LOCUS       NM_183136                692 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus serine peptidase inhibitor, Kazal type 8 (Spink8),
            mRNA.
ACCESSION   NM_183136
VERSION     NM_183136.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Miyata,H., Castaneda,J.M., Fujihara,Y., Yu,Z., Archambeault,D.R.,
            Isotani,A., Kiyozumi,D., Kriseman,M.L., Mashiko,D., Matsumura,T.,
            Matzuk,R.M., Mori,M., Noda,T., Oji,A., Okabe,M.,
            Prunskaite-Hyyrylainen,R., Ramirez-Solis,R., Satouh,Y., Zhang,Q.,
            Ikawa,M. and Matzuk,M.M.
  TITLE     Genome engineering uncovers 54 evolutionarily conserved and
            testis-enriched genes that are not required for male fertility in
            mice
  JOURNAL   Proc Natl Acad Sci U S A 113 (28), 7704-7710 (2016)
   PUBMED   27357688
REFERENCE   2  (bases 1 to 692)
  AUTHORS   Sultana,D.A., Tomita,S., Hamada,M., Iwanaga,Y., Kitahama,Y.,
            Khang,N.V., Hirai,S., Ohigashi,I., Nitta,S., Amagai,T.,
            Takahashi,S. and Takahama,Y.
  TITLE     Gene expression profile of the third pharyngeal pouch reveals role
            of mesenchymal MafB in embryonic thymus development
  JOURNAL   Blood 113 (13), 2976-2987 (2009)
   PUBMED   19164599
REFERENCE   3  (bases 1 to 692)
  AUTHORS   Jalkanen,J., Kotimaki,M., Huhtaniemi,I. and Poutanen,M.
  TITLE     Novel epididymal protease inhibitors with Kazal or WAP family
            domain
  JOURNAL   Biochem Biophys Res Commun 349 (1), 245-254 (2006)
   PUBMED   16930550
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC048588.1, CN843460.1 and AW455991.1.
            
            On Sep 7, 2006 this sequence version replaced NM_183136.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CN836697.1, DV052899.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                BC048588.1         1-57
            58-590              CN843460.1         4-536
            591-692             AW455991.1         1-102               c
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 59.77 cM"
     gene            1..692
                     /gene="Spink8"
                     /gene_synonym="C630041L24Rik"
                     /note="serine peptidase inhibitor, Kazal type 8"
                     /db_xref="GeneID:78709"
                     /db_xref="MGI:MGI:1925959"
     exon            1..202
                     /gene="Spink8"
                     /gene_synonym="C630041L24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            203..258
                     /gene="Spink8"
                     /gene_synonym="C630041L24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            259..380
                     /gene="Spink8"
                     /gene_synonym="C630041L24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            381..423
                     /gene="Spink8"
                     /gene_synonym="C630041L24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            424..680
                     /gene="Spink8"
                     /gene_synonym="C630041L24Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      659..664
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spink8"
                     /gene_synonym="C630041L24Rik"
                     /note="hexamer: AATAAA"
     polyA_site      680
                     /gene="Spink8"
                     /gene_synonym="C630041L24Rik"
                     /note="major polyA site"
ORIGIN      
        1 agagatagag acgctgctgt ccttctgacg tctttaacac tgagctgagg ctccttcagt
       61 caggagtgtc ttccacaggt gaaagcaagc aaagccagag ggcaaataac tgcttccttc
      121 cacaggtctg tcaccatgaa ggtgatcttc tcagttgcag tccttgttct ggccagctca
      181 gtgtggactt cccttgcagt tgatttcatc cttcctatga actttcacat gaccggggag
      241 cttctacaaa agacaaaggc cttgtgcatc aagaatatac agttatgttg gatacttagc
      301 tacttcaagg tcagtgagcc tatatgtggc agcaaccaag tgacctacga gggcgagtgc
      361 catctctgct ccggaattct atatgaagac agaactgtaa ttaaagtgca tgatgggcca
      421 tgtgaacact cctcggatga gtctgaacac tgataaaagt tactgagttc tcagagaggc
      481 acgtggagca cgactgactc ttcaaagata cccagtttgg gagacagggt ctggagttca
      541 atggtagtgt gcactagggt gtacgaagcc ttagggagtg ttaaaagaga acagcacctt
      601 gagaaaaaca tcttgatatt agaagatact tggatcaaat tcattgattt ctttcttcaa
      661 taaatgattc tcagcataaa aaaaaaaaaa aa
//
