LOCUS       NM_023712               2706 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus SPNS lysolipid transporter 1, lysophospholipid
            (Spns1), transcript variant 1, mRNA.
ACCESSION   NM_023712
VERSION     NM_023712.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Beenken,A., Shen,T., Jin,G., Ghotra,A., Xu,K., Nesanir,K.,
            Sturley,R.E., Vijayakumar,S., Khan,A., Levitman,A., Stauber,J.,
            Chavez,E.Y., Robbins-Juarez,S.Y., Hao,L., Field,T.B.,
            Erdjument-Bromage,H., Neubert,T.A., Shapiro,L., Qiu,A. and
            Barasch,J.
  TITLE     Spns1 is an iron transporter essential for megalin-dependent
            endocytosis
  JOURNAL   Am J Physiol Renal Physiol 327 (5), F775-F787 (2024)
   PUBMED   39265081
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Ha,H.T., Liu,S., Nguyen,X.T., Vo,L.K., Leong,N.C., Nguyen,D.T.,
            Balamurugan,S., Lim,P.Y., Wu,Y., Seong,E., Nguyen,T.Q., Oh,J.,
            Wenk,M.R., Cazenave-Gassiot,A., Yapici,Z., Ong,W.Y., Burmeister,M.
            and Nguyen,L.N.
  TITLE     Lack of SPNS1 results in accumulation of lysolipids and lysosomal
            storage disease in mouse models
  JOURNAL   JCI Insight 9 (8), e175462 (2024)
   PUBMED   38451736
  REMARK    GeneRIF: Lack of SPNS1 results in accumulation of lysolipids and
            lysosomal storage disease in mouse models.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2706)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2706)
  AUTHORS   He,M., Kuk,A.C.Y., Ding,M., Chin,C.F., Galam,D.L.A., Nah,J.M.,
            Tan,B.C., Yeo,H.L., Chua,G.L., Benke,P.I., Wenk,M.R., Ho,L.,
            Torta,F. and Silver,D.L.
  TITLE     Spns1 is a lysophospholipid transporter mediating lysosomal
            phospholipid salvage
  JOURNAL   Proc Natl Acad Sci U S A 119 (40), e2210353119 (2022)
   PUBMED   36161949
  REMARK    GeneRIF: Spns1 is a lysophospholipid transporter mediating
            lysosomal phospholipid salvage.
REFERENCE   5  (bases 1 to 2706)
  AUTHORS   Yanagisawa,H., Ishii,T., Endo,K., Kawakami,E., Nagao,K.,
            Miyashita,T., Akiyama,K., Watabe,K., Komatsu,M., Yamamoto,D. and
            Eto,Y.
  TITLE     L-leucine and SPNS1 coordinately ameliorate dysfunction of
            autophagy in mouse and human Niemann-Pick type C disease
  JOURNAL   Sci Rep 7 (1), 15944 (2017)
   PUBMED   29162837
  REMARK    GeneRIF: SPNS1-dependent L-leucine export across the lysosomal
            membrane is a key step for triggering autophagy, and that this
            mechanism is impaired in Niemann-Pick type C disease-1.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2706)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   7  (bases 1 to 2706)
  AUTHORS   Nakano,Y., Fujitani,K., Kurihara,J., Ragan,J., Usui-Aoki,K.,
            Shimoda,L., Lukacsovich,T., Suzuki,K., Sezaki,M., Sano,Y., Ueda,R.,
            Awano,W., Kaneda,M., Umeda,M. and Yamamoto,D.
  TITLE     Mutations in the novel membrane protein spinster interfere with
            programmed cell death and cause neural degeneration in Drosophila
            melanogaster
  JOURNAL   Mol Cell Biol 21 (11), 3775-3788 (2001)
   PUBMED   11340170
  REMARK    GeneRIF: Spinster protein interferes with programmed cell death in
            Drosophila melanogaster and has orthologs in nematode, mouse, and
            human.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY731880.1, AF212372.1, AK166931.1 and CR519967.1.
            
            On Nov 18, 2011 this sequence version replaced NM_023712.2.
            
            Transcript Variant: This variant (1) represents a protein-coding
            transcript for this gene.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026854.1, AK166931.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-631               BY731880.1         2-632
            632-1235            AF212372.1         123-726
            1236-2591           AK166931.1         728-2083
            2592-2706           CR519967.1         1-115               c
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 69.01 cM"
     gene            1..2706
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /note="SPNS lysolipid transporter 1, lysophospholipid"
                     /db_xref="GeneID:73658"
                     /db_xref="MGI:MGI:1920908"
     exon            1..1098
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     CDS             858..2444
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /note="spinster-like protein; spinster homolog 1; MSpin1"
                     /codon_start=1
                     /product="protein spinster homolog 1"
                     /protein_id="NP_076201.2"
                     /db_xref="CCDS:CCDS21826.1"
                     /db_xref="GeneID:73658"
                     /db_xref="MGI:MGI:1920908"
                     /translation="MAGSDTAPFLSQADDPDDGPAPGHPGLPGPMGNPKSGELEVPDC
                     EGLQRITGLSRGHSTLIVVVLCYINLLNYMDRFTVAGVLTDIEQFFNIGDGSTGLIQT
                     VFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPREHFWLLLLTRGLV
                     GVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDVAGDWHW
                     ALRVTPGLGVLAVLLLFLVVQEPPRGAVERHSGSPPLSPTSWWADLKALARNPSFVLS
                     SLGFTSVAFVTGSLALWAPAFLLRSRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGV
                     LGVGLGVEISRRLRRFNPRADPLVCAAGLLGSAPFLFLALACARGSIVATYIFIFIGE
                     TLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLISDRLRRSWP
                     PSFLSEFRALQFSLMLCAFVGALGGAAFLGTAMFIEDDRRRAQLHVQGLLHESGPSDD
                     RIVVPQRGRSTRVPVSSVLI"
     exon            1099..1164
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     exon            1165..1301
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1453
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1520
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1666
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1822
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..2012
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     exon            2013..2078
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     exon            2079..2177
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     exon            2178..2349
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     exon            2350..2703
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2678..2683
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /note="hexamer: AATAAA"
     polyA_site      2696
                     /gene="Spns1"
                     /gene_synonym="2210013K02Rik; Spin1; Spinl"
                     /note="major polyA site"
ORIGIN      
        1 agtatgtagc cctggctgac ctggaactcg ctatgtaaac caggctgtcc ttgaaattgc
       61 aaagatctcc gtccttctgc cttctcctgg gactaaaggt atgcgctact acgcccgact
      121 ctataggaat ctaaatgtcc acagtgaaag ctcatgattg gtaaacaaaa ggaggcagct
      181 gctcgccgct attcatccaa caattactga gcaatgaagc catcagccag tcactgaaga
      241 agaaaagagg aaacaactca ctagcatctg tagagatgta atatttccaa ataatccact
      301 atctcgtgaa atcggataaa tgggtattcc atctaggcga ttgattacac cagcttgcga
      361 ggccccctgg aggctactgg tggaagtagg aacgagcgtc gggttaccat agcggaggag
      421 ccctagaaac cctggaggct tccgtaggcg gtggcaggtc acgtgttcaa tccttgcacc
      481 gggtgacttt ctccggtagg gctccggtgt cacatgacct gctcttgggc aacatggcgg
      541 cagttgtgtt gctgagcttg gactgagcaa cagcgagtgt ggcgcgctcc ttcccgggct
      601 ttggagtgcg tcagcgtgag aagagggact gggtcctggt cttcctgctc ctgtcccagc
      661 gtcacctgca cctcctgtgt gctctcctct gtcggaacca gagggaatga cgaagcccag
      721 ggcttttcgc agtggtgcac tgtgcatcgg accacgctcc taggcgatag tgggcaagtc
      781 ttctcgcagt cctctctcca acctccttcc gtcggaggta ggaccgaagc gtgggcggtt
      841 gcgattcccc agggaccatg gccgggtccg acacggcgcc cttcctcagc caagcagatg
      901 atcctgatga cgggccagcg cccggccatc cggggttgcc aggacccatg gggaatccaa
      961 agtccgggga actcgaggtc ccagactgtg aggggctaca gcgcatcact ggcttatctc
     1021 ggggccattc gaccctcata gtggtggttc tgtgctacat taacctcctg aactacatgg
     1081 accgcttcac cgtggcaggg gttcttacag acatcgagca gttctttaac atcggagatg
     1141 gtagtactgg cctcatccag actgtgttca tctccagtta catggtgttg gcaccagtgt
     1201 ttggctacct gggtgacagg tacaatcgaa agtacctcat gtgcgggggc attgccttct
     1261 ggtccctggt gacactggga tcatccttca tccccagaga gcatttctgg ctgcttctcc
     1321 tgacccgggg cctggtgggg gtcggggagg ccagttactc caccattgcg cccaccctga
     1381 tcgccgacct cttcgtggca gaccagcgga gtcggatgct cagtatcttc tactttgcca
     1441 tccctgtggg cagtggtcta ggttacattg ctggctccaa agtgaaagac gtggctggag
     1501 actggcactg ggctctacgg gtgacaccag gtctaggagt gctggctgtc ctgctgctgt
     1561 tcctggtggt ccaggagccc ccaagaggag ccgtggagcg ccactcaggt tcaccacccc
     1621 tgagccccac ctcttggtgg gcagatctga aggcactggc acgaaatcct agtttcgtcc
     1681 tgtcttccct tggcttcacc tctgtggcct ttgtcacggg ctccctggct ctctgggccc
     1741 cagcgttcct gctgcgctcc cgggttgttc tgggagagac tccgccctgt ctccctggag
     1801 attcatgctc ttcctctgac agtctcatct ttggactcat cacttgcctg actggagtcc
     1861 tgggtgtggg cctgggagtg gagatcagcc gccgccttcg ccgcttcaac cctcgggctg
     1921 acccactcgt ctgtgcagct ggcctcctgg gttcggcgcc tttcctcttc ctggccctgg
     1981 cctgtgcccg aggtagcatc gtggccacct atatttttat ctttattggg gagaccctgt
     2041 tgtccatgaa ctgggccatt gtggctgaca tcctgttgta cgtggtgatc ccaactcgac
     2101 ggtccacggc tgaggccttc cagatagtgc tgtcccactt gctaggagat gcagggagcc
     2161 cttacctcat tggtctaatc tctgaccgcc tccgacggag ctggccccct tccttcctgt
     2221 ccgagttccg ggctctgcag ttctcgctca tgctctgtgc tttcgttggg gcactgggtg
     2281 gtgcggcctt cctgggcacc gccatgttca ttgaagatga ccgccggcgg gctcaactcc
     2341 acgtgcaggg tctgttgcat gagtctgggc cctcagatga ccggattgta gtacctcagc
     2401 gaggccgttc tacccgagtc cccgtgtcca gcgtgctcat ctgaggagcc ggtgcttacc
     2461 cggccactga tgcatcgcag ctgggccttg ggcccaccca agacggttcc caggcagaag
     2521 ccctcaccag gcccaggtcc aagaaggaag ccctgggata tctcccagct cccagacact
     2581 acatgggcag cccagggaag agatgggagt ccagaaacgg ggaaggggtg tcctctctac
     2641 taggacagcc caaggggttt ggtgctattt gtaatggaat aaaatttgta atcagactcc
     2701 tgaaaa
//
