LOCUS       NM_030061                757 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus serine peptidase inhibitor, Kazal type 12 (Spink12),
            mRNA.
ACCESSION   NM_030061
VERSION     NM_030061.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 757)
  AUTHORS   Jeong,J., Lee,B., Kim,J., Kim,J., Hong,S.H., Kim,D., Choi,S.,
            Cho,B.N. and Cho,C.
  TITLE     Expressional and functional analyses of epididymal SPINKs in mice
  JOURNAL   Gene Expr Patterns 31, 18-25 (2019)
   PUBMED   30590135
REFERENCE   3  (bases 1 to 757)
  AUTHORS   Reiss,K., Meyer-Hoffert,U., Fischer,J., Sperrhacke,M., Wu,Z.,
            Dimitrieva,O., Krenek,P., Suchanova,S., Buryova,H., Brauer,R. and
            Sedlacek,R.
  TITLE     Expression and regulation of murine SPINK12, a potential orthologue
            of human LEKTI2
  JOURNAL   Exp Dermatol 20 (11), 905-910 (2011)
   PUBMED   21899598
  REMARK    GeneRIF: spink12 is especially expressed in the epidermal areas
            with thick skin and its regulation generally responds to
            differentiation signals.
REFERENCE   4  (bases 1 to 757)
  AUTHORS   Jalkanen,J., Kotimaki,M., Huhtaniemi,I. and Poutanen,M.
  TITLE     Novel epididymal protease inhibitors with Kazal or WAP family
            domain
  JOURNAL   Biochem Biophys Res Commun 349 (1), 245-254 (2006)
   PUBMED   16930550
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK136616.1.
            
            On Feb 14, 2019 this sequence version replaced NM_030061.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK136616.1, BY721165.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-757               AK136616.1         136-892
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 23.74 cM"
     gene            1..757
                     /gene="Spink12"
                     /gene_synonym="9230117E20Rik"
                     /note="serine peptidase inhibitor, Kazal type 12"
                     /db_xref="GeneID:78242"
                     /db_xref="MGI:MGI:1925492"
     exon            1..115
                     /gene="Spink12"
                     /gene_synonym="9230117E20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..318
                     /gene="Spink12"
                     /gene_synonym="9230117E20Rik"
                     /note="serine protease inhibitor Kazal-type 12; serine
                     peptidase inhibitor, Kazal type 11"
                     /codon_start=1
                     /product="serine protease inhibitor Kazal-type 12
                     precursor"
                     /protein_id="NP_084337.2"
                     /db_xref="CCDS:CCDS29225.1"
                     /db_xref="GeneID:78242"
                     /db_xref="MGI:MGI:1925492"
                     /translation="MKPAGAFLLLISLACLFLSVDAVSQGGFQAFCSNYEKTLAPDGK
                     SCPKTHKPVCGTDGKTYQNRCAFCQTAMERSLGKLGFKHEGKC"
     exon            116..141
                     /gene="Spink12"
                     /gene_synonym="9230117E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            142..269
                     /gene="Spink12"
                     /gene_synonym="9230117E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            270..757
                     /gene="Spink12"
                     /gene_synonym="9230117E20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      442..447
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spink12"
                     /gene_synonym="9230117E20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      466
                     /gene="Spink12"
                     /gene_synonym="9230117E20Rik"
                     /note="major polyA site"
ORIGIN      
        1 atgttcaacg cccctaacat ttctgtgttc tcagacctca agattctcag cgccatgaag
       61 ccagcaggtg cctttctgct tttgatcagc ctggcctgtt tgttcctctc tgtagatgct
      121 gtgagccaag gaggctttca ggctttttgc agcaactatg agaagacact ggccccagat
      181 ggaaaatcat gcccaaagac ccacaaacct gtgtgtggca ctgatggaaa aacctaccaa
      241 aaccgctgtg cattctgcca aacagccatg gaaagaagtt tggggaaact tggtttcaaa
      301 catgaaggga aatgctgatt ctcgaacatc acaatactgg gtgtgccttt gctttgcttt
      361 ttacactaag acatgtattc cttattaccc agtttccaca gcagtgtgct tcccaccaat
      421 tattacgcat aaaacaaaat aaataaagct atgccagtca caccaatgct ttgtttaatg
      481 taatgaaagc acatctaatt aatttactta cgtagactgt tacactcttc aatatgaaag
      541 gagaaatttg aaaactaagc aggctactac cacacttacc tgacccagaa atgccctatc
      601 tgtgagcagt tcttcagtag ctatgcagag ggtctgaagg aagtaaaata ggatgctctg
      661 aagtaaacat atgtaccgga tacaatatgt tggaatagtt gtttttccta aagggtgttt
      721 tgagttgttt aaggtcagaa atcaataaaa gatggac
//
