LOCUS       NM_177829               1135 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus serine peptidase inhibitor, Kazal type 10 (Spink10),
            mRNA.
ACCESSION   NM_177829
VERSION     NM_177829.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1135)
  AUTHORS   Jalkanen,J., Kotimaki,M., Huhtaniemi,I. and Poutanen,M.
  TITLE     Novel epididymal protease inhibitors with Kazal or WAP family
            domain
  JOURNAL   Biochem Biophys Res Commun 349 (1), 245-254 (2006)
   PUBMED   16930550
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK039062.1 and AV380905.2.
            
            On Sep 20, 2006 this sequence version replaced NM_177829.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ437330.1, AK039062.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-882               AK039062.1         2-883
            883-1127            AV380905.2         419-663
            1128-1135           AV380905.2         665-672
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 35.22 cM"
     gene            1..1135
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /note="serine peptidase inhibitor, Kazal type 10"
                     /db_xref="GeneID:328971"
                     /db_xref="MGI:MGI:3584533"
     exon            1..174
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            175..235
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            236..309
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             246..734
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /codon_start=1
                     /product="serine protease inhibitor Kazal-type 10"
                     /protein_id="NP_808497.1"
                     /db_xref="CCDS:CCDS29293.1"
                     /db_xref="GeneID:328971"
                     /db_xref="MGI:MGI:3584533"
                     /translation="MKLSSLWSNAFVINVAIALYAETAFLSAPKLKIDCRPYLDSNDK
                     CTREYHPVCSTSGKTYCNKCTFCKALRLDTMSSSLLWIKITFILALVVPFYYGTTFAF
                     SKEARRQPDCDKYRTFPNQCTREWNPVCGTNGFTYSNECVFCNAKIAAKEKIDYRHFG
                     PC"
     exon            310..341
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            342..457
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            458..534
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            535..572
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            573..688
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            689..1135
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      865..870
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /note="hexamer: AATACA"
     polyA_site      884
                     /gene="Spink10"
                     /gene_synonym="A230091H23Rik"
                     /note="major polyA site"
ORIGIN      
        1 accggaaata cccggaatct atttcctgga gctggttcct tgcggctctt ccgtggtgct
       61 ggtactctgt aatcctggat gtacgctggc tcccctcttc ttcattggca aaatgaatag
      121 gtgcaataaa aatattttcc agcgaaaaac aggacccttg atttcggggt gtgggtacag
      181 gaggtcctgc attggaacac ctgctgccaa gagatgatct attgagaagt acttgatttg
      241 ctcaaatgaa gttatcctca ttatggtcca atgcctttgt catcaatgtg gccattgctt
      301 tgtatgctga aactgccttc ctatcagcac ccaaacttaa aatagactgt agaccatatc
      361 tggattctaa tgacaagtgt accagagagt atcatcctgt atgttcaact agcggaaaaa
      421 cttattgcaa taaatgtact ttctgcaagg cactgagact tgatacaatg tcttcctctc
      481 tattatggat caaaatcact ttcatcctgg ccttggtggt ccccttttac tatggaacta
      541 cttttgcatt ttccaaagaa gctcgacgtc agccagattg cgacaagtac agaacattcc
      601 caaaccagtg taccagagaa tggaacccag tctgtgggac taatggattc acttacagca
      661 acgagtgtgt tttctgcaac gcaaagatag ctgctaaaga aaaaattgat tatcggcatt
      721 ttggtccctg ctgagttttg tacaatgttc cgagtcctgt ggctatccct tttcatggtg
      781 gatttggcaa tcaagtagaa tgttctcaat caatgctgaa aattcctaga cagcaaggga
      841 aactttctgt gggtaataaa gtggaataca ggatattgaa agaatttttt caagctgtga
      901 ttgaagggtt ttatttcaat caatttatta tagtgttaaa aaattgctgt cttaattttg
      961 tgtgtgaaaa aactcagtgt ggtttgtaac catagatgtt tcattcacta aaatgttaaa
     1021 taagttttat tctaagtatt atttatctta gaggcacttg cttaagatgg ttaaggtgaa
     1081 tgaacgcgtt tagaaattgt aatttgttga tgcaaaaaaa aaaaaaaccc aaaca
//
