LOCUS       NM_001405283            6949 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus SPHK1 interactor, AKAP domain containing (Sphkap),
            transcript variant 3, mRNA.
ACCESSION   NM_001405283 XM_006496575
VERSION     NM_001405283.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Liu,Y., Harashima,S.I., Wang,Y., Suzuki,K., Tokumoto,S., Usui,R.,
            Tatsuoka,H., Tanaka,D., Yabe,D., Harada,N., Hayashi,Y. and
            Inagaki,N.
  TITLE     Sphingosine kinase 1-interacting protein is a dual regulator of
            insulin and incretin secretion
  JOURNAL   FASEB J 33 (5), 6239-6253 (2019)
   PUBMED   30789757
  REMARK    GeneRIF: Deletion of SKIP improves glucose tolerance through
            increasing not only insulin secretion but also GIP and GLP-1
            secretions.
REFERENCE   3  (bases 1 to 6949)
  AUTHORS   Wang,Y., Harashima,S.I., Liu,Y., Usui,R. and Inagaki,N.
  TITLE     Sphingosine kinase 1-interacting protein is a novel regulator of
            glucose-stimulated insulin secretion
  JOURNAL   Sci Rep 7 (1), 779 (2017)
   PUBMED   28396589
  REMARK    GeneRIF: These results indicate that SKIP modulates GSIS by a
            pathway distinct from that of cAMP-, PDE- and sphingosine
            kinase-dependent pathways.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6949)
  AUTHORS   Araujo,D.J., Anderson,A.G., Berto,S., Runnels,W., Harper,M.,
            Ammanuel,S., Rieger,M.A., Huang,H.C., Rajkovich,K., Loerwald,K.W.,
            Dekker,J.D., Tucker,H.O., Dougherty,J.D., Gibson,J.R. and
            Konopka,G.
  TITLE     FoxP1 orchestration of ASD-relevant signaling pathways in the
            striatum
  JOURNAL   Genes Dev 29 (20), 2081-2096 (2015)
   PUBMED   26494785
REFERENCE   5  (bases 1 to 6949)
  AUTHORS   Licatalosi,D.D., Yano,M., Fak,J.J., Mele,A., Grabinski,S.E.,
            Zhang,C. and Darnell,R.B.
  TITLE     Ptbp2 represses adult-specific splicing to regulate the generation
            of neuronal precursors in the embryonic brain
  JOURNAL   Genes Dev 26 (14), 1626-1642 (2012)
   PUBMED   22802532
REFERENCE   6  (bases 1 to 6949)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
REFERENCE   7  (bases 1 to 6949)
  AUTHORS   Terami,H., Hidaka,K., Shirai,M., Narumiya,H., Kuroyanagi,T.,
            Arai,Y., Aburatani,H. and Morisaki,T.
  TITLE     Efficient capture of cardiogenesis-associated genes expressed in ES
            cells
  JOURNAL   Biochem Biophys Res Commun 355 (1), 47-53 (2007)
   PUBMED   17286962
REFERENCE   8  (bases 1 to 6949)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 6949)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
REFERENCE   10 (bases 1 to 6949)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154144.9 and AC138359.8.
            
            On Apr 21, 2022 this sequence version replaced XM_006496575.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.702288.1,
                                           SRR17784644.99198.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849377,
                                           SAMN00849379 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-153               AC154144.9         79997-80149         c
            154-232             AC154144.9         56911-56989         c
            233-332             AC154144.9         34246-34345         c
            333-440             AC138359.8         198801-198908       c
            441-500             AC138359.8         150236-150295       c
            501-635             AC138359.8         148028-148162       c
            636-891             AC138359.8         139596-139851       c
            892-4609            AC138359.8         134884-138601       c
            4610-4795           AC138359.8         126568-126753       c
            4796-4882           AC138359.8         125235-125321       c
            4883-4935           AC138359.8         123048-123100       c
            4936-5120           AC138359.8         122775-122959       c
            5121-6949           AC138359.8         114708-116536       c
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 42.73 cM"
     gene            1..6949
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /note="SPHK1 interactor, AKAP domain containing"
                     /db_xref="GeneID:77629"
                     /db_xref="MGI:MGI:1924879"
     exon            1..153
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..5264
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /note="isoform 3 is encoded by transcript variant 3;
                     A-kinase anchor protein SPHKAP; SPHK1-interactor and AKAP
                     domain-containing protein"
                     /codon_start=1
                     /product="A-kinase anchor protein SPHKAP isoform 3"
                     /protein_id="NP_001392212.1"
                     /db_xref="GeneID:77629"
                     /db_xref="MGI:MGI:1924879"
                     /translation="MSTPGFLCKAMWVVEPNSATCLSAALPVKRHCQESSNVESPLMH
                     EGSEPQQITSSAAGNLAGSITACKKVLRSNSLLESTDYWLQNQRTPCQIGFVEDESEN
                     CASVCFVNLDVNKDACITENLQQKLVNVSPDLPNLISSMNVQQPKENEIVLLSGLASG
                     NLQADFDVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLEVVQERQLHLETN
                     VLKLEDDTNCSLSSIEEDFLTASEHLEEEIEVDDCRSGLENTNVSANVLESKKPKETT
                     QEGWDYHKEKLHCALGEKHIRKHRTPSTKTEGSKENTEENTSLKSLNRLVRPSHLKSE
                     VAGNKQLATNYSYPENIKGELETSQMLFIPRDAYLSMVKKDVLSPCSVLSEQGGSHRD
                     HDVTPNPLPPVQNGEASTGEYATNLAESVMQDAFIRLSQSQPTLPQESAVSFSMRSAL
                     LPSGCCTKDMVVPRSWNELPKIVIVQSPDGSDTVPEPNVSSWPDMEFVETSGIFSADS
                     SSRPTQSALEVALACAATVIGTISSPQATERFAMEQESLVSTYAQRGTGVQQTQVPQA
                     FMAPSTTEYSFPSALCGMTQVASAVAVCGLCEKEEATCPVAPTDLLPTSGASEEISSI
                     GSLVMERSTELGKEAIAEALLREATLILARPDAYSSLGELLESVNQRIIETTSKTQTL
                     CTESVQRNELAHTLSNVILKHSVDELHQKTTMAHPTDERHPCGTLDTLMESVNQLLHN
                     VICFTFKKMNHIVTLSEHPSFDQAAGQAWVKAFACPSSQPLSNAHGTGLVIRNLVEDA
                     SPKSNKGGARPELVNNPRLQSEFSCSHRMFDSTAKSFPKEIYLKGIMGEDTRNPHHTL
                     NYDSNERRASTDLGKLTTASEGCSGFQETEDSIVPNTQEKYICATPLNNEAQVNLSLL
                     GDDLSVPAQSTLEAKQSEVYGITDFAEELAETVVSMATEIAAICLDNSNGKQPWFCAW
                     KRGNEFLTAPNGSCRSLKRKKENSSAGSTVRKHKPPRLSEIKRKADEHPELKEKLMNR
                     VMDESMNLEDIPDSVSTFANEVAAKIMNLTEFSMVDGVWQGQSCSRTRLLGGDRWNRL
                     KASSCESIPEEDSEARVFVNSLGLMSTLSQPVSRASSVSKQSSCESITDEFSRFMVKQ
                     MENEGRGFELLLDYYAGKNASSIMSSAMQQACQKNDHLNVRPSCPSKQSSTESITEEF
                     YRYMLRDIAKESKDGASSRRSSHDWTTGLLSPSTRSPLCYRQSSMPDSRSPCSRLTVN
                     APVKANSLDGFAQNCPQDSVNVQPVSRASSSGLCKSDSCLYRRSGTDQITNMLIHETW
                     ASSIEALMRKNKIIADDSEAANASPGPVSSGSPLQVEKNANRLATSKGHRGPTLLVQE
                     SVDYQRKDAVTEGNHSPVSSPGKTAPVKKPSDFDPRRETSACHNAAGLNSPRRSLCSR
                     DVPLIQIETDQKEECIGEPGPFLSQSGSLEETEGHQPEETIPDVARNEDTAPSTCQSS
                     RDSLETSGEVEVEVLKEDIPRDESRNPPSSSEESTGSWSQLANEEDIPDDTSSFLQLS
                     ERSMSNGNSSGTSSLGIMDLDIYQESIPSSPMINELVEEKEILKEQSESIKEHASGLP
                     GRAASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIPTIYFKKSQESRIEKFLD
                     VVKLVQQKSWKVGDIFHAVVQYCKLHAEQKERTPSLFDWLLELG"
     exon            154..232
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            233..332
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            333..440
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            441..500
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            501..635
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            636..891
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            892..4609
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            4610..4795
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            4796..4882
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            4883..4935
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            4936..5120
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     exon            5121..6949
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6930..6935
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /note="hexamer: AATAAA"
     polyA_site      6949
                     /gene="Sphkap"
                     /gene_synonym="4930544G21Rik; A930009L15Rik; mKIAA1678;
                     SKIP"
                     /note="major polyA site"
ORIGIN      
        1 aatctccgct gcgggtctca gtcactgctg ctccagcctg cacgtctctg tcctcgcgca
       61 gggcgctcag gcttcttagc cttcgcctgc gctgtcagct tttctctgcc tgcggtcaac
      121 catggatgtc aactcccggc tttctgtgca aagccatgtg ggtagttgaa ccaaactctg
      181 ctacctgcct ttctgctgcc ctgccggtca agagacactg ccaagaatcc agcaacgtgg
      241 aatcaccact gatgcatgaa ggttcagagc cacagcagat caccagcagt gcagcaggca
      301 acctggctgg ctccatcacg gcctgtaaaa aggttcttcg aagcaatagc ttgctggagt
      361 ccacagacta ctggctgcag aaccagagga cgccttgcca gattgggttt gtggaagatg
      421 agtcagaaaa ctgtgcttct gtctgctttg tgaacctgga tgtcaacaaa gatgcctgca
      481 tcacagagaa cctgcagcag aaactggtca atgtttcacc ggaccttcca aacctcatca
      541 gctccatgaa tgtccaacag ccgaaagaaa atgaaattgt cctcctaagt gggttagcct
      601 ctggaaacct ccaggcagat tttgatgttt cacagtgccc ttggctacca gatatctgct
      661 tggtccagtg tgcaagaggg aacagaccaa acagtaccaa ctgcatcatc tttgagatca
      721 acaagttctt aattggtctg gaagtagtgc aggagcgtca gctccacctg gagacgaacg
      781 tgttgaagct ggaggatgat acaaactgtt ctctgtcttc aattgaagaa gattttctta
      841 cagcctccga gcatttggag gaagaaatcg aggtggacga ctgcagaagt ggtttggaaa
      901 atacaaatgt gtcagctaat gttttggaaa gtaaaaagcc aaaggaaact acccaggaag
      961 gatgggatta ccacaaggaa aagttgcatt gtgctttagg ggaaaaacac attagaaaac
     1021 accgtacgcc ctccacaaaa acagaagggt caaaggaaaa tacagaagag aacacgtcct
     1081 tgaaaagtct caatcgttta gtcaggccat cacacctgaa aagtgaagtg gcggggaaca
     1141 agcaactggc cacaaactac tcctacccag aaaatatcaa aggggaacta gaaacatctc
     1201 aaatgctgtt catccctaga gatgcctatc tctccatggt taagaaagat gtactttctc
     1261 cgtgtagtgt actcagtgag cagggaggca gccacagaga ccatgacgtt actccaaacc
     1321 ctcttcctcc cgttcaaaat ggagaagcca gcaccggaga gtatgccaca aacttagcag
     1381 aatctgtaat gcaagatgcc ttcatccgat tgtctcagtc acagcccaca ctcccccagg
     1441 aatctgcagt cagtttctct atgcgaagtg ctctccttcc cagtggctgt tgcacaaaag
     1501 atatggtggt ccctcgttca tggaacgagc tacccaaaat tgtcattgtg cagagcccag
     1561 atggcagtga cacagtccct gagccaaatg tctcctcctg gcctgacatg gaatttgttg
     1621 aaacctcggg catcttttct gcagacagtt ccagcagacc cactcagagt gccctagaag
     1681 ttgcattggc ttgtgcagcc actgtgattg gaaccatttc cagtccacag gccacagaaa
     1741 gattcgccat ggagcaagaa tccctggtat ctacctatgc ccagagaggc actggggtac
     1801 agcaaactca agtgccccaa gcattcatgg caccttctac aaccgagtat tccttcccat
     1861 cagctctgtg tggcatgaca caagtggcaa gtgctgtcgc tgtctgtggc ttatgtgaaa
     1921 aagaagaggc gacatgtcct gtagctccaa ctgacctctt gcctacatct ggggcttctg
     1981 aagaaatatc ctccatcgga agtttagtga tggagaggag cacagaactg gggaaggaag
     2041 ccattgcaga ggctctgctc agagaggcta ctctgatttt agcaaggcca gatgcttaca
     2101 gcagccttgg tgagctcttg gaatctgtga accagagaat catagaaacc acttctaaaa
     2161 cccagaccct gtgcacagaa agtgttcaaa ggaatgaact ggcacacacc ttgtccaatg
     2221 tcatcctcaa gcactccgtg gatgaacttc accagaaaac tacaatggct caccccactg
     2281 atgaaaggca tccctgtgga actctggaca ccttgatgga aagtgtgaac cagctgctgc
     2341 acaatgtgat ctgcttcaca ttcaaaaaga tgaatcacat tgtaacactc agtgagcatc
     2401 cctcatttga tcaggctgct ggtcaagcct gggtaaaagc ctttgcatgc cccagcagcc
     2461 agcctcttag caatgcacac ggtactggcc ttgtcatcag gaatcttgta gaggatgcat
     2521 ctcctaagtc aaacaagggc ggagcaaggc cagaactggt caacaacccc aggttgcaat
     2581 ctgaattctc ctgcagtcat agaatgtttg attcaactgc taagtcattc cccaaggaaa
     2641 tatatctgaa agggattatg ggagaggata ccaggaaccc tcatcataca ctaaattatg
     2701 acagcaatga acgaagagcc tctacagacc taggaaaatt gacaacagca agcgagggtt
     2761 gtagtggttt ccaggaaact gaagacagca ttgttccaaa cacccaagag aaatacatct
     2821 gtgccacacc cctaaacaat gaagctcaag ttaacctatc cttattaggt gatgacctgt
     2881 ctgttcctgc tcagtctact ctagaggcaa agcagtccga ggtctatggc atcacagatt
     2941 ttgcggaaga attggcagag actgttgtct ccatggcaac tgaaattgca gcaatctgcc
     3001 ttgacaactc caatggcaaa cagccctggt tttgtgcttg gaaaagaggg aacgagtttt
     3061 taacggcgcc taacggatcc tgccgatcct tgaagaggaa gaaggaaaac tcaagcgccg
     3121 gaagcaccgt gaggaaacac aagccacctc gcctcagtga aatcaagaga aaagctgatg
     3181 agcacccaga gctgaaagag aagctgatga acagagtcat ggatgagtcc atgaacctcg
     3241 aagacatccc tgattccgtc agtacctttg ccaatgaagt ggcagccaag atcatgaacc
     3301 tcacagagtt ttccatggtg gatggggtgt ggcaaggcca gagttgttcc cggactcggc
     3361 ttctgggtgg cgataggtgg aaccggctga aggcctcaag ctgtgagagc attcctgagg
     3421 aggactctga agccagggtc tttgtaaaca gcctgggttt gatgagtacc ttaagccaac
     3481 cagttagcag ggccagctcc gtctccaaac agtccagctg tgagagcatc accgatgagt
     3541 tttccaggtt catggtgaaa cagatggaaa acgaagggag agggtttgag ttgctgctgg
     3601 attactacgc aggcaaaaat gccagcagta tcatgagctc agcgatgcag caggcatgcc
     3661 agaaaaacga ccacctcaac gtgagaccga gctgcccctc taagcaatcc agtacagaga
     3721 gcataacaga ggagttctat aggtacatgt taagggacat cgcgaaagaa agcaaagatg
     3781 gtgcctcctc cagacgtagc agccatgatt ggaccacggg cctgctgtct ccttctacac
     3841 gatcccctct gtgttacaga cagtcatcta tgcctgacag caggtcgcca tgctccagac
     3901 taacagtaaa tgcgcctgtc aaagccaact ccttagatgg ctttgcccaa aactgccctc
     3961 aagattctgt aaatgtacag ccagtcagta gggcttcctc atctggcctc tgcaaatcag
     4021 attcctgctt gtatcgcaga agtgggactg accagatcac aaacatgcta attcatgaaa
     4081 catgggccag ctccattgag gctctcatga gaaagaacaa aatcattgct gatgatagcg
     4141 aggcagctaa tgccagtcct ggccctgttt ccagtggttc tcctttgcaa gtagagaaga
     4201 atgccaacag attagccacc agcaaaggac acagggggcc aactctgctt gtacaggaat
     4261 ctgttgatta ccagagaaaa gatgctgtta ctgaaggcaa tcattcccca gtgtcatctc
     4321 caggcaaaac agctcctgtt aaaaaaccca gtgattttga tcctagaaga gaaacctctg
     4381 catgccacaa tgctgctggt ctcaacagcc ctaggcggtc actctgctca agggatgtgc
     4441 ctttgattca gatagagaca gatcagaaag aagagtgcat tggagaacct ggacctttcc
     4501 tttcccaaag tggctcccta gaggaaacag aaggccacca acctgaagaa accatcccag
     4561 atgtggccag aaatgaagac acagctccga gcacctgtca gagctctcgt gacagccttg
     4621 agaccagtgg ggaagtagaa gtggaggtct tgaaagaaga cataccccga gatgagtccc
     4681 ggaaccctcc tagcagcagc gaggagagta caggcagctg gtcccagctg gccaatgagg
     4741 aggacatccc agacgacaca agcagctttc tgcagctcag cgagcggtcc atgagcaatg
     4801 gcaacagtag tggcactagc agtcttggca ttatggacct ggacatttat caggaaagca
     4861 taccgtcttc tcccatgatt aatgaattag tagaagaaaa ggagattctt aaagaacaat
     4921 cagaaagcat aaaggaacat gcctctggac tgccagggag agctgccagc ccccagagga
     4981 gcctactggt gatcaacttt gacctggagc cagagtgtcc tgatgctgag cttcgagcca
     5041 ctctgcagtg gatagctgcc tccgaactgg ggatcccaac aatctacttt aagaaatctc
     5101 aggaaagcag aattgaaaag tttctagatg ttgtgaagct tgttcagcag aagtcctgga
     5161 aggtgggaga tatatttcac gcggttgtcc agtactgcaa actgcatgca gagcagaagg
     5221 agaggactcc gagtctcttt gactggcttc tggaactagg ataatcagga gtacctctca
     5281 aggatttccc tcctctatcc cagcttaggt cacagtggtt tgcgctaaat gctctgaaac
     5341 tttctcaatg cacagcatcc cattagcaga actacttaaa aatctgctcc agtaccatgt
     5401 acaaggaatg aattggaaat atagtctgta ctaatcaata tcaccatggg catgcccaaa
     5461 gggcttgtat gagaatgatt tcaaaaagag atacttcagt gaagcccaaa catgaaagtt
     5521 tataacaaac ccagcctcca ggaaagctac actcacatat acatcacaat agagagtgag
     5581 ctatatctcc agatatatga atcaaataga aacaaagtca ttgggtcaca gtaattctat
     5641 aaattatgcg aaaaccttca ttgtactttg cttaatggat acaggggttg tccatctaag
     5701 gaaatactta ggatataata agtttccatg tctttttgtg aaagatttcc actcacagac
     5761 aagtaacaca tgcaaaagtg ctatcaaaat ccatgtgagg tgatttcttc tatttgcaga
     5821 ttccaataag tattgcaccc cttcccaaat gtccctgagc ccctcaaaaa ctgatgtcat
     5881 tcagtgggga aaaggaggct caaggttggc tttttccatc gctacattta ttgcttggat
     5941 aaaattatta tgggattcct gcctaaagac aagcttttaa acataaacag taaaataaat
     6001 aatagccttt tgaggaaaaa tatgcttgtg tattgaatta tataagaaaa actatttgtt
     6061 ttattgtaaa ttatgaaaag actattattg agttgtttta gagcttcata aactgtgaag
     6121 agattgttgt cttaactttt agagtgtcat gaaccattgg tgactgattg tactatgtac
     6181 gtgttaacat ctgtatagtg gacatgtttc cccaaagatc tttatagtgc tggattcagg
     6241 gtaatggcct tgtgcttgaa aaggccagct agggatgcgg actttctggc tgggtgacat
     6301 tgacactaaa ttagagaaat agaagccttt aacctttttt ttattgttta tttatagcag
     6361 agatgtactc ttttgaaaac tcagagtata tatgacacta aaagtcttct ttccctttca
     6421 actaactatg tgacatctac ctttattttc tcattcagaa agatgtttag tatgtatctt
     6481 ctgaaatatt atgctttcac tctgttttta aagcttatag aatggcttta aatgtgagcc
     6541 aagccccaga ctatcaatgt caatcttgct gggctattaa tcacatccta agaccattaa
     6601 aaaaaaagct tcctggcaag ccacaacctc cttaccccat ggtagaaatc tttttcagac
     6661 ctcagtgggt atttatcatg cagatggtcc agatttaggt gtacgttcaa tctaacaaat
     6721 gacctgggct ttgaaaccta cccatcatct cagatagaac tgtgtgtgct tctttgtgct
     6781 agtacatgag aaaaaaagat ttttattgtt ttgacagtcc cagcatattg tgtgtaaact
     6841 cttgttgtgc tcgaattaat gtttacttat cctccaaagc acaaatactg gatgtaaaca
     6901 tgtgttgtac ctaatgttgc aaactaataa ataaatttct gttgcaaaa
//
