LOCUS       NM_172287               2399 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus spire type actin nucleation factor 2 (Spire2), mRNA.
ACCESSION   NM_172287
VERSION     NM_172287.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Poncet,N., Halley,P.A., Lipina,C., Gierlinski,M., Dady,A.,
            Singer,G.A., Febrer,M., Shi,Y.B., Yamaguchi,T.P., Taylor,P.M. and
            Storey,K.G.
  TITLE     Wnt regulates amino acid transporter Slc7a5 and so constrains the
            integrated stress response in mouse embryos
  JOURNAL   EMBO Rep 21 (1), e48469 (2020)
   PUBMED   31789450
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Jo,Y.J., Lee,I.W., Jung,S.M., Kwon,J., Kim,N.H. and Namgoong,S.
  TITLE     Spire localization via zinc finger-containing domain is crucial for
            the asymmetric division of mouse oocyte
  JOURNAL   FASEB J 33 (3), 4432-4447 (2019)
   PUBMED   30557038
  REMARK    GeneRIF: After injection of double-strand RNA against Spire 1 and 2
            (Spire1+2 double-strand RNA), actin mesh levels were significantly
            decreased compared with that of control (negative).  Conserved
            zinc-binding cysteines in the FYVE domain of Spire2 are crucial for
            proper localization in the oocyte cortex and cytoplasmic vesicles.
            The interaction between FMN2 and Spire2 proteins does not affect
            localization of Spire2.
REFERENCE   3  (bases 1 to 2399)
  AUTHORS   Pylypenko,O., Welz,T., Tittel,J., Kollmar,M., Chardon,F.,
            Malherbe,G., Weiss,S., Michel,C.I., Samol-Wolf,A., Grasskamp,A.T.,
            Hume,A., Goud,B., Baron,B., England,P., Titus,M.A., Schwille,P.,
            Weidemann,T., Houdusse,A. and Kerkhoff,E.
  TITLE     Coordinated recruitment of Spir actin nucleators and myosin V
            motors to Rab11 vesicle membranes
  JOURNAL   Elife 5, e17523 (2016)
   PUBMED   27623148
  REMARK    GeneRIF: The authors determined crystal structures of
            MyoVa-globular tail domain bound either to the Spir-2 motif or to
            Rab11 and show that a Spir-2:MyoVa:Rab11 complex can form.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2399)
  AUTHORS   Pleiser,S., Banchaabouchi,M.A., Samol-Wolf,A., Farley,D., Welz,T.,
            Wellbourne-Wood,J., Gehring,I., Linkner,J., Faix,J.,
            Riemenschneider,M.J., Dietrich,S. and Kerkhoff,E.
  TITLE     Enhanced fear expression in Spir-1 actin organizer mutant mice
  JOURNAL   Eur J Cell Biol 93 (5-6), 225-237 (2014)
   PUBMED   24345451
REFERENCE   5  (bases 1 to 2399)
  AUTHORS   Wisby,L., Fernandes,V.E., Neill,D.R., Kadioglu,A., Andrew,P.W. and
            Denny,P.
  TITLE     Spir2; a novel QTL on chromosome 4 contributes to susceptibility to
            pneumococcal infection in mice
  JOURNAL   BMC Genomics 14, 242 (2013)
   PUBMED   23577770
  REMARK    GeneRIF: We describe a novel locus, called Streptococcus pneumoniae
            infection resistance 2 (Spir2) on Chr4, which influences time to
            morbidity and the development of bacteraemia post-infection.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2399)
  AUTHORS   Schuh,M.
  TITLE     An actin-dependent mechanism for long-range vesicle transport
  JOURNAL   Nat Cell Biol 13 (12), 1431-1436 (2011)
   PUBMED   21983562
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2399)
  AUTHORS   Pfender,S., Kuznetsov,V., Pleiser,S., Kerkhoff,E. and Schuh,M.
  TITLE     Spire-type actin nucleators cooperate with Formin-2 to drive
            asymmetric oocyte division
  JOURNAL   Curr Biol 21 (11), 955-960 (2011)
   PUBMED   21620703
  REMARK    GeneRIF: Spire1 and Spire2 drive two critical steps of asymmetric
            oocyte division
REFERENCE   8  (bases 1 to 2399)
  AUTHORS   Pleiser,S., Rock,R., Wellmann,J., Gessler,M. and Kerkhoff,E.
  TITLE     Expression patterns of the mouse Spir-2 actin nucleator
  JOURNAL   Gene Expr Patterns 10 (7-8), 345-350 (2010)
   PUBMED   20723621
  REMARK    GeneRIF: Spir-2 is found to be expressed as a single mRNA in the
            developing nervous system and intestine. In adult mouse it is
            expressed in epithelial cells of the digestive tract, in neuronal
            cells of the nervous system, and in testical spermatocytes.
REFERENCE   9  (bases 1 to 2399)
  AUTHORS   Schumacher,N., Borawski,J.M., Leberfinger,C.B., Gessler,M. and
            Kerkhoff,E.
  TITLE     Overlapping expression pattern of the actin organizers Spir-1 and
            formin-2 in the developing mouse nervous system and the adult brain
  JOURNAL   Gene Expr Patterns 4 (3), 249-255 (2004)
   PUBMED   15053972
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC049152.1.
            
            On Jun 19, 2003 this sequence version replaced NM_172287.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC054072.1, SRR7652917.305941.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 72.1 cM"
     gene            1..2399
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /note="spire type actin nucleation factor 2"
                     /db_xref="GeneID:234857"
                     /db_xref="MGI:MGI:2446256"
     exon            1..308
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..2209
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /note="spire homolog 2"
                     /codon_start=1
                     /product="protein spire homolog 2"
                     /protein_id="NP_758491.1"
                     /db_xref="CCDS:CCDS22754.1"
                     /db_xref="GeneID:234857"
                     /db_xref="MGI:MGI:2446256"
                     /translation="MARAGGGGAAAPERAGGAARPEPWELSLEEVLKVYEQPINEEQA
                     WAVCFQGCRGLRGEPGGVRRIRDTADILLRRDGSVGARLEPEPTTMVVPPASSEAQMV
                     QSLGFAIYRALDWGLDENEERELSPQLERLIDLMANSDCEDSSCGAADEGYVGPEEEE
                     EAEGGPRAVRTFAQAMRLCALRLTDPHGAQAHYQAVCRALFVETLELRAFLARVREAK
                     EMLKKLGEEEPREKPLAELDHLGHTDWARLWVQLMRELRHGVKLKKVQEKEFNPLPTE
                     FQLTPFEMLMQDIRARNYKLRKVMVDGDIPPRVKKDAHELILDFIRSRPPLKQVSERQ
                     LRPVPQKQRTLHEKILEEIKQERRLRPVGAQHLGGRGFGSLPCILNACSGDIKSTSCI
                     NLSVTDTGSGSQRPRPRVLLKAPTLAEMEEMNTSEEEESPCGEVALKRDRSFSEHDLA
                     QLRSEMASGLQSAAQPPGGTEPPRARAGSMHSWRPSSRDQGFCPVSGQSQPLPSSALP
                     SSLSSVDGPEAASPDTRHLWLEFSHPVESLALTVEEVVDVRRVLVKAEMERFLQDKEL
                     FSSLKRGKICCCCRAKFPLFSWPPTCLFCKRAVCTSCSVKMKMPSKKYGHIPVYTLGF
                     ESLQRVPTTKATPTLRRDAFQSLQGPKWRSVEEEFPHIYAHGCVLKDVCSDCTSFVAD
                     VVCSSRKSVDVLNATPRRSRQTQSLYIPNTRTLNFQ"
     exon            309..352
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            353..709
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            710..787
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            788..952
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1039
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1163
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1333
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1523
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1639
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            1640..1774
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1842
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..1870
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            1871..1986
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     exon            1987..2333
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2309..2314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /note="hexamer: AATATA"
     polyA_site      2333
                     /gene="Spire2"
                     /gene_synonym="Spir-2; Spir2"
                     /note="major polyA site"
ORIGIN      
        1 ccccgctgtt atggagaccc gccgcgcgcg cgggcggcga tgatgggcgg ccatggcccg
       61 ggcgggcggc ggcggcgcag cagctcccga acgcgcgggg ggcgcggcgc ggcccgagcc
      121 gtgggagctg tccctggagg aagtgctgaa ggtgtacgag cagcccatca acgaggagca
      181 ggcctgggcg gtgtgcttcc agggctgccg cgggctgcgg ggcgagccgg gcggcgtgcg
      241 gcgcatccgg gacacggcag acatcctcct gcgtagggac ggttcggttg gcgcgcggct
      301 ggagcccgag cctacaacca tggtggtacc accagccagc tcagaagccc agatggtgca
      361 gtcactgggc tttgccattt accgtgcgct ggactggggg ttagatgaga acgaggagag
      421 agagctcagc ccgcagctgg aaaggctcat cgacctcatg gccaacagcg actgcgaaga
      481 cagcagctgc ggggcagcgg atgagggcta cgtgggtcca gaagaggagg aggaggctga
      541 gggcggcccc cgagctgtgc gcacctttgc ccaggctatg cggctgtgtg ctttgcgcct
      601 gaccgacccc cacggcgcgc aggcccacta ccaggcagtg tgccgagctc tctttgtgga
      661 gacactggag cttcgtgcct tcctggcccg ggtgagggag gccaaagaga tgctgaagaa
      721 actgggggag gaggagccac gggagaagcc actggccgag ctcgaccacc tgggacacac
      781 agactgggcc cggctgtggg tccagctcat gcgggagctc cgtcacgggg taaagctgaa
      841 gaaggtgcag gagaaggagt tcaacccact gcccaccgag ttccagctca cccccttcga
      901 gatgctgatg caggatatcc gtgccaggaa ctataagctg cgcaaggtca tggtcgatgg
      961 ggacatccct cccagggtga agaaggacgc acatgaactt atcctggact tcattcgttc
     1021 tcggcctcca ctgaagcagg tctcagagag acaacttcgc cctgtaccac agaagcaaag
     1081 aacactgcac gagaagatcc tggaggaaat caagcaggag cggaggcttc gtccagtcgg
     1141 ggcccagcac ttgggaggcc gtgggtttgg ttccctgccc tgcatcctca atgcctgctc
     1201 tggggacatc aagtccactt cctgcatcaa cctgtctgtc accgatactg ggagcgggag
     1261 tcagcgccca cggccccggg tcctgctcaa agcccccacc cttgcagaga tggaagaaat
     1321 gaacacgtct gaggaagaag agtccccatg tggggaggtg gcactgaagc gggaccgctc
     1381 tttttcggaa cacgacctgg cccagcttcg gagcgagatg gcatctggcc ttcagtcagc
     1441 cgctcagccc ccaggaggga cggagccacc tcgggccaga gcaggtagca tgcactcctg
     1501 gaggcccagc agccgagacc agggtttctg tcctgtgagc ggccagtccc agcctctccc
     1561 cagctctgcc ttgcccagta gcttgagctc agtggatgga cctgaggcgg cttcaccaga
     1621 taccagacac ctgtggctgg agttcagcca ccctgtggag agcctggctc tgactgtgga
     1681 agaggtggtg gacgtgcgca gggtgctggt gaaggcggag atggagaggt ttctgcagga
     1741 caaggagctc ttcagcagcc tgaagagggg gaagatttgc tgttgctgcc gagccaagtt
     1801 tcccctgttt tcctggccac ccacctgtct cttctgcaag agagctgtgt gcacttcctg
     1861 tagcgtaaag atgaagatgc cttccaagaa gtatggacac atccctgtct acacacttgg
     1921 ctttgagagt cttcagaggg tgccaactac caaagccacg ccaacactga ggagagatgc
     1981 cttccaatcc ctgcagggac caaagtggcg gagcgtggag gaggagttcc cccacatcta
     2041 tgcccacggc tgtgtcctaa aggatgtctg cagtgactgc accagctttg tggctgatgt
     2101 cgtgtgctcc agccgcaaga gcgtggatgt cctcaatgcc actccacgac gaagccgtca
     2161 gacccagtcc ctctatatcc ccaacaccag gactctcaac ttccaatgac tgttgtgatg
     2221 gcctccccac accccatcct ggaacacagc tgccatcagc ccccccaccc ttgcgcatgt
     2281 acatatatac atatatagat acatttataa tatatatatg gcctatattt ataaaaaaaa
     2341 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
//
