LOCUS       NM_183284                631 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus serine peptidase inhibitor, Kazal type 2 (Spink2),
            transcript variant 2, mRNA.
ACCESSION   NM_183284
VERSION     NM_183284.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 631)
  AUTHORS   Liu,Y., Zhang,L., Li,W., Huang,Q., Yuan,S., Li,Y., Liu,J.,
            Zhang,S., Pin,G., Song,S., Ray,P.F., Arnoult,C., Cho,C.,
            Garcia-Reyes,B., Knippschild,U., Strauss,J.F. and Zhang,Z.
  TITLE     The sperm-associated antigen 6 interactome and its role in
            spermatogenesis
  JOURNAL   Reproduction 158 (2), 181-197 (2019)
   PUBMED   31146259
REFERENCE   3  (bases 1 to 631)
  AUTHORS   Kherraf,Z.E., Christou-Kent,M., Karaouzene,T., Amiri-Yekta,A.,
            Martinez,G., Vargas,A.S., Lambert,E., Borel,C., Dorphin,B.,
            Aknin-Seifer,I., Mitchell,M.J., Metzler-Guillemain,C.,
            Escoffier,J., Nef,S., Grepillat,M., Thierry-Mieg,N., Satre,V.,
            Bailly,M., Boitrelle,F., Pernet-Gallay,K., Hennebicq,S., Faure,J.,
            Bottari,S.P., Coutton,C., Ray,P.F. and Arnoult,C.
  TITLE     SPINK2 deficiency causes infertility by inducing sperm defects in
            heterozygotes and azoospermia in homozygotes
  JOURNAL   EMBO Mol Med 9 (8), 1132-1149 (2017)
   PUBMED   28554943
REFERENCE   4  (bases 1 to 631)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 631)
  AUTHORS   Dietz,J.A., Maes,M.E., Huang,S., Yandell,B.S., Schlamp,C.L.,
            Montgomery,A.D., Allingham,R.R., Hauser,M.A. and Nickells,R.W.
  TITLE     Spink2 modulates apoptotic susceptibility and is a candidate gene
            in the Rgcs1 QTL that affects retinal ganglion cell death after
            optic nerve damage
  JOURNAL   PLoS One 9 (4), e93564 (2014)
   PUBMED   24699552
  REMARK    GeneRIF: Overexpression of different variants of Spink2 in D407
            tissue culture cells increases their susceptibility to the
            apoptosis-inducing agent staurosporine in a manner consistent with
            the differential susceptibility between the DBA/2J and BALB/cByJ
            strains
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 631)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 631)
  AUTHORS   Lee,B., Park,I., Jin,S., Choi,H., Kwon,J.T., Kim,J., Jeong,J.,
            Cho,B.N., Eddy,E.M. and Cho,C.
  TITLE     Impaired spermatogenesis and fertility in mice carrying a mutation
            in the Spink2 gene expressed predominantly in testes
  JOURNAL   J Biol Chem 286 (33), 29108-29117 (2011)
   PUBMED   21705336
  REMARK    GeneRIF: SPINK2 is required for maintaining normal spermatogenesis
            and potentially regulates serine protease-mediated apoptosis in
            male germ cells.
REFERENCE   8  (bases 1 to 631)
  AUTHORS   Nelson,P.S., Pritchard,C., Abbott,D. and Clegg,N.
  TITLE     The human (PEDB) and mouse (mPEDB) Prostate Expression Databases
  JOURNAL   Nucleic Acids Res 30 (1), 218-220 (2002)
   PUBMED   11752298
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DV068802.1, AK005709.1 and AV380723.1.
            
            On Jan 29, 2014 this sequence version replaced NM_183284.2.
            
            Transcript Variant: This variant (2) contains an alternate 5'
            terminal exon and initiates translation at an alternate start
            codon, compared to variant 1. It encodes isoform 2, which is longer
            and has a distinct N-terminus, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DV068802.1, CN838365.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                DV068802.1         21-38
            19-630              AK005709.1         2-613
            631-631             AV380723.1         252-252
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 41.53 cM"
     gene            1..631
                     /gene="Spink2"
                     /gene_synonym="1700007F22Rik; HUSI-II"
                     /note="serine peptidase inhibitor, Kazal type 2"
                     /db_xref="GeneID:69982"
                     /db_xref="MGI:MGI:1917232"
     exon            1..140
                     /gene="Spink2"
                     /gene_synonym="1700007F22Rik; HUSI-II"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..358
                     /gene="Spink2"
                     /gene_synonym="1700007F22Rik; HUSI-II"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; serine protease inhibitor Kazal-type 2;
                     acrosin-trypsin inhibitor"
                     /codon_start=1
                     /product="serine protease inhibitor Kazal-type 2 isoform 2
                     precursor"
                     /protein_id="NP_899107.1"
                     /db_xref="CCDS:CCDS39121.1"
                     /db_xref="GeneID:69982"
                     /db_xref="MGI:MGI:1917232"
                     /translation="MLRLVLLLLVTDFAASHETLDSSDSQIMKRSQFRTPDCGHFDFP
                     ACPRNLNPVCGTDMNTYSNECTLCMKIREDGSHINIIKDEPC"
     exon            141..202
                     /gene="Spink2"
                     /gene_synonym="1700007F22Rik; HUSI-II"
                     /inference="alignment:Splign:2.1.0"
     exon            203..312
                     /gene="Spink2"
                     /gene_synonym="1700007F22Rik; HUSI-II"
                     /inference="alignment:Splign:2.1.0"
     exon            313..631
                     /gene="Spink2"
                     /gene_synonym="1700007F22Rik; HUSI-II"
                     /inference="alignment:Splign:2.1.0"
     regulatory      610..615
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spink2"
                     /gene_synonym="1700007F22Rik; HUSI-II"
                     /note="hexamer: AATAAA"
     polyA_site      631
                     /gene="Spink2"
                     /gene_synonym="1700007F22Rik; HUSI-II"
                     /note="major polyA site"
ORIGIN      
        1 gcactcccgc gcggtgccag cgggtggcta cttgaccact gctctgttcg ctgcacagga
       61 gagctctccc agcagagcag gaagaagagc tttagtgatg ctgagactgg tgctgttgct
      121 cctggtcaca gactttgcag cctctcatga gactctcgac tcttccgatt ctcaaatcat
      181 gaagaggtca cagttccgaa caccagactg tggtcatttt gacttcccag catgccctag
      241 gaacctcaac cctgtgtgcg gaacggatat gaacacttac agcaatgaat gtaccctgtg
      301 catgaaaatc agggaggacg gtagccatat taatatcatc aaagacgagc catgctgatg
      361 ggcagcttgc agaagagcag acggagaact cctctcctcc ggtgctccct gctcgaatcc
      421 caccccattt tcttttcccc ccatttcttt gcatgatact tgttcacaga acttctctgg
      481 gtagaggctg gaagacagtg atgtaaagcg gtttataaac ttaaaactct tgttctgggc
      541 ttccggccta caattcagtt tagttcaact tatgacccag gcgacttttg catcgcttta
      601 tttcattgga ataaaatgag cattgaattc a
//
