LOCUS       NM_001253777            1119 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus sperm microtubule inner protein 6 (Spmip6), transcript
            variant 3, mRNA.
ACCESSION   NM_001253777 NR_045548
VERSION     NM_001253777.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Zhou,L., Liu,H., Liu,S., Yang,X., Dong,Y., Pan,Y., Xiao,Z.,
            Zheng,B., Sun,Y., Huang,P., Zhang,X., Hu,J., Sun,R., Feng,S.,
            Zhu,Y., Liu,M., Gui,M. and Wu,J.
  TITLE     Structures of sperm flagellar doublet microtubules expand the
            genetic spectrum of male infertility
  JOURNAL   Cell 186 (13), 2897-2910 (2023)
   PUBMED   37295417
REFERENCE   3  (bases 1 to 1119)
  AUTHORS   Pleuger,C., Lehti,M.S., Cooper,M., O'Connor,A.E., Merriner,D.J.,
            Smyth,I.M., Cottle,D.L., Fietz,D., Bergmann,M. and O'Bryan,M.K.
  TITLE     CBE1 Is a Manchette- and Mitochondria-Associated Protein With a
            Potential Role in Somatic Cell Proliferation
  JOURNAL   Endocrinology 160 (11), 2573-2586 (2019)
   PUBMED   31504408
  REMARK    GeneRIF: localized to the mitochondria and the contractile ring of
            dividing inner medullary collecting duct cells; associated with
            intramanchette transport and midpiece formation of the sperm tail;
            long isoforms not essential for male fertility
REFERENCE   4  (bases 1 to 1119)
  AUTHORS   Stauber,M., Weidemann,M., Dittrich-Breiholz,O., Lobschat,K.,
            Alten,L., Mai,M., Beckers,A., Kracht,M. and Gossler,A.
  TITLE     Identification of FOXJ1 effectors during ciliogenesis in the foetal
            respiratory epithelium and embryonic left-right organiser of the
            mouse
  JOURNAL   Dev Biol 423 (2), 170-188 (2017)
   PUBMED   27914912
REFERENCE   5  (bases 1 to 1119)
  AUTHORS   Miyata,H., Castaneda,J.M., Fujihara,Y., Yu,Z., Archambeault,D.R.,
            Isotani,A., Kiyozumi,D., Kriseman,M.L., Mashiko,D., Matsumura,T.,
            Matzuk,R.M., Mori,M., Noda,T., Oji,A., Okabe,M.,
            Prunskaite-Hyyrylainen,R., Ramirez-Solis,R., Satouh,Y., Zhang,Q.,
            Ikawa,M. and Matzuk,M.M.
  TITLE     Genome engineering uncovers 54 evolutionarily conserved and
            testis-enriched genes that are not required for male fertility in
            mice
  JOURNAL   Proc Natl Acad Sci U S A 113 (28), 7704-7710 (2016)
   PUBMED   27357688
REFERENCE   6  (bases 1 to 1119)
  AUTHORS   Haitchi,H.M., Yoshisue,H., Ribbene,A., Wilson,S.J., Holloway,J.W.,
            Bucchieri,F., Hanley,N.A., Wilson,D.I., Zummo,G., Holgate,S.T. and
            Davies,D.E.
  TITLE     Chronological expression of Ciliated Bronchial Epithelium 1 during
            pulmonary development
  JOURNAL   Eur Respir J 33 (5), 1095-1104 (2009)
   PUBMED   19213785
  REMARK    GeneRIF: Chronological regulation of CBE1/Cbe1 expression during
            pulmonary differentiation suggests involvement in ciliogenesis,
            with an additional role during early lung development.
REFERENCE   7  (bases 1 to 1119)
  AUTHORS   Matsuoka,Y., Miyagawa,Y., Tokuhiro,K., Kitamura,K., Iguchi,N.,
            Maekawa,M., Takahashi,T., Tsujimura,A., Matsumiya,K., Okuyama,A.,
            Nishimune,Y. and Tanaka,H.
  TITLE     Isolation and characterization of the spermatid-specific Smrp1 gene
            encoding a novel manchette protein
  JOURNAL   Mol Reprod Dev 75 (6), 967-975 (2008)
   PUBMED   18163442
  REMARK    GeneRIF: SMRP1 was localized to the cytoplasm of step 9-12
            elongated spermatids. The protein appeared in a cap formation that
            covered the caudal sides of the elongated nuclei.
REFERENCE   8  (bases 1 to 1119)
  AUTHORS   McClintock,T.S., Glasser,C.E., Bose,S.C. and Bergman,D.A.
  TITLE     Tissue expression patterns identify mouse cilia genes
  JOURNAL   Physiol Genomics 32 (2), 198-206 (2008)
   PUBMED   17971504
  REMARK    GeneRIF: 1110017D15Rik is most abundantly expressed in tissues rich
            in highly ciliated cells, such as olfactory sensory neurons, and is
            predicted to be important to cilia.
REFERENCE   9  (bases 1 to 1119)
  AUTHORS   Yoshisue,H., Puddicombe,S.M., Wilson,S.J., Haitchi,H.M.,
            Powell,R.M., Wilson,D.I., Pandit,A., Berger,A.E., Davies,D.E.,
            Holgate,S.T. and Holloway,J.W.
  TITLE     Characterization of ciliated bronchial epithelium 1, a ciliated
            cell-associated gene induced during mucociliary differentiation
  JOURNAL   Am J Respir Cell Mol Biol 31 (5), 491-500 (2004)
   PUBMED   15242845
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL831723.9.
            
            On Dec 3, 2011 this sequence version replaced NR_045548.1.
            
            Transcript Variant: This variant (3) uses an alternate splice site
            that results in a frameshift in the 3' coding region, compared to
            variant 1. The encoded isoform (3) has a distinct and longer
            C-terminus, compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB231294.1, SRR5189685.49714.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-306               AL831723.9         43037-43342         c
            307-451             AL831723.9         37441-37585         c
            452-564             AL831723.9         34526-34638         c
            565-637             AL831723.9         33530-33602         c
            638-872             AL831723.9         33103-33337         c
            873-959             AL831723.9         31577-31663         c
            960-1119            AL831723.9         31018-31177         c
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 21.7 cM"
     gene            1..1119
                     /gene="Spmip6"
                     /gene_synonym="1110017D15Rik; Cbe1; Smrp1"
                     /note="sperm microtubule inner protein 6"
                     /db_xref="GeneID:73721"
                     /db_xref="MGI:MGI:1920971"
     exon            1..306
                     /gene="Spmip6"
                     /gene_synonym="1110017D15Rik; Cbe1; Smrp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             172..1062
                     /gene="Spmip6"
                     /gene_synonym="1110017D15Rik; Cbe1; Smrp1"
                     /note="isoform 3 is encoded by transcript variant 3;
                     ciliated bronchial epithelium 1; testes
                     development-related NYD-SP22; likely ortholog of H.
                     sapiens chromosome 9 open reading frame 24 (C9orf24);
                     spermatid-specific manchette-related protein 1; ciliated
                     bronchial epithelial protein 1; testis development protein
                     NYD-SP22"
                     /codon_start=1
                     /product="sperm microtubule inner protein 6 isoform 3"
                     /protein_id="NP_001240706.1"
                     /db_xref="GeneID:73721"
                     /db_xref="MGI:MGI:1920971"
                     /translation="MFLFSRKTKTPISTYSDSYRAPTSIKEVYKDPPLWAWEANKFVT
                     PGLTQTMHRHVDPEALQKMTKCAAQDYTYKSSISGHPYLPEKYWLSPDEEDKCCPSYL
                     DNDRYNTWKTSPCSNYWNKYTGCLPRLSKDTGMESVRGMPLEYPPKQERLNAYEREVV
                     VNMLNSLSRNRTLPQIVPRCGCVDPLPGRLPYQGYESPCSGRHYCLRGMDYCTTREPS
                     TERRLRPLCSQQPTVRSVSPFGHRPGMQCAVTTPPPSFYPYPNLRWDTSHFKKTGGSQ
                     RNNYVVHPEFVSETCPVYPS"
     exon            307..451
                     /gene="Spmip6"
                     /gene_synonym="1110017D15Rik; Cbe1; Smrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            452..564
                     /gene="Spmip6"
                     /gene_synonym="1110017D15Rik; Cbe1; Smrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            565..637
                     /gene="Spmip6"
                     /gene_synonym="1110017D15Rik; Cbe1; Smrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            638..872
                     /gene="Spmip6"
                     /gene_synonym="1110017D15Rik; Cbe1; Smrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            873..959
                     /gene="Spmip6"
                     /gene_synonym="1110017D15Rik; Cbe1; Smrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1119
                     /gene="Spmip6"
                     /gene_synonym="1110017D15Rik; Cbe1; Smrp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1098..1103
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spmip6"
                     /gene_synonym="1110017D15Rik; Cbe1; Smrp1"
                     /note="hexamer: AATAAA"
     polyA_site      1117
                     /gene="Spmip6"
                     /gene_synonym="1110017D15Rik; Cbe1; Smrp1"
                     /note="major polyA site"
ORIGIN      
        1 aagaaagctg agggggaaga tactgagagg tcccccaaag gaaggggtca cagcatctag
       61 gggcaccttc caggccggag gtgtgggcta tttaggggaa ggggtagtca cagatgtcca
      121 gcaggagtcc ttgagaggca aagcccacta cagcatccct gagaccctac catgttcctc
      181 ttctcccgga agaccaagac ccccatcagc acctacagtg actcctacag ggctcctacc
      241 tccatcaagg aggtctataa ggacccacca ctatgggcct gggaagccaa caagtttgtg
      301 accccaggtc tgactcagac tatgcaccgc cacgtggacc ctgaggccct gcagaagatg
      361 accaaatgtg ccgcacagga ctacacctat aagagttcca tatcaggtca cccttatttg
      421 cctgagaaat actggctctc tccagatgaa gaggacaaat gctgtccgag ctacctggac
      481 aatgaccggt acaacacgtg gaagacgagt ccttgcagca actactggaa caagtatacc
      541 ggctgtcttc ctcgactgtc caaggacacc gggatggagt cagttcgagg aatgccgttg
      601 gaatatcctc ctaagcagga gcgcctcaac gcctacgagc gcgaggtagt ggtgaacatg
      661 ctgaactcgc tgtctcggaa ccggactctg ccgcagattg taccccgctg tgggtgcgtg
      721 gaccctctcc cgggccgatt gccataccaa ggatatgaaa gcccttgctc cggccgccac
      781 tactgtctgc gcgggatgga ctactgcacc acccgggagc ctagcacaga acgccgcctg
      841 cggcctttgt gctcgcagca gccgactgta aggagtgtgt cgcccttcgg tcaccggcca
      901 ggaatgcaat gtgctgttac aactcccccg ccatcatttt acccgtatcc caaccttaga
      961 tgggacacaa gtcacttcaa gaagactggt ggttcccaga gaaacaacta tgtagtccat
     1021 cctgagtttg tgtctgagac ctgtcctgtc tacccttcct agtaaagctt tgaccaggcc
     1081 aagggaggaa gtggctcaat aaactcttca ccacaaaga
//
