LOCUS       NM_138584               2666 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus SPG21, maspardin (Spg21), transcript variant 1, mRNA.
ACCESSION   NM_138584
VERSION     NM_138584.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Davenport,A., Bivona,A., Latson,W., Lemanski,L.F. and Cheriyath,V.
  TITLE     Loss of Maspardin Attenuates the Growth and Maturation of Mouse
            Cortical Neurons
  JOURNAL   Neurodegener Dis 16 (3-4), 260-272 (2016)
   PUBMED   26978163
  REMARK    GeneRIF: Study showed that the loss of maspardin attenuates the
            growth, maturation, and axonal branching of cortical neurons to
            promote progressive neuronal dysfunction; impairment of sensory
            motor neurons in SPG21-/- mice; and that SPG21-/- cortical neurons
            failed to respond to EGF-induced growth and maturation, which is
            confirmed by the attenuated expression of a subset of EGFR target
            genes
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Soderblom,C., Stadler,J., Jupille,H., Blackstone,C., Shupliakov,O.
            and Hanna,M.C.
  TITLE     Targeted disruption of the Mast syndrome gene SPG21 in mice impairs
            hind limb function and alters axon branching in cultured cortical
            neurons
  JOURNAL   Neurogenetics 11 (4), 369-378 (2010)
   PUBMED   20661613
  REMARK    GeneRIF: Mast syndrome gene SPG21 in mice has a role in hind limb
            function and axon branching in cultured cortical neurons
REFERENCE   3  (bases 1 to 2666)
  AUTHORS   Hitzemann,R., Reed,C., Malmanger,B., Lawler,M., Hitzemann,B.,
            Cunningham,B., McWeeney,S., Belknap,J., Harrington,C., Buck,K.,
            Phillips,T. and Crabbe,J.
  TITLE     On the integration of alcohol-related quantitative trait loci and
            gene expression analyses
  JOURNAL   Alcohol Clin Exp Res 28 (10), 1437-1448 (2004)
   PUBMED   15597075
  REMARK    Erratum:[Alcohol Clin Exp Res. 2004 Dec;28(12):1930]
REFERENCE   4  (bases 1 to 2666)
  AUTHORS   Zeitlmann,L., Sirim,P., Kremmer,E. and Kolanus,W.
  TITLE     Cloning of ACP33 as a novel intracellular ligand of CD4
  JOURNAL   J Biol Chem 276 (12), 9123-9132 (2001)
   PUBMED   11113139
REFERENCE   5  (bases 1 to 2666)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC114645.9.
            
            On Nov 3, 2022 this sequence version replaced NM_138584.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK149930.1, SRR7345562.1491862.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-193               AC114645.9         66480-66672         c
            194-280             AC114645.9         62284-62370         c
            281-442             AC114645.9         58698-58859         c
            443-523             AC114645.9         53916-53996         c
            524-669             AC114645.9         51649-51794         c
            670-778             AC114645.9         47147-47255         c
            779-886             AC114645.9         45666-45773         c
            887-1027            AC114645.9         43052-43192         c
            1028-2666           AC114645.9         39163-40801         c
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 35.32 cM"
     gene            1..2666
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /note="SPG21, maspardin"
                     /db_xref="GeneID:27965"
                     /db_xref="MGI:MGI:106403"
     exon            1..193
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /inference="alignment:Splign:2.1.0"
     exon            194..280
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /inference="alignment:Splign:2.1.0"
     CDS             218..1144
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /note="isoform 1 is encoded by transcript variant 1;
                     maspardin; acid cluster protein 33; spastic paraplegia 21
                     autosomal recessive Mast syndrome protein homolog; spastic
                     paraplegia 21 homolog"
                     /codon_start=1
                     /product="maspardin isoform 1"
                     /protein_id="NP_613050.1"
                     /db_xref="CCDS:CCDS23293.1"
                     /db_xref="GeneID:27965"
                     /db_xref="MGI:MGI:106403"
                     /translation="MGEIKVSPDYNWFRSTVPLKKIIVDDDDSKIWSLYDAGPRSIRC
                     PLIFLPPVSGTADVFFQQILALTGWGYRVIALQYPVYWDHLEFCDGFRKLLDHLQLDK
                     VHLFGASLGGFLAQKFAEYTHKSPRVHSLILCNAFSDTSIFNQTWTANSFWLMPAFML
                     KKIVLGNFSSGPVDPMMADAIDFMVDRLESLGQSELASRLTLNCQNSYVEPHKIRDIP
                     VTIMDVFDQSALSTEAKEEMYKLYPNARRAHLKTGGNFPYLCRSAEVNLYVQIHLLQF
                     HGTKYAAIDPSVVSAEELEVQKGRLGLSQEEP"
     exon            281..442
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /inference="alignment:Splign:2.1.0"
     exon            443..523
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /inference="alignment:Splign:2.1.0"
     exon            524..669
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /inference="alignment:Splign:2.1.0"
     exon            670..778
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /inference="alignment:Splign:2.1.0"
     exon            779..886
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1027
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..2666
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1634..1639
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /note="hexamer: AATAAA"
     polyA_site      1651
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /note="major polyA site"
     regulatory      2645..2650
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
                     /note="hexamer: AATAAA"
     polyA_site      2666
                     /gene="Spg21"
                     /gene_synonym="ACP33; BM-019; D9Wsu18e; GL010; MAST"
ORIGIN      
        1 gcagtaccaa agctgggagg cggtggcctc tggctgctcg gccgcgcgcc ccctctgccc
       61 gctgctcccg cggggctcct gatctgcggc cgccggtcca gtccgcgccc cgccgactcc
      121 atcctctgcg cccaaatcgg ggcgaagatc cgtctaggct ggaaagaaag ggacgcggga
      181 gcggccacgg cagggttaac ctctgcttct gctcatcatg ggagagatca aagtctctcc
      241 agattataat tggtttagaa gtactgttcc cctgaaaaag ataatcgtgg atgacgatga
      301 cagtaagatc tggtcgctct acgacgctgg ccccagaagc atcaggtgtc ctctcatctt
      361 tcttcctcct gtcagcggaa ctgcagatgt gtttttccag cagattctgg ctttgactgg
      421 atggggttac cgggttatag ctttgcagta tccagtttat tgggaccatc ttgaattctg
      481 tgatggattc agaaaacttt tagaccactt acaactggat aaagttcatc tgtttggggc
      541 atctttggga ggctttttgg ctcaaaaatt tgctgaatac actcacaagt ctcccagggt
      601 acactccctc atcctgtgca atgccttcag tgacacgtct atcttcaacc aaacatggac
      661 tgcaaatagc ttctggttga tgccagcgtt tatgctcaag aaaatcgttt tggggaactt
      721 ttcctctggc ccagtagacc cgatgatggc tgatgccatt gatttcatgg tggacaggct
      781 ggagagtttg ggtcaaagtg aactggcttc aaggctaact ctcaactgtc agaattccta
      841 tgtggagcca cataagatcc gggacatccc cgtcaccatc atggatgtgt ttgaccagag
      901 tgcgctgtca acagaagcca aggaagaaat gtacaaactg taccctaatg cgcggagagc
      961 ccaccttaag acaggaggca attttcccta cctgtgcagg agtgcagagg tcaatcttta
     1021 tgtgcagata catctgctgc agttccatgg aaccaagtac gctgccatcg atccgtcagt
     1081 ggtcagtgcg gaggagctag aggtgcagaa aggccgcctc ggcctcagcc aggaagagcc
     1141 atagatgggg cttgagatgc cgaaccaccc tgctcttgca caatcagttc gggttcgttg
     1201 gtcgggcaca ctggtcctca cccttctgga agtcggactg attgtcaatc tcatgacagg
     1261 cgtcagctct tctaaaccag tgtgactggc ccctctccac atttgttttt gttttaaact
     1321 tttaaatttg aagaactttt aaagactccc attttaagaa ttgtgaaaat tttgctacca
     1381 aaagtcttct ccactctgtt catctcaaaa gcttgggatc atgtggcgtt ctctcttttc
     1441 ctgctttcta taactgctgc acccacctcc aggttagaag aggagcaccg gtcattttta
     1501 tgctttcttg ggcataacca gagggtcgag gctggcagcc tgctggccac tccctacaca
     1561 tcagcttttt tgctttccag ttgagcacat cattttgagt attagctgtt gtatttctag
     1621 acattacatt tggaataaaa attgaatttc agccagtggc ttctgtgatg agtgaggctg
     1681 cccctctggg ctactggcca gtgctgtgtg atgtcgggtg aacaagtcca tcactctgac
     1741 tgcagcacgg ctggctgtta cctgctgact gccgctgtag cctacgtgct cttttaatct
     1801 ttttcactaa tgggattgtt aaaaaagaga tagagtctca tttaggctag gctggccttg
     1861 aactcattat gtgtcagggc tagcttagag ctcctgatcc ccctgcctcc acctcccaag
     1921 tgctaggatt acagatgtta tcctgaaaga tcaccagatg cccgatagaa gtggttttaa
     1981 tacatatgga gaacatagta aactgtctcc agctcttgat ccaatgtaga aaacgtccag
     2041 cctgtggata gccttgtctc cctcccgcac ttttctacat cccttcctat cctggatggc
     2101 tataaagcat ttccatgtgt cctttcaaga gatatgatag gatacttgag acgctgggga
     2161 gatgagtctg tgagtaaaag tgcgtgctgg acgagcatga gcacttgaga ccgcccagtg
     2221 cccgtgcaca gtgtcaagag tgtccaggca gcctgtaacc tcagatagcc cagtgcccgt
     2281 gcacagagtc aagagtgtct aggcagcctg taacctcaga tagcccagtg ctcgtgcaca
     2341 gtgtcaagag tgtccaggca gcctgtaacc tcagatagcc cagtgcccgt gcacagtgtc
     2401 aagagtgtcc aggcagcctg taacctcaga tagcccagtg cccgtgcaca gtgtcaagag
     2461 tgtccacata gcctgtaacc tcagatagcc cagtgcccgt gcacagtgtc aagagtatcc
     2521 acacagcctg taacctcaac actagctgca gggcatccat tctctggcat ctgcccacac
     2581 atacacagat atatacaccc atctgtatac aggtatctat acagtaacaa agaggttatt
     2641 tgataataaa ggttgaaata tctgta
//
