LOCUS       NM_001417718            2530 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus sperm microtubule inner protein 4 (Spmip4), transcript
            variant 2, mRNA.
ACCESSION   NM_001417718
VERSION     NM_001417718.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Sun,J., Lu,Y., Nozawa,K., Xu,Z., Morohoshi,A., Castaneda,J.M.,
            Noda,T., Miyata,H., Abbasi,F., Shawki,H.H., Takahashi,S.,
            Devlin,D.J., Yu,Z., Matzuk,R.M., Garcia,T.X., Matzuk,M.M. and
            Ikawa,M.
  TITLE     CRISPR/Cas9-based genome editing in mice uncovers 13 testis- or
            epididymis-enriched genes individually dispensable for male
            reproductiondagger
  JOURNAL   Biol Reprod 103 (2), 183-194 (2020)
   PUBMED   32588039
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153386.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14777530.595444.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-155               AC153386.3         19905-20059
            156-498             AC153386.3         20362-20704
            499-675             AC153386.3         24689-24865
            676-747             AC153386.3         27320-27391
            748-777             AC153386.3         29295-29324
            778-959             AC153386.3         31978-32159
            960-1048            AC153386.3         32484-32572
            1049-1223           AC153386.3         38537-38711
            1224-1315           AC153386.3         38990-39081
            1316-2530           AC153386.3         41986-43200
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 24.32 cM"
     gene            1..2530
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /note="sperm microtubule inner protein 4"
                     /db_xref="GeneID:70821"
                     /db_xref="MGI:MGI:1918071"
     exon            1..155
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /inference="alignment:Splign:2.1.0"
     exon            156..498
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /inference="alignment:Splign:2.1.0"
     CDS             365..2131
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /note="uncharacterized protein C7orf31 homolog; sperm
                     associated microtubule inner protein 4"
                     /codon_start=1
                     /product="sperm-associated microtubule inner protein 4"
                     /protein_id="NP_001404647.1"
                     /db_xref="GeneID:70821"
                     /db_xref="MGI:MGI:1918071"
                     /translation="MEVIHGRPHCCRNLEGADILSNTFYSNGLHTPYETTIRPTASQA
                     RYQELREALPQCRLRWGADREYGGVLPVSLPEEHRPKCEPPRLMSKGHQHYGFGGEIW
                     PKKLPIEQYYYMTQNKKSDVYGNDSLLPKPPNSAVKEICSPYPIEHPYHTHISRGSVF
                     PTFTSPKDLYTGIKARTQQPFPPTVPTKACDTTILKTRGNPYRYELLDFPMDSKKKAL
                     TWPGQGVYYDFPKFVEKNKPVFYPKPPKTFAPNSSVNPWDTMSSAKDANIQRNLERSH
                     WLTSYAHDFTGLGPMSPLELDDYHEKELAELTGQIGFDPQPQEKFHPALKTPRPLDGR
                     IARLTQNQRPLEATVQIPPPCPDCTPRVLCAFHTFIPTSAEIMAMNNNLLSGITHKNQ
                     DVEEKIKEEQGLMSTCPLPTCYESKDLTSLYDVQSFPKITDTKKTDDLYWRQLEMKPL
                     PISCSKSNHYIDYEPLKSAYRDPYAMCPNPVRLSKSNILQNKTDTADFTFDNFLSKPE
                     FLGMNMESNEETRPLLDWIPRAGVPKHHSNLRNLRNTFSKSMAQKRLHNSIQEEQKDL
                     RDKLQCGMRHQFFGYNGHHFYN"
     exon            499..675
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /inference="alignment:Splign:2.1.0"
     exon            676..747
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /inference="alignment:Splign:2.1.0"
     exon            748..777
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /inference="alignment:Splign:2.1.0"
     exon            778..959
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1048
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1223
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1315
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..2530
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2162..2167
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /note="hexamer: AATAAA"
     polyA_site      2183
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /note="major polyA site"
     regulatory      2509..2514
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
                     /note="hexamer: AATAAA"
     polyA_site      2530
                     /gene="Spmip4"
                     /gene_synonym="4921507P07Rik; TISP74"
ORIGIN      
        1 ctctggcctt cttcccccgc acctgcccgc acgctcccgg ggcccgcagg tgcggaagac
       61 cacgaaattc cacgccccgc ggagaggaga cccaccagat cccgagacaa gaaagggaga
      121 cgcggtcggg cagcgctccg gggcagatgt gtcaggtttg cttcagttta tctccacagg
      181 tttgacagca tccacagacc ctcacagcca gtaagaaaat aagcctctgg tttttgtgca
      241 tcgatgagtt gaattttgcc agcagaaact gtttcctgtc cggtctaacc aaggcagttt
      301 acaagtccaa attttcattt ttatctattt acttatctcc agactaacat tttaggcaag
      361 aagcatggag gtaattcacg gcaggcccca ctgctgcagg aaccttgaag gagctgacat
      421 tctgtcaaac accttttact ctaacggact gcacaccccg tacgaaacaa ctattcggcc
      481 aactgcttca caagccaggt atcaggagct gagggaggct ctgccacaat gcagacttcg
      541 gtggggtgcc gatagagaat atggtggtgt gcttccagtt tcccttcccg aagaacacag
      601 gccgaaatgt gagcctcccc gtctcatgag caaaggacac cagcattacg gatttggcgg
      661 ggagatctgg ccaaaaaagc tccccattga gcagtattac tatatgacac agaacaagaa
      721 gagcgacgtg tatggaaacg actctttgct acccaaacca cctaattcag cagtaaaaga
      781 aatctgctca ccgtatccaa ttgaacatcc ctaccataca cacataagcc gtggctccgt
      841 gtttccgacc tttacatcgc ctaaggacct ttacaccggc attaaagctc gtacccagca
      901 gccttttcct cctacggtgc caaccaaggc ctgtgacaca accattctga agacaagagg
      961 taacccttac agatatgaac tgctcgattt tcccatggat tcaaagaaaa aagcattgac
     1021 ttggccaggt cagggtgtat attatgattt tcctaaattt gttgagaaaa acaagccagt
     1081 attctacccc aaacctccta aaacctttgc tcctaactcc tctgtgaatc catgggatac
     1141 aatgagctct gcaaaggacg ctaatataca gagaaatctt gagaggtctc actggctcac
     1201 ttcatatgct cacgatttta caggtctagg tcctatgagc ccccttgaac tggatgatta
     1261 ccatgaaaag gagctagcag agctaactgg acagatagga tttgacccac agcctcaaga
     1321 aaaattccat cctgccttaa aaactcctag acctttggat ggtcgaattg cccgacttac
     1381 tcagaaccaa cgacctctgg aggctacagt gcaaatacca cctccctgtc cagactgtac
     1441 ccctagagtt ttgtgtgctt ttcatacatt catacccact tctgcagaaa ttatggcaat
     1501 gaataataac ctcctgtctg gaataaccca taaaaaccag gatgttgaag agaagattaa
     1561 ggaagaacaa ggcttgatgt ctacctgtcc actcccaact tgttatgaat caaaagactt
     1621 gactagtctc tatgacgtac aatcatttcc aaagatcaca gacactaaaa agacagatga
     1681 tttgtactgg agacagctgg agatgaagcc cctccccata tcctgctcta agtcaaacca
     1741 ttacattgac tatgaaccac ttaagtctgc ctaccgtgac ccatatgcta tgtgtccaaa
     1801 ccctgttcgc ctcagtaagt ctaatattct tcaaaacaaa acagatacgg ctgatttcac
     1861 ttttgacaac tttttaagta aaccagaatt tttgggcatg aacatggaaa gcaatgaaga
     1921 aacaagacct cttttagatt ggatccctag agctggagtg cccaagcatc attccaacct
     1981 gcgaaatctt aggaacacat tctcaaagtc catggcacag aagcgtctgc ataactccat
     2041 tcaagaagaa caaaaagacc tccgagataa actgcagtgt gggatgagac atcaattttt
     2101 tggctacaat ggccatcatt tctataattg agattccctt gagacccagt ctcttgcaag
     2161 aaataaaata tgtcacagag tgacctgcat ttggctggat tctgtttctg tattaagcca
     2221 taatgacatt aatgaggttg tgttttcaag aatttaagtt aggatctggt caaagggacc
     2281 aaaataatgt cctgacttct caaaacattt aagtttgtca acaaaatatc agagccagaa
     2341 aaaaaatagt gtttaatctc atcttcaaat tatactctga agttttccaa aatcatgaaa
     2401 atcaatgtgt agacaaaatt cacatcatca ttttgtgaga aatgaaaaaa ttgaaaaaaa
     2461 atcaaatatt gaagatttga cagacagcta tagaatgtat catgttttaa taaaattagt
     2521 atatcaacaa
//
