LOCUS       NM_025721               3013 bp    mRNA    linear   ROD 11-JUN-2024
DEFINITION  Mus musculus sperm equatorial segment protein 1 (Spesp1), mRNA.
ACCESSION   NM_025721
VERSION     NM_025721.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Zhong,Y., Zhou,L., Guo,Y., Wang,F., He,F., Cheng,Y., Meng,X.,
            Xie,H., Zhang,Y. and Li,J.
  TITLE     Downregulated SPESP1-driven fibroblast senescence decreases wound
            healing in aged mice
  JOURNAL   Clin Transl Med 14 (5), e1660 (2024)
   PUBMED   38764260
  REMARK    GeneRIF: Downregulated SPESP1-driven fibroblast senescence
            decreases wound healing in aged mice.
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Ito,C., Yamatoya,K., Yoshida,K., Fujimura,L., Sugiyama,H.,
            Suganami,A., Tamura,Y., Hatano,M., Miyado,K. and Toshimori,K.
  TITLE     Deletion of Eqtn in mice reduces male fertility and sperm-egg
            adhesion
  JOURNAL   Reproduction 156 (6), 579-590 (2018)
   PUBMED   30328350
REFERENCE   3  (bases 1 to 3013)
  AUTHORS   Nakata,H., Wakayama,T., Asano,T., Nishiuchi,T. and Iseki,S.
  TITLE     Identification of sperm equatorial segment protein 1 in the
            acrosome as the primary binding target of peanut agglutinin (PNA)
            in the mouse testis
  JOURNAL   Histochem Cell Biol 147 (1), 27-38 (2017)
   PUBMED   27539077
  REMARK    GeneRIF: The present study identified SPESP1 in the acrosome as the
            primary binding target of Peanut agglutinin (PNA) in the mouse
            testis. Further defining the specific lectin-glycoprotein
            relationships in individual cells will enhance the value of lectin
            histochemistry.
REFERENCE   4  (bases 1 to 3013)
  AUTHORS   Suryavathi,V., Panneerdoss,S., Wolkowicz,M.J., Shetty,J.,
            Sherman,N.E., Flickinger,C.J. and Herr,J.C.
  TITLE     Dynamic Changes in Equatorial Segment Protein 1 (SPESP1)
            Glycosylation During Mouse Spermiogenesis
  JOURNAL   Biol Reprod 92 (5), 129 (2015)
   PUBMED   25761597
  REMARK    GeneRIF: These findings are consistent with the hypothesis that
            SPSPESP1 undergoes significant glycosylation in the testis and that
            the majority of these glycoconjugates are removed by the time sperm
            reach the caput epididymis.
REFERENCE   5  (bases 1 to 3013)
  AUTHORS   Lo,J.C., Jamsai,D., O'Connor,A.E., Borg,C., Clark,B.J.,
            Whisstock,J.C., Field,M.C., Adams,V., Ishikawa,T., Aitken,R.J.,
            Whittle,B., Goodnow,C.C., Ormandy,C.J. and O'Bryan,M.K.
  TITLE     RAB-like 2 has an essential role in male fertility, sperm
            intra-flagellar transport, and tail assembly
  JOURNAL   PLoS Genet 8 (10), e1002969 (2012)
   PUBMED   23055941
REFERENCE   6  (bases 1 to 3013)
  AUTHORS   Fujihara,Y., Murakami,M., Inoue,N., Satouh,Y., Kaseda,K., Ikawa,M.
            and Okabe,M.
  TITLE     Sperm equatorial segment protein 1, SPESP1, is required for fully
            fertile sperm in mouse
  JOURNAL   J Cell Sci 123 (Pt 9), 1531-1536 (2010)
   PUBMED   20375058
  REMARK    GeneRIF: SPESP1 is necessary to produce the fully 'fusion
            competent' sperm.
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AK014843.1.
            
            On Apr 5, 2007 this sequence version replaced NM_025721.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK014843.1, AK015620.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3013              AK014843.1         2-3014
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 33.65 cM"
     gene            1..3013
                     /gene="Spesp1"
                     /gene_synonym="4921508E09Rik"
                     /note="sperm equatorial segment protein 1"
                     /db_xref="GeneID:66712"
                     /db_xref="MGI:MGI:1913962"
     exon            1..178
                     /gene="Spesp1"
                     /gene_synonym="4921508E09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..1317
                     /gene="Spesp1"
                     /gene_synonym="4921508E09Rik"
                     /codon_start=1
                     /product="sperm equatorial segment protein 1 precursor"
                     /protein_id="NP_079997.1"
                     /db_xref="CCDS:CCDS23263.1"
                     /db_xref="GeneID:66712"
                     /db_xref="MGI:MGI:1913962"
                     /translation="MKLVVLVALWLWPSSLLAYPTITVLPDEEQNLNHYVHILQNLIM
                     SVPTKEQDLGKKLSSSRTVDSAEPRSLASKVLLTPGLVSAQDVTPESDVLIRPVDETT
                     NSRTRGFTLRRRRTQSTAFWSIRPNNISVVLRTEEPFIEKEPEPELESSRLPTEPEPE
                     LEPEPEPVAESRQMSEPEEELVTSTTPNKELTGTSRISSMATQPANTQATRITVTVKT
                     TSTMDVSTDSEDVPQLSGQSEIPSAEDLPGRHSLNTRHEDILKKISNINAEIQQGLLG
                     GNNSPEFKEFIKASREHLKRSLALAAAAEHKLEQMYGSNVFPEGRTSDPDNDMEMIIN
                     MLYNSRSKLSDYFNIKRVPSELREKASVVNAELRKILCVDQVEMQSLIKKLLSNNMKI
                     LNILNVP"
     exon            179..3013
                     /gene="Spesp1"
                     /gene_synonym="4921508E09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1476..1481
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spesp1"
                     /gene_synonym="4921508E09Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1495
                     /gene="Spesp1"
                     /gene_synonym="4921508E09Rik"
                     /note="major polyA site"
     regulatory      2988..2993
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spesp1"
                     /gene_synonym="4921508E09Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3013
                     /gene="Spesp1"
                     /gene_synonym="4921508E09Rik"
ORIGIN      
        1 aaggccctga cgtgggacgg taggcctctg acatcccctc ctttcggccc tgaggtaccc
       61 aggcctggtg gccccaggac gttcccgtcg gatggcggag tgctctgaac gacgcccatg
      121 aagctggtgg tcctagttgc gctgtggcta tggccttcgt cactgctggc gtatccaacc
      181 ataactgtgt tgcccgatga agagcagaat ttgaatcatt atgtacacat tttacagaac
      241 ctcataatga gtgtccccac caaggagcag gatctcggga aaaagttgag ttcttcaaga
      301 actgtggata gcgcagagcc aaggtcattg gcatccaagg tgctactcac ccctgggtta
      361 gtctccgctc aagatgtcac ccctgagagc gatgttctga tcagacccgt cgatgaaacg
      421 acaaattccc gtactagagg cttcacgctg aggagaagaa gaacccaaag tacagcattc
      481 tggtccatcc ggccaaacaa tatttctgtt gttttacgca cggaggaacc atttattgaa
      541 aaagaaccgg aaccagaact ggagtctagt cgcctgccta ctgagccgga gccggagctg
      601 gaaccggagc cggagcccgt ggctgaatcc cggcaaatgt cagagcccga agaagagtta
      661 gtgaccagca caacgccaaa caaggagcta acggggacct cacggatatc atccatggcc
      721 acccagccag ctaacaccca ggcaactcgt attactgtaa cagtaaagac caccagcacc
      781 atggatgtct ccacagactc tgaggatgta ccccagctct caggccagtc ggaaatcccg
      841 agtgctgaag acttacctgg acgtcactca ctgaatacga gacatgagga tattttgaaa
      901 aaaatttcca atattaatgc ggagattcag caggggcttc ttggtggcaa taatagtccc
      961 gagttcaagg agttcatcaa ggcctccagg gagcacctga aacggagcct ggccctggcc
     1021 gcagccgccg agcacaagtt ggaacagatg tacggatcta acgtcttccc agagggacgt
     1081 accagcgacc cagacaatga catggaaatg attatcaata tgctgtacaa ctccaggtcc
     1141 aagttgtcag attatttcaa tattaagcgt gtaccatcag agctgagaga aaaggcttcc
     1201 gtcgtgaatg ctgagttgag aaaaatatta tgtgtggatc aggtagaaat gcaaagcctc
     1261 attaagaagc tgttaagcaa taatatgaaa atcctaaata ttcttaatgt cccatgacca
     1321 aactcatgta agcaaaggac ttgttcacag gaaaataagc atagtgattt ttaaagttgc
     1381 ctaaaatatt ttctacaata ttttccatgt gctaacatcc ttgtatgtca tatcataaaa
     1441 tattttcata tgcactaaaa tcctaaaaat tttaaaataa aaatttgctt tgtgagtctg
     1501 tagttttttt tcttctcact aatctaagct gtgaagagct ggccttacag tgttttggat
     1561 ttgcttctac ttagttcaca ggctgcttgg gctgggtcag ggttaaaaga actgcttaca
     1621 gttttgaacc cactttcaga gcatctgcgt tttctgtatg aatggtacac ttgaggctga
     1681 tgtcggaact ctcccatgtg ggtgcatgtt gcttctttgg aaagtgggtg atgtactggg
     1741 gtcattatgg gggactggat ctgtgatgaa ggccatcctc acaatttcct ccctggcctt
     1801 gcatacaaac acattaaaga tttacaatct tttgatagca agtatgactg ataccggtta
     1861 agttagacat tcaatataat aacgaaagtg cagttttgtt tcatgacttg gccagtatta
     1921 tattcaagtt catttactta gcactcaggc tctcactagc actcaagcta gtcatttaca
     1981 gatcaataaa gatttattga ccctctgcta gaacaaaatg ctaggctttg gggatagagt
     2041 gaagagctat gtccctctct ctttgagagt ataacaggga agctagactt tgaacaaaga
     2101 tttccatatt ttaaattttc agttgggacg agctattatg agaagtagct gtctaacttc
     2161 atctagaata tcaagagaag tagccttagg cgagtggcat ttgaggggag gtctgagaga
     2221 cgagatatgc ttgggggaag agaatactgc aggcacagga agaacgtgtg taagtgttga
     2281 gctgaggcca agggtagaag gttcaagaag tgccagagag aatattccaa gacagagcag
     2341 gcacagaagg ccagagagaa gctggcaggc tttgtgctgt gcattaacca tttcaccttt
     2401 atcttgtgct cggctttgta tcagacacca accagtgtgt ttaaatgtaa cactctggct
     2461 acagttttag gggcagactg aggtggacag gagctgtgct ggagccagat ctgccttgga
     2521 ttacatggga ggaagtgaca aaagccagaa caaacagaac cagtgactgg gggagtaacg
     2581 gtgaccagac tgtggtataa agtgatacag aggtttgctt tcacaaaacc actgtacacc
     2641 gtgctttttt taaaaaaata acttactatg aaaaacttca agcattgcaa aagtgagccg
     2701 taacatggta acagtacaga gtcattaccc agtgtgaaca gtcgtgttct aatgggcagt
     2761 agtctctgtc tgtctagccc tatgctaccc cacttcctgt atgctcaact ggatcctcaa
     2821 gaccatttca tctctagctg caccacgtga ggctctctca gcttttgaag aacatttgca
     2881 aatgtttcat aacaatacca tcacgtgcag ttaatggtat ctagtcaatg tttataaagt
     2941 gattagacaa tgggaaattt ggagtttctt tgtatgaatc aagatcaaat aaagttcacg
     3001 cattgctact gcc
//
