LOCUS       NM_019763              12299 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus spen family transcription repressor (Spen), transcript
            variant 1, mRNA.
ACCESSION   NM_019763
VERSION     NM_019763.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 12299)
  AUTHORS   Zhang,J., Yang,Z., Yan,X., Duan,J., Ruan,B., Zhang,X., Wen,T.,
            Zhang,P., Liang,L. and Han,H.
  TITLE     RNA-binding protein SPEN controls hepatocyte maturation via
            regulating Hnf4alpha expression during liver development
  JOURNAL   Biochem Biophys Res Commun 642, 128-136 (2023)
   PUBMED   36577249
  REMARK    GeneRIF: RNA-binding protein SPEN controls hepatocyte maturation
            via regulating Hnf4alpha expression during liver development.
REFERENCE   2  (bases 1 to 12299)
  AUTHORS   Robert-Finestra,T., Tan,B.F., Mira-Bontenbal,H., Timmers,E.,
            Gontan,C., Merzouk,S., Giaimo,B.D., Dossin,F., van IJcken,W.F.J.,
            Martens,J.W.M., Borggrefe,T., Heard,E. and Gribnau,J.
  TITLE     SPEN is required for Xist upregulation during initiation of X
            chromosome inactivation
  JOURNAL   Nat Commun 12 (1), 7000 (2021)
   PUBMED   34853312
  REMARK    GeneRIF: SPEN is required for Xist upregulation during initiation
            of X chromosome inactivation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 12299)
  AUTHORS   Rodermund,L., Coker,H., Oldenkamp,R., Wei,G., Bowness,J.,
            Rajkumar,B., Nesterova,T., Susano Pinto,D.M., Schermelleh,L. and
            Brockdorff,N.
  TITLE     Time-resolved structured illumination microscopy reveals key
            principles of Xist RNA spreading
  JOURNAL   Science 372 (6547) (2021)
   PUBMED   34112668
  REMARK    GeneRIF: Time-resolved structured illumination microscopy reveals
            key principles of Xist RNA spreading.
REFERENCE   4  (bases 1 to 12299)
  AUTHORS   Trotman,J.B., Lee,D.M., Cherney,R.E., Kim,S.O., Inoue,K.,
            Schertzer,M.D., Bischoff,S.R., Cowley,D.O. and Calabrese,J.M.
  TITLE     Elements at the 5' end of Xist harbor SPEN-independent
            transcriptional antiterminator activity
  JOURNAL   Nucleic Acids Res 48 (18), 10500-10517 (2020)
   PUBMED   32986830
  REMARK    GeneRIF: Elements at the 5' end of Xist harbor SPEN-independent
            transcriptional antiterminator activity.
REFERENCE   5  (bases 1 to 12299)
  AUTHORS   Carter,A.C., Xu,J., Nakamoto,M.Y., Wei,Y., Zarnegar,B.J., Shi,Q.,
            Broughton,J.P., Ransom,R.C., Salhotra,A., Nagaraja,S.D., Li,R.,
            Dou,D.R., Yost,K.E., Cho,S.W., Mistry,A., Longaker,M.T.,
            Khavari,P.A., Batey,R.T., Wuttke,D.S. and Chang,H.Y.
  TITLE     Spen links RNA-mediated endogenous retrovirus silencing and X
            chromosome inactivation
  JOURNAL   Elife 9, e54508 (2020)
   PUBMED   32379046
  REMARK    GeneRIF: Spen links RNA-mediated endogenous retrovirus silencing
            and X chromosome inactivation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 12299)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 12299)
  AUTHORS   Oswald,F., Kostezka,U., Astrahantseff,K., Bourteele,S.,
            Dillinger,K., Zechner,U., Ludwig,L., Wilda,M., Hameister,H.,
            Knochel,W., Liptay,S. and Schmid,R.M.
  TITLE     SHARP is a novel component of the Notch/RBP-Jkappa signalling
            pathway
  JOURNAL   EMBO J 21 (20), 5417-5426 (2002)
   PUBMED   12374742
REFERENCE   8  (bases 1 to 12299)
  AUTHORS   Obata,J., Yano,M., Mimura,H., Goto,T., Nakayama,R., Mibu,Y., Oka,C.
            and Kawaichi,M.
  TITLE     p48 subunit of mouse PTF1 binds to RBP-Jkappa/CBF-1, the
            intracellular mediator of Notch signalling, and is expressed in the
            neural tube of early stage embryos
  JOURNAL   Genes Cells 6 (4), 345-360 (2001)
   PUBMED   11318877
REFERENCE   9  (bases 1 to 12299)
  AUTHORS   Newberry,E.P., Latifi,T. and Towler,D.A.
  TITLE     The RRM domain of MINT, a novel Msx2 binding protein, recognizes
            and regulates the rat osteocalcin promoter
  JOURNAL   Biochemistry 38 (33), 10678-10690 (1999)
   PUBMED   10451362
REFERENCE   10 (bases 1 to 12299)
  AUTHORS   Crozet,F., el Amraoui,A., Blanchard,S., Lenoir,M., Ripoll,C.,
            Vago,P., Hamel,C., Fizames,C., Levi-Acobas,F., Depetris,D.,
            Mattei,M.G., Weil,D., Pujol,R. and Petit,C.
  TITLE     Cloning of the genes encoding two murine and human cochlear
            unconventional type I myosins
  JOURNAL   Genomics 40 (2), 332-341 (1997)
   PUBMED   9119401
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY726481.1, AF156529.1, AK137647.1 and AL670285.10.
            
            On Jan 4, 2007 this sequence version replaced NM_019763.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-618               BY726481.1         1-618
            619-1225            AF156529.1         124-730
            1226-1992           AK137647.1         1114-1880
            1993-4924           AF156529.1         1495-4426
            4925-10296          AL670285.10        147594-152965       c
            10297-11331         AF156529.1         9805-10839
            11332-12299         AL670285.10        144114-145081       c
FEATURES             Location/Qualifiers
     source          1..12299
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 74.26 cM"
     gene            1..12299
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /note="spen family transcription repressor"
                     /db_xref="GeneID:56381"
                     /db_xref="MGI:MGI:1891706"
     exon            1..434
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     CDS             352..11283
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /note="isoform 1 is encoded by transcript variant 1; Msx2
                     interacting nuclear target protein; msx2-interacting
                     protein; SMART/HDAC1-associated repressor protein; SPEN
                     homolog, transcriptional regulator"
                     /codon_start=1
                     /product="msx2-interacting protein isoform 1"
                     /protein_id="NP_062737.2"
                     /db_xref="CCDS:CCDS38940.1"
                     /db_xref="GeneID:56381"
                     /db_xref="MGI:MGI:1891706"
                     /translation="MVRETRHLWVGNLPENVREEKIIEHFKRYGRVESVKILPKRGSE
                     GGVAAFVDFVDIKSAQKAHNSVNKMGDRDLRTDYNEPGTIPSAARGLDETVSIASRSR
                     EVSGFRGSAGGPAYGPPPSLHAREGRYERRLDGASDNRERAYEHSAYGHHERGTGAFD
                     RTRHYDQDYYRDPRERTLQHGLYYTSRSRSPNRFDAHDPRYEPRAREQFTLPSVVHRD
                     IYRDDITREVRGRRPERSYQHSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRS
                     RSSSSDSISSSSSSSSNTDSSDSSSTASDDSPARSVQSAAVPAPTSQLLSSLEKDEPR
                     KSFGIKVQNLPVRSTDTSLKDGLFHEFKKFGKVTSVQIHGASEERYGLVFFRQQEDQE
                     KALTASKGKLFFGMQIEVTAWVGPETESENEFRPLDERIDEFHPKATRTLFIGNLEKT
                     TTYHDLRNIFQRFGEIVDIDIKKVNGVPQYAFLQYCDIASVCKAIKKMDGEYLGNNRL
                     KLGFGKSMPTNCVWLDGLSSNVSDQYLTRHFCRYGPVVKVVFDRLKGMALVLYSEIED
                     AQAAVKETKGRKIGGNKIKVDFANRESQLAFYHCMEKSGQDMRDFYEMLTERRAGQMA
                     QSKHEDWSADAQSPHKCREERRGSYEYSQERTYYENVRTPGTYPEDSRRDYPARGREF
                     YSEWETYQGEYYDSRYYDEPREYREYRSDPYEQDIREYSYRQRERERERERFESDRDH
                     ERRPIERSQSPVHLRRPQSPGVSPAHSERLPSDSERRLYRRSSERSGSCSSVSPPRYD
                     KLEKARLERYTKNEKADKERTFDPERVERERRIVRKEKGEKDKAERQKRKGKAHSPSS
                     QPSETEQENDREQSPEKPRGSTKLSRDRADKEGPAKNRLELVPCVVLTRVKEKEGKVI
                     EHPPPEKLKARLGRDTTKASALDQKPQAAQGEPAKSDPARGKALREKVLPSHAEVGEK
                     EGRTKLRKHLKAEQTPELSALDLEKLEARKRRFADSGLKIEKQKPEIKKTSPETEDTR
                     ILLKKQPDTSRDGVLLREGESERKPVRKEILKRESKKTKLERLNSALSPKDCQDPAAV
                     SAGSGSRPSSDVHAGLGELTHGSVETQETQPKKAIPSKPQPKQLQLLENQGPEKEEVR
                     KNYCRPREEPAEHRAGQEKPHGGNAEEKLGIDIDHTQSYRKQMEQSRRKQRMEMEIAK
                     AEKFGSPKKDVDDYERRSLVHEVGKPPQDVTDDSPPSKKRRTDHVDFDICTKRERNYR
                     SSRQISEDSERTSCSPSVRHGSFHDDDDPRGSPRLVSVKGSPKGDEKGLPYPNAAVRD
                     DPLKCNPYDSGKREQTADTAKIKLSVLNSEGEPSRWDPPMKQDPSRFDVSFPNSVIKR
                     DSLRKRSVRDLEPGEVPSDSDEDAEHRSQSPRASSFYDSPRLSFLLRDRDQKLRERDE
                     RLASSLERNKFYSFALDKTITPDTKALLERAKSLSSSREENWSFLDWDSRFANFRNNK
                     DKEKVDSAPRPIPSWYMKKKKIRTDSEGKLADKKDERREEEQERQELFASRFLHSSIF
                     EQDSKRLQHLERKSEEPDLPPGGLYGRQASEGANSTSDSVQEPVVLFHSRFMELTRMQ
                     QKEKEKDQKPKEAEKQEEPETHPKTPEPAAETKEPEPKAPVSAGLPAVTVTVVTPEPA
                     SSAPEKAEEAAEAPSPAGEKPAEPAPVSEETKLVSEPASVPVEQPRQSDVPPGEDSRD
                     SQDSAALAPSAPQESAATDAVPCVNAEPLTPGTTVSQVESSVDPKPSSPQPLSKLTQR
                     SEEAEEGKVEKPDTTPSTEPDATQNAGVASEAQPPASEDVEANPPVAAKDRKTNKSKR
                     SKTSVQAAAASVVEKPVTRKSERIDREKLKRSSSPRGEAQKLLELKMEAEKITRTASK
                     SSGGDTEHPEPSLPLSRSRRRNVRSVYATMTDHESRSPAKEPVEQPRVTRKRLERELQ
                     EAVVPPTTPRRGRPPKTRRRAEEDGEHERKEPAETPRPAEGWRSPRSQKSAAAAGPQG
                     KRGRNEQKVEAAAEAGAQASTREGNPKSRGEREAASEPKRDRRDPSTDKNGPDTFPVE
                     VLERKPPEKTYKSKRGRARSTRSAMDRAAHQRSLEMAARAAGQAADKEAGPAAASPQE
                     SESPQKGSGSSPQLANNPADPDREAEEESASASTAPPEGTQLARQIELEQAVQNIAKL
                     PEPSAAAASKGTATATAASEEPAPEHGHKPAHQASETELAAAIGSIISDASGEPENFS
                     APPSVPPGSQTHPREGMEPGLHEAESGILETGTATESSAPQVSALDPPEGSADTKETR
                     GNSGDSVQEAKGSKVEVTPPRKDKGRQKTTRRRKRNANKKVVAITETRASEAEQTQSE
                     SPAAEEATAATPEAPQEEKQSEKPPSPPAECTFDPSKTPPAESLSQENSAAEKTPCKA
                     PVLPALPPLSQPALMDDGPQARFKVHSIIESDPVTPPSDSGIPPPTIPLVTIAKLPPP
                     VIPGGVPHQSPPPKVTEWITRQEEPRAQSTPSPALPPDTKASDMDTSSSTLRKILMDP
                     KYVSATGVTSTSVTSAIAEPVSAPCLQEAPAPPCDPKHPPLEGVSAAAVPNADTQASE
                     VPVAADKEKVAPVIAPKITSVISRMPVSIDLENSQKITLAKPAPQTLTGLVSALTGLV
                     NVSLVPVNALKGPVKGSVATLKGLVSTPAGPVNLLKGPVNVLTGPVNVLTTPVSATVG
                     TVNAAPGPVTAACGVTATTGTAAVTGAVTAPAAKGKQRASSNENSRFHPGSMSVIDDR
                     PADTGSGAGLRVNTSEGVVLLSYSGQKTEGPQRISAKISQIPPASAMDIEFQQSVSKS
                     QVKADSITPTQSAPKGPQTPSAFANVAAHSTLVLTAQTYNASPVISSVKTDRPSLEKP
                     EPIHLSVSTPVTQGGTVKVLTQGINTPPVLVHNQLVLTPSIVTTNKKLADPVTLKIET
                     KVLQPANLGPTLTPHHPPALPSKLPAEVNHVPSGPSTPADRTIAHLATPKPDTHSPRP
                     TGPTPGLFPRPCHPSSTTSTALSTNATVMLAAGIPVPQFISSIHPEQSVIMPPHSITQ
                     TVSLGHLSQGEVRMSTPTLPSITYSIRPETLHSPRAPLQPQQIEARAPQRVGTPQPAT
                     TGVPALATQHPPEEEVHYHLPVARAAAPVQSEVLVMQSEYRLHPYTVPRDVRIMVHPH
                     VTAVSEQPRATEGVVKVPPANKAPQQLVKEAVKTSDAKAVPAPAPVPVPVPVPTPAPP
                     PHGEARILTVTPSSQLQGLPLTPPVVVTHGVQIVHSSGELFQEYRYGDVRTYHAPAQQ
                     LTHTQFPVASSISLASRTKTSAQVPPEGEPLQSTQSAQPAPSTQATQPIPPAPPCQPS
                     QLSQPAQPPSGKIPQVSQEAKGTQTGGVEQTRLPAIPTNRPSEPHAQLQRAPVETAQP
                     AHPSPVSVSMKPDLPSPLSSQAAPKQPLFVPANSGPSTPPGLALPHAEVQPAPKQESS
                     PHGTPQRPVDMVQLLKKYPIVWQGLLALKNDTAAVQLHFVSGNNVLAHRSLPLSEGGP
                     PLRIAQRMRLEASQLEGVARRMTVETDYCLLLALPCGRDQEDVVSQTESLKAAFITYL
                     QAKQAAGIINVPNPGSNQPAYVLQIFPPCEFSESHLSRLAPDLLASISNISPHLMIVI
                     ASV"
     exon            435..755
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            756..1238
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1399
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1600
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1752
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1878
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..1992
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2106
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2207
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            2208..2276
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            2277..10296
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            10297..10797
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            10798..10992
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            10993..11151
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
     exon            11152..12299
                     /gene="Spen"
                     /gene_synonym="Mint; mKIAA0929"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtccggcagc cacagcgccg gctccgtcgt agtcgcggcc gcccgggtct cgctcgcccc
       61 tcctcccgct cccccgcccg ccgccgcccg cgcgcatgcg ccgccggagc gcgagggtcg
      121 gcttcgggtg tttggtggcg gcagagcgga gctgcgaggc ccgagagtca gaacctgggg
      181 gagagggatg gtctctgcac cggggggagc cggaggagcc gccgccgctg ccgacgccac
      241 cgccgcagcc gccgccgccg ccgccccggc gcccgcctcc ctgcgctgac ggccgcgtac
      301 gaagccggcg agggggagcc agccaccgcg gtcgccggca caccgcccag catggtccgg
      361 gaaaccaggc acctctgggt gggaaactta ccggagaacg tgcgggaaga gaagatcatc
      421 gagcatttca aacgatatgg ccgcgtggaa agtgtcaaga ttctccccaa gcgggggtct
      481 gaaggaggag tggctgcctt tgtggatttt gtggacatca aaagtgcaca gaaagctcac
      541 aactccgtca acaaaatggg agaccgagac ctccgcacgg attataatga gccaggaact
      601 attccgagtg ctgcccgggg actggacgag acagtttcca tagcgtctcg tagtagagag
      661 gtttctgggt tcagaggaag tgctggaggg cctgcttatg gtcccccacc atcacttcat
      721 gcacgagaag gacgctatga gcggagactc gatggggctt cagataacag ggagcgtgct
      781 tatgaacata gtgcctatgg acaccatgaa cgggggacag gagcatttga tcggacaaga
      841 cattacgatc aggattacta tagagaccct cgagagcgga ctttacaaca cgggctctat
      901 tacacttctc ggagtcgaag tccaaaccgc tttgatgctc atgacccccg atacgaacct
      961 agggcccggg agcagtttac actgcccagt gtggtacaca gggatatcta cagggacgac
     1021 attacccggg aggtacgagg cagaaggcca gagcgcagtt accaacacag caggagtcgc
     1081 tcaccacatt cgtcccagtc tagaaaccag tccccgcaga ggctggctag ccaagcgtcc
     1141 agacccacca ggtcccccag cggcagcggc tccaggagtc gatcctccag tagtgactcc
     1201 atcagcagca gcagcagcag cagcagcaac actgacagta gcgactccag cagcacggcc
     1261 agtgacgact ctccggcccg ctctgttcag tcggctgcag tccccgcacc cacttcccag
     1321 ttgctttcgt cccttgaaaa agatgagccc cggaaaagtt ttgggatcaa agttcagaat
     1381 cttccagtac gctctacaga cacaagcctt aaagacggcc ttttccatga gtttaagaag
     1441 tttggcaagg tgacgtcagt gcagatccac ggggcctccg aggagagata cggcctggtg
     1501 ttcttccggc agcaagagga ccaagaaaaa gcattgaccg catccaaggg gaagctcttc
     1561 ttcggcatgc agatcgaggt gactgcatgg gtaggaccag aaacagagag tgaaaatgag
     1621 tttcggccct tggatgagag gatagatgaa ttccatccca aagcaacgag aactcttttt
     1681 attgggaacc ttgaaaaaac gactacttac cacgaccttc gaaacatctt tcagcgcttt
     1741 ggagaaattg tggacattga cattaagaag gtgaacgggg tgcctcagta cgcgttcctg
     1801 cagtactgtg atatcgctag tgtgtgtaag gccatcaaga agatggacgg agagtacctt
     1861 ggaaataacc gcctcaagct gggttttgga aagagcatgc ctaccaactg tgtgtggtta
     1921 gatgggcttt cttcaaatgt gtccgatcag tatttaactc ggcatttctg ccgatacgga
     1981 cctgtggtaa aggtggtgtt tgaccgcttg aaaggcatgg ctctggttct ctacagcgag
     2041 atcgaggatg cacaggcggc tgtcaaggag accaagggca ggaaaattgg tgggaataag
     2101 attaaggtgg actttgcaaa tcgggaaagc caactggcat tttatcactg catggagaaa
     2161 tctggtcagg atatgagaga cttctatgaa atgctaacag agagaagggc agggcagatg
     2221 gctcagtcca agcatgaaga ctggagtgca gatgcccaga gcccacataa atgccgagag
     2281 gaacgaagag ggtcctatga gtacagtcaa gaacggacat actatgaaaa tgttcgcact
     2341 ccaggcacct accctgagga ctccaggagg gactatccgg ctcgggggag agagttttac
     2401 tcggagtggg agacgtacca aggagagtat tatgactccc ggtactatga tgagcctcgc
     2461 gaatacagag aatataggag tgatccctat gagcaggata ttcgggagta cagctatagg
     2521 cagcgagaac gagagcgaga acgggagaga ttcgaatctg accgggacca cgagaggaga
     2581 cccatagagc gaagccagag tcccgtgcac ttgcgtcgtc cacagagccc tggggtgtct
     2641 cctgcgcatt cggagagatt gccaagtgac tctgagagga ggctgtaccg ccggtcctcg
     2701 gagcgcagcg gcagctgcag ctcagtgtcc cccccacggt acgataagct ggagaaggct
     2761 cggctggagc gctacacaaa aaacgaaaag gcggacaaag agcgcacttt cgatcccgag
     2821 cgggtcgaga gggagagacg aatagttcgc aaggagaagg gagaaaagga caaagctgag
     2881 aggcagaagc ggaaaggaaa ggctcactcc cctagttctc agccttcgga aaccgagcag
     2941 gaaaatgacc gagagcagag ccccgagaaa ccgcggggct ccaccaaact cagcagagac
     3001 agggccgaca aggaagggcc agccaagaac cgcctggagc tcgtgccttg cgtggttctg
     3061 acccgagtaa aggaaaagga gggcaaggtc atcgagcacc cacccccaga aaagctcaaa
     3121 gccaggctcg gccgtgacac taccaaggca tcggccctgg accagaaacc ccaggccgct
     3181 cagggggagc ctgccaagtc ggacccggcc cgagggaaag cgctgaggga gaaggtcctc
     3241 ccgagccacg cggaggtggg ggagaaggag ggcaggacta agctccggaa acacctcaag
     3301 gccgagcaga cgcccgagct cagcgctctg gacctggaga agctggaagc caggaaaagg
     3361 cgctttgcag attctggctt gaaaatagaa aagcaaaaac cagaaatcaa gaaaaccagc
     3421 cccgagacag aggatactcg aatactttta aagaagcagc ctgacacatc aagagatggt
     3481 gttttgctga gggaaggaga gtctgaaaga aagcctgtga gaaaagaaat ccttaaaagg
     3541 gaatcaaaaa aaactaagct ggaaagactt aattcagctc tcagccccaa agactgccag
     3601 gatcctgctg ctgtctctgc agggtctggc tctcggccca gctcagacgt acacgcagga
     3661 ctgggagaac taacacacgg atctgtggag actcaagaaa cccagcccaa aaaagccatc
     3721 ccctcaaaac ctcagcccaa acagctgcag ctcctagaga atcaagggcc tgagaaagag
     3781 gaagtgagga aaaactattg ccggcctcgt gaggaaccag cggaacacag agcaggccag
     3841 gagaagcccc acggagggaa tgcggaagag aaacttggca ttgacattga tcacacgcag
     3901 agctaccgga aacagatgga gcagagccgc cggaagcagc ggatggagat ggagatcgcc
     3961 aaggctgaga agtttggcag tccgaagaaa gatgtagatg actacgaaag gcgcagcctt
     4021 gtccacgagg tgggcaagcc ccctcaggat gtcactgatg actccccgcc cagcaagaag
     4081 cgaaggacgg atcacgtgga tttcgatatc tgtaccaaaa gagagagaaa ctaccgaagt
     4141 tcacgccaga tcagcgagga ctcagaaaga accagctgct ctccgagtgt ccgccacggc
     4201 tccttccatg acgatgatga ccccaggggc tcccctaggc tagtgtcagt aaaagggtcc
     4261 cctaaagggg atgagaaagg cctcccctat cctaacgcag cagtgagaga cgatccatta
     4321 aaatgcaatc cttacgattc tggcaagaga gaacagacag cagacacggc caaaatcaaa
     4381 ctgtctgtct tgaattctga aggggaacca agccggtggg accctccgat gaaacaggac
     4441 cccagccggt ttgatgtcag tttcccaaac agtgtaatta agagagacag tctacgaaag
     4501 aggtctgtgc gcgatctgga acctggtgag gtgccctctg attctgatga ggacgccgag
     4561 cacaggtccc agtcccccag agcttccagc ttctatgaca gccctcggct gtctttttta
     4621 ttgagggaca gagaccaaaa actacgtgag cgagatgaaa gactcgctag ctctttagaa
     4681 aggaataaat tttactcttt tgcactggat aagacaatca caccagacac taaagctttg
     4741 cttgaaagag ccaaatccct ctcatcatcc cgtgaagaaa attggtcttt ccttgactgg
     4801 gactcgcggt ttgctaattt tcgaaacaac aaagataaag aaaaggttga ctcggccccc
     4861 aggcctattc cgtcctggta catgaagaag aagaaaatca ggacggactc ggaggggaaa
     4921 ctggctgata aaaaggacga gcgcagggag gaggagcagg agaggcagga gttgttcgcc
     4981 tctcggttct tacacagctc catctttgag caagactcca agaggctgca gcaccttgag
     5041 aggaagagcg aggagcccga cctccccccc ggggggctgt acggcaggca ggcgtctgag
     5101 ggtgcaaaca gcacgagtga ttcggtgcag gagccagtgg tcctgttcca cagcagattc
     5161 atggagctga cgcggatgca gcagaaggag aaggagaagg accagaagcc caaagaggct
     5221 gagaagcagg aagagccgga gactcatccc aagactccag agcctgctgc agagactaaa
     5281 gagccggagc cgaaagcccc tgtttcagct gggcttcctg ccgtcaccgt caccgtcgtg
     5341 acaccagagc cagcatcctc cgcccctgag aaggctgagg aggcagcgga ggctccttca
     5401 ccagcaggag agaagcctgc agagcccgct cctgtctcgg aagaaacaaa gcttgtgtct
     5461 gagcctgcgt ctgtacccgt ggagcagccc aggcagtcag atgtaccccc cggagaggac
     5521 agccgggata gccaggacag cgctgccttg gcaccttcag ctcctcaaga aagcgcagct
     5581 actgacgccg tcccttgtgt gaatgctgaa cctctgactc ccggaaccac agtttcccag
     5641 gtagaaagca gtgtagaccc caaacctagc agtccccagc ccctttcaaa actgactcag
     5701 aggtccgagg aagccgaaga ggggaaagtg gagaagccag acacaactcc aagcactgag
     5761 cctgacgcca ctcagaacgc tggggttgca tcagaagctc agccccccgc ttctgaagat
     5821 gtagaagcca atcctccagt cgctgcaaaa gacaggaaga ctaacaagag caagcgttcc
     5881 aagacgtccg tccaggcggc tgccgccagc gtcgtggaga agcctgtcac caggaagagc
     5941 gagaggatag accgggaaaa gctgaagagg tccagctctc ctcgtggaga agcacagaag
     6001 ctgctggaac taaagatgga ggcagagaag attacaagga ctgcgtcgaa aagctctggg
     6061 ggagacacgg agcaccctga gcctagcttg cctctcagca ggagcaggcg ccggaacgtg
     6121 aggagtgttt atgccaccat gaccgaccac gagagccggt ccccggccaa ggagcctgtg
     6181 gagcagccca gagtgaccag gaagagactg gagcgggagc tgcaggaggc ggtggtacct
     6241 cccaccaccc ctcggagggg aaggcccccc aagacccgcc ggcgtgccga ggaagacggt
     6301 gagcatgaga ggaaagagcc tgcagagaca cccaggcccg ctgagggctg gcggtcaccg
     6361 aggtctcaga agtcggcagc tgctgctgga ccccaaggga aaagggggag gaatgagcag
     6421 aaggttgagg ctgccgctga ggccggtgcc caggcgagca cgagggaagg taaccctaag
     6481 tccagaggcg agagagaggc agcaagtgag cccaaacggg acaggagaga ccccagcaca
     6541 gacaagaacg gacccgacac cttcccagtt gaggtcctgg agagaaaacc tcctgagaaa
     6601 acctacaaat caaagagggg ccgtgctcgg agcacgaggt cagcgatgga cagggccgcg
     6661 catcagagaa gcctggaaat ggccgcccgg gcagcagggc aggcagcaga caaggaagct
     6721 gggcctgcgg cggcctctcc tcaggagagt gagagccccc agaagggaag tggctcctca
     6781 cctcagctgg caaacaaccc agccgatccc gacagggagg cagaggagga aagtgcgtct
     6841 gcctccacgg cgcccccaga aggcacgcag ctagccaggc agatagagct ggagcaggcc
     6901 gtgcagaaca tcgcaaagct ccccgagccc tctgctgctg ccgctagcaa gggcacggcc
     6961 acggccacgg cagcatctga agagcctgcc ccagagcatg gccacaagcc agcacaccag
     7021 gccagcgaaa ctgaactggc tgcggccatt ggctccatca tcagtgacgc ctctggagag
     7081 ccagagaact tctccgctcc tccctccgtt cccccaggat ctcagacaca tccacgggaa
     7141 ggaatggagc cagggctcca tgaggccgag tcgggaatct tggagacagg gacagcaaca
     7201 gagtcttctg ccccacaagt cagtgccttg gatccaccgg aaggttctgc agacaccaag
     7261 gaaaccagag gaaacagtgg tgactctgta caggaagcca aggggtctaa agtggaagtc
     7321 actcctcccc ggaaagacaa agggcgccaa aagacaacac gacgccggaa aaggaatgcc
     7381 aacaagaaag tggtcgccat cacagagact cgtgcctctg aggctgagca gactcagagt
     7441 gagagccccg ctgcagagga ggcgacagca gcaaccccgg aagctcccca ggaagagaaa
     7501 cagagtgaga agcctccctc ccctcctgcg gagtgcacct tcgacccaag caagaccccg
     7561 cctgctgaga gtctgtccca ggaaaacagt gctgcagaaa agacaccgtg caaagcccct
     7621 gtgctccctg ccctgccccc cctttcccag ccagcactga tggatgatgg gccccaagcc
     7681 aggttcaagg tgcactcgat catcgagagt gaccctgtga ccccacccag cgactcaggc
     7741 atacctcctc ccacaatccc cttagtgacc atagcaaagc tcccacctcc agtcattcct
     7801 gggggagtcc cacatcagag tcccccccct aaggtgacag agtggatcac aagacaggaa
     7861 gagcctcggg ctcagtccac cccatctcca gcccttcccc cagacacaaa ggcctctgat
     7921 atggacacca gctccagtac actgaggaag atcctcatgg accccaaata tgtgtctgcc
     7981 acaggagtta cgtccacaag tgtcacttca gccattgcag agcccgtgag tgcgccttgt
     8041 ctgcaggagg cgccggcccc tccctgtgac cctaagcatc ctcccttaga aggcgtgtca
     8101 gcagccgcag taccaaatgc tgacacacaa gcctcagagg ttccggtggc tgccgacaaa
     8161 gagaaggtgg ctccggtcat tgcccctaaa atcacgtcgg ttattagccg gatgcccgtt
     8221 agcattgacc tggagaactc gcagaagatc acgttggcaa agccagctcc tcagaccctg
     8281 actggcctgg tgagtgccct gactggtctg gtgaatgtct ctctggtccc ggtgaatgcc
     8341 ttaaagggcc ctgtgaaggg ctcagtagcc accctgaaag gtttggtgag cacccctgct
     8401 gggcccgtga atctcctaaa ggggcccgtg aatgtcctca cggggcctgt caatgtcctg
     8461 actactcctg tgagtgccac agtgggcacg gtgaacgctg cccccggccc cgtgactgct
     8521 gcctgtggcg tgacggccac aacaggcaca gcagccgtga cgggtgcagt gactgcccca
     8581 gcagccaagg gcaagcagcg ggccagtagc aatgagaaca gccgcttcca ccctgggtcc
     8641 atgtcagtga ttgatgaccg accagcagac acgggctctg gcgcggggct gcgggtgaat
     8701 acatcagaag gggtcgtgct gctgagctac tcgggacaga agacggaagg cccccagcgg
     8761 atcagtgcta agatcagcca gatccctcca gccagcgcaa tggacatcga gtttcagcag
     8821 tcagtatcca agtcccaagt gaaagcagac tccatcaccc cgacccagtc tgcacccaaa
     8881 ggccctcaga ccccttcagc ctttgccaac gtggccgccc attctaccct ggtactgact
     8941 gcccagacat acaatgcctc tcccgtgatc tcctccgtga agacggaccg gccatccttg
     9001 gagaagcccg agcccattca cctctcggtg tccacccctg tgacccaggg tggcacagtg
     9061 aaggttctca cccagggcat caacaccccc ccagtgctcg tccacaacca acttgtcctc
     9121 accccgagca tcgtcaccac caacaagaag cttgctgacc ctgtcactct gaaaatcgag
     9181 accaaggtcc ttcagccagc taacctgggg cccaccctca ctccccacca cccccctgcc
     9241 ctgcccagca aactgcctgc agaggtgaac catgttcctt ctggacccag taccccagca
     9301 gatcggacca tcgcccactt ggcaaccccc aaaccagata ctcattcacc tcgccctact
     9361 gggcctacac caggcctgtt ccctaggcca tgccacccca gcagcacgac ctccacagcg
     9421 ctgtccacca acgccaccgt catgctggct gccggcatcc cggtgccgca gttcatctcc
     9481 agcatacacc cagagcagtc tgtcatcatg cccccccaca gcatcactca gacagtgtct
     9541 ctaggtcacc tgtcccaggg ggaggtgaga atgagcaccc ccaccctgcc cagcatcacc
     9601 tacagcatcc ggccagagac gctgcactct cctcgagccc ctctgcagcc ccagcagata
     9661 gaggcgaggg ccccacagcg tgtgggcacg cctcagccag ccacgactgg tgtgcctgct
     9721 ctggccacac agcaccctcc tgaggaagag gttcattatc acctccctgt ggctcgagct
     9781 gcagccccgg tgcagtcaga agtgctggtc atgcagtccg agtaccggtt gcacccgtac
     9841 acggttcccc gggacgtgcg gatcatggtg catccacatg tgactgctgt cagtgaacag
     9901 cccagggcca cagagggggt ggtaaaggtg ccaccagcca acaaggcccc ccagcaactg
     9961 gtgaaggaag cggttaagac ttcagatgcc aaagcagtcc cggccccagc ccctgtcccc
    10021 gtccctgtcc ctgtccctac ccctgccccg ccccctcatg gtgaagcccg cattctcacg
    10081 gtcaccccca gcagccagct tcaggggctg cctctgaccc cacctgtggt cgtaacccac
    10141 ggggtgcaga ttgtgcactc cagtggtgag ctgttccaag aatacaggta cggtgacgtc
    10201 cgcacctacc acgcccccgc tcagcagctc acacacactc agtttcctgt cgcctcctcc
    10261 atcagcctag cctccaggac caagacttct gcccaggtgc cccctgaagg tgagcccttg
    10321 cagtccacgc agtctgcaca gcctgccccc tctacgcagg ccacgcagcc tataccacct
    10381 gccccgccct gccagccctc ccaactcagc cagcctgccc agccaccgag tggcaagatt
    10441 cctcaggtct cccaagaagc aaaggggacg cagacaggag gggtggagca gactcgcctc
    10501 ccagccatcc ccacaaacag gccatctgag ccacacgctc agcttcagag ggcgccggtg
    10561 gaaacagccc agccagccca cccctcccct gtgtccgtct ccatgaagcc agacctccca
    10621 agccctcttt cctctcaggc tgccccaaag cagccattgt ttgtccctgc aaactcaggc
    10681 ccaagcaccc cacctggact ggctctgcct cacgctgaag tccagccagc tcccaaacaa
    10741 gagtcttctc cacacgggac accacaacgg cctgtggata tggtccagct gctgaagaag
    10801 taccccatcg tgtggcaggg cctgctggcc ctcaagaacg acacggctgc tgtgcagctc
    10861 cacttcgtct ctggcaacaa tgtcctggct catcggtccc tgcctctctc tgaaggtggt
    10921 cccccactgc ggatcgccca gaggatgcga ctagaggcct cacagctgga aggggtcgcc
    10981 aggagaatga cagtggaaac cgactactgc ctgctgctgg ctctgccctg tggccgtgac
    11041 caggaggatg tggtgagcca gaccgagtcc ctcaaggctg ccttcatcac ttacctgcag
    11101 gccaagcaag cagcagggat tatcaatgtc cccaaccctg gctccaatca gcctgcctac
    11161 gtgcttcaga tcttcccacc ctgtgagttc tccgagagtc acctctcccg gctggcccct
    11221 gacctccttg ccagcatctc caacatctct cctcacctca tgattgtcat tgcctcggtg
    11281 tgaggctctg aacggtcgcc acctcagggc attttcctga ggctctgctg caaaaaaaaa
    11341 aaaaaaaatg aacaccgtgg acaacccagc caggcagaga ggcaggctgc caggcgccac
    11401 cagctgcccc gcccttcggc tgcccagctc cgtggataga cctcctctgg gggtggtgct
    11461 gcttgtatgt ttacatgaaa ttctcccagg acagaccacc aaccatggac acagagacct
    11521 taggggctgg gcttctctcc gcacatggag aacaggagcc gggccctggg atccttctgt
    11581 tctgttaaac agaacagaac agcctttgca ctaaattagt gactcgcgca gtgaacacag
    11641 gctgcgatgc tctggacatg tgtgcatgag cacgccgtgg actctcgtgc aggacttggg
    11701 actcgtgctg aagccttggg gtcaaggaac atttcagtgg gtttttgccc catctcctcc
    11761 tcccgtcccc tgctcatggg acatgttatc tttcttaatt ctcctgccgc cgccgccacc
    11821 accaccacca ccaccaccac caccaccttc agctcatcgg atgtacagtt tacagttgag
    11881 taacagtgaa cggaaggatt ttctttcttg gtcggatgtg cagaacttgg gatgtgtata
    11941 tataaatata taatatatat aaatatatat aatactgact taaatcaaat ttccctgaca
    12001 tacatttttt ttaatctgtg ccaaaaatgt gttttcaaag aaaatcttat tttcatactc
    12061 aagactttgt accgtcactc atttgtataa gtgcgcttcg tcactgcacg ggccctgctc
    12121 ctgcaacatg gaactgtcgc gtagcacctg tacaagagac ccggccactc cactccctcc
    12181 tgtgacctgg acctctccct gacttcctaa cacttattaa tttatgaaac tgtttttctc
    12241 agcgcagttt tgttctgtgt gtccattgga ttacaaactt tattaaaaaa tacaaaaca
//
