LOCUS       NM_146043               4476 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus spindlin 1 (Spin1), transcript variant 2, mRNA.
ACCESSION   NM_146043 XM_914092 XM_923077 XM_923081 XM_923085 XM_923088
            XM_923092 XM_923094
VERSION     NM_146043.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Dias Mirandela,M., Zoch,A., Leismann,J., Webb,S., Berrens,R.V.,
            Valsakumar,D., Kabayama,Y., Auchynnikava,T., Schito,M.,
            Chowdhury,T., MacLeod,D., Xiang,X., Zou,J., Rappsilber,J.,
            Allshire,R.C., Voigt,P., Cook,A.G., Barau,J. and O'Carroll,D.
  TITLE     Two-factor authentication underpins the precision of the piRNA
            pathway
  JOURNAL   Nature 634 (8035), 979-985 (2024)
   PUBMED   39294378
  REMARK    GeneRIF: Two-factor authentication underpins the precision of the
            piRNA pathway.
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4476)
  AUTHORS   Chai,T., Liu,J., Liu,Z. and Fan,R.
  TITLE     Exosome-transported of circ_0081069 induces SPIN1 production by
            binding to miR-195-5p to inhibit radiosensitivity in esophageal
            squamous cell carcinoma
  JOURNAL   J Biochem Mol Toxicol 38 (2), e23659 (2024)
   PUBMED   38348706
  REMARK    GeneRIF: Exosome-transported of circ_0081069 induces SPIN1
            production by binding to miR-195-5p to inhibit radiosensitivity in
            esophageal squamous cell carcinoma.
REFERENCE   4  (bases 1 to 4476)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4476)
  AUTHORS   Greschik,H., Duteil,D., Messaddeq,N., Willmann,D., Arrigoni,L.,
            Sum,M., Jung,M., Metzger,D., Manke,T., Gunther,T. and Schule,R.
  TITLE     The histone code reader Spin1 controls skeletal muscle development
  JOURNAL   Cell Death Dis 8 (11), e3173 (2017)
   PUBMED   29168801
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4476)
  AUTHORS   Yuan,H., Zhang,P., Qin,L., Chen,L., Shi,S., Lu,Y., Yan,F., Bai,C.,
            Nan,X., Liu,D., Li,Y., Yue,W. and Pei,X.
  TITLE     Overexpression of SPINDLIN1 induces cellular senescence,
            multinucleation and apoptosis
  JOURNAL   Gene 410 (1), 67-74 (2008)
   PUBMED   18201843
  REMARK    GeneRIF: SPINDLIN1 is an important gene for mammalian mitotic
            chromosome functions, and disrupted regulation results in abnormal
            cell division, a mechanism that may be involved in tumorigenesis
REFERENCE   7  (bases 1 to 4476)
  AUTHORS   Evsikov,A.V., Graber,J.H., Brockman,J.M., Hampl,A., Holbrook,A.E.,
            Singh,P., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     Cracking the egg: molecular dynamics and evolutionary aspects of
            the transition from the fully grown oocyte to embryo
  JOURNAL   Genes Dev 20 (19), 2713-2727 (2006)
   PUBMED   17015433
REFERENCE   8  (bases 1 to 4476)
  AUTHORS   Gao,Y., Yue,W., Zhang,P., Li,L., Xie,X., Yuan,H., Chen,L., Liu,D.,
            Yan,F. and Pei,X.
  TITLE     Spindlin1, a novel nuclear protein with a role in the
            transformation of NIH3T3 cells
  JOURNAL   Biochem Biophys Res Commun 335 (2), 343-350 (2005)
   PUBMED   16098913
  REMARK    GeneRIF: spindlin1 gene may contribute to tumorigenesis
REFERENCE   9  (bases 1 to 4476)
  AUTHORS   Oh,B., Hampl,A., Eppig,J.J., Solter,D. and Knowles,B.B.
  TITLE     SPIN, a substrate in the MAP kinase pathway in mouse oocytes
  JOURNAL   Mol Reprod Dev 50 (2), 240-249 (1998)
   PUBMED   9590541
REFERENCE   10 (bases 1 to 4476)
  AUTHORS   Oh,B., Hwang,S.Y., Solter,D. and Knowles,B.B.
  TITLE     Spindlin, a major maternal transcript expressed in the mouse during
            the transition from oocyte to embryo
  JOURNAL   Development 124 (2), 493-503 (1997)
   PUBMED   9053325
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160122.2.
            
            On Mar 28, 2023 this sequence version replaced NM_146043.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK168544.1, SRR7345562.106398.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-147               AC160122.2         82282-82428
            148-348             AC160122.2         104526-104726
            349-397             AC160122.2         109363-109411
            398-651             AC160122.2         120795-121048
            652-885             AC160122.2         125740-125973
            886-4476            AC160122.2         130400-133990
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 26.04 cM"
     gene            1..4476
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /note="spindlin 1"
                     /db_xref="GeneID:20729"
                     /db_xref="MGI:MGI:109242"
     exon            1..147
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /inference="alignment:Splign:2.1.0"
     exon            148..348
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /inference="alignment:Splign:2.1.0"
     CDS             297..1085
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /note="isoform 2 is encoded by transcript variant 2; 30000
                     Mr metaphase complex; spindlin1"
                     /codon_start=1
                     /product="spindlin-1 isoform 2"
                     /protein_id="NP_666155.1"
                     /db_xref="CCDS:CCDS26510.1"
                     /db_xref="GeneID:20729"
                     /db_xref="MGI:MGI:109242"
                     /translation="MKTPFGKTPGQRSRADAGHAGVSANMMKKRTSHKKHRTSVGPSK
                     PVSQPRRNIVGCRIQHGWREGNGPVTQWKGTVLDQVPVNPSLYLIKYDGFDCVYGLEL
                     NKDERVSALEVLPDRVATSRISDAHLADTMIGKAVEHMFETEDGSKDEWRGMVLARAP
                     VMNTWFYITYEKDPVLYMYQLLDDYKEGDLRIMPDSNDSPPAEREPGEVVDSLVGKQV
                     EYAKEDGSKRTGMVIHQVEAKPSVYFIKFDDDFHIYVYDLVKTS"
     exon            349..397
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /inference="alignment:Splign:2.1.0"
     exon            398..651
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /inference="alignment:Splign:2.1.0"
     exon            652..885
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /inference="alignment:Splign:2.1.0"
     exon            886..4476
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1120..1125
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /note="hexamer: ATTAAA"
     polyA_site      1146
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /note="major polyA site"
     regulatory      1976..1981
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /note="hexamer: AATAAA"
     polyA_site      2000
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /note="major polyA site"
     regulatory      4453..4458
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
                     /note="hexamer: AATAAA"
     polyA_site      4476
                     /gene="Spin1"
                     /gene_synonym="Spin; SSEC P"
ORIGIN      
        1 gcagtgcgcg tgcgccgtgg cggccggggg gcgggcggcg gccgcggcga ggagcggggg
       61 tggggaggcg ccgtctggct cgggctgggc cggcggggag cacgagcgaa ccacggcggc
      121 cccgtgggca gcctcggtgg ccgacagagt gcttgggatc ccgtgtgttt ttgctgaact
      181 tgtaaaggag acatctggat ggtggattaa cacgaacctg tgggcagcct catagctgag
      241 aacattggat tttcacccta gtccagcagc tctgctgctc acatagatac agatgaatga
      301 agaccccatt cgggaagaca cctggccaac ggtccagagc tgatgcaggc catgctggag
      361 tatctgcaaa catgatgaag aagaggacat ctcacaaaaa acatcggacc agtgtgggac
      421 caagcaagcc tgtgtcccaa ccccggcgga acatcgtagg ctgcaggatc cagcatggat
      481 ggagagaggg caatggccct gttacccagt ggaaggggac tgtcctggac caggtgcctg
      541 tgaatccttc cctgtatctt ataaagtacg atggatttga ctgtgtttat ggactagaac
      601 ttaataagga tgaaagagtt tctgcactgg aagtcctccc tgatagagtt gcaacatctc
      661 ggatcagcga tgcacactta gcggacacaa tgatcggcaa agcagtggag cacatgtttg
      721 agacagagga cggctctaaa gatgagtgga gggggatggt cttggcacgg gcgcctgtca
      781 tgaacacatg gttttacatc acctatgaga aagaccctgt cttgtacatg taccagctcc
      841 tcgatgacta caaagaaggc gacctccgca tcatgcctga ttccaatgat tcgcctccag
      901 cagaaaggga gccaggagaa gttgtggaca gcctggtagg caagcaagtg gaatatgcca
      961 aagaagacgg ctcgaaaagg actggcatgg tcatccacca agtagaggcc aagccctctg
     1021 tctatttcat caagtttgat gacgatttcc atatttacgt ctacgatttg gtgaaaacat
     1081 cctagatgtc atcacaaact ttgccaaatt tgtggaacta ttaaatgtat aatttgtaga
     1141 cataaagact tgattgcttt ccagtttaat gaaagcttag atgtccctgc gaacccacaa
     1201 tctccaccag cagagctggt gttgttctga atagtgcaga ctgattcgaa cacaaggcat
     1261 ctgtgagggg agtcctccct cttcaaagca tctgtgtgag ggggtcttcc ctcttacaag
     1321 aaagctgtct gttgggggtt acaagcaagg tggtaaacgt taagctagta tcatagtcat
     1381 ttaaacctgg atagattgta accattttcc cttcactctg acactgtctt attctgctgc
     1441 cacaatgcaa gcatagtttg ttatttttgt tactgccttt tttgagagat tatgtatcta
     1501 tatagctacc tgtctagatc tgcatctgtg tgcctatgta tatattatac acacacagag
     1561 gcactcaccg ctggctctcc tttccttggg gattggggtg ggggtgataa cgttttctcc
     1621 agttcaacag gagtgcttga cccaagtcag tcatatttat aatatgcctg ctctttgttt
     1681 aaaattaaat ttgggcacag gaagcacaca gagacattta ggttgtttaa aaagtcagaa
     1741 tttctgagta ccatttcagt ctggaggctt ttagctgagt ccaggtataa attagccata
     1801 aacagtcaat gacccacagt agtctttagt aaactgttga gaaatctggg taaaaggctg
     1861 ccttcaattc taagctttgt ttttttgtgg tttttgttat tatttgtttc tgttgtttac
     1921 tctttaatcg taagtacttt taaaccacga cccaggtcct tggttcacat ggaataataa
     1981 agatgtgttt ttagatggca cattggtcca tttgtacaca cctgtcatcg ggatcttagt
     2041 acatggactt aaaaggcaac actacatcaa tggtttgtat atgtggtgaa ttcctgcaag
     2101 aacagtgcat ttgtattaga aatgtaagtt ttaaggatgg ccttgttgcc ttctatcaag
     2161 ttctctggga ctttgtaatt ttgattacta ctattgatac atggttatgg tcagaaggcc
     2221 tcttctccct tcctccctca ctccctctcc tcctagcttt ccccacagag aatgttagac
     2281 cagtatggct tcttgccttg atcctgttgt ggcagtgtct gttcctgccc caggtgtgct
     2341 attcacgcag gtttattccc tccatggcct ccgggctttg catgtctatg atgtgtgtgt
     2401 cgctgggaag gtcatgcttg ccagttatgg gtggatcgca gctcttcgcc atcaagtgca
     2461 ttcaggatta ttcctgggag gttgcttaaa gctgaaatag aactgaaatg cagttggtag
     2521 actagccaaa catctgcttg ctctcacttt gttttggaag aaccatggta gcttgtcagc
     2581 tggtagaaag atggtttgta aaaaccagtt tctcacggag gttgaaccta tctgtggctg
     2641 gacagtcttg gatactgtca agcaaacacc agaggcttgg ccaagtatag ttacaattac
     2701 ctagttgggg ttcttcctga tgcaagaaat ttttcactgt tcgaggcaca tggttattgg
     2761 ctatccattt agttttcaca gaggctgacc accttacact gctgggtggt cacaactgct
     2821 caccatgtga acatagtacc tcagttctgt ccttggctgc aatcgttgtg tgctcagtgt
     2881 tttctcatag gcaccaggga caggcttctc aaggcagtat gatataccct agtctttggt
     2941 gcctctagta tatgtgacca ggtgtcgtcc tgcatgtaaa tctccgagct gagtctgact
     3001 cacagcccat gctgtcatct gtggcacaag tgggtgtgag tacgtcctgc atgcactaac
     3061 ggtgcagaac atggcatggt gactagaaag taaaactcct tttgaagtgt cccaaggact
     3121 gggtcagtta gaacctgtct gccactatct gatgacttac attgtggttt tctacaagcc
     3181 acctcagtta actttggttc cccgtggact cccaggctgg aggaggaaag cgtgaggacg
     3241 gggatgattt tgatcacgat tccatttatt gcctttacat aaggttgagg gaaggggaag
     3301 gtttggtttg tttgtctttt aattgttttt tttttctttt tttttttttt aattccccca
     3361 ccccaccctc tttcttttgg tggcttccac cacccttaat ggtgacagac tcctgggcct
     3421 gccaccttgg acttccctta ggctcacagt tcagatctgc atgcattgct tgcattgttc
     3481 tggtatctga atgttgattc ctcatttagg agttcagcat taatttccaa aattttcatg
     3541 gggcttgtgg caacacgggc cgtgaatctg tgtataaaat ttactggcct tcttcactta
     3601 cctgctctag tatcgtatcg tgtgtgcgtg cgtgtgtgac gtcaggctgc cacgtaaact
     3661 tcagagaaga accttaaagc agaccatcca tttttgcatg ctctcttcta agtagaatgt
     3721 tcaatgtaac taaaactaaa attgcatgtc aaagagacct aggttctttc tttctttctt
     3781 tctctctttc tttcagtttg cttttggttt cctgtatatt tgcttactgt gctgttctag
     3841 tggttgtagg gtaaagacga actggtgaag cagtcgtagc gccgaccgaa ggtgattcca
     3901 gttgtatatg tcacgcagca ttcgaaggga ctgtgcacgc tctaaagatc acagttgctt
     3961 ctaagccaga tttcatttct attcttgttt tatgtgccaa accccaaagt gcattgggcc
     4021 tgaatctctg aacactgtag acccattaga aggaagattg ttctgaccgt cacaaattgt
     4081 agtgcctgaa aacacttaac actgattgtc taagagatga aagtcctcca aagatgacac
     4141 aggaaaaata ctacaagtaa ttgtggtgga aatgtctgtg ttccttggga atgctgcgct
     4201 ctctgtagtt tccatcattg gtgcagttcg aacgaatgag tagagttcag aggtcttcgt
     4261 gttcacattt aaaactagat aaatgacctc attttcttga gcttgaattc atttttaatt
     4321 ttaattttat tttatacaac gtgtagacgg ttccctgttc tctgcattta gaagtataca
     4381 cagtacaact gttaattctg taagtaattt ttataattat gatgtaactc ttatccttcc
     4441 ttaaaacatt aaaataaacc ctttatgtgc aacttg
//
