LOCUS       NM_001039218             566 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus serine peptidase inhibitor, Kazal type 14 (Spink14),
            mRNA.
ACCESSION   NM_001039218 XM_484723
VERSION     NM_001039218.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 566)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC138293.11 and AV381433.2.
            
            On Aug 31, 2006 this sequence version replaced NM_001039218.1.
            
            ##Evidence-Data-START##
            CDS exon combination :: AV381433.2, BC147448.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                AC138293.11        226144-226170
            28-153              AC138293.11        226972-227097
            154-197             AC138293.11        227820-227863
            198-566             AV381433.2         305-673
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 23.74 cM"
     gene            1..566
                     /gene="Spink14"
                     /gene_synonym="EG433178; Gm5505"
                     /note="serine peptidase inhibitor, Kazal type 14"
                     /db_xref="GeneID:433178"
                     /db_xref="MGI:MGI:3646952"
     exon            1..27
                     /gene="Spink14"
                     /gene_synonym="EG433178; Gm5505"
                     /inference="alignment:Splign:2.1.0"
     exon            28..153
                     /gene="Spink14"
                     /gene_synonym="EG433178; Gm5505"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..380
                     /gene="Spink14"
                     /gene_synonym="EG433178; Gm5505"
                     /note="Kazal type serine protease inhibitor 5-like 2"
                     /codon_start=1
                     /product="serine protease inhibitor Kazal-type 14
                     precursor"
                     /protein_id="NP_001034307.2"
                     /db_xref="CCDS:CCDS37806.1"
                     /db_xref="GeneID:433178"
                     /db_xref="MGI:MGI:3646952"
                     /translation="MVKYFQCSVLFSIMLHLVILAAPGARVWWPTHGLIKIKCPYKKV
                     NLSWFNKTVDPCPDLKQPICGTNFVTYDNPCILCVESLKSGGRIRYYYNGRC"
     exon            154..197
                     /gene="Spink14"
                     /gene_synonym="EG433178; Gm5505"
                     /inference="alignment:Splign:2.1.0"
     exon            198..334
                     /gene="Spink14"
                     /gene_synonym="EG433178; Gm5505"
                     /inference="alignment:Splign:2.1.0"
     exon            335..380
                     /gene="Spink14"
                     /gene_synonym="EG433178; Gm5505"
                     /inference="alignment:Splign:2.1.0"
     exon            381..566
                     /gene="Spink14"
                     /gene_synonym="EG433178; Gm5505"
                     /inference="alignment:Splign:2.1.0"
     regulatory      541..546
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spink14"
                     /gene_synonym="EG433178; Gm5505"
                     /note="hexamer: AATAAA"
     polyA_site      560
                     /gene="Spink14"
                     /gene_synonym="EG433178; Gm5505"
                     /note="major polyA site"
ORIGIN      
        1 ccattgattt catcttctct ttctaaggtg gtatcttcag acagcatctc aggacatcat
       61 tccacggtaa caccaaaagg aaaaaaaaaa tggtcaaata tttccagtgc tcagttttgt
      121 tctccattat gctccacttg gttatcctgg ctgctccagg tgctcgagtt tggtggccta
      181 cacatggact tattaagatc aaatgtcctt ataagaaagt aaatttgagt tggttcaaca
      241 aaacagtgga cccctgccct gacctaaaac aacccatctg cggcaccaat tttgtaacct
      301 atgataatcc ctgcatcttg tgtgttgaga gcttgaaatc tgggggaaga attagatatt
      361 actacaatgg aaggtgctag attcctggta acaaagacct gttggaagct ggtggctgga
      421 tggtccttct gtgttttctt taatctataa gctattccgg cttggagata gcttacaatt
      481 ttacaattaa agcaccatct cacaggttgg aggactctgg gatcaatccc aatctctaca
      541 aataaacaaa gtttcctgaa tatctt
//
