LOCUS       NM_153060               3257 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus SPNS lysolipid transporter 2, sphingosine-1-phosphate
            (Spns2), transcript variant 1, mRNA.
ACCESSION   NM_153060
VERSION     NM_153060.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Martelletti,E., Ingham,N.J. and Steel,K.P.
  TITLE     Reversal of an existing hearing loss by gene activation in Spns2
            mutant mice
  JOURNAL   Proc Natl Acad Sci U S A 120 (34), e2307355120 (2023)
   PUBMED   37552762
REFERENCE   3  (bases 1 to 3257)
  AUTHORS   Hasan,Z., Nguyen,T.Q., Lam,B.W.S., Wong,J.H.X., Wong,C.C.Y.,
            Tan,C.K.H., Yu,J., Thiam,C.H., Zhang,Y., Angeli,V. and Nguyen,L.N.
  TITLE     Postnatal deletion of Spns2 prevents neuroinflammation without
            compromising blood vascular functions
  JOURNAL   Cell Mol Life Sci 79 (11), 541 (2022)
   PUBMED   36198832
  REMARK    GeneRIF: Postnatal deletion of Spns2 prevents neuroinflammation
            without compromising blood vascular functions.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3257)
  AUTHORS   Yasuda,S., Sumioka,T., Miyajima,M., Iwanishi,H., Morii,T.,
            Mochizuki,N., Reinach,P.S., Kao,W.W.Y., Okada,Y., Liu,C.Y. and
            Saika,S.
  TITLE     Anomaly of cornea and ocular adnexa in spinster homolog 2 (Spns2)
            knockout mice
  JOURNAL   Ocul Surf 26, 111-127 (2022)
   PUBMED   35988880
  REMARK    GeneRIF: Anomaly of cornea and ocular adnexa in spinster homolog 2
            (Spns2) knockout mice.
REFERENCE   5  (bases 1 to 3257)
  AUTHORS   Le,T.N.U., Nguyen,T.Q., Kalailingam,P., Nguyen,Y.T.K.,
            Sukumar,V.K., Tan,C.K.H., Tukijan,F., Couty,L., Hasan,Z., Del
            Gaudio,I., Wenk,M.R., Cazenave-Gassiot,A., Camerer,E. and
            Nguyen,L.N.
  TITLE     Mfsd2b and Spns2 are essential for maintenance of blood vessels
            during development and in anaphylactic shock
  JOURNAL   Cell Rep 40 (7), 111208 (2022)
   PUBMED   35977478
  REMARK    GeneRIF: Mfsd2b and Spns2 are essential for maintenance of blood
            vessels during development and in anaphylactic shock.
REFERENCE   6  (bases 1 to 3257)
  AUTHORS   Nijnik,A., Clare,S., Hale,C., Chen,J., Raisen,C., Mottram,L.,
            Lucas,M., Estabel,J., Ryder,E., Adissu,H., Adams,N.C.,
            Ramirez-Solis,R., White,J.K., Steel,K.P., Dougan,G. and
            Hancock,R.E.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     The role of sphingosine-1-phosphate transporter Spns2 in immune
            system function
  JOURNAL   J Immunol 189 (1), 102-111 (2012)
   PUBMED   22664872
  REMARK    GeneRIF: The changes in lymphocyte subpopulations in mutant Spns2
            mice closely mimick the phenotypes of partial
            sphingosine-1-phosphate (S1P) deficiency, impaired S1P-dependent
            lymphocyte trafficking, and depletion of circulating lymphocytes.
REFERENCE   7  (bases 1 to 3257)
  AUTHORS   Fukuhara,S., Simmons,S., Kawamura,S., Inoue,A., Orba,Y.,
            Tokudome,T., Sunden,Y., Arai,Y., Moriwaki,K., Ishida,J., Uemura,A.,
            Kiyonari,H., Abe,T., Fukamizu,A., Hirashima,M., Sawa,H., Aoki,J.,
            Ishii,M. and Mochizuki,N.
  TITLE     The sphingosine-1-phosphate transporter Spns2 expressed on
            endothelial cells regulates lymphocyte trafficking in mice
  JOURNAL   J Clin Invest 122 (4), 1416-1426 (2012)
   PUBMED   22406534
  REMARK    GeneRIF: Spns2 functions in endothelial cells to establish the S1P
            gradient required for T and B cells to egress from their respective
            primary lymphoid organs.
REFERENCE   8  (bases 1 to 3257)
  AUTHORS   Hisano,Y., Kobayashi,N., Yamaguchi,A. and Nishi,T.
  TITLE     Mouse SPNS2 functions as a sphingosine-1-phosphate transporter in
            vascular endothelial cells
  JOURNAL   PLoS One 7 (6), e38941 (2012)
   PUBMED   22723910
  REMARK    GeneRIF: SPNS2 is the first physiological S1P transporter in
            mammals and is a key determinant of lymphocyte egress from the
            thymus.
REFERENCE   9  (bases 1 to 3257)
  AUTHORS   Hisano,Y., Kobayashi,N., Kawahara,A., Yamaguchi,A. and Nishi,T.
  TITLE     The sphingosine 1-phosphate transporter, SPNS2, functions as a
            transporter of the phosphorylated form of the immunomodulating
            agent FTY720
  JOURNAL   J Biol Chem 286 (3), 1758-1766 (2011)
   PUBMED   21084291
  REMARK    GeneRIF: The sphingosine 1-phosphate transporter, SPNS2, functions
            as a transporter of the phosphorylated form of the immunomodulating
            agent FTY720.
REFERENCE   10 (bases 1 to 3257)
  AUTHORS   Kawahara,A., Nishi,T., Hisano,Y., Fukui,H., Yamaguchi,A. and
            Mochizuki,N.
  TITLE     The sphingolipid transporter spns2 functions in migration of
            zebrafish myocardial precursors
  JOURNAL   Science 323 (5913), 524-527 (2009)
   PUBMED   19074308
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL662812.12.
            
            On Feb 5, 2013 this sequence version replaced NM_153060.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC011467.1, SRR7345562.3797823.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-495               AL662812.12        107117-107611       c
            496-561             AL662812.12        92396-92461         c
            562-698             AL662812.12        81334-81470         c
            699-850             AL662812.12        76593-76744         c
            851-917             AL662812.12        76324-76390         c
            918-1060            AL662812.12        74896-75038         c
            1061-1213           AL662812.12        74466-74618         c
            1214-1403           AL662812.12        74191-74380         c
            1404-1469           AL662812.12        73994-74059         c
            1470-1568           AL662812.12        71991-72089         c
            1569-1732           AL662812.12        71745-71908         c
            1733-1779           AL662812.12        71245-71291         c
            1780-3257           AL662812.12        69329-70806         c
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 44.31 cM"
     gene            1..3257
                     /gene="Spns2"
                     /note="SPNS lysolipid transporter 2,
                     sphingosine-1-phosphate"
                     /db_xref="GeneID:216892"
                     /db_xref="MGI:MGI:2384936"
     exon            1..495
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..1775
                     /gene="Spns2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein spinster homolog 2; spinster2; spinster-like
                     protein; sphingosine-1-phosphate transporter SPNS2"
                     /codon_start=1
                     /product="sphingosine-1-phosphate transporter SPNS2
                     isoform 1"
                     /protein_id="NP_694700.2"
                     /db_xref="CCDS:CCDS48841.1"
                     /db_xref="GeneID:216892"
                     /db_xref="MGI:MGI:2384936"
                     /translation="MMCLECASAAAGGAEEEEADAERRRRRRGAQPGAGGSACCGARG
                     VGGAGVVSADEEVQTLSGSVRRVPSGLPSIPSTPGCAAAAKGPSAPQPKPASLGRGRG
                     AAAAILSLGNVLNYLDRYTVAGVLLDIQQHFGVKDRGAGLLQSVFICSFMVAAPIFGY
                     LGDRFNRKVILSCGIFFWSAVTFSSSFIPQQYFWLLVLSRGLVGIGEASYSTIAPTII
                     GDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQAAGDWHWALRVSPVLGMITGTL
                     ILILVPATKRGHADQLGGQLKARTSWLRDMKALIRNRSYVFSSLATSAVSFATGALGM
                     WIPLYLHRAQVVQKTAETCNSPPCGAKDSLIFGAITCFTGFLGVVTGAGATRWCRLRT
                     QRADPLVCAVGMLGSAIFICLIFVAAKTSIVGAYICIFVGETLLFSNWAITADILMYV
                     VIPTRRATAVALQSFTSHLLGDAGSPYLIGFISDLIRQSTKDSPLWEFLSLGYALMLC
                     PFVVVLGGMFFLATALFFLSDRAKAEQQVNQLVMPPASVKV"
     exon            496..561
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     exon            562..698
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     exon            699..850
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     exon            851..917
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1060
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1213
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1403
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1469
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1568
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1732
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1779
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..3257
                     /gene="Spns2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3238..3243
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spns2"
                     /note="hexamer: AATAAA"
     polyA_site      3257
                     /gene="Spns2"
                     /note="major polyA site"
ORIGIN      
        1 cggacgggcc gggccgggat ggtgcagcgg cggctccgcg gcgcgcgcac gcactgagcg
       61 ggcccgggct gggcctcgcg tcccccgagc cccccgcgcc ccccgccgcc ccggcccggg
      121 ccggcatgat gtgcctggaa tgcgcctcgg cggcggcggg cggcgcggag gaggaggagg
      181 cggacgccga gcggcggcgc cggcgccggg gggcgcagcc aggggctgga ggtagcgctt
      241 gctgcggggc gcggggtgtt gggggcgctg gagtcgtgtc cgccgacgaa gaggtgcaga
      301 cgttgtccgg cagcgtgagg cgggtcccgt ccggactccc cagcatcccc agcacccctg
      361 gctgcgcagc agctgccaag ggccccagcg cgccgcagcc caaaccggcc agcttaggcc
      421 gagggcgggg ggcagctgct gccatcctga gtttaggcaa cgtgctcaac tacctggaca
      481 ggtacactgt ggcaggtgtt cttctggaca tccagcagca ctttggggtc aaggaccggg
      541 gcgccggctt gctgcagtca gtgttcatct gcagcttcat ggtggctgcc cccatctttg
      601 gctacctggg cgaccgcttc aatagaaagg tgatcctcag ctgtggcatc ttcttctggt
      661 ctgctgtcac cttctcgagt tctttcatcc ctcagcagta cttctggcta ctagtcctgt
      721 cccgagggct agtaggtatt ggtgaagcca gctactccac catagcaccc accatcattg
      781 gcgacttgtt taccaagaac acacgcacat tgatgctatc tgtcttctat tttgccatcc
      841 ccctgggcag tggcctgggc tatatcacag gttccagcgt gaagcaggca gctggagact
      901 ggcattgggc cctgcgggtt tcccccgtcc tgggcatgat cacaggaaca ctcatcctca
      961 tcttggtccc agccactaag agaggccatg ctgatcaact tggggggcag ctcaaagcac
     1021 ggacctcctg gcttcgagac atgaaggccc tgatccgaaa ccgcagttac gtcttctcct
     1081 ccctggccac atccgctgtg tccttcgcca caggggccct gggcatgtgg attcccctct
     1141 atcttcaccg tgctcaagtt gtacaaaaga cagcagagac ttgcaacagc ccgccctgtg
     1201 gagccaaaga cagcctcatc tttggagcca ttacctgctt tactggcttc ctgggcgtag
     1261 tcacgggcgc gggagccact cgttggtgcc gcctgcggac tcaacgtgct gacccattgg
     1321 tgtgtgctgt gggcatgctg ggatccgcca tcttcatctg cctcatcttt gtggctgcca
     1381 agaccagcat tgtaggcgct tatatctgca tttttgttgg agagacactg ctgttttcta
     1441 actgggccat cactgcagac atcctcatgt atgtggtcat ccccactcgg cgagccactg
     1501 ctgtggccct gcagagcttc acctcccatc tgctggggga cgctggaagc ccctacctca
     1561 ttggctttat ctcggaccta atccgccaga gcaccaagga ctccccgctc tgggagttcc
     1621 tgagcctggg ctatgccctc atgctgtgcc cttttgtcgt ggtcctgggt ggcatgttct
     1681 tccttgccac tgctctcttc ttcctcagcg accgtgccaa ggctgagcag caggtgaacc
     1741 agctggtgat gcctcctgca tccgtgaaag tctgaggtgg tgccgctggg acagcaatga
     1801 actgcagttt aggttgggag aggttctcca gcatcctgga ccggcaggac ggtcccaaag
     1861 cttcctgtgt aactcacagt tgggtaccca ccctctttgg cctgggaccg ccaggactga
     1921 gtggccctat cttcaagaag aagccgcatc ctcagttagc ctggaaggct gtgtgtgctg
     1981 gaaccactca gttggacaca actccagccc tggctgggga ctggagggcc aagatagaag
     2041 ccaccgtgca gcagggattt ggggagattc tggcctgccc ttagctcata tgatcttgag
     2101 ggagccactg ccgtcctcct tcgacagtag aaacaaggag ctggagcttc ctccattgtg
     2161 tgccatgcaa ggcacactcc acagctttga agaaacaggt cctgggctat acacagaaga
     2221 catggctcag gtctcgggac ctcagttcca gttctgaggt ctgccaaagg ctctggtgta
     2281 tggggaccac ttccccactc ttaggctcta accttgggaa cagcaaattc tgcctggcat
     2341 atgtagctgg gtatccactt ttgggtgttt ctggctggga cagtctccca ggataccatc
     2401 tctgctgtct tcagctagtt ctttgcctgg aggttttctc aggtgggagc acccccttaa
     2461 agaaagctgc ggcactaggg ggtgaaggcc ctggtggtag gctgagaact tgaccttttc
     2521 ttaccccagc cccccaactc ctacagtacc ccagaagcca cctcatccta caggatgcag
     2581 gatgacccaa gagtcctttc caatgaggag tcagataaga tgctaaagga cttacccagc
     2641 tgtccacact acttggccta gggtataaaa ctgctggaga ttgcccacca gtgcctatag
     2701 gtatcatacc ccaactgact cttggggccg ggccttcacc ccagcctttg atcacacttc
     2761 ccccacacac acaacccctc actcccctgc agccacctta gtcacctttg ggcacttatc
     2821 cccagcttta cactccttgc acgctgtaac caaggtggct ctggtcctgg ggtaccatta
     2881 caacttgcag gaaacttcca gaaccagctt gggtaacaac agtccctgtg gctgtatccc
     2941 acttgctgtg gcttcagtgg tgttagttag tggtacccac cccaccaaac tctggggcac
     3001 cctgggggtt tgggcgtggg tttgcagagg gtgctatccc taaagaccag cctggctccc
     3061 accctaaccc cagcaggggc cctagcaatg ttttcttgtt gtacaagaac caggtccaag
     3121 tgttgcctcc tcttccttcc ggaagctgaa ctgctccttt attttttaga gctgctgatt
     3181 gtgaatctca aagtcttaag agagaagcca aatatatatt cctcttgtaa atgaataaat
     3241 aaacctattt aaatgtt
//
