LOCUS       NM_153406               6078 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus sperm antigen with calponin homology and coiled-coil
            domains 1-like (Specc1l), transcript variant 2, mRNA.
ACCESSION   NM_153406
VERSION     NM_153406.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Saadi,I., Goering,J.P., Hufft-Martinez,B.M. and Tran,P.V.
  TITLE     SPECC1L: a cytoskeletal protein that regulates embryonic tissue
            dynamics
  JOURNAL   Biochem Soc Trans 51 (3), 949-958 (2023)
   PUBMED   37345651
  REMARK    GeneRIF: SPECC1L: a cytoskeletal protein that regulates embryonic
            tissue dynamics.
REFERENCE   3  (bases 1 to 6078)
  AUTHORS   Bertol,J.W., Johnston,S., Ahmed,R., Xie,V.K., Hubka,K.M., Cruz,L.,
            Nitschke,L., Stetsiv,M., Goering,J.P., Nistor,P., Lowell,S.,
            Hoskens,H., Claes,P., Weinberg,S.M., Saadi,I., Farach-Carson,M.C.
            and Fakhouri,W.D.
  TITLE     TWIST1 interacts with beta/delta-catenins during neural tube
            development and regulates fate transition in cranial neural crest
            cells
  JOURNAL   Development 149 (15) (2022)
   PUBMED   35781329
REFERENCE   4  (bases 1 to 6078)
  AUTHORS   Goering,J.P., Wenger,L.W., Stetsiv,M., Moedritzer,M., Hall,E.G.,
            Isai,D.G., Jack,B.M., Umar,Z., Rickabaugh,M.K., Czirok,A. and
            Saadi,I.
  TITLE     In-frame deletion of SPECC1L microtubule association domain results
            in gain-of-function phenotypes affecting embryonic tissue movement
            and fusion events
  JOURNAL   Hum Mol Genet 31 (1), 18-31 (2021)
   PUBMED   34302166
  REMARK    GeneRIF: In-frame deletion of SPECC1L microtubule association
            domain results in gain-of-function phenotypes affecting embryonic
            tissue movement and fusion events.
REFERENCE   5  (bases 1 to 6078)
  AUTHORS   Goering,J.P., Isai,D.G., Hall,E.G., Wilson,N.R., Kosa,E.,
            Wenger,L.W., Umar,Z., Yousaf,A., Czirok,A. and Saadi,I.
  TITLE     SPECC1L-deficient primary mouse embryonic palatal mesenchyme cells
            show speed and directionality defects
  JOURNAL   Sci Rep 11 (1), 1452 (2021)
   PUBMED   33446878
  REMARK    GeneRIF: SPECC1L-deficient primary mouse embryonic palatal
            mesenchyme cells show speed and directionality defects.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6078)
  AUTHORS   Wilson,N.R., Olm-Shipman,A.J., Acevedo,D.S., Palaniyandi,K.,
            Hall,E.G., Kosa,E., Stumpff,K.M., Smith,G.J., Pitstick,L.,
            Liao,E.C., Bjork,B.C., Czirok,A. and Saadi,I.
  TITLE     SPECC1L deficiency results in increased adherens junction stability
            and reduced cranial neural crest cell delamination
  JOURNAL   Sci Rep 6, 17735 (2016)
   PUBMED   26787558
  REMARK    GeneRIF: SPECC1L as a novel modulator of PI3K-AKT signaling and AJ
            biology, required for neural tube closure and CNCC delamination.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6078)
  AUTHORS   Saadi,I., Alkuraya,F.S., Gisselbrecht,S.S., Goessling,W.,
            Cavallesco,R., Turbe-Doan,A., Petrin,A.L., Harris,J., Siddiqui,U.,
            Grix,A.W. Jr., Hove,H.D., Leboulch,P., Glover,T.W., Morton,C.C.,
            Richieri-Costa,A., Murray,J.C., Erickson,R.P. and Maas,R.L.
  TITLE     Deficiency of the cytoskeletal protein SPECC1L leads to oblique
            facial clefting
  JOURNAL   Am J Hum Genet 89 (1), 44-55 (2011)
   PUBMED   21703590
REFERENCE   8  (bases 1 to 6078)
  AUTHORS   Endoh-Yamagami,S., Karkar,K.M., May,S.R., Cobos,I., Thwin,M.T.,
            Long,J.E., Ashique,A.M., Zarbalis,K., Rubenstein,J.L. and
            Peterson,A.S.
  TITLE     A mutation in the pericentrin gene causes abnormal interneuron
            migration to the olfactory bulb in mice
  JOURNAL   Dev Biol 340 (1), 41-53 (2010)
   PUBMED   20096683
REFERENCE   9  (bases 1 to 6078)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 6078)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Hara,Y., Nagase,T.,
            Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: I. The complete nucleotide sequences of 100 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 9 (5), 179-188 (2002)
   PUBMED   12465718
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY884297.1, AK041543.1, BC072595.1 and AI853253.1.
            
            On Mar 12, 2009 this sequence version replaced NM_153406.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and uses a downstream
            translational start codon, compared to variant 1. The resulting
            isoform (b) is shorter at the N-terminus, compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.183653.1,
                                           SRR1660817.88982.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1881              AY884297.1         2-1882
            1882-2534           AK041543.1         375-1027
            2535-4725           AY884297.1         2536-4726
            4726-6055           BC072595.1         4565-5894
            6056-6078           AI853253.1         1-23                c
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 38.51 cM"
     gene            1..6078
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /note="sperm antigen with calponin homology and
                     coiled-coil domains 1-like"
                     /db_xref="GeneID:74392"
                     /db_xref="MGI:MGI:1921642"
     exon            1..147
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            148..337
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     CDS             185..3541
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /note="isoform b is encoded by transcript variant 2;
                     cytospin-A; SPECC1-like protein"
                     /codon_start=1
                     /product="cytospin-A isoform b"
                     /protein_id="NP_700455.3"
                     /db_xref="CCDS:CCDS48599.1"
                     /db_xref="GeneID:74392"
                     /db_xref="MGI:MGI:1921642"
                     /translation="MKKANRSAGSVPKVSGISKPQTVEKSKPENSSSAPTGVKPVRPG
                     AAAALSKTKSNDDLLAGMAGGVNVTNGIKAKKSTCSSAAPSAPAPAMTISENKSKIST
                     GTSSSAKRSTSAGNKESSSTRERLRERTRLNQSKKLPSVSQGANDVALAKRSRSRTAA
                     EGDIRMSKSKSDNQISDKAALEAKVKDLLTLAKTKDVEILHLRNELRDMRAQLGISED
                     HCEGEDRSEVKETIIAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALG
                     FSLEQRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTL
                     MDHQHSNSMDNLDSECSEVYQPLTSSDDALDAPSSSESEGVPSIERSRKGSSGNASEV
                     SVACLTERIHQMEENQHSTSEELQATLQELADLQQITQELNSENERLGEEKVILMESL
                     CQQSDKLEHFGRQIEYFRSLLDEHHISYVIDEDVKSGRYMELEQRYMDLAENARFERE
                     QLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAATLEEY
                     KATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLA
                     SSLQEDLAHTRNDANRLQDTIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSELEEKDT
                     ERSDMKETIFELEDEVEQHRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRL
                     REESAEWRQFQADLQTAVVIANDIKSEAQEEIGDLKRRLHEAQEKNEKLTKELEEIKS
                     RKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPTVKTLIKSFDSASQVP
                     NAAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRSNYGELPV
                     QEHLLRTSSTSRPASLPRVPAMESAKTISVSRRSSEEMKRDISASEGASPASLMAMGT
                     TSPQLSLSSSPTASVTPSTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQ
                     NIDITNFSSSWNDGLAFCALLHTYLPAHIPYQELNSQDKKRNFTLAFQAAESVGIKST
                     LDINEMARTERPDWQNVMLYVTAIYKYFET"
     exon            338..491
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            492..2125
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2333
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            2334..2407
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2583
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            2584..2747
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            2748..2839
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            2840..2930
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            2931..3014
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            3015..3171
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            3172..3274
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            3275..3391
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            3392..3451
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     exon            3452..6062
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6036..6041
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /note="hexamer: AATAAA"
     polyA_site      6055
                     /gene="Specc1l"
                     /gene_synonym="4930470P14Rik; 4932439K10Rik;
                     9530057A13Rik; Cytsa; mKIAA0376"
                     /note="major polyA site"
ORIGIN      
        1 aagggagaca gggcgggccg agggagcggc gccgtggggt tggctgttgc cctgaggccg
       61 gactcaacgg accgggccgc gccaccgggc gagctcgccg cccggcccca gcccggagcc
      121 agcgagtgag cgaggcggtg ccgcgcggtt tggttgtaaa tgcatcacca agaggcagcc
      181 cagaatgaag aaagcaaaca ggagtgctgg ctcagtgcct aaggtgtctg ggataagcaa
      241 accgcaaaca gtagagaaaa gcaagcctga gaacagctct tcagcaccca caggagtcaa
      301 gcctgtgagg cctggggcag cagcagcctt gtcaaagacc aagagcaatg atgacctttt
      361 agctggaatg gctggaggag taaacgtgac taatggtatt aaagcaaaga aaagcacctg
      421 ttcatctgca gcgccttcag cccccgcccc tgccatgacc atctcggaga acaagtccaa
      481 gatcagcaca ggcactagtt cctcagccaa acggagtact tcggctggta ataaagagtc
      541 cagttctact agagaaagat tacgtgaacg cacccggcta aaccagagca aaaaactgcc
      601 ctctgtaagt cagggagcta atgatgtggc actggctaaa cgttcacgca gccgcactgc
      661 tgcagagggt gatattcgta tgagcaagtc taagtcggac aatcagatca gtgacaaagc
      721 tgctttggaa gccaaggtga aagatctcct cacactggcg aaaaccaagg atgtggaaat
      781 tttacatttg agaaatgaac tccgagacat gcgtgctcag ctgggcatta gtgaggacca
      841 ttgtgaaggt gaggacaggt ctgaggtgaa ggaaactatt attgctcacc agcctactga
      901 cgtggagtct actctcctgc agctgcagga gcagaataca gccatccgcg aggagctcaa
      961 ccagctgaag aatgaaaaca gaatgctaaa ggacaggctc aatgctttgg gcttttccct
     1021 agagcaaagg ctggacaatt ctgaaaaact atttggctat cagtccctga gcccagaaat
     1081 cacccctggt aaccagagtg acggaggagg aactctgaca tcttcggtag aaggttctgc
     1141 tcctgggtcg gtggaagatc tcctgagtca ggatgaaaat acactcatgg accatcagca
     1201 cagtaactcc atggacaacc tggacagtga gtgcagcgag gtgtaccagc ccctcacttc
     1261 cagcgatgat gctctggacg ccccctcctc ttcagagtca gagggcgtgc caagcattga
     1321 gcgctctcgg aaggggagca gtgggaatgc cagtgaggtg tccgtcgctt gcctgacaga
     1381 acggattcac cagatggaag agaaccagca cagcaccagt gaggagcttc aggccactct
     1441 acaggagctg gctgatctcc agcagattac ccaggagctc aacagtgaga acgaaaggct
     1501 gggagaggag aaggtcattc tgatggagtc gctgtgtcag cagagtgata agttggaaca
     1561 ctttggccgg cagattgagt atttccgttc ccttcttgat gagcatcaca tttcctatgt
     1621 catagatgaa gacgtaaaga gcgggcgcta tatggaacta gagcagcgct acatggattt
     1681 ggctgagaat gcccgttttg agcgagagca gcttctaggt gtgcagcagc atttaagcaa
     1741 cactttgaag atggcagaac aagataacaa agaagctcaa gaaatgatag gtgccctcaa
     1801 agagcgcagc caccacatgg agcggattat tgagtctgag cagaagggca aagcggccct
     1861 ggcagccaca ttggaggagt acaaagctac tgtggccagt gaccagatag agatgaatcg
     1921 cttgaaggcc cagctggaga acgaaaagca gaaggtggct gagttgtatt ctatccataa
     1981 ctctggagac aagtccgata tccaggatct cctggagagt gtcaggctgg acaaggaaaa
     2041 ggcggagact ttggccagta gcctgcagga agatctggct catacccgaa atgatgccaa
     2101 tcggttacag gacaccattg ctaaggtaga agatgaatac cgagctttcc aggaagaggc
     2161 caagaagcag attgaagatt tgaatatgac attagaaaaa ctaagatcag agttggagga
     2221 aaaggacacg gagaggagtg acatgaagga aaccatcttt gaacttgaag atgaggtgga
     2281 gcagcaccgc gccgtgaagc tgcatgacaa cctcattatc tctgatctag agaatacagt
     2341 taagaaactc caagaccaaa agcatgacat ggaaagagaa atcaagacac ttcacaggag
     2401 acttcgggaa gagtctgcag aatggcggca atttcaggct gatctccaga ctgcagtggt
     2461 cattgccaat gatatcaagt ctgaagccca agaagagatc ggtgatctaa agcgacggct
     2521 acatgaggcc caagagaaaa atgagaaact cacaaaagaa ttggaggaaa taaagtcacg
     2581 caagcaagag gaagagcgtg gccgggtgta taactacatg aatgctgttg aaagagattt
     2641 ggcagccttg aggcaaggga tgggactgag tagaaggtcc tccacttcct cagaaccaac
     2701 tcccacagta aaaaccctca tcaagtcttt cgacagtgca tctcaagtac caaacgctgc
     2761 tgcagctgcg atccctcgaa cacctctgag tccaagtccc atgaaaaccc ctcctgcagc
     2821 tgccgtttct cccatgcaga gacattccat aagtggtcca atctcaacct ccaagcctct
     2881 gacagccttg tcagataaga gatcaaacta tggagagctt cctgtacaag agcatctact
     2941 aagaacatct tcaaccagcc ggcccgcctc tctcccaaga gtccctgcta tggaaagtgc
     3001 caagaccatc tcagtgtctc gacggagtag tgaagaaatg aaacgggaca tctctgcatc
     3061 tgagggcgcg tcaccagcct ctctcatggc tatgggcacc acgtcaccac agctctccct
     3121 ctcttcctct cccactgcat ctgtcactcc aagcacccgg agccgaataa gggaagaaag
     3181 gaaagacccc ctctcagcat tggcaagaga atatggaggc tccaagagga acgccttgtt
     3241 gaagtggtgt cagaagaaaa cagaaggcta tcagaatatt gacatcacaa acttcagcag
     3301 cagctggaac gacgggctgg ctttctgcgc attgcttcat acctatctcc cagcccacat
     3361 accatatcaa gaactgaaca gtcaagataa gaaaaggaac ttcaccctgg ctttccaggc
     3421 agctgaaagt gttggaatca aatcgacgct ggacatcaat gaaatggcgc gtactgaacg
     3481 gcccgactgg cagaacgtca tgctgtatgt tacagccatc tacaagtact ttgaaacctg
     3541 agcacacagg agcggctgat accagctagg gacctctgca gtcaccaagt gacaccaaca
     3601 ccattagcta gaactcctaa agctttcagt gactctgggt ctccctgcag ggagtgtgcc
     3661 cagctctagg cctctgcatt ggaactggaa ctgcaggcca gggctcttcc gcatggccgt
     3721 cctcaggagc agaagtacag cacaccgcca gcctacttcc agagaccgtc cggctccacc
     3781 agggccctgg agtgcaaaca gcataaaggc tgtgcccggc cgggcttatc ttgggaaatg
     3841 ggattaagtt gtgggaacca tgggttagtg aaagctttag ccatccaact tcttgactat
     3901 tatccacgtg attcccagtg gtgggacaga gctggctttg gtcccttcat gtgggcctgg
     3961 gttctaggag tgtgtcagag gaaagtgtca gtacccgttc catggagagg accgtcctaa
     4021 ccactgcact tagcttagga gtgttaaaac cctgcttata ggcccacagc tcgttgagtc
     4081 tacactgcca ccaagtaggt ttttgtcagc agcaagacgc aggtggcaca ctcagtgggt
     4141 gctcagccac ttgcctgtaa caccagggaa aagactggtc acaaggagtt ctcctcattg
     4201 aagttgttgt tttattataa gcaatattgg tgaaatagct cctgttagta ccaaagttaa
     4261 tcgtttcagt aagcatagaa ctaaaacaca gactgggcat tagcatgtga gagggagggt
     4321 tcgtgcagta gggaaagggg ggtgctttag ggagccagag attcaagttg tactggtggc
     4381 tgcccctcca gggcaggagt taagacagtg ccttagccct gcaccaggac agctgagcct
     4441 acacccacat gaggatcctg tgtgctgatt gggactccag gagaagtggc tctacgctgt
     4501 gcctgggctg tggggcctat atcacgagcc aaggccttac ccggtttgtt gatttctggc
     4561 tggaatttag cagcctcttt tcgagaatag gaagccctta tttcatgtgc ctgacacaga
     4621 agctgttgtt tgaacaatgt caagacctgt tgctccggga tacacttgtt tgtcacctgc
     4681 aaggcaccat gtggaagact ggccatcaat aaaaatgggg ccacagcttc agtaacaaga
     4741 tggatggtgg gaatgtctcc caggaggcat gccaggccaa acatggactg agtgaggttc
     4801 tgtattcaag cataatgcta cagactagga atctgttgta tctatggaac gtagagaagg
     4861 aagcaatttg aaagtagtta ttgggaagtg ggccgtgtct gtttgtgttg ccatcagatg
     4921 gccagtccca cccctccacc ctgcttaaat gtcccaatct ggcagcttat gctgcagagt
     4981 gtaccaggag accctattat tctgtctgcc cagtgcctct ctggccctcc ttggcccagt
     5041 tctgttcttg ttcttgttct gctgatgaac tccgttgggg tgaccattgt gtgaaaatga
     5101 ctgtttccaa atgagtctcc actgaactcc tctgagcttc cagcctctgc tgtatttgcc
     5161 ttcactgagg ccttccactg tgcaccaagg tggtggatct gaggccaggt tttatttatt
     5221 atcatgaaaa ggggttgtct tgttagagac tttcctgggc ttgcagttca caccttctgt
     5281 cccctgctgg ctatgtgatc ctcatggcag tgtggaagtt gcccgagttt tctgaaagga
     5341 tttggagtaa gtcagctgct gctgaacgag tatgtccatg tagaggtaac attctgtttt
     5401 ggaatctgtg ggcctgggct aggcctggag ccagtgcagg gacagacaga tggtgggcta
     5461 gaccctctca catgcacaca cagactgggt gtctcacagc cagggccagg ctgcccacat
     5521 ttgtgtgagg gtccacagtc aagtgctgtc ttcagaccac tgctttttgc cttctctaat
     5581 aggccactaa agagcactgt tgaccctctg ggcatgcttg tagatggcca tgtgctatta
     5641 aaagtagtat ttgggagttc ttagagtgcc tgtctacaat gcacgaagcc ctgggttgat
     5701 ccctaccacc acatacactg agtgtggtgc atacctgtca ttctagtact tggggaaacg
     5761 gaggcaggag gatcatgagt tcagtgttac tgttgattac acggtgagtt tgatgtcagc
     5821 cctggcatgt gagaccctgt ccgccaaaag gtgatatttg gaggttttct tgttctgggg
     5881 ctgtttcctg ggaagctcta agatgtaagc tcgcctcttc ctaaagcaca agcttcgcta
     5941 caaaagggcc agctaccttt ctactgttgt cagtcacagg cttctgccga tgaatgacac
     6001 tttgtcattt tgttctagta aatactcttg aatgtaataa actgtcatgc gtcgatttcc
     6061 taaaaaaaaa aaaaaaaa
//
