LOCUS       NM_001305042            5565 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus sperm flagellar 2 (Spef2), transcript variant 1, mRNA.
ACCESSION   NM_001305042 XM_006520087
VERSION     NM_001305042.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Qu,R., Zhang,Z., Wu,L., Li,Q., Mu,J., Zhao,L., Yan,Z., Wang,W.,
            Zeng,Y., Liu,R., Dong,J., Li,Q., Sun,X., Wang,L., Sang,Q., Chen,B.
            and Kuang,Y.
  TITLE     ADGB variants cause asthenozoospermia and male infertility
  JOURNAL   Hum Genet 142 (6), 735-748 (2023)
   PUBMED   36995441
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Miyata,H., Morohoshi,A. and Ikawa,M.
  TITLE     Analysis of the sperm flagellar axoneme using gene-modified mice
  JOURNAL   Exp Anim 69 (4), 374-381 (2020)
   PUBMED   32554934
  REMARK    Review article
REFERENCE   3  (bases 1 to 5565)
  AUTHORS   McKenzie,C.W. and Lee,L.
  TITLE     Genetic interaction between central pair apparatus genes CFAP221,
            CFAP54, and SPEF2 in mouse models of primary ciliary dyskinesia
  JOURNAL   Sci Rep 10 (1), 12337 (2020)
   PUBMED   32704025
  REMARK    GeneRIF: Genetic interaction between central pair apparatus genes
            CFAP221, CFAP54, and SPEF2 in mouse models of primary ciliary
            dyskinesia.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5565)
  AUTHORS   Tu,C., Nie,H., Meng,L., Wang,W., Li,H., Yuan,S., Cheng,D., He,W.,
            Liu,G., Du,J., Gong,F., Lu,G., Lin,G., Zhang,Q. and Tan,Y.Q.
  TITLE     Novel mutations in SPEF2 causing different defects between flagella
            and cilia bridge: the phenotypic link between MMAF and PCD
  JOURNAL   Hum Genet 139 (2), 257-271 (2020)
   PUBMED   31942643
  REMARK    GeneRIF: Mutations in SPEF2 are associated with multiple
            morphological abnormalities of the sperm flagella and primary
            ciliary dyskinesia.
REFERENCE   5  (bases 1 to 5565)
  AUTHORS   Lehti,M.S., Henriksson,H., Rummukainen,P., Wang,F.,
            Uusitalo-Kylmala,L., Kiviranta,R., Heino,T.J., Kotaja,N. and
            Sironen,A.
  TITLE     Cilia-related protein SPEF2 regulates osteoblast differentiation
  JOURNAL   Sci Rep 8 (1), 859 (2018)
   PUBMED   29339787
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5565)
  AUTHORS   Lehti,M.S., Zhang,F.P., Kotaja,N. and Sironen,A.
  TITLE     SPEF2 functions in microtubule-mediated transport in elongating
            spermatids to ensure proper male germ cell differentiation
  JOURNAL   Development 144 (14), 2683-2693 (2017)
   PUBMED   28619825
  REMARK    GeneRIF: Investigated the function of SPEF2 during spermatogenesis
            using a male germ cell-specific Spef2 knockout model. In addition
            to defects in sperm tail development, we observed a duplication of
            the basal body and failure in manchette migration resulting in an
            abnormal head shape.
REFERENCE   7  (bases 1 to 5565)
  AUTHORS   Finn,R., Evans,C.C. and Lee,L.
  TITLE     Strain-dependent brain defects in mouse models of primary ciliary
            dyskinesia with mutations in Pcdp1 and Spef2
  JOURNAL   Neuroscience 277, 552-567 (2014)
   PUBMED   25073043
REFERENCE   8  (bases 1 to 5565)
  AUTHORS   McKenzie,C.W., Klonoski,J.M., Maier,T., Trujillo,G., Vitiello,P.F.,
            Huber,V.C. and Lee,L.
  TITLE     Enhanced response to pulmonary Streptococcus pneumoniae infection
            is associated with primary ciliary dyskinesia in mice lacking Pcdp1
            and Spef2
  JOURNAL   Cilia 2 (1), 18 (2013)
   PUBMED   24360193
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 5565)
  AUTHORS   Sironen,A., Kotaja,N., Mulhern,H., Wyatt,T.A., Sisson,J.H.,
            Pavlik,J.A., Miiluniemi,M., Fleming,M.D. and Lee,L.
  TITLE     Loss of SPEF2 function in mice results in spermatogenesis defects
            and primary ciliary dyskinesia
  JOURNAL   Biol Reprod 85 (4), 690-701 (2011)
   PUBMED   21715716
  REMARK    GeneRIF: Data indicate that SPEF2 is required for cilia
            function/motility; defect in SPEF2 results in primary ciliary
            dyskinesia. SPEF2 plays important role in spermatogenesis and sperm
            flagellum assembly. (base/AA sequence data submitted to GenBank)
REFERENCE   10 (bases 1 to 5565)
  AUTHORS   Sironen,A., Hansen,J., Thomsen,B., Andersson,M., Vilkki,J.,
            Toppari,J. and Kotaja,N.
  TITLE     Expression of SPEF2 during mouse spermatogenesis and identification
            of IFT20 as an interacting protein
  JOURNAL   Biol Reprod 82 (3), 580-590 (2010)
   PUBMED   19889948
  REMARK    GeneRIF: The crucial importance of SPEF2 in sperm differentiation
            and involvement of SPEF2 in structuring of the sperm tail.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK048961.1, HQ856050.1, AC133959.4 and AV253706.2.
            
            On Feb 19, 2015 this sequence version replaced XM_006520087.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: HQ856050.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-99                AK048961.1         2-100
            100-3756            HQ856050.1         1-3657
            3757-3757           AC133959.4         37582-37582
            3758-5496           HQ856050.1         3659-5397
            5497-5559           AV253706.2         606-668
            5560-5565           AC133959.4         85427-85432
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 4.24 cM"
     gene            1..5565
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /note="sperm flagellar 2"
                     /db_xref="GeneID:320277"
                     /db_xref="MGI:MGI:2443727"
     exon            1..157
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             100..5496
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /note="isoform 1 is encoded by transcript variant 1; sperm
                     flagellar protein 2"
                     /codon_start=1
                     /product="sperm flagellar protein 2 isoform 1"
                     /protein_id="NP_001291971.1"
                     /db_xref="GeneID:320277"
                     /db_xref="MGI:MGI:2443727"
                     /translation="MSEILCQWLNQELRVSRTVSPKSFAKAFSNGYLIGEVLFKFELQ
                     SDFAEFSESRGSTAKLNNFSRLQPTLHLLGLQFDQNVAQSIITEKPGAATKLLYQLYI
                     ALQKKKKTGLTGLEIQTMQPQTNLRLQTLKSEAFRERLKNLIPRQTDFNLMRVTCRFQ
                     EKCKQMKEDLARMNFEKFEKIQKLEEEQRHFNIEKQRLNRRRQNEIMAKIQAAIIQIP
                     KPESNRTLKAIEAQKLMKKKKEAEDVANEIKKFEALIKKDLQIKESVSKTSLETTDQT
                     TAELLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLMAHEAQEEAYREEQL
                     IHRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQFEERRLKDFQDALDREAALA
                     KQAKIDFAEQTLREKEIHEKISVERAQQRYKKHYGICAEILDQMLDLCTKVADYRLLT
                     NNLIPHKMMHDWKELFFSGIPIYEQTSLTHGQTEPTEDHRAEVKKRNLLDSKDYEDYK
                     NMVGEWALPEDMVNSSPPSNNSILGHVLLRLIEKADPSASNAEATELPSLAVKGCILG
                     KTLSGKTTVLKSLQNDFPVHVLSIDTLVQEAIQAFHERQKSGKTPPTQEDDKRDPVVN
                     QEKVSKTQDKNVLAVSPVPGDRTSQKEGVKINEFEQFRSSDSFLSLSMRAQLGAKSEL
                     MLRRGKSIPDILLVSILVNAIKEIPVDQSWVLDGFPITLNQAKLLEEALTGYKRKFLQ
                     LKKKKEQMPTLALDPSTSTEVSLLPSALDFVILLDISDNSSLARTNDIIAKEISHEIS
                     HENVGRPGTGTSQDNKSEDRNLRDHIQHRIVGFLDNWPLLEEWFTQPKNILTKVNAEI
                     DEALLCQKVKEIFATETVNKKIKVEKTLEEKETEKKAGAPPAEPPAMSPPLSSEAEKD
                     KELHQAKTPGKGKTQSETQHGKQESQPEGKGKKVSPKGKAQGGKVSVKKSPVGSAEVS
                     PTPTAPPPPKAGTEEWVYVNEPIPEELPSFLVPYWELIEKSYINHIKTVLRHLRERQH
                     NVLSYLYETRTSFQEFLRRPDHKQDFVSQWQADFNSVPEDLWEDEETKAELHQRVNDL
                     RDRLWDICEARKEEAEQERLDIINESWLQDSIGITMNHFFSLMQAEVNRFQDTKRLLQ
                     DYYRAMESKIPLEDSKKFTRVPLVQLDGKEISESQLRIPLVPRISNSPENSAVKPKVG
                     TFLKGRSDPPLEVLEANFEIDEKILLDTWQQASLAISNMVAAEVHQRLTEEEKEPPQL
                     DSKEKSPQSGANKKAKKEKEAPKKKKTDKKGKGKSSPVAEVSPVTVTPEEMAEMEKRN
                     ELRLRIKEEHLAALQTEEQAAQFRLELIKLKALSVLEDLVTKVIDVYRLMEKWLGKRY
                     LNEMASIQKLTELARYHIETATKIQNEIYLSQEDFYINGDIKVFPDPSPPTRPPPVER
                     EENGTLTIEQLDNLRDQFLDMAPKGIIGNKAFSDILLDLITLNLGTNNFPSSWMHLSQ
                     LDLQEITSLLTVNTEFVDWRKFLMVTAMPWPMALEDELLDTLQRFKALDEAQTGTITY
                     EQYKQAGLWFSGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADCEKEPPQLDYTQML
                     LYFACHPDTLEGVYRALSVAVGTHIFRQVETPMLMAEKTSISTVSPIEEFPETEESSA
                     KEDRELKEEKDDQKEEEIPENANTEKISMETLLKVFGGGNEVLDANRFASYLKSENIY
                     AENFIKTFQDLGARNLEPIAVNILLKHPYIQDLIANYVDYKFPDIKMILQRSEHAQGS
                     DGERSPSRLTDEKK"
     exon            158..260
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            261..513
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            514..684
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            685..825
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            826..890
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1077
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1266
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1454
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1623
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1843
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..1995
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2053
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2054..2106
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2210
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2211..2467
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2468..2579
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2580..2737
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2738..2890
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2891..2941
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2942..3090
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3091..3218
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3219..3357
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3358..3495
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3496..3647
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3648..3828
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3829..3982
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3983..4111
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4112..4250
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4251..4480
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4481..4587
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4588..4770
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4771..4863
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4864..5043
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5044..5283
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5284..5406
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            5407..5565
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5542..5547
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5565
                     /gene="Spef2"
                     /gene_synonym="C230086A09Rik"
                     /note="major polyA site"
ORIGIN      
        1 agcaactggt tgccctgggc gacgggaaga cgcagtccag ctgactgagt tcctggaaag
       61 ttcctagcac ctgcaagcag tcaaattcac cagtgagcta tgtcggaaat cctgtgccag
      121 tggctcaacc aggagttgag ggtgtcccgg accgtgagtc ccaagtcatt tgcaaaggca
      181 ttttctaatg gctatctaat tggagaagtt ctgttcaagt ttgaacttca gagtgatttt
      241 gcagaatttt cagagagcag gggttcaaca gccaagctta acaatttctc ccgcttgcaa
      301 ccaacacttc acctgctggg tttgcagttt gatcagaatg tggctcaaag catcatcaca
      361 gagaagcctg gggcagcaac caaacttctg tatcagttgt acattgctct tcagaaaaag
      421 aagaaaacag ggctgactgg actagagata caaaccatgc aaccccagac aaacttgagg
      481 cttcagaccc tcaaaagtga ggcttttcga gagagactta aaaacctgat accacggcaa
      541 accgatttca atctgatgcg ggttacatgc aggtttcaag aaaagtgtaa acaaatgaaa
      601 gaagatcttg cccgaatgaa ttttgagaag tttgaaaaaa ttcaaaaact tgaggaagag
      661 caaagacact ttaatatcga aaagcaacgc ttaaacagaa gacgacaaaa tgagataatg
      721 gccaaaatcc aggcagccat tatacagatc cccaagcctg aatccaaccg tactttgaaa
      781 gctattgagg ctcaaaaact gatgaaaaag aaaaaggagg cagaggacgt ggccaatgag
      841 attaagaaat ttgaagcact aattaaaaag gatctccaaa ttaaagaaag tgtatccaag
      901 acttctttag aaacaacaga ccagacaact gcagaactgc tcaacactta ttcagatgat
      961 gactacatta aaaagatcca gaaacgccta gaagaagacg ctttcgcacg cgagcaaagg
     1021 gaaaaaagac gacgcagatt gttgatggat cagctaatgg cccatgaagc acaggaggag
     1081 gcctatcggg aagagcagct cattcaccgg ctcatgcgcc agtcccagca ggagcgcagg
     1141 attgctgtgc agctcatgca tgttcgccac gagaaggaag ttttgtggca aaatagaatt
     1201 ttccgagaga aacaatttga agaaagacga cttaaggatt tccaggatgc tcttgataga
     1261 gaagcggctc ttgccaaaca agccaagatt gattttgcag aacaaacctt gagggagaag
     1321 gaaattcatg aaaagatctc tgtggaaaga gcccaacagc gttataagaa gcattatggg
     1381 atatgtgcag agattttgga tcaaatgcta gatttgtgca cgaaggtggc agactatagg
     1441 ttgctgacaa ataatcttat tccacataag atgatgcacg attggaaaga actgtttttt
     1501 agtggaattc ccatatatga acagacttct cttacacatg gacaaacaga acccactgag
     1561 gatcaccgtg cagaagtgaa gaaaaggaac ttactcgata gcaaagatta tgaagattat
     1621 aagaacatgg ttggagagtg ggccttacca gaggacatgg ttaatagttc accaccctcc
     1681 aacaatagca ttttgggtca cgtgcttctg agattaattg aaaaagctga tccatctgca
     1741 agtaatgctg aagccactga attaccttca cttgcggtca agggttgtat actggggaag
     1801 acactcagtg gaaaaactac tgttctaaag tctctacaaa atgactttcc tgttcatgtg
     1861 ctttctattg atactcttgt ccaagaggct atccaggcgt ttcatgaaag acaaaaaagc
     1921 ggtaagacac caccaactca ggaagatgat aaaagagatc ctgtagtgaa ccaggaaaaa
     1981 gtgagcaaaa ctcaggataa aaatgtgtta gcagttagtc cagttccagg ggacagaaca
     2041 tcccaaaaag aaggtgtcaa gattaatgaa tttgaacagt tcagatcaag tgatagcttt
     2101 ttatcactta gcatgcgagc tcaacttggt gcaaagtcag agttgatgct tagaagagga
     2161 aagagcattc cagacatact tctggtcagc atcctggtaa atgctataaa ggagatacct
     2221 gtggaccaaa gctgggttct tgatggattt ccaataacat taaatcaagc caaactcctg
     2281 gaggaagcac ttacagggta caaaagaaaa ttcctacagt taaagaagaa gaaagaacaa
     2341 atgcccacat tggccctaga cccctcaact tccacagagg tctctcttct cccatctgca
     2401 cttgattttg tcatattgct agatatttcc gataactcat cgttggctcg aactaatgat
     2461 atcatagcta aagaaatctc acatgaaatt tctcatgaaa atgttggccg tcctggaact
     2521 ggtacaagcc aagataataa gagcgaggac cggaacttaa gagaccacat acagcacaga
     2581 attgttggct tcttggacaa ctggccactg ttggaagagt ggtttacaca gccaaaaaac
     2641 attctgacga aagtcaatgc tgaaatagat gaagcgctct tatgccaaaa agtaaaagaa
     2701 atttttgcta ctgaaacagt aaacaaaaag atcaaagttg aaaagacact ggaagaaaag
     2761 gaaactgaga aaaaggcagg agctcccccg gctgagcctc ccgctatgag ccctcccctt
     2821 tcttctgaag cagaaaaaga caaggagctt catcaggcaa aaactcctgg gaaaggaaag
     2881 acacaatcag aaacccagca tggtaagcaa gaatctcaac ctgaaggaaa aggaaagaaa
     2941 gtttctccta aaggaaaagc acaaggaggc aaagtttcag taaagaaatc accggttggc
     3001 tcagcagaag tatcacccac accaacagca ccaccgccac ctaaggcagg aacagaagaa
     3061 tgggtttatg tgaatgagcc tataccggag gaactccctt catttttggt accttactgg
     3121 gaactgatag agaaatccta catcaaccac atcaaaacag tacttcgaca tctcagagag
     3181 agacagcaca atgtgctctc ttatttatat gagactagga caagtttcca ggagtttcta
     3241 cggcgtccag atcacaagca agattttgta tctcagtggc aggctgattt caactctgtt
     3301 cctgaagacc tgtgggaaga tgaggaaaca aaggctgagc tacaccaaag agtaaatgat
     3361 ctgcgagacc gcctatggga catctgtgaa gcgaggaagg aagaggcgga acaggagagg
     3421 cttgatatca ttaatgagag ctggctgcag gactctatcg gaatcacaat gaatcatttc
     3481 ttttccctga tgcaggcaga agtgaaccgt tttcaagata ccaaaagact ccttcaagat
     3541 tattacagag caatggaatc caaaatccct ctggaagaca gtaagaaatt tactcgggtc
     3601 ccattggttc agcttgatgg taaagaaatt tcagaaagcc agctcagaat tcctctagtt
     3661 cctcgaatat ccaattctcc agaaaattct gccgtcaagc caaaggtggg gacatttctg
     3721 aagggcagat ctgatcctcc tttagaagtc ctggaagcca actttgaaat agatgagaag
     3781 attttattgg acacctggca gcaggcttca ttagccatct ctaacatggt ggctgctgaa
     3841 gttcaccaaa ggctcacaga ggaagaaaaa gaaccacccc aattagattc caaagaaaag
     3901 tctcctcagt caggtgcaaa taaaaaagcc aagaaggaga aagaagcacc caagaagaaa
     3961 aagacagaca agaaaggaaa aggcaaatca tcccccgtgg cagaagtcag tccagtcaca
     4021 gtcacacctg aggaaatggc tgagatggaa aagaggaatg aactgaggct caggataaag
     4081 gaagaacatc ttgctgccct gcagacagaa gaacaagccg cacaatttcg acttgagcta
     4141 ataaagttga aagctttgtc cgtcctggaa gatttagtaa caaaggtgat tgatgtctac
     4201 aggctcatgg aaaaatggct tggtaagaga tatttgaatg aaatggccag tatacagaaa
     4261 ttgaccgaat tagctcgcta ccacattgaa acagctacaa aaattcaaaa tgagatttac
     4321 ttaagccaag aagacttcta tatcaatgga gacataaaag tcttcccaga tccttctcca
     4381 ccaacccgcc caccaccagt agaaagggaa gaaaatggta ccctaacaat cgaacagctt
     4441 gataatcttc gagatcagtt cttagacatg gcacctaaag gcataatcgg aaataaagcc
     4501 ttcagtgaca ttctgcttga tttgattacc ctaaaccttg gaaccaataa cttccctagc
     4561 agctggatgc acctcagcca actggacttg caggagatca catctttatt aacagtcaac
     4621 acagagttcg tggactggag gaagttcttg atggtcacag ccatgccgtg gcccatggcc
     4681 ctggaggatg agcttctgga caccctgcag aggtttaagg ccctggatga agcacagaca
     4741 ggcaccatca cctacgaaca gtataagcag gctggtctat ggttttcagg agatgaagat
     4801 ataaaaattc cagaaaatcc ccttgaaccc ttgccattca atcgacagga gcatcttata
     4861 gagttcttct ttaggctgtt tgcggactgt gagaaggagc cacctcagct tgactacact
     4921 cagatgctgc tgtattttgc gtgccaccca gacactttgg aaggagtcta cagggccctc
     4981 agtgtggctg tgggaactca catcttccgc caggttgaaa ctccgatgct gatggcagaa
     5041 aagacctcca tctccactgt gagtcccatt gaggaattcc ctgagactga ggaaagtagt
     5101 gcaaaagagg accgggaact gaaggaggag aaagatgacc aaaaggaaga agaaattccc
     5161 gaaaatgcaa acactgaaaa gatttccatg gaaacactgc tcaaagtgtt tggaggagga
     5221 aatgaagtac tggatgccaa cagatttgcc agctacctaa aatcagagaa tatttatgca
     5281 gagaatttca tcaagacttt tcaggacctg ggtgccagga acctggagcc aattgcagtt
     5341 aatattctct tgaagcatcc ttacattcag gacctgattg caaattatgt agactataaa
     5401 tttcctgaca ttaaaatgat tcttcaaagg agtgagcatg cacaaggcag tgatggagaa
     5461 aggtcaccct caagacttac agacgaaaag aagtgaaggc tgtggttttg gttttttttg
     5521 ggtttttatt tgttgttgtt gaataaatgt tcataatcaa attga
//
