LOCUS       NM_001081180            4765 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus serine peptidase inhibitor, Kazal type 5 (Spink5),
            mRNA.
ACCESSION   NM_001081180 NM_028237 XM_283487
VERSION     NM_001081180.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Petrova,E., Lopez-Gay,J.M., Fahrner,M., Leturcq,F., de
            Villartay,J.P., Barbieux,C., Gonschorek,P., Tsoi,L.C.,
            Gudjonsson,J.E., Schilling,O. and Hovnanian,A.
  TITLE     Comparative analyses of Netherton syndrome patients and Spink5
            conditional knock-out mice uncover disease-relevant pathways
  JOURNAL   Commun Biol 7 (1), 152 (2024)
   PUBMED   38316920
  REMARK    GeneRIF: Comparative analyses of Netherton syndrome patients and
            Spink5 conditional knock-out mice uncover disease-relevant
            pathways.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4765)
  AUTHORS   Chavarria-Smith,J., Chiu,C.P.C., Jackman,J.K., Yin,J., Zhang,J.,
            Hackney,J.A., Lin,W.Y., Tyagi,T., Sun,Y., Tao,J., Dunlap,D.,
            Morton,W.D., Ghodge,S.V., Maun,H.R., Li,H., Hernandez-Barry,H.,
            Loyet,K.M., Chen,E., Liu,J., Tam,C., Yaspan,B.L., Cai,H.,
            Balazs,M., Arron,J.R., Li,J., Wittwer,A.J., Pappu,R., Austin,C.D.,
            Lee,W.P., Lazarus,R.A., Sudhamsu,J., Koerber,J.T. and Yi,T.
  TITLE     Dual antibody inhibition of KLK5 and KLK7 for Netherton syndrome
            and atopic dermatitis
  JOURNAL   Sci Transl Med 14 (675), eabp9159 (2022)
   PUBMED   36516271
REFERENCE   4  (bases 1 to 4765)
  AUTHORS   Jackman,J.K., Stockwell,A., Choy,D.F., Xie,M.M., Lu,P., Jia,G.,
            Li,H., Abbas,A.R., Bronson,P.G., Lin,W.Y., Chiu,C.P.C., Maun,H.R.,
            Roose-Girma,M., Tam,L., Zhang,J., Modrusan,Z., Graham,R.R.,
            Behrens,T.W., White,S.R., Naureckas,T., Ober,C., Ferreira,M.,
            Sedlacek,R., Wu,J., Lee,W.P., Lazarus,R.A., Koerber,J.T.,
            Arron,J.R., Yaspan,B.L. and Yi,T.
  TITLE     Genome-wide association study identifies kallikrein 5 in type 2
            inflammation-low asthma
  JOURNAL   J Allergy Clin Immunol 150 (4), 972-978 (2022)
   PUBMED   35487308
REFERENCE   5  (bases 1 to 4765)
  AUTHORS   Zingkou,E., Pampalakis,G. and Sotiropoulou,G.
  TITLE     Cathelicidin represents a new target for manipulation of skin
            inflammation in Netherton syndrome
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1866 (10), 165831 (2020)
   PUBMED   32442469
REFERENCE   6  (bases 1 to 4765)
  AUTHORS   Yamasaki,K., Schauber,J., Coda,A., Lin,H., Dorschner,R.A.,
            Schechter,N.M., Bonnart,C., Descargues,P., Hovnanian,A. and
            Gallo,R.L.
  TITLE     Kallikrein-mediated proteolysis regulates the antimicrobial effects
            of cathelicidins in skin
  JOURNAL   FASEB J 20 (12), 2068-2080 (2006)
   PUBMED   17012259
REFERENCE   7  (bases 1 to 4765)
  AUTHORS   Galliano,M.F., Roccasecca,R.M., Descargues,P., Micheloni,A.,
            Levy,E., Zambruno,G., D'Alessio,M. and Hovnanian,A.
  TITLE     Characterization and expression analysis of the Spink5 gene, the
            mouse ortholog of the defective gene in Netherton syndrome
  JOURNAL   Genomics 85 (4), 483-492 (2005)
   PUBMED   15780751
  REMARK    GeneRIF: Mouse Spink5/Lekti, like its human counterpart, is
            involved in the control of epithelial tissue homeostasis.
REFERENCE   8  (bases 1 to 4765)
  AUTHORS   Hewett,D.R., Simons,A.L., Mangan,N.E., Jolin,H.E., Green,S.M.,
            Fallon,P.G. and McKenzie,A.N.
  TITLE     Lethal, neonatal ichthyosis with increased proteolytic processing
            of filaggrin in a mouse model of Netherton syndrome
  JOURNAL   Hum Mol Genet 14 (2), 335-346 (2005)
   PUBMED   15590704
REFERENCE   9  (bases 1 to 4765)
  AUTHORS   Descargues,P., Deraison,C., Bonnart,C., Kreft,M., Kishibe,M.,
            Ishida-Yamamoto,A., Elias,P., Barrandon,Y., Zambruno,G.,
            Sonnenberg,A. and Hovnanian,A.
  TITLE     Spink5-deficient mice mimic Netherton syndrome through degradation
            of desmoglein 1 by epidermal protease hyperactivity
  JOURNAL   Nat Genet 37 (1), 56-65 (2005)
   PUBMED   15619623
  REMARK    GeneRIF: key regulator of epidermal protease activity
REFERENCE   10 (bases 1 to 4765)
  AUTHORS   Yang,T., Liang,D., Koch,P.J., Hohl,D., Kheradmand,F. and
            Overbeek,P.A.
  TITLE     Epidermal detachment, desmosomal dissociation, and destabilization
            of corneodesmosin in Spink5-/- mice
  JOURNAL   Genes Dev 18 (19), 2354-2358 (2004)
   PUBMED   15466487
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC138293.11.
            
            On or before Jul 19, 2018 this sequence version replaced
            NM_028237.1, XM_283487.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC118011.1, AJ426449.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-120               AC138293.11        161516-161635
            121-146             AC138293.11        162718-162743
            147-274             AC138293.11        165274-165401
            275-350             AC138293.11        167567-167642
            351-478             AC138293.11        175924-176051
            479-542             AC138293.11        179407-179470
            543-670             AC138293.11        180455-180582
            671-737             AC138293.11        181821-181887
            738-865             AC138293.11        184571-184698
            866-953             AC138293.11        186056-186143
            954-1081            AC138293.11        187633-187760
            1082-1160           AC138293.11        188939-189017
            1161-1288           AC138293.11        190437-190564
            1289-1337           AC138293.11        191154-191202
            1338-1465           AC138293.11        194891-195018
            1466-1547           AC138293.11        197058-197139
            1548-1675           AC138293.11        198076-198203
            1676-1736           AC138293.11        198786-198846
            1737-1864           AC138293.11        201470-201597
            1865-1958           AC138293.11        204646-204739
            1959-2086           AC138293.11        205904-206031
            2087-2159           AC138293.11        207457-207529
            2160-2287           AC138293.11        208184-208311
            2288-2366           AC138293.11        208475-208553
            2367-2494           AC138293.11        211117-211244
            2495-2564           AC138293.11        212198-212267
            2565-2654           AC138293.11        212963-213052
            2655-2782           AC138293.11        213159-213286
            2783-2879           AC138293.11        213864-213960
            2880-3010           AC138293.11        214643-214773
            3011-3101           AC138293.11        216976-217066
            3102-4765           AC138293.11        219099-220762
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 23.74 cM"
     gene            1..4765
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /note="serine peptidase inhibitor, Kazal type 5"
                     /db_xref="GeneID:72432"
                     /db_xref="MGI:MGI:1919682"
     exon            1..120
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..3119
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /note="serine protease inhibitor, Kazal type 5; Netherton
                     syndrome"
                     /codon_start=1
                     /product="serine protease inhibitor Kazal-type 5
                     precursor"
                     /protein_id="NP_001074649.1"
                     /db_xref="CCDS:CCDS37805.1"
                     /db_xref="GeneID:72432"
                     /db_xref="MGI:MGI:1919682"
                     /translation="MKTATVPMLLTLAFYLTQDAAGEKGNQDPCMKFQAQMKNGTLTC
                     PKGNNSSQSLNDIIFQSECILCKRALEQGAPTKIMNVKVLSRANRATDPAKLNCESFK
                     QRRKDGDFICPSDTSSVCGTDGKTYRSRCELCAENAKSQNHVDVKSEGECGSSHLETD
                     MCSDFRAYVQDGRLGCTRESDPILGPDGRTHGNRCAMCAELFLKEAKENATRNRESRI
                     RRDAEKELCKEFENQVRNGRLFCTRESDPIRGPDGKMHGNKCALCAEIFMRQFTEEKG
                     KAEKNQKDAEERAKAKMEIQKRCSEFQDRARNGTLFCTRENDPIRGLDGKTHGNLCSM
                     CQAFFKTEAEEKKAEAGSRNRRGSEESETYAKLCDEYRKARKNGQLYCTRENAPIRGP
                     DGKIHGNTCSMCQAFFIQEDKARAKVKREAAKEMCSEFRNQARNGMLMCTRENDPVVG
                     PDGKRHSNKCAMCASVFLLEEEEKKKDDKTEKVDAGKAKKEAVQELCRKYHTQLRNGP
                     LRCTRRNNPIEGLDGKMYKNACFMCWAFFQQEAKKSGAGFRPKVKREVKVDCSEYLAL
                     SKRGEIFCTRENDPVRGPDGKTHGNKCAMCKAVFKKENEERKRKEGENQRITSGESSS
                     GGNPKAKDECAQYRESMKHGQLSCTRESDPVRGVDGEHYNNKCVMCKELLQKEMEETN
                     KNSASRSNGTGSATGKDVCDQFRSQMKNGKLLCTRESDPTRGPDGAMHGNKCAMCKER
                     LEKEAAEKKKKEDEEKRNTETNKSDKEDKCHEYRSMQLDGRLICTRENDPVRDADGKM
                     HVNKCAMCQMMFEREANERKMREENSRSQPTNEAKDQCGEVHNSVEDAKPRPARSSLP
                     SIRGISKDECSEFQNLMKNEKLTCPETDDPVRGADGTFYQNKCHMCRDVLKNEAMKRS
                     GLQEKSSDIRSTKEGDPEFSSSSRDSDMCKNYRILPRMGYLCPKNLNPVCGDDGQTYS
                     NPCMLCHENLMRQTNTRIHKQGACEESSNLKTVSTGTPASEKMMQ"
     exon            121..146
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            147..274
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            275..350
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            351..478
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            479..542
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            543..670
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            671..737
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            738..865
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            866..953
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1081
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1160
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1288
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1337
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1465
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1547
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1675
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1736
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1737..1864
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1865..1958
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1959..2086
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2159
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2287
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2366
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2367..2494
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2495..2564
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2654
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2655..2782
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2783..2879
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2880..3010
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3011..3101
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3102..4765
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3375..3380
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /note="hexamer: AATAAA"
     polyA_site      3398
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
     regulatory      4409..4414
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /note="hexamer: AATAAA"
     polyA_site      4430
                     /gene="Spink5"
                     /gene_synonym="2310065D10Rik; LEKT1; LETK1; VAKT1"
                     /note="major polyA site"
ORIGIN      
        1 agtcttagag caccagctga gcaatgcaca gagcagtcct gtaaccaact tacatcatct
       61 tcagcatgaa gacagccacg gtgccaatgc ttctgaccct ggcattttat ctcacacaag
      121 atgctgctgg tgagaaaggc aatcaggacc catgcatgaa atttcaagca cagatgaaaa
      181 atggtacact gacgtgtccc aaaggcaaca attcttctca aagtctcaat gacatcattt
      241 tccaaagtga atgcatcttg tgcaaaagag cactagaaca aggagcacct accaagatca
      301 tgaatgtgaa ggttttgtca agagccaaca gggccactga cccagcaaag ttgaactgtg
      361 aaagtttcaa acaaagaaga aaagatgggg atttcatctg tccatctgac acttcttcag
      421 tttgtggcac agatgggaaa acataccgca gtagatgtga actgtgtgct gagaatgcga
      481 aaagccagaa ccatgttgac gtaaagagtg aaggtgaatg tggcagcagc cacctagaga
      541 cggatatgtg cagtgatttt cgagcctatg tgcaagatgg aagacttgga tgtacacggg
      601 aaagtgatcc aatccttggt cctgatggga ggacacatgg caataggtgt gccatgtgtg
      661 ctgagctctt tttaaaagaa gctaaagaaa atgccactcg taacagagag agcagaattc
      721 ggagagatgc cgagaaggaa ctttgcaagg aatttgaaaa ccaagtgagg aatggaagac
      781 tcttctgtac ccgggaaagt gacccaatcc gtggccctga tggcaagatg catgggaaca
      841 agtgtgccct ctgtgctgag atattcatga ggcaatttac agaagaaaaa ggtaaagcag
      901 agaaaaacca gaaagatgct gaagaaaggg ctaaagctaa aatggaaatt cagaaacgat
      961 gtagtgagtt ccaggatcgt gcaaggaatg gaacgctgtt ctgtaccaga gaaaatgatc
     1021 ctattcgtgg tttggatgga aaaacacatg gcaacttgtg ttccatgtgc caggctttct
     1081 tcaaaacaga agctgaagaa aagaaggctg aagctggaag cagaaacaga agagggtctg
     1141 aagagtcaga aacatatgca aagctatgtg atgaatatcg caaggccagg aagaatggac
     1201 aactatattg caccagagag aatgccccaa tcaggggccc tgatgggaaa atccatggca
     1261 acacatgctc catgtgtcag gctttcttta ttcaagaaga caaagcaaga gcaaaggtta
     1321 aaagagaagc tgcaaaggaa atgtgcagtg agttccggaa ccaagcaaga aatggaatgc
     1381 tcatgtgcac cagagaaaat gaccctgttg ttgggccaga tggaaaaaga cattcaaaca
     1441 aatgtgcaat gtgtgcaagt gtattcctac tagaagagga agagaagaaa aaagatgaca
     1501 aaacggagaa ggttgacgct ggaaaggcga agaaagaagc tgtacaggag ctctgtcgta
     1561 aataccatac ccagctcaga aatgggccgc tccgctgcac cagaaggaat aaccccattg
     1621 agggcctgga tgggaagatg tataaaaatg cctgcttcat gtgttgggcc ttcttccagc
     1681 aagaagccaa gaaaagtggg gctggtttca gaccaaaggt taaaagagaa gttaaggttg
     1741 actgcagtga atatctggct ctttcaaaac gtggagaaat tttctgcaca cgagaaaatg
     1801 accctgtgcg tggcccagat ggcaaaaccc atggcaacaa gtgtgccatg tgcaaggccg
     1861 tcttcaagaa agaaaatgaa gaaagaaaga ggaaagaagg ggaaaatcag agaatcacct
     1921 caggggagag ttccagtggt gggaacccga aggctaagga tgaatgtgct cagtatcggg
     1981 aaagcatgaa acatggacaa ctcagctgta ctagggagag tgaccctgta cgtggtgtcg
     2041 atggggaaca ctacaacaat aaatgtgtca tgtgtaaaga actactgcag aaagaaatgg
     2101 aggaaacaaa taaaaattct gcctcaagat cgaatggaac tggatcagca acaggaaagg
     2161 atgtatgtga tcagtttaga agccaaatga aaaatgggaa actcctctgc actcgggaaa
     2221 gtgaccctac ccggggtccg gatggcgcaa tgcatggcaa caagtgtgcc atgtgtaagg
     2281 aaagactgga aaaagaagct gctgaaaaga aaaagaaaga ggatgaagag aagagaaaca
     2341 ctgaaacaaa caagagcgat aaggaggata aatgccatga ataccggagc atgcagctag
     2401 atggaaggct gatctgcacc agagaaaatg atcccgttcg agacgcagat ggcaagatgc
     2461 atgttaacaa gtgtgctatg tgtcagatga tgtttgagcg tgaagctaat gaaagaaaaa
     2521 tgagagaaga aaactcacgc tcccagccca caaatgaagc aaaggaccag tgtggagaag
     2581 ttcataactc agtagaggat gccaaaccga ggccagccag gagttctttg ccctccatcc
     2641 gtgggatcag taaagatgag tgcagtgagt ttcaaaatct catgaagaat gaaaaactta
     2701 cctgtccaga aacagatgac ccggtgcgtg gtgctgatgg aaccttttat cagaacaagt
     2761 gccacatgtg cagagatgtc cttaaaaatg aagctatgaa aaggtctggg cttcaagaaa
     2821 agtcatcaga cattaggtct actaaagaag gtgatccaga attttccagc tcctctaggg
     2881 actctgacat gtgcaaaaac taccgaatat tacccagaat gggttatctt tgcccaaaga
     2941 atctaaaccc tgtctgtggt gatgatggcc aaacatactc caacccttgt atgctctgtc
     3001 atgaaaacct gatgcgtcag actaatacac gcatacataa acaaggagca tgtgaggaaa
     3061 gcagcaacct gaaaacagta tctactggca cacctgcatc tgaaaaaatg atgcaatgat
     3121 tggtgaaatt acagagaaaa agctaagcct cgattctgaa ctgcctgcct tcaccatctg
     3181 tgtatataaa gaattcttca aactcatctt tttactatag aaaataatgc agagctgttg
     3241 gtattggact cactggtttt cagtctttcc atctctttcc ttctagactc agtgatctga
     3301 ggatgtggag aagtctccac tcagtctatg ctctggaaat ctgatcacaa tgttgtctat
     3361 ccagctgcct gtttaataaa agtagaaact cagcagaaca tccttttggg gatttgttta
     3421 tgaccgctag ataatagaaa tatattttaa tagtagctga atatatggtg ccctttgtct
     3481 tgattaaaca cagtggtaga caagttgctg ccattgttga agaagtgggg caagatggac
     3541 tctgaggcag ccagtacatg tatgaagcta cctgttaaca tgtcagaccc ctacctagta
     3601 tttgtatttc cataaatacc atacctcaat tatataagtt ttatagttga caacatccat
     3661 tactttctca taactaatta tttacttctt tataatattt ttgtttaatt tttgagatta
     3721 taccatagct tttccatgct agagtgtggg agagactaaa gagatgaatc tacaaatata
     3781 tgtaaatctc aagaatggca atctccaaca taaatgttgt atgagtcata tatgtgtatg
     3841 tatgtatgtg tgtgcatatg tatatatata cacatatata catataattt taattttgag
     3901 tagttagcaa aacaactaga taaaactaat tctaattgtt atctagtact atatatcact
     3961 tattaagtca actaatataa atgttttatt taataaatct ttgatattgg tgtatatttt
     4021 ccactattag tacctctaat ttggaccagt cttctttcaa gtgttcattg gccccatgga
     4081 gctggaggtg gctgtagtgg ataatgtaac tataccatgg gataacgatg gaaaatctga
     4141 gaaagataca tgtactagat agggatgaca tgaaacaaaa cttgtggctt caggatctct
     4201 gctgaactcc gctactctca gagtttagtt gctgatgttc tgtggtagtg gtagtggtag
     4261 ataccaggct tacaccggaa agcacagaga atcttacata tattacatag tcactctcag
     4321 ctacagaaaa aaatgttcac agtgggactg acccatttgg atctaggaag ttaattttgt
     4381 attttctctc tgctcttttg tttttataaa taaatctcca ataagccaaa tagaggattt
     4441 tatgattgtt gtcctttgta actgaatgaa aattagtcat tctttatgct acatttttct
     4501 gtgtcatcaa gaacgaagac tggattatca ataaccattt gtcaagcatg gttgagctat
     4561 gttcctgata attccgctat gaacaggatg aattcagaga ttatctgaag ctgtcttctt
     4621 catgctcata tgtaggaaat atagaaattc tctttcatga gcttttgaga aatatgtttg
     4681 tcatgtcatt tttaatattt tgttgatgtc taatcaagaa aacctttata tttcatatta
     4741 aatacaatat gcataaaatt ccttg
//
