LOCUS       NM_025367               2545 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus sphingosine kinase 1 (Sphk1), transcript variant 2,
            mRNA.
ACCESSION   NM_025367
VERSION     NM_025367.7
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Baek,J.S., Lee,J.H., Kim,J.H., Cho,S.S., Kim,Y.S., Yang,J.H.,
            Shin,E.J., Kang,H.G., Kim,S.J., Ahn,S.G., Park,E.Y., Baek,D.J.,
            Yim,S.K., Kang,K.W., Ki,S.H. and Kim,K.M.
  TITLE     An inducible sphingosine kinase 1 in hepatic stellate cells
            potentiates liver fibrosis
  JOURNAL   Biochem Pharmacol 229, 116520 (2024)
   PUBMED   39236934
  REMARK    GeneRIF: An inducible sphingosine kinase 1 in hepatic stellate
            cells potentiates liver fibrosis.
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Li,R., Cairns,C., Yu,T.X., Jaladanki,R., Dodson,C.M., Chung,H.K.,
            Xiao,L., Wang,J.Y. and Turner,D.J.
  TITLE     miR-495 promotes intestinal epithelial cell apoptosis through
            downregulation of Sphingosine-1-phosphate
  JOURNAL   Physiol Rep 12 (17), e70021 (2024)
   PUBMED   39261977
  REMARK    GeneRIF: miR-495 promotes intestinal epithelial cell apoptosis
            through downregulation of Sphingosine-1-phosphate.
REFERENCE   3  (bases 1 to 2545)
  AUTHORS   Piao,J., Su,Z., He,J., Zhu,T., Fan,F., Wang,X., Yang,Z., Zhan,H.
            and Luo,D.
  TITLE     SphK1 deficiency ameliorates the development of atherosclerosis by
            inhibiting the S1P/S1PR3/Rhoa/ROCK pathway
  JOURNAL   Cell Signal 121, 111252 (2024)
   PUBMED   38852936
  REMARK    GeneRIF: SphK1 deficiency ameliorates the development of
            atherosclerosis by inhibiting the S1P/S1PR3/Rhoa/ROCK pathway.
REFERENCE   4  (bases 1 to 2545)
  AUTHORS   Soldi,R., Mandinova,A., Venkataraman,K., Hla,T., Vadas,M.,
            Pitson,S., Duarte,M., Graziani,I., Kolev,V., Kacer,D., Kirov,A.,
            Maciag,T. and Prudovsky,I.
  TITLE     Sphingosine kinase 1 is a critical component of the
            copper-dependent FGF1 export pathway
  JOURNAL   Exp Cell Res 313 (15), 3308-3318 (2007)
   PUBMED   17643421
  REMARK    GeneRIF: sphingosine kinase 1 is a component of the
            copper-dependent FGF1 release pathway.
REFERENCE   5  (bases 1 to 2545)
  AUTHORS   Venkataraman,K., Thangada,S., Michaud,J., Oo,M.L., Ai,Y., Lee,Y.M.,
            Wu,M., Parikh,N.S., Khan,F., Proia,R.L. and Hla,T.
  TITLE     Extracellular export of sphingosine kinase-1a contributes to the
            vascular S1P gradient
  JOURNAL   Biochem J 397 (3), 461-471 (2006)
   PUBMED   16623665
  REMARK    GeneRIF: results suggest that export of Sphk-1a isoform occurs
            under physiological conditions and may contribute to the
            establishment of the vascular S1P gradient.
REFERENCE   6  (bases 1 to 2545)
  AUTHORS   Kihara,A., Anada,Y. and Igarashi,Y.
  TITLE     Mouse sphingosine kinase isoforms SPHK1a and SPHK1b differ in
            enzymatic traits including stability, localization, modification,
            and oligomerization
  JOURNAL   J Biol Chem 281 (7), 4532-4539 (2006)
   PUBMED   16368679
  REMARK    GeneRIF: SPHK1a and SPHK1b differ in enzymatic traits including
            stability, localization, modification, and oligomerization.
REFERENCE   7  (bases 1 to 2545)
  AUTHORS   Shu,X., Wu,W., Mosteller,R.D. and Broek,D.
  TITLE     Sphingosine kinase mediates vascular endothelial growth
            factor-induced activation of ras and mitogen-activated protein
            kinases
  JOURNAL   Mol Cell Biol 22 (22), 7758-7768 (2002)
   PUBMED   12391145
  REMARK    GeneRIF: Data describe a novel mechanism by which vascular
            endothelial growth factor induces DNA synthesis in a pathway where
            sphingosine kinase mediates signaling from protein kinase C to Ras
            in a manner independent of Ras-guanine nucleotide exchange factor.
REFERENCE   8  (bases 1 to 2545)
  AUTHORS   Liu,H., Sugiura,M., Nava,V.E., Edsall,L.C., Kono,K., Poulton,S.,
            Milstien,S., Kohama,T. and Spiegel,S.
  TITLE     Molecular cloning and functional characterization of a novel
            mammalian sphingosine kinase type 2 isoform
  JOURNAL   J Biol Chem 275 (26), 19513-19520 (2000)
   PUBMED   10751414
REFERENCE   9  (bases 1 to 2545)
  AUTHORS   Nava,V.E., Lacana,E., Poulton,S., Liu,H., Sugiura,M., Kono,K.,
            Milstien,S., Kohama,T. and Spiegel,S.
  TITLE     Functional characterization of human sphingosine kinase-1
  JOURNAL   FEBS Lett 473 (1), 81-84 (2000)
   PUBMED   10802064
REFERENCE   10 (bases 1 to 2545)
  AUTHORS   Kohama,T., Olivera,A., Edsall,L., Nagiec,M.M., Dickson,R. and
            Spiegel,S.
  TITLE     Molecular cloning and functional characterization of murine
            sphingosine kinase
  JOURNAL   J Biol Chem 273 (37), 23722-23728 (1998)
   PUBMED   9726979
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL645851.8.
            
            On Sep 3, 2019 this sequence version replaced NM_025367.6.
            
            Summary: This gene encodes a kinase that phosphorylates sphingosine
            into sphingosine-1-phosphate, which is involved in cell
            differentiation, motility, and apoptosis. The encoded protein plays
            a role in maintaining cellular levels of sphingosine-1-phosphate.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Feb 2010].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, and
            initiates translation at an alternate start codon, compared to
            variant 1. The encoded isoform (2) has a distinct N-terminus and is
            shorter than isoform 1. Variants 2, 3 and 4 encode the same protein
            (isoform 2). This isoform is also known as Sphk1a2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10662772.2262276.1,
                                           SRR7345562.2412611.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-918               AL645851.8         70061-70978
            919-1122            AL645851.8         71580-71783
            1123-1275           AL645851.8         72459-72611
            1276-1370           AL645851.8         72690-72784
            1371-1486           AL645851.8         72862-72977
            1487-2545           AL645851.8         73222-74280
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 81.42 cM"
     gene            1..2545
                     /gene="Sphk1"
                     /gene_synonym="1110006G24Rik; Sk1; Spk1"
                     /note="sphingosine kinase 1"
                     /db_xref="GeneID:20698"
                     /db_xref="MGI:MGI:1316649"
     exon            1..918
                     /gene="Sphk1"
                     /gene_synonym="1110006G24Rik; Sk1; Spk1"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1122
                     /gene="Sphk1"
                     /gene_synonym="1110006G24Rik; Sk1; Spk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             1113..2261
                     /gene="Sphk1"
                     /gene_synonym="1110006G24Rik; Sk1; Spk1"
                     /EC_number="2.7.1.91"
                     /note="isoform 2 is encoded by transcript variant 2;
                     acetyltransferase SPHK1"
                     /codon_start=1
                     /product="sphingosine kinase 1 isoform 2"
                     /protein_id="NP_079643.2"
                     /db_xref="CCDS:CCDS25668.1"
                     /db_xref="GeneID:20698"
                     /db_xref="MGI:MGI:1316649"
                     /translation="MEPVECPRGLLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEE
                     AEITFKLILTERKNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWETAIQ
                     KPLCSLPGGSGNALAASVNHYAGYEQVTNEDLLINCTLLLCRRRLSPMNLLSLHTASG
                     LRLYSVLSLSWGFVADVDLESEKYRRLGEIRFTVGTFFRLASLRIYQGQLAYLPVGTV
                     ASKRPASTLVQKGPVDTHLVPLEEPVPSHWTVVPEQDFVLVLVLLHTHLSSELFAAPM
                     GRCEAGVMHLFYVRAGVSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAFRLEPRSQR
                     GVFSVDGELMVCEAVQGQVHPNYLWMVCGSRDAPSGRDSRRGPPPEEP"
     exon            1123..1275
                     /gene="Sphk1"
                     /gene_synonym="1110006G24Rik; Sk1; Spk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1370
                     /gene="Sphk1"
                     /gene_synonym="1110006G24Rik; Sk1; Spk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1486
                     /gene="Sphk1"
                     /gene_synonym="1110006G24Rik; Sk1; Spk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1487..2545
                     /gene="Sphk1"
                     /gene_synonym="1110006G24Rik; Sk1; Spk1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2527..2532
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sphk1"
                     /gene_synonym="1110006G24Rik; Sk1; Spk1"
                     /note="hexamer: AATAAA"
     polyA_site      2545
                     /gene="Sphk1"
                     /gene_synonym="1110006G24Rik; Sk1; Spk1"
                     /note="major polyA site"
ORIGIN      
        1 gtccacactg ctgggttcct ccagtcggtg aggatcgtgg atcccgatca tccgcggcag
       61 gcagcatctc aggccttgat agtgttccct ggggggaaag actccacctt tggaggttgc
      121 tgacgaggta ctctcatctc gacttcgtcc ccaaaccggt tcagctttct cctgaaaggc
      181 gtgccagact gcttcgggtt cggcgtctga gtgagccagc ggctgcatct ggaagctaaa
      241 agtaaaggca ccgcggagga gcaggggagg aagagaagtt cgcgggtggc gggtgacccg
      301 accctggccc agagtcagga cagcactagg gtctggatgt ggaattcagg ctaggtctaa
      361 gcgacgccaa agaggcgctg catccctact gtgaacagca gtttcgaggt tcagtaagcg
      421 cagacccacg cgtacccgaa gcccgctcgg atgctgggcg ccggcatgtg ggctgcgccc
      481 tcgctttcgc gctccgcgga tgcgctgctt caggccgggc tcagaacccc acctcttgcg
      541 gacttcctaa gctgttcctc cagcaccggg cgcacaatcg tatccgggga tcctgcccct
      601 catctgcgcg tggccgagcc caggctggcg gcgactgtgt tcggggaggt gcagtgagat
      661 cccgggcggg tgggggacca gagctgcgcg caactctggt tccaccccga gagcacaagg
      721 aacgcggggc gtggccaggc cggcccagag gctcagcgcc ctccccgggc cgccgtgaaa
      781 ttgagcaaaa agtttgagac cggagggggg cgatcccttg gagtcggtgt cagcccagtg
      841 cttcagttgt ccctctgtcg cgggtctgag tcggagagga gctgctttcc gccactggcc
      901 gcccgtggga cagattggat ctggccgccc cggggaatga cgagggcgct cacacagccc
      961 agggaggtgg agagggcgag ccccacggcc agtcgccaga caccctcctg ggcaacaccg
     1021 ataagaagct gaacgcagga gccgccgtta cctctagcag cgccggggca gcaccggtgg
     1081 ccccttgtca gcgggagccc cgggacctgg ctatggaacc agtagaatgc cctcgaggac
     1141 tgctcccacg gccatgcaga gtgctggtgc tgctgaaccc ccagggtggc aagggcaagg
     1201 ctctgcagct cttccagagc cgtgtgcagc ccttcctgga ggaggcagag ataaccttta
     1261 aactgatact caccgaacgg aagaaccatg ccagggagct ggtgtgtgca gaggagttgg
     1321 gtcactggga cgccctggca gtcatgtccg gtgatggtct gatgcatgag gtggtgaatg
     1381 ggctaatgga acggccagac tgggagactg ccatccagaa acccctgtgt agcctccctg
     1441 gaggctccgg caatgcgctg gcagcttctg tgaaccacta tgctgggtac gagcaggtga
     1501 ctaatgaaga cctgctcatc aactgcacac tgctgttgtg ccgccggcgc ctgtcaccca
     1561 tgaacctgct gtccctgcac actgcttctg ggctgcggct ctattctgtg ctcagtctgt
     1621 cctggggctt tgttgctgac gtggacctcg agagtgagaa gtacaggcgc ttgggggaga
     1681 ttcgtttcac agtgggcacc ttctttcgcc tagcaagcct gcgcatctac caaggccaac
     1741 tggcctacct tcctgtagga actgtggcct ctaagagacc cgcctctaca ctggtgcaga
     1801 agggccccgt cgacacacac cttgttcctc tggaggagcc agtgccttct cattggactg
     1861 tggtaccaga acaggacttc gtcctggtgc tggtgctgct acacacccac ctgagctccg
     1921 agctgtttgc agcacccatg ggccgctgtg aggctggtgt tatgcatctg ttctacgtac
     1981 gtgcgggggt gtcaagggct gcgctgctgc gcctcttcct ggccatgcag aagggcaagc
     2041 atatggaact tgactgtcca tacctggttc atgtgcccgt ggttgctttc cgcctggagc
     2101 ccaggagcca gaggggcgtg ttttctgtgg atggagagct gatggtatgt gaagctgtgc
     2161 agggccaagt gcacccaaac tacctttgga tggtctgtgg cagcagagat gccccatccg
     2221 gccgggactc ccggcggggg ccacctccag aagaaccata actctgtgcc tttgtctact
     2281 ctgtctaggc tgagatggga ccctcccccg cacccacctc ctggtatggg aggttatttc
     2341 taaagttcct atggaagtgg tggggacccc tgcagagaaa gctagaaggt ggggctatga
     2401 cttggaaaga aaggctctac cttccagtta gagtaacatc cccagtagag ccctgctggc
     2461 tggaccagtt gcatatagaa gacactcccc attgctctta gggaccttcc ctgggaacca
     2521 aattcaaata aagagacttt tccaa
//
