LOCUS       NM_027641               2264 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus sperm flagellar 1 (Spef1), mRNA.
ACCESSION   NM_027641 XM_918270 XM_988628
VERSION     NM_027641.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Zheng,J., Liu,H., Zhu,L., Chen,Y., Zhao,H., Zhang,W., Li,F.,
            Xie,L., Yan,X. and Zhu,X.
  TITLE     Microtubule-bundling protein Spef1 enables mammalian ciliary
            central apparatus formation
  JOURNAL   J Mol Cell Biol 11 (1), 67-77 (2019)
   PUBMED   30535028
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 2264)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2264)
  AUTHORS   Dougherty,G.W., Adler,H.J., Rzadzinska,A., Gimona,M., Tomita,Y.,
            Lattig,M.C., Merritt,R.C. Jr. and Kachar,B.
  TITLE     CLAMP, a novel microtubule-associated protein with EB-type calponin
            homology
  JOURNAL   Cell Motil Cytoskeleton 62 (3), 141-156 (2005)
   PUBMED   16206169
  REMARK    GeneRIF: a.k.a. Spef1 (sperm flagella) and CLAMP (CaLponin-homology
            And Microtubule associated Protein) CLAMP/Spef1 expression also in
            brain, lung, and inner ear MT association of CLAMP requires the CH
            domain, which has highest similarity with EB family proteins
            Erratum:[Cell Motil Cytoskeleton. 2005 Dec;62(4):259]
REFERENCE   5  (bases 1 to 2264)
  AUTHORS   Chan,S.W., Fowler,K.J., Choo,K.H. and Kalitsis,P.
  TITLE     Spef1, a conserved novel testis protein found in mouse sperm
            flagella
  JOURNAL   Gene 353 (2), 189-199 (2005)
   PUBMED   15979255
  REMARK    GeneRIF: demonstrate a specific pattern of Spef1 localisation in
            the seminiferous epithelium of adult mouse testis
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK047675.1 and AK046964.1.
            
            On Sep 16, 2006 this sequence version replaced NM_027641.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK047675.1, SRR1660815.208702.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164138
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1166              AK047675.1         2-1167
            1167-2264           AK046964.1         1233-2330
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 63.28 cM"
     gene            1..2264
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /note="sperm flagellar 1"
                     /db_xref="GeneID:70997"
                     /db_xref="MGI:MGI:3513546"
     exon            1..246
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /inference="alignment:Splign:2.1.0"
     CDS             138..842
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /note="calponin-homology and microtubule-associated
                     protein"
                     /codon_start=1
                     /product="sperm flagellar protein 1"
                     /protein_id="NP_081917.1"
                     /db_xref="CCDS:CCDS38245.1"
                     /db_xref="GeneID:70997"
                     /db_xref="MGI:MGI:3513546"
                     /translation="MESSVDEEALHQLYLWVDNIPLSRPKRNLSRDFSDGVLVAELIK
                     FYFPKMVEMHNYVPANSLQQKLSNWGHLNRKVLNKLNFSVPDDVMRKIAQCSPGVVEL
                     VLIPLRQRLEERQRRQKLGVGSLQELAPQDSSGYMDMGLPQKVRGEGAPALGEQLREG
                     RPLASRPPGYNQALQGDPSFVLQIAEKEQELLASQETVQVLQMKVKRLEHLLQLKNVR
                     IDDLSRRLQQAERKQR"
     exon            247..358
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /inference="alignment:Splign:2.1.0"
     exon            359..515
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /inference="alignment:Splign:2.1.0"
     exon            516..555
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /inference="alignment:Splign:2.1.0"
     exon            556..610
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /inference="alignment:Splign:2.1.0"
     exon            611..734
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /inference="alignment:Splign:2.1.0"
     exon            735..2264
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1466..1471
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /note="hexamer: ATTAAA"
     polyA_site      1488
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /note="major polyA site"
     regulatory      2188..2193
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
                     /note="hexamer: ATTAAA"
     polyA_site      2216
                     /gene="Spef1"
                     /gene_synonym="4931426K16Rik; Clamp"
ORIGIN      
        1 agaagggatt tgtgtctgtg ccgaataggg acccaaacgt atatctgaac gctcgcagtt
       61 gcgagaggag gcatttgaaa ggtcctgggg ctggcgctgg gctctgtcgg gaacgttgag
      121 gccttgctct ggatccaatg gaaagcagcg tggatgagga ggctctgcac cagctgtacc
      181 tctgggtaga caatatccct ctgtccagac ccaagagaaa cctctcccgg gacttcagtg
      241 acggagtcct ggttgcagag cttatcaagt tttacttccc caagatggtg gagatgcaca
      301 attatgtccc tgccaactct ctccagcaga agctcagcaa ctggggtcac ctgaacagaa
      361 aggtgctgaa caagctgaac ttctcggtcc ctgacgatgt gatgcggaag attgcgcagt
      421 gttctccggg agtagtggag ctagtgctca ttccactgag gcagcgcctg gaggagcggc
      481 agaggcgcca gaagctgggt gttggctcct tacaggagct ggctcctcag gatagcagtg
      541 gctacatgga tatgggtttg ccccagaagg ttcgaggaga gggtgcccca gccttgggag
      601 agcagctcag agagggccgt ccgttggctt cccgaccccc tgggtataac caggcgctgc
      661 agggtgatcc gagcttcgtc ctccagatcg ctgaaaagga gcaggagctg ttggcctctc
      721 aagagacagt acaggtcctt cagatgaagg tgaagcgtct ggaacatctg ctccaactta
      781 agaacgtgcg catcgatgat ctctcccggc ggctccagca ggccgagcgc aagcagcggt
      841 gagcggtgcc agggctgcgc cgggcttccc agtacccgcc cgagcccaga cgccgcgacc
      901 cacccactca cctgccgacg cagggttggg agggcggagc gagctgctca ctagtgcctc
      961 ctgcagctac ggcgctcggc tgcggagggt tcgagcgaac gtggggttgc tcaacgccga
     1021 ggccggggac tgatcctcct ccttacactc catctgggcc aggaaaaagg atcactttcc
     1081 acagcccaga aagcctgtac atcgcatgat ctcccaaacc actttgaagc tcccggcgct
     1141 gggcctggag aggcggtcct ctgtctcgtt ccactcctct agaaccggtt cctttctcag
     1201 catagaagat agcttcatct ctatccttgc taaatctcca aggcctccga cactcgagaa
     1261 attatcccgg gaagctgagg aattctcttg cccatcctga gtgctgaccg gggaccagtt
     1321 ttagggctgg aaatcacagc agctggagag gaagggataa ggtgttgaag taggcgggag
     1381 taagggaggc acccagctgt gcagtcgctc tcctctcacg gagcattcta aggagtgcct
     1441 gggaagatag ggcagagttg tctccattaa aggtgctgtt gtgcgttatt tcctgtgcct
     1501 gccttctctt tctgcctgta tggatcccca aaggcagtga gttcctggct tgtcctttat
     1561 ccctttcgcc cagctgtcct cttttagacc ctgtgggcct attattacat tgatgataat
     1621 gccagtggga agattttgtg acgtgtgaca gcctgcttct agcactgggt gaccccttcc
     1681 ttgtctgtcc gtccccaccc ctcagtgtcc tctaatcttg ttcaaaccca agctacgatt
     1741 ttcaccaatt gtggccactc atgtcctcaa ccttgcccat ggactctctt aaattagctt
     1801 tctgtatgac tctcaaccct gtccacacac tggtctcaat ttatggatgg ttggctacat
     1861 gtcacttgac aggcctggca actccattca taggcatatt acatgggaga aactcttgtg
     1921 ccctgagttc tgggagatac actgtaacaa tcatacagaa ttgaagtgat gggctggaga
     1981 gatggcttag ccgttaagag catttgctgt tcttacagag gacacaggtt ccatgcccag
     2041 cacccacatg acagctcaca actgttgatc actccaattc cagggtctcc aacaccctcc
     2101 tctacctcca taaactccag gactgcatgt ggtgcacaga gagatatatg agcttaaaat
     2161 aactctaaaa actttaaaaa aaaaagaatt aaaaggaact tgtatttggt tatctattac
     2221 tgtgtacaaa ttaccatcaa tgtaaaggct taaagcaaca catg
//
