LOCUS       NM_203280               3552 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus sphingosine kinase 2 (Sphk2), transcript variant 1,
            mRNA.
ACCESSION   NM_203280
VERSION     NM_203280.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Yokoyama,S., Koo,I., Aibara,D., Tian,Y., Murray,I.A., Collins,S.L.,
            Coslo,D.M., Kono,M., Peters,J.M., Proia,R.L., Gonzalez,F.J.,
            Perdew,G.H. and Patterson,A.D.
  TITLE     Sphingosine Kinase 2 Regulates Aryl Hydrocarbon Receptor Nuclear
            Translocation and Target Gene Activation
  JOURNAL   Adv Sci (Weinh) 11 (40), e2400794 (2024)
   PUBMED   39207053
  REMARK    GeneRIF: Sphingosine Kinase 2 Regulates Aryl Hydrocarbon Receptor
            Nuclear Translocation and Target Gene Activation.
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Diaz Escarcega,R., Murambadoro,K., Valencia,R.,
            Moruno-Manchon,J.F., Furr Stimming,E.E., Jung,S.Y. and
            Tsvetkov,A.S.
  TITLE     Sphingosine kinase 2 regulates protein ubiquitination networks in
            neurons
  JOURNAL   Mol Cell Neurosci 130, 103948 (2024)
   PUBMED   38909878
  REMARK    GeneRIF: Sphingosine kinase 2 regulates protein ubiquitination
            networks in neurons.
REFERENCE   3  (bases 1 to 3552)
  AUTHORS   Green,C.D., Brown,R.D.R., Uranbileg,B., Weigel,C., Saha,S.,
            Kurano,M., Yatomi,Y. and Spiegel,S.
  TITLE     Sphingosine kinase 2 and p62 regulation are determinants of sexual
            dimorphism in hepatocellular carcinoma
  JOURNAL   Mol Metab 86, 101971 (2024)
   PUBMED   38925249
  REMARK    GeneRIF: Sphingosine kinase 2 and p62 regulation are determinants
            of sexual dimorphism in hepatocellular carcinoma.
REFERENCE   4  (bases 1 to 3552)
  AUTHORS   Muller,T., Krieg,N., Lange-Polovinkin,A.I., Wissuwa,B.,
            Graler,M.H., Dennhardt,S. and Coldewey,S.M.
  TITLE     Deletion of Sphingosine Kinase 2 Attenuates Acute Kidney Injury in
            Mice with Hemolytic-Uremic Syndrome
  JOURNAL   Int J Mol Sci 25 (14), 7683 (2024)
   PUBMED   39062926
  REMARK    GeneRIF: Deletion of Sphingosine Kinase 2 Attenuates Acute Kidney
            Injury in Mice with Hemolytic-Uremic Syndrome.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3552)
  AUTHORS   Weigel,C., Maczis,M.A., Palladino,E.N.D., Green,C.D., Maceyka,M.,
            Guo,C., Wang,X.Y., Dozmorov,M.G., Milstien,S. and Spiegel,S.
  TITLE     Sphingosine Kinase 2 in Stromal Fibroblasts Creates a Hospitable
            Tumor Microenvironment in Breast Cancer
  JOURNAL   Cancer Res 83 (4), 553-567 (2023)
   PUBMED   36541910
  REMARK    GeneRIF: Sphingosine Kinase 2 in Stromal Fibroblasts Creates a
            Hospitable Tumor Microenvironment in Breast Cancer.
REFERENCE   6  (bases 1 to 3552)
  AUTHORS   Kharel,Y., Lee,S., Snyder,A.H., Sheasley-O'neill,S.L., Morris,M.A.,
            Setiady,Y., Zhu,R., Zigler,M.A., Burcin,T.L., Ley,K., Tung,K.S.,
            Engelhard,V.H., Macdonald,T.L., Pearson-White,S. and Lynch,K.R.
  TITLE     Sphingosine kinase 2 is required for modulation of lymphocyte
            traffic by FTY720
  JOURNAL   J Biol Chem 280 (44), 36865-36872 (2005)
   PUBMED   16093248
REFERENCE   7  (bases 1 to 3552)
  AUTHORS   Igarashi,N., Okada,T., Hayashi,S., Fujita,T., Jahangeer,S. and
            Nakamura,S.
  TITLE     Sphingosine kinase 2 is a nuclear protein and inhibits DNA
            synthesis
  JOURNAL   J Biol Chem 278 (47), 46832-46839 (2003)
   PUBMED   12954646
  REMARK    GeneRIF: a functional nuclear localization signal is mapped to the
            N-terminal region of SPHK2
REFERENCE   8  (bases 1 to 3552)
  AUTHORS   Liu,H., Toman,R.E., Goparaju,S.K., Maceyka,M., Nava,V.E.,
            Sankala,H., Payne,S.G., Bektas,M., Ishii,I., Chun,J., Milstien,S.
            and Spiegel,S.
  TITLE     Sphingosine kinase type 2 is a putative BH3-only protein that
            induces apoptosis
  JOURNAL   J Biol Chem 278 (41), 40330-40336 (2003)
   PUBMED   12835323
  REMARK    GeneRIF: the apoptotic effect of SphK2 might be because of its
            putative BH3 domain
REFERENCE   9  (bases 1 to 3552)
  AUTHORS   Yoshimoto,T., Furuhata,M., Kamiya,S., Hisada,M., Miyaji,H.,
            Magami,Y., Yamamoto,K., Fujiwara,H. and Mizuguchi,J.
  TITLE     Positive modulation of IL-12 signaling by sphingosine kinase 2
            associating with the IL-12 receptor beta 1 cytoplasmic region
  JOURNAL   J Immunol 171 (3), 1352-1359 (2003)
   PUBMED   12874225
  REMARK    GeneRIF: SPHK2 associates with the interleukin (IL)-12 receptor
            beta 1 cytoplasmic region and appears to be involved in the
            positive modulation of IL-12 signaling, especially IL-12-induced
            interferon-gamma production.
REFERENCE   10 (bases 1 to 3552)
  AUTHORS   Liu,H., Sugiura,M., Nava,V.E., Edsall,L.C., Kono,K., Poulton,S.,
            Milstien,S., Kohama,T. and Spiegel,S.
  TITLE     Molecular cloning and functional characterization of a novel
            mammalian sphingosine kinase type 2 isoform
  JOURNAL   J Biol Chem 275 (26), 19513-19520 (2000)
   PUBMED   10751414
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY256088.1, AK151990.1 and
            AC167242.4.
            
            On Feb 20, 2010 this sequence version replaced NM_203280.2.
            
            Summary: This gene encodes a kinase that phosphorylates sphingosine
            into sphingosine-1-phosphate, which is involved in cell
            differentiation, motility, and apoptosis. The encoded protein plays
            a role in maintaining cellular levels of sphingosine-1-phosphate.
            The gene product also enhances apoptosis in different cell types
            and suppresses cellular proliferation. In mast cells, the encoded
            protein is necessary for influx of calcium, protein kinase C
            activation, and cytokine production and degranulation. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, Feb 2010].
            
            Transcript Variant: This variant (1) encodes the same protein as
            variants 2 and 3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053737.1, AK151990.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               BY256088.1         1-100
            101-1243            AK151990.1         1-1143
            1244-1244           AC167242.4         112431-112431       c
            1245-2748           AK151990.1         1145-2648
            2749-3552           AC167242.4         110123-110926       c
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 29.45 cM"
     gene            1..3552
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /note="sphingosine kinase 2"
                     /db_xref="GeneID:56632"
                     /db_xref="MGI:MGI:1861380"
     exon            1..302
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /inference="alignment:Splign:2.1.0"
     exon            303..373
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /inference="alignment:Splign:2.1.0"
     exon            374..842
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             437..2290
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /EC_number="2.7.1.91"
                     /codon_start=1
                     /product="sphingosine kinase 2"
                     /protein_id="NP_975009.1"
                     /db_xref="CCDS:CCDS21261.1"
                     /db_xref="GeneID:56632"
                     /db_xref="MGI:MGI:1861380"
                     /translation="MAPPPLLPVAASTPILHGEFGSYPANGPRFALTLTTQALHIQRL
                     RPKPEARPRDGLVSLDEVSGCGTLQSRSPEDTAAYFCIYTYPRGRRGGRRRATRTFRA
                     DGATTYEENRAEAQRWATALTCLLRGVPLSGDQEITPELLPRKPRLLILVNPFGGRGL
                     AWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVSGDGLLYEV
                     LNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQVVGVDLLLNCSLLLCRG
                     GSHPLDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLASL
                     HTYRGRLSYLPATTEPALPIPGHSLPRAKSELVLAPAPAPAATHSPLHRSVSDLPLPL
                     PQPALVSPGSPEPLPDLSLNGGGPELTGDWGGAGDAPLSPDPLLPSSPNALKTAQLSP
                     IAEGPPEMPASSGFLPPTHSAPEASTWGPVDHLLPPLGSPLPQDWVTIEGEFVLMLGI
                     LPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLGCPHLGY
                     AAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVHPGLATLLTGPAGQKPQA"
     exon            843..992
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1087
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1203
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..3552
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2709..2714
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /note="hexamer: AATAAA"
     polyA_site      2732
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /note="major polyA site"
     regulatory      3000..3005
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /note="hexamer: TATAAA"
     polyA_site      3019
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
     regulatory      3524..3529
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
                     /note="hexamer: AATAAA"
     polyA_site      3544
                     /gene="Sphk2"
                     /gene_synonym="Sk2; Spk2"
ORIGIN      
        1 gggtgacgtc acatgagcgc gacgcggagc tctaccacaa tttgggtttc tcgatggtat
       61 gtgggaggac cgagtaaacc gaggcttcca gaaccaaaga gaagtcagcc tgaggaaagg
      121 gctgggaccc ggagcctctc tggcctttcc ccgtccctgc tctaacactc tccaggggta
      181 aagggaccgg agaatcagag acatgatcgg agcttgctgg acgagtcgcg tggtgactct
      241 ctggccgcac gccgaccgct tctcggtggc tcgcggagga cccggtgggc tgtgtgtcgg
      301 agcctccgaa gtagctggga tcaccgtctt tcaacacttg gcctggctct gccatttaaa
      361 gttgtgatct tggaggctgg tccaggagct gaccacaagc caagagccta ggagtgcttg
      421 ggactgaacc agggtcatgg ccccaccacc actactgcca gtggctgcca gcactccaat
      481 cctgcacggc gagtttggtt cctacccggc caacggccca cggtttgccc tcaccctcac
      541 aacacaagcc ctacacatac agcgactacg cccaaagcca gaagcccggc cccgagatgg
      601 tctagtctct ctggatgagg tctcgggctg tggcaccctg cagagccgta gccccgagga
      661 cactgcagcc tacttctgca tctacaccta cccacgtggc cgtcgagggg gccggcgcag
      721 agctacgcgg accttccggg cggatggggc caccacttat gaggagaatc gtgcagaggc
      781 ccagcgctgg gccactgccc tcacgtgtct cctccgagga gtgcctctgt caggggacca
      841 ggaaatcacc cctgaattgc tgccccggaa gcccaggctg ctcatattgg tcaatccctt
      901 tggggggcgg ggcctggcct ggcagcgctg tatggaccac gtggtgccaa tgatctctga
      961 agctgggctg tccttcaacc tcatacagac agaacgacag aaccatgccc gtgagctggt
     1021 gcaggggtta agcctgagtg agtgggaagg cattgtcact gtgtctggag acgggctgct
     1081 ttacgaggtg ctgaatgggc tccttgatcg gccagactgg gaggatgccg tgcggatgcc
     1141 cattggtgtc ctcccctgtg gatcgggcaa tgcgctagct ggggcggtga accatcatgg
     1201 cgggtttgag caggttgtcg gtgttgacct gttgctcaac tgctcgcttc ttctctgccg
     1261 tggtggcagc catcctctgg acctgctctc tgtgacgcta gcctcgggat cccgctgttt
     1321 ttccttcctg tcagtggcct ggggattctt gtcagatgtg gacattcaca gtgagcgctt
     1381 cagggccctg ggcagcgctc gattcacact gggtgcagtg ctaggcctgg cctcgttgca
     1441 tacctaccgt ggacgcctct cctacctccc cgctaccaca gaaccagcct tgcccatccc
     1501 aggccacagt ctgcctcgag ccaagtcaga actagtcttg gctccagccc cagcccccgc
     1561 cgccacccac tcgcctctac atcgatctgt gtctgacctg cccctgcccc ttccccagcc
     1621 tgccttggtc tcccctggct cccctgagcc cctgcctgac ctgtccctca atggtggtgg
     1681 tccagagctg actggagact ggggaggagc tggggatgca cctctgtccc cagacccact
     1741 gctgccttca tcccccaacg ctctcaaaac agctcagctt tcacccatcg ctgaagggcc
     1801 cccagaaatg ccagcatctt cggggttcct gcctcccacc cacagtgccc cagaagcctc
     1861 tacctggggc ccagtggacc acctcctccc tcccctgggc tctccactgc cccaagactg
     1921 ggtgacaata gagggggagt ttgtactcat gttgggcatc ttgccgagcc acctctgcgc
     1981 agacctgatg gcagccccac atgcacgctt tgatgatggc gttgtgcacc tgtgttgggt
     2041 gcggagcggc atctcacggg ctgcacttct acgcattttg ctggccatgg agcatggaaa
     2101 ccacttcagc ctgggctgcc cccatctggg ctatgctgca gcacgtgcct tccgccttga
     2161 accactcacg cctcgtggcc tgctcactgt agatggggag ttagtggagt atgggccaat
     2221 acaggcgcag gtgcacccag gtctcgccac gctgctcact gggcctgcag gtcaaaagcc
     2281 acaagcctga acgagcctaa aagcatggcg agttggtgga accagcgccc cataggctaa
     2341 gatctatcat ttacaggtag aagtggggcc cgcactcaga actgtgagga gggtggagag
     2401 tggtcctgac cctcagttcc cagaggacct agaggctcga gggtggggcc tgcctttctt
     2461 gatgtccaat gatggggcct ggaatgtatg agctagcaag gcttcttcag cttattgacc
     2521 agccagggtt tcttcttgcc tactccggtg cctctacttg actggccaat cagcccttga
     2581 ggggcaggtt cccccaggtg gtccccagat ttgcactaat gttcctcccc tggccagtta
     2641 gggatgggat gttctgtgtc ttgtgtgtcc ctctccctag tctaaaaagc aattgaaaag
     2701 gtctatgcaa taaaggttgt tgcttccctc taggccttca ccccttccac ccgacatccc
     2761 tttcagttat tgtggtccta taagggtagc agcagcaccc agcttggatg cagccttgag
     2821 tcagagccag tagctgcttt gtccgccaca acagctggct cccgggttct caagatttat
     2881 tcaggattgt gttgatggag gcggttggat ggatgggttc cccaacatgc taagagcaca
     2941 cacaggaccc tccccacccc cacaagggag gtgggggaag gctagctttt tcttaaaaat
     3001 ataaatgtat ttgtctgcat catgacgttc ttcgggcacc tagctggcca gaccactggc
     3061 catgggacaa ggaggaagtc aggtgtaagt ctgagcaagg aacaggactc tgccctggca
     3121 gggtggaggt ggcctcacag tgtcccatgc tgggcctggt agcgtgaaag cacagcacgg
     3181 tagtgggaca gctcctgccg cacagccacc acctcctgcc gcaacagggc gttttccttc
     3241 tccaggaagg cagcccgcac agatatctgg ttctccttga gtcttcttgc atctctcgac
     3301 ctcttggctg cttcattgtt cttgtacctc cggctccagt acttctcatc ctgagggtgg
     3361 aggggtgaaa ggggcactga gatacaacaa aaggaactct gcatatctga ttctgtacag
     3421 cagtgaggcc taagggctat tggtctttgc tgggacagaa acccaaactt ctgttgtcta
     3481 ctgctctggt tgctaagaac aggtctctgg cggaaaggta gagaataaat agattccagg
     3541 tgcacatgtg aa
//
