LOCUS       NM_011464               1477 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus serine protease inhibitor, Kunitz type 2 (Spint2),
            transcript variant 1, mRNA.
ACCESSION   NM_011464
VERSION     NM_011464.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Szabo,R., Kawaguchi,M., Kataoka,H. and Bugge,T.H.
  TITLE     Early-onset tufting enteropathy in HAI-2-deficient mice is
            independent of matriptase-mediated cleavage of EpCAM
  JOURNAL   Development 150 (17) (2023)
   PUBMED   37539662
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1477)
  AUTHORS   Wilkerson,B.A., Zebroski,H.L., Finkbeiner,C.R., Chitsazan,A.D.,
            Beach,K.E., Sen,N., Zhang,R.C. and Bermingham-McDonogh,O.
  TITLE     Novel cell types and developmental lineages revealed by single-cell
            RNA-seq analysis of the mouse crista ampullaris
  JOURNAL   Elife 10, e60108 (2021)
   PUBMED   34003106
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1477)
  AUTHORS   Szabo,R., Callies,L.K. and Bugge,T.H.
  TITLE     Matriptase drives early-onset intestinal failure in a mouse model
            of congenital tufting enteropathy
  JOURNAL   Development 146 (22) (2019)
   PUBMED   31628112
  REMARK    GeneRIF: matriptase is a causative agent in the development of
            congenital tufting enteropathy (CTE) and may provide a new target
            for the treatment of CTE in individuals carrying SPINT2 mutation
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1477)
  AUTHORS   Kawaguchi,M., Yamamoto,K., Takeda,N., Fukushima,T., Yamashita,F.,
            Sato,K., Kitamura,K., Hippo,Y., Janetka,J.W. and Kataoka,H.
  TITLE     Hepatocyte growth factor activator inhibitor-2 stabilizes Epcam and
            maintains epithelial organization in the mouse intestine
  JOURNAL   Commun Biol 2, 11 (2019)
   PUBMED   30623107
  REMARK    GeneRIF: Hai-2 is an essential protease inhibitor for epithelial
            integrity, Epcam homeostasis and mucosal organization of the
            intestinal tract.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1477)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 1477)
  AUTHORS   Kataoka,H., Itoh,H., Nuki,Y., Hamasuna,R., Naganuma,S., Kitamura,N.
            and Shimomura,T.
  TITLE     Mouse hepatocyte growth factor (HGF) activator inhibitor type 2
            lacking the first Kunitz domain potently inhibits the HGF activator
  JOURNAL   Biochem Biophys Res Commun 290 (3), 1096-1100 (2002)
   PUBMED   11798188
REFERENCE   8  (bases 1 to 1477)
  AUTHORS   Mitchell,K.J., Pinson,K.I., Kelly,O.G., Brennan,J., Zupicich,J.,
            Scherz,P., Leighton,P.A., Goodrich,L.V., Lu,X., Avery,B.J.,
            Tate,P., Dill,K., Pangilinan,E., Wakenight,P., Tessier-Lavigne,M.
            and Skarnes,W.C.
  TITLE     Functional analysis of secreted and transmembrane proteins critical
            to mouse development
  JOURNAL   Nat Genet 28 (3), 241-249 (2001)
   PUBMED   11431694
REFERENCE   9  (bases 1 to 1477)
  AUTHORS   Itoh,H., Kataoka,H., Meng,J.Y., Hamasuna,R., Kitamura,N. and
            Koono,M.
  TITLE     Mouse hepatocyte growth factor activator inhibitor type 1 (HAI-1)
            and type 2 (HAI-2)/placental bikunin genes and their promoters
  JOURNAL   Biochim Biophys Acta 1519 (1-2), 92-95 (2001)
   PUBMED   11406276
REFERENCE   10 (bases 1 to 1477)
  AUTHORS   Itoh,H., Kataoka,H., Hamasuna,R., Kitamura,N. and Koono,M.
  TITLE     Hepatocyte growth factor activator inhibitor type 2 lacking the
            first Kunitz-type serine proteinase inhibitor domain is a
            predominant product in mouse but not in human
  JOURNAL   Biochem Biophys Res Commun 255 (3), 740-748 (1999)
   PUBMED   10049781
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB196296.1, AI227451.1, AK007792.1 and BQ174562.1.
            
            On Mar 9, 2007 this sequence version replaced NM_011464.1.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BI415743.1, BG965694.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-466               CB196296.1         63-528
            467-822             AI227451.1         103-458
            823-1380            AK007792.1         322-879
            1381-1477           BQ174562.1         1-97                c
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 16.94 cM"
     gene            1..1477
                     /gene="Spint2"
                     /gene_synonym="HAI-2"
                     /note="serine protease inhibitor, Kunitz type 2"
                     /db_xref="GeneID:20733"
                     /db_xref="MGI:MGI:1338031"
     exon            1..466
                     /gene="Spint2"
                     /gene_synonym="HAI-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             361..1119
                     /gene="Spint2"
                     /gene_synonym="HAI-2"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; kunitz-type protease inhibitor 2; hepatocyte
                     growth factor activator inhibitor type 2"
                     /codon_start=1
                     /product="kunitz-type protease inhibitor 2 isoform a
                     precursor"
                     /protein_id="NP_035594.1"
                     /db_xref="CCDS:CCDS39870.1"
                     /db_xref="GeneID:20733"
                     /db_xref="MGI:MGI:1338031"
                     /translation="MAQLCELRRGRALLALVASLLLSGAQVASRELDVHESCGVSKVV
                     GKCRASIPRWWYNITDGSCQPFVYGGCEGNGNNYQSKEECLDKCAGVTENTTDDMARN
                     RNGADSSVLSVPRKQSAEDLSAEIFNYEEYCVPKAVTGPCRAAFPRWYYDTEKNSCIS
                     FIYGGCRGNKNSYLSQEACMQHCSGKQMHPFLTPGLKAVILVGLFLMVLILLLGTSMV
                     CLIRVVRRKQERALRTVWSTADDKEQLVKNTCVL"
     exon            467..637
                     /gene="Spint2"
                     /gene_synonym="HAI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            638..697
                     /gene="Spint2"
                     /gene_synonym="HAI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            698..751
                     /gene="Spint2"
                     /gene_synonym="HAI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            752..913
                     /gene="Spint2"
                     /gene_synonym="HAI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            914..952
                     /gene="Spint2"
                     /gene_synonym="HAI-2"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1460
                     /gene="Spint2"
                     /gene_synonym="HAI-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1443..1448
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spint2"
                     /gene_synonym="HAI-2"
                     /note="hexamer: AATAAA"
     polyA_site      1449
                     /gene="Spint2"
                     /gene_synonym="HAI-2"
                     /note="major polyA site"
ORIGIN      
        1 cccaccccca gcctgggtgc caccttctct tgtgtccctg cgtccgccat tcgccgtagt
       61 actcctcgga cgggtggagg ctttcacagt ctggggaaga gtcgcagagt gttgccgcgc
      121 gagtccaggg accgtgttgg gaggcaacta aggcgtcgca cgcgccaaaa gccagaaaaa
      181 agggcttccg gggacccggg cgcctcgaag tcctactccg gggcgaagcc gcacctgagc
      241 ccgcggacct gcagtgcgcg tgcgcggccg tgggctttcc cgcacctgct cgcgagaccc
      301 cacccccaca ccccaacggc cggcggctcg aggcctggcg cgcggcgctg gcagcaggtc
      361 atggcgcagc tttgtgagct gcggcggggc cgggcgctcc tcgccttggt agcttcgctg
      421 ctcctctccg gggcccaggt ggccagccga gaactagacg tccacgagtc ttgtggagtt
      481 tcgaaggtag tggggaaatg ccgggcttcc atccccaggt ggtggtacaa tatcactgat
      541 gggtcctgcc agccatttgt ttatggaggc tgtgaaggca atggcaataa ctaccagagc
      601 aaggaggagt gtcttgacaa gtgtgctggt gtcactgaga acaccactga tgacatggcc
      661 aggaacagga atggagccga ctcttctgtc ctgagtgttc ccagaaagca gagtgctgag
      721 gacctgtctg ctgagatctt caactatgaa gaatactgtg tcccgaaggc cgtcactggg
      781 ccgtgccgcg cagccttccc tcgctggtac tatgacactg aaaagaattc ctgtatcagc
      841 ttcatctacg gcgggtgcag aggcaacaag aacagttacc tctcccagga agcatgcatg
      901 cagcactgct ctggtaagca gatgcatcct ttcctgaccc cgggcctaaa agcggtgatc
      961 ctggtggggc tgttcctcat ggttctgatc ctcctcctgg gaacctctat ggtctgcctc
     1021 atccgtgtgg ttcgcagaaa gcaggagcgt gcgctgcgca ctgtttggag cactgcggat
     1081 gacaaggagc agctggtgaa gaacacctgt gtcttgtaga ggccccagtg ctcagaaccg
     1141 ggagtgacaa cttgtgctgt ttaaaaagag agcaactgct tgcgtctgag atcattaggg
     1201 gtttggtctt tgtttcccag tgctgttggg ctgcttaggg cctcctcttg accctgtcct
     1261 cccacagctc ccctgccctt ccccatcgcc tgtctcctct aacatcacta ggaaggagcc
     1321 ccattcttgt agcggtcact gtttgtttga tttatgtggg tttttttttt ttttaagtag
     1381 ataaaggttt tttaaaaaat caggattctg aaagaatgtg tttaataaag tgggcctttt
     1441 aaaataaaac ttactatgta aaaaaaaaaa aaaaaaa
//
