LOCUS       NM_001013797             594 bp    mRNA    linear   ROD 01-MAY-2024
DEFINITION  Mus musculus serine peptidase inhibitor, Kazal type 6 (Spink6),
            transcript variant 1, mRNA.
ACCESSION   NM_001013797 XM_484725 XM_901348 XM_913901 XM_922965
VERSION     NM_001013797.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 594)
  AUTHORS   Fischer,J., Wu,Z., Kantyka,T., Sperrhacke,M., Dimitrieva,O.,
            Koblyakova,Y., Ahrens,K., Graumann,N., Baurecht,H., Reiss,K.,
            Schroder,J.M., Proksch,E. and Meyer-Hoffert,U.
  TITLE     Characterization of Spink6 in mouse skin: the conserved inhibitor
            of kallikrein-related peptidases is reduced by barrier injury
  JOURNAL   J Invest Dermatol 134 (5), 1305-1312 (2014)
   PUBMED   24352040
  REMARK    GeneRIF: Our study indicates that Spink6 is a potent inhibitor of
            kallikrein related peptidases and involved in skin barrier
            function.
REFERENCE   3  (bases 1 to 594)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK028032.1 and BB609825.1.
            
            On Nov 4, 2017 this sequence version replaced NM_001013797.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BB609825.1, AV022346.2 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-587               AK028032.1         1-587
            588-594             BB609825.1         588-594
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 23.74 cM"
     gene            1..594
                     /gene="Spink6"
                     /gene_synonym="EG433180"
                     /note="serine peptidase inhibitor, Kazal type 6"
                     /db_xref="GeneID:433180"
                     /db_xref="MGI:MGI:3648654"
     exon            1..134
                     /gene="Spink6"
                     /gene_synonym="EG433180"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..394
                     /gene="Spink6"
                     /gene_synonym="EG433180"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; serine protease inhibitor Kazal-type 6"
                     /codon_start=1
                     /product="serine protease inhibitor Kazal-type 6 isoform 1
                     precursor"
                     /protein_id="NP_001013819.1"
                     /db_xref="CCDS:CCDS29224.1"
                     /db_xref="GeneID:433180"
                     /db_xref="MGI:MGI:3648654"
                     /translation="MKVAGVFLLLSLALLCFFSGAFSQGGQDKRGWITRSEGRFPGKG
                     VLRHRLFQINCGEFRDPKVFCTRESDPLCGSDGQTYGNKCAFCKALEKSSGKINLKHR
                     GKC"
     exon            135..157
                     /gene="Spink6"
                     /gene_synonym="EG433180"
                     /inference="alignment:Splign:2.1.0"
     exon            158..232
                     /gene="Spink6"
                     /gene_synonym="EG433180"
                     /inference="alignment:Splign:2.1.0"
     exon            233..348
                     /gene="Spink6"
                     /gene_synonym="EG433180"
                     /inference="alignment:Splign:2.1.0"
     exon            349..588
                     /gene="Spink6"
                     /gene_synonym="EG433180"
                     /inference="alignment:Splign:2.1.0"
     regulatory      568..573
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spink6"
                     /gene_synonym="EG433180"
                     /note="hexamer: AAGAAA"
     polyA_site      588
                     /gene="Spink6"
                     /gene_synonym="EG433180"
                     /note="major polyA site"
ORIGIN      
        1 gggtttctgg gaagtgtctt gacagagaac ctaagctgag caaagcagcc ttgatctgag
       61 tgagctaact ggcaccatga aagtagcagg tgtctttctg ctcctctctc tggctcttct
      121 ctgctttttc tcaggtgcct tcagtcaagg aggacaggac aaaagaggct ggatcacaag
      181 atcagagggc cgatttccag ggaaaggtgt cttaaggcat cgtctctttc aaattaattg
      241 tggtgagttc cgtgacccca aggtcttctg cactagggaa tctgacccac tctgcggctc
      301 tgatggccag acatatggga ataaatgtgc cttctgcaag gcattggaga aaagctctgg
      361 gaagatcaac ctgaagcatc gtgggaaatg ctgaattaga gctggtgttt tgtgccacgt
      421 tgcctgtctt cactcacact tatttttttt tatttctgct tttttttctc tagcaaatgc
      481 cataaattca taaataataa aacacaactt tttctccgtg tgggtcttat gtaggtcaca
      541 atgtctattt cctttgactt gtcaataaag aaaattctgc agaactgaaa aaaa
//
