LOCUS       NM_030058                746 bp    mRNA    linear   ROD 17-FEB-2024
DEFINITION  Mus musculus serine protease inhibitor, Kunitz type 4 (Spint4),
            mRNA.
ACCESSION   NM_030058
VERSION     NM_030058.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Penttinen,J., Pujianto,D.A., Sipila,P., Huhtaniemi,I. and
            Poutanen,M.
  TITLE     Discovery in silico and characterization in vitro of novel genes
            exclusively expressed in the mouse epididymis
  JOURNAL   Mol Endocrinol 17 (11), 2138-2151 (2003)
   PUBMED   12920233
  REMARK    GeneRIF: Spint4 (serine protease inhibitor, Kunitz type 4), and
            Rnase9 and Rnase10 (ribonuclease, Rnase A family 9 and 10), while
            for the ESTs AV381130 and AV381126 no putative functions could be
            predicted
REFERENCE   2  (bases 1 to 746)
  AUTHORS   Sivashanmugam,P., Hall,S.H., Hamil,K.G., French,F.S., O'Rand,M.G.
            and Richardson,R.T.
  TITLE     Characterization of mouse Eppin and a gene cluster of similar
            protease inhibitors on mouse chromosome 2
  JOURNAL   Gene 312, 125-134 (2003)
   PUBMED   12909348
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AL591127.12.
            
            On Nov 17, 2006 this sequence version replaced NM_030058.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY226988.1, BB073296.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               AL591127.12        16593-16757
            166-355             AL591127.12        18238-18427
            356-542             AL591127.12        18886-19072
            543-746             AL591127.12        20337-20540
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 85.26 cM"
     gene            1..746
                     /gene="Spint4"
                     /gene_synonym="9230105I15Rik"
                     /note="serine protease inhibitor, Kunitz type 4"
                     /db_xref="GeneID:78239"
                     /db_xref="MGI:MGI:1925489"
     exon            1..165
                     /gene="Spint4"
                     /gene_synonym="9230105I15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..530
                     /gene="Spint4"
                     /gene_synonym="9230105I15Rik"
                     /note="kunitz-type protease inhibitor 4; epididymal
                     trypsin inhibitor Spint4"
                     /codon_start=1
                     /product="kunitz-type protease inhibitor 4 precursor"
                     /protein_id="NP_084334.1"
                     /db_xref="CCDS:CCDS17051.1"
                     /db_xref="GeneID:78239"
                     /db_xref="MGI:MGI:1925489"
                     /translation="MKPTKLGFLLGLSLLCSLSPPVLSGVERLANYLCKDYNDPCLLD
                     VEPGSCYEVHFRFFYNQTAKQCQIFLFTGCNGNLNNFKLKIDCDVTCHETYKNPPLPS
                     GDKRKRSLRALATKNLATDWRLTTLQPGRLRQTERKETLLQRARRESRRQPLARHHT"
     exon            166..355
                     /gene="Spint4"
                     /gene_synonym="9230105I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            356..542
                     /gene="Spint4"
                     /gene_synonym="9230105I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            543..746
                     /gene="Spint4"
                     /gene_synonym="9230105I15Rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtataagag cagttggcaa cagactctcc tctctgctgc ttgctccacc atgaagccca
       61 ctaagctggg atttctcctg ggactctccc tcctctgctc actcagtcct ccagtactga
      121 gcggtgttga gaggctagct aactatctct gcaaagacta caacgatccc tgtctgttgg
      181 atgtggagcc tggcagctgc tatgaagtac attttagatt tttctacaac caaactgcca
      241 aacagtgtca gatttttctg ttcactggct gcaatggcaa ccttaataac ttcaagctga
      301 aaatagactg tgatgtaact tgtcacgaaa catacaaaaa ccctccgctg ccgagcggcg
      361 ataagaggaa gcgttctcta agagcattgg caaccaagaa ccttgctacg gattggagat
      421 tgaccacttt acagcctggt agactgaggc agacggaaag aaaagagacc ttgctacaga
      481 gagcacggag agagtccaga aggcaacccc tggccaggca tcacacctag gcagctgcaa
      541 agaaaatccg aacctgaatc tgaacctgaa ctgaaaagca cggtgtgttc gtggaggttc
      601 tcggatgggc catctgaaat aaagattcaa gaagattcag accaatgttt aggtttttgg
      661 atcgtgtcca taatctttga cacttctatt cactattttc ctgttcctaa ttttgtcttt
      721 cctgaaataa aaattactac catcac
//
