtgc atcatggtta cgggaaaggg cgttccagat ctgaacacga ggctcttggt
      841 aaagaagctg tgggacacct ttcatattcc tgttttcaca ctggtcgatg cagatcccta
      901 cggcatcgag ataatgtgca tctataagta cggatccatg tccatgtctt ttgaagctca
      961 caatctcact atcccaacaa tcagatggct tggtctcctc ccttctgata tccagaggtt
     1021 aaatatacct aaggatagtt tgatcccact gacaaagcat gaccagatga agctggacag
     1081 catcctgaag aggccttaca ttacctacca gcccctctgg aaaaaagagc tggagatgat
     1141 ggcagactct aagatgaagg cagagatcca agccttgacc ttgctgtcgt cagactacct
     1201 gtccagagtg tacttaccca acaagctgag gtttggagga tggatctaaa atcacagcag
     1261 ggaacgtgga ccaccaggct ttctgttggc ctgcttgaat tacaaacact gggtgaagga
     1321 gtatctagtc ttaggtatgc aatggtaaaa gtaattttac attaacatat ttttgtcaaa
     1381 gcaaacttca tagtgctcta aaaggtttga tcttctgtaa agacagattc ttattctatg
     1441 ttctacagtg attacaaaat tatttaattt aaaattttta ttagaaaaac tgttacatat
     1501 tctaaaagtc tataaaaata tttacttctt gcaacatagc tagtgtagca tgaagtgtct
     1561 cactagcaac aaagaaaaac agcaagcgaa ccttgagagg ttgcgaagga atcacaaggt
     1621 attttcctct agtactttct tttgcctcat ggtataggtg ccttgccctg ttaatgtacc
     1681 tgtgaaacta gtaaagcttg ttataaacaa a
//
