LOCUS       NM_001370773            1889 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus sperm microtubule inner protein 2 (Spmip2), transcript
            variant 1, mRNA.
ACCESSION   NM_001370773 XM_011239978
VERSION     NM_001370773.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. PMID: 29144450]
REFERENCE   3  (bases 1 to 1889)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC142502.3.
            
            On May 31, 2019 this sequence version replaced XM_011239978.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.153463.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-170               AC142502.3         141533-141702       c
            171-403             AC142502.3         81159-81391         c
            404-462             AC142502.3         67654-67712         c
            463-1889            AC142502.3         55725-57151         c
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 34.91 cM"
     gene            1..1889
                     /gene="Spmip2"
                     /gene_synonym="Gm17359"
                     /note="sperm microtubule inner protein 2"
                     /db_xref="GeneID:100233207"
                     /db_xref="MGI:MGI:4936993"
     exon            1..170
                     /gene="Spmip2"
                     /gene_synonym="Gm17359"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..1573
                     /gene="Spmip2"
                     /gene_synonym="Gm17359"
                     /note="isoform 1 is encoded by transcript variant 1; sperm
                     associated microtubule inner protein 2"
                     /codon_start=1
                     /product="protein SPMIP2 isoform 1"
                     /protein_id="NP_001357702.1"
                     /db_xref="CCDS:CCDS89638.1"
                     /db_xref="GeneID:100233207"
                     /db_xref="MGI:MGI:4936993"
                     /translation="MALSHREPHCETVGKRMIVTGPDYVKDHLPKVHQHTAYIGEKRP
                     ALEKTGDLRYLWRPASNRSLPAKYKHEYTCGIGWGIPQYSFFNRSRVESGFHIQHGEL
                     SLRAMDKITHRYQNPCKQEHGPWGSSWMLVTNSAGRKISDPDKDLGGSPDLGHQQVLR
                     GPIRPLTSVLPQAAAPPEDINKASGGSADHGPPPGLQLLTSPPDINTDSGRTTDPDRA
                     PSSSPGVDYVSSGDSTGMSSDWYGPQRLCRPLTSIWPPPAARPRDICMISGHPHGLPL
                     FTCSTDSDYSRTTDPDLVLSSCTVPNINMVSRGHRGYSDRYGPQRLRRPLTSIWPPAV
                     ARPKNINMDSSSSLEHGHPHGLQLLTSPKDINTDSGCSRTTDPDMALSSSMSPDTNMI
                     SGGGRGYTEWHGHLKLRSPQTPMWPQVAPQPTDVCMTSGGNTHLTVDINKDPRFSTMQ
                     CRADGAMDNTVASDGSPAQGYQPGFRLKYRLRRSAWS"
     exon            171..403
                     /gene="Spmip2"
                     /gene_synonym="Gm17359"
                     /inference="alignment:Splign:2.1.0"
     exon            404..462
                     /gene="Spmip2"
                     /gene_synonym="Gm17359"
                     /inference="alignment:Splign:2.1.0"
     exon            463..1889
                     /gene="Spmip2"
                     /gene_synonym="Gm17359"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1806..1811
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spmip2"
                     /gene_synonym="Gm17359"
                     /note="hexamer: AATACA"
     polyA_site      1824
                     /gene="Spmip2"
                     /gene_synonym="Gm17359"
     regulatory      1865..1870
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spmip2"
                     /gene_synonym="Gm17359"
                     /note="hexamer: AATAAA"
     polyA_site      1889
                     /gene="Spmip2"
                     /gene_synonym="Gm17359"
                     /note="major polyA site"
ORIGIN      
        1 agttcacaga caaggagagc agagacacgg tctttgccgt ttactggtgc actcactgtt
       61 ctgaagacgt tcaggcagta attgcagcaa agaaatttaa acttcagcca tggctttgtc
      121 acaccgggag ccacactgtg agacggtggg aaagcgcatg atcgtcacag gtccagacta
      181 cgtaaaagac cacttgccta aagttcacca gcataccgcc tatataggag aaaagcgtcc
      241 agcattagag aaaactggag atctcagata tttatggagg cctgcttcaa acagaagcct
      301 gccggcaaaa tataaacacg agtacacctg tggaattggt tgggggatac cacagtacag
      361 tttcttcaac agatcaagag tggaaagtgg ctttcatatc cagcatggcg aactaagtct
      421 ccgcgcgatg gacaaaataa cccaccgcta tcaaaaccca tgtaagcagg agcatggacc
      481 atggggttcc tcatggatgc tggtgacgaa ctcagctggc aggaagatct ccgacccaga
      541 caaggacctc gggggcagcc cagacctcgg acaccaacaa gttctcaggg gtccgatcag
      601 accactcaca tcagtattgc cccaagctgc agcaccaccc gaggacatca acaaggcttc
      661 aggtggcagc gcagaccacg gacctccccc cggccttcag ttgttaacat cccccccaga
      721 catcaacaca gactcgggga ggaccacaga cccagacagg gcccccagta gcagcccagg
      781 tgtagattat gtgtcttcag gtgacagtac aggcatgtcc tcagattggt atggtcccca
      841 gaggctgtgc aggccactca catcaatatg gcccccacct gcggcacgac ccagggacat
      901 ctgcatgatt tcaggacatc cacatggcct cccgttgttc acatgctcca cagactccga
      961 ctacagtcgg accacagacc cagatttggt cctcagtagc tgcacagtcc caaatataaa
     1021 catggtttca cgtggccaca gagggtactc agataggtat ggcccccaga ggcttcgcag
     1081 gccactcaca tcaatatggc ccccagctgt agcacgaccc aagaacatca acatggattc
     1141 aagttccagc ttagaacacg gacatccaca tggccttcag ttgttaacat cccccaagga
     1201 catcaacaca gactctggct gtagtagaac cacagaccca gacatggccc tcagtagcag
     1261 catgagtcca gataccaaca tgatttcagg tggtggcaga ggctacacag agtggcatgg
     1321 ccacctgaag cttcgcagcc cacagacacc aatgtggcct caggtggcac cacagcccac
     1381 agatgtctgc atgacatcag gaggtaacac acacctaacc gtagacatca acaaagaccc
     1441 cagattcagt accatgcagt gccgtgcaga tggagccatg gataacaccg tggcctcaga
     1501 tggcagccca gctcagggat atcaacccgg cttcaggctg aagtatagac taaggagatc
     1561 tgcatggagt taggtaggat aaaggccaaa gacatcaacc cggccccctg gccacatcag
     1621 gaccactgac ccacgcatgg ccctcagtga catcaaggac catttctgcc tcttcccaca
     1681 gcatatgttc tgctgcaccc ttctgccctt cccccatccc cactgctcat ttattcggtc
     1741 atcgtggtgc ctgccagcct ccaacctgtc cagcttccac cagcttgtgc cccatttttt
     1801 tccctaatac aatgcttttt aacattttgt tctttatttt tgagaatttc atatatatgt
     1861 acacaataaa atatgatcat atcaactga
//
