LOCUS       NM_016907               2275 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus serine protease inhibitor, Kunitz type 1 (Spint1),
            transcript variant 1, mRNA.
ACCESSION   NM_016907
VERSION     NM_016907.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Lin,H.H., Yu,I.S., Cheng,M.S., Chang,T.J., Lin,H.Y., Chang,Y.C.,
            Ko,C.J., Chen,P.H., Lin,S.W., Huang,T.C., Huang,S.Y., Chen,T.Y.,
            Kan,K.W., Huang,H.P. and Lee,M.S.
  TITLE     Spint1 disruption in mouse pancreas leads to glucose intolerance
            and impaired insulin production involving HEPSIN/MAFA
  JOURNAL   Nat Commun 15 (1), 10537 (2024)
   PUBMED   39627229
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2275)
  AUTHORS   Szabo,R., Kawaguchi,M., Kataoka,H. and Bugge,T.H.
  TITLE     Early-onset tufting enteropathy in HAI-2-deficient mice is
            independent of matriptase-mediated cleavage of EpCAM
  JOURNAL   Development 150 (17) (2023)
   PUBMED   37539662
REFERENCE   4  (bases 1 to 2275)
  AUTHORS   Yoo,D., Park,J., Lee,C., Song,I., Lee,Y.H., Yun,T., Lee,H.,
            Heguy,A., Han,J.Y., Dasen,J.S., Kim,H. and Baek,M.
  TITLE     Little skate genome provides insights into genetic programs
            essential for limb-based locomotion
  JOURNAL   Elife 11, e78345 (2022)
   PUBMED   36288084
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2275)
  AUTHORS   Kawaguchi,M., Yamamoto,K., Kataoka,H., Izumi,A., Yamashita,F.,
            Kiwaki,T., Nishida,T., Camerer,E. and Fukushima,T.
  TITLE     Protease-activated receptor-2 accelerates intestinal tumor
            formation through activation of nuclear factor-kappaB signaling and
            tumor angiogenesis in ApcMin/+ mice
  JOURNAL   Cancer Sci 111 (4), 1193-1202 (2020)
   PUBMED   31997435
  REMARK    GeneRIF: ese results suggest that serine proteases promote
            intestinal carcinogenesis through activation of PAR-2, and that
            HAI-1 plays a critical tumor suppressor role as an inhibitor of
            matriptase, kallikreins, and other PAR-2 activating proteases.
REFERENCE   6  (bases 1 to 2275)
  AUTHORS   Tanaka,H., Nagaike,K., Takeda,N., Itoh,H., Kohama,K., Fukushima,T.,
            Miyata,S., Uchiyama,S., Uchinokura,S., Shimomura,T., Miyazawa,K.,
            Kitamura,N., Yamada,G. and Kataoka,H.
  TITLE     Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is
            required for branching morphogenesis in the chorioallantoic
            placenta
  JOURNAL   Mol Cell Biol 25 (13), 5687-5698 (2005)
   PUBMED   15964823
  REMARK    GeneRIF: HAI-1 is required for branching morphogenesis in the
            chorioallantoic placenta.
REFERENCE   7  (bases 1 to 2275)
  AUTHORS   Bouzin,C., Clotman,F., Renauld,J.C., Lemaigre,F.P. and
            Rousseau,G.G.
  TITLE     The onecut transcription factor hepatocyte nuclear factor-6
            controls B lymphopoiesis in fetal liver
  JOURNAL   J Immunol 171 (3), 1297-1303 (2003)
   PUBMED   12874218
REFERENCE   8  (bases 1 to 2275)
  AUTHORS   Itoh,H., Kataoka,H., Meng,J.Y., Hamasuna,R., Kitamura,N. and
            Koono,M.
  TITLE     Mouse hepatocyte growth factor activator inhibitor type 1 (HAI-1)
            and type 2 (HAI-2)/placental bikunin genes and their promoters
  JOURNAL   Biochim Biophys Acta 1519 (1-2), 92-95 (2001)
   PUBMED   11406276
REFERENCE   9  (bases 1 to 2275)
  AUTHORS   Itoh,H., Kataoka,H., Tomita,M., Hamasuna,R., Nawa,Y., Kitamura,N.
            and Koono,M.
  TITLE     Upregulation of HGF activator inhibitor type 1 but not type 2 along
            with regeneration of intestinal mucosa
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 278 (4), G635-G643 (2000)
   PUBMED   10762618
REFERENCE   10 (bases 1 to 2275)
  AUTHORS   Itoh,H., Kataoka,H., Hamasuna,R., Kitamura,N. and Koono,M.
  TITLE     Hepatocyte growth factor activator inhibitor type 2 lacking the
            first Kunitz-type serine proteinase inhibitor domain is a
            predominant product in mouse but not in human
  JOURNAL   Biochem Biophys Res Commun 255 (3), 740-748 (1999)
   PUBMED   10049781
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL929318.11.
            
            On Mar 20, 2007 this sequence version replaced NM_016907.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF099018.1, SRR7652917.881855.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-113               AL929318.11        25176-25288
            114-656             AL929318.11        25517-26059
            657-784             AL929318.11        32971-33098
            785-923             AL929318.11        33326-33464
            924-1094            AL929318.11        33553-33723
            1095-1121           AL929318.11        33876-33902
            1122-1247           AL929318.11        34175-34300
            1248-1298           AL929318.11        34413-34463
            1299-1469           AL929318.11        35827-35997
            1470-1517           AL929318.11        36172-36219
            1518-2275           AL929318.11        36573-37330
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 59.97 cM"
     gene            1..2275
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /note="serine protease inhibitor, Kunitz type 1"
                     /db_xref="GeneID:20732"
                     /db_xref="MGI:MGI:1338033"
     exon            1..113
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            114..656
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..1723
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /note="Kunitz-type protease inhibitor 1; hepatocyte growth
                     factor activator inhibitor type 1"
                     /codon_start=1
                     /product="kunitz-type protease inhibitor 1 precursor"
                     /protein_id="NP_058603.2"
                     /db_xref="CCDS:CCDS16597.1"
                     /db_xref="GeneID:20732"
                     /db_xref="MGI:MGI:1338033"
                     /translation="MAGRRLARASISAVGVWLLCALGLQATEAELPSAPAELPGGAAC
                     LSRFTSGVPSFVLDTEASVSNGATFLGSPTARRGWDCVRSCCTTQNCNLALVELQPDG
                     GEDAISACFLMNCLYEQNFVCKFAPKEGFINYLTQELYRSYRELRTRGFGGSRIPRIW
                     MGIDLKVQLQKPLVLNEADNTDWHLLQGDSDVRVERKRPEEVELWGLKEGTYLFQLTR
                     TDSDQPEETANLTITVLTAKQTEDYCLASYKVGRCRGSFPRWYYDPKEQICKSFTFGG
                     CLGNKNNYLREEECMLACKDVQGISPKRHHPVCSGSCHATQFRCSNGCCIDGFLECDD
                     TPDCPDGSDEATCEKYTSGFDELQNIHFLSDKGYCAELPDTGFCKENIPRWYYNPFSE
                     RCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDVFGLRREGSIPTVGSAEVAIAVFL
                     VICIIVVLTILGYCFFKNQRKEFHSPLHHPPPTPASSTVSTTEDTEHLVYNHTTQPL"
     exon            657..784
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            785..923
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1094
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1121
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..1247
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1298
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1469
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1517
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..2275
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2258..2263
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /note="hexamer: ATTAAA"
     polyA_site      2271
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
     polyA_site      2275
                     /gene="Spint1"
                     /gene_synonym="HAI-1"
                     /note="major polyA site"
ORIGIN      
        1 agtttcacca gaaactagaa aggccgggag gaggcagcca atcttccgtg cagccagagc
       61 cgggcggaag ccgggcggga tcgtcggcgg tcccaccccg ttcacctgcg caggtcacaa
      121 gcaccctcgg aacccggagg ctcgcactgt gaaggtgacc cccgcctcgg ggaggagggc
      181 gacggcccct gggagcacga tggcgggccg ccgcctggcg cgggccagca tctctgcggt
      241 cggcgtatgg ctcctttgcg cgcttggcct ccaggccacc gaggccgagc tgccgtccgc
      301 acccgctgag cttcccgggg gagccgcctg cttgagccgc tttaccagcg gggtgccgtc
      361 ctttgtgctg gacaccgaag cctcagtcag caacggggcc accttcctgg gttccccgac
      421 ggcgcgccgc ggctgggact gcgtgcgctc ctgctgcacc actcagaact gcaacctggc
      481 gctggtggag ctgcagccag acggcggcga ggacgccatc tctgcctgct tcctcatgaa
      541 ctgcctgtac gagcagaact tcgtgtgcaa gttcgctccg aaggagggct tcatcaacta
      601 tcttactcag gagctttacc gctcctaccg cgagctgcgg actcgaggct ttggagggtc
      661 ccggatccct cgaatctgga tgggcataga cttgaaggtc cagctgcaga agcccctggt
      721 actgaacgag gcagacaaca cagactggca cctactgcaa ggtgacagtg atgtcagagt
      781 agagagaaaa cgtccggaag aagtggagct gtggggactt aaggagggga cctacctgtt
      841 ccaactgaca agaactgact cagaccaacc agaggaaaca gccaacctga ctatcactgt
      901 gctaactgcc aagcagacag aagattattg cctcgcatcc tacaaggtgg gccgctgccg
      961 gggctccttc ccacgctggt actacgaccc caaggaacag atctgcaaaa gttttacttt
     1021 tggaggttgc ctgggcaaca agaacaacta ccttcgtgag gaagagtgca tgctagcctg
     1081 caaggatgtg caaggaatct caccgaaaag gcaccatcca gtgtgctccg gcagctgcca
     1141 tgccacccag ttccgctgta gcaatggctg ctgtatcgat ggcttcctgg agtgtgatga
     1201 cacgccagac tgccctgatg gctccgacga ggccacctgt gaaaaataca ccagcggctt
     1261 tgatgagctt cagaatatcc atttcctcag tgacaaaggg tactgtgcag agctgccaga
     1321 cactgggttt tgcaaggaga acatcccacg ctggtattac aacccattca gtgaacgctg
     1381 tgcccgattc acctatggtg gttgctatgg gaacaagaac aactttgagg aggaacagca
     1441 gtgtcttgag tcctgccgtg gcatctccaa gaaggatgtg tttggtcttc ggagggaagg
     1501 ctccattccc actgtaggct ccgctgaggt agctattgct gtatttctgg tcatctgcat
     1561 catagtggtc ttaaccatcc tgggctactg tttcttcaag aaccaaagaa aggaattcca
     1621 cagtcccctg caccacccac ctcccacacc agccagctcc actgtttcca ccacagagga
     1681 cacagagcac ctggtctata atcacacaac ccagcctctc tgagcctggg actcttgcca
     1741 gtcttaccag gtcctgcttg ccaagacaga agctagaacc tggaaaaact tggggaccag
     1801 actcttccta cctctttcct gggcatactt actctgtctc agaagacaga tctctgggcc
     1861 tctcgcagga gtctcagctg cactcaggcc agttctggag aaggcccaag agtctggaag
     1921 gagcagagca gccttgagcc aggagtactg tatatagaag gacctgctta tccaagagct
     1981 ggagaaagtt ggtgttttgt gtcttgttca aagctgccca tcaccatccg atgacgctag
     2041 gaccttgagt ggtatcagag gccagaagcc acacactgcc ttccacatct gtccctccac
     2101 tctgcaacag gctgaaggat gtgtgtctct gcacacactg aaggatgtgt ctttgtagtt
     2161 tgtgctgtaa ataattgctt ttggggcccc agggtcatag ctcagtggta gagtgtgtgt
     2221 ggtcaccctg agctctctac accgaaccac aagctacatt aaattacaaa ataaa
//
