LOCUS       NM_025668               2818 bp    mRNA    linear   ROD 14-JUN-2024
DEFINITION  Mus musculus signal peptidase complex subunit 2 homolog (S.
            cerevisiae) (Spcs2), transcript variant 1, mRNA.
ACCESSION   NM_025668
VERSION     NM_025668.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   3  (bases 1 to 2818)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113298.14 and AC115040.7.
            
            On Mar 23, 2023 this sequence version replaced NM_025668.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK134578.1, SRR13422600.2070482.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-122               AC113298.14        163565-163686
            123-206             AC115040.7         168779-168862       c
            207-367             AC115040.7         168102-168262       c
            368-502             AC115040.7         164503-164637       c
            503-2818            AC115040.7         157371-159686       c
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 54.15 cM"
     gene            1..2818
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
                     /note="signal peptidase complex subunit 2 homolog (S.
                     cerevisiae)"
                     /db_xref="GeneID:66624"
                     /db_xref="MGI:MGI:1913874"
     exon            1..122
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
                     /inference="alignment:Splign:2.1.0"
     CDS             9..689
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
                     /EC_number="3.4.-.-"
                     /note="isoform 1 is encoded by transcript variant 1; SPase
                     25 kDa subunit; microsomal signal peptidase 25 kDa
                     subunit"
                     /codon_start=1
                     /product="signal peptidase complex subunit 2 isoform 1"
                     /protein_id="NP_079944.1"
                     /db_xref="CCDS:CCDS40033.1"
                     /db_xref="GeneID:66624"
                     /db_xref="MGI:MGI:1913874"
                     /translation="MAASASQGGRSGGGGGSSGAGGGPSCGTSSSRSGLLDKWKIDDK
                     PVKIDKWDGSAVKNSLDDSAKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVALIWD
                     YMHPFPESKPVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQLS
                     SSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYEPEISRLHDS
                     LATERKIK"
     exon            123..206
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
                     /inference="alignment:Splign:2.1.0"
     exon            207..367
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
                     /inference="alignment:Splign:2.1.0"
     exon            368..502
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
                     /inference="alignment:Splign:2.1.0"
     exon            503..2818
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
                     /inference="alignment:Splign:2.1.0"
     regulatory      680..685
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
                     /note="hexamer: AATAAA"
     polyA_site      701
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
     regulatory      1464..1469
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
                     /note="hexamer: AATGAA"
     polyA_site      1483
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
                     /note="major polyA site"
     regulatory      2794..2799
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
                     /note="hexamer: AATAAA"
     polyA_site      2818
                     /gene="Spcs2"
                     /gene_synonym="5730406I15Rik; mKIAA0102"
ORIGIN      
        1 gagacaagat ggcagcgtcg gcgtcgcagg gtgggagaag cggcggtggc ggaggcagta
       61 gtggggctgg cggcggcccc agctgcggga caagcagcag ccgcagtggt ttgttggata
      121 agtggaagat tgatgataag cctgtaaaaa ttgataagtg ggatggatca gctgtgaaga
      181 actctctgga tgactctgcc aaaaaggtac ttctggaaaa gtacaagtat gtggagaact
      241 ttggtctcat tgacggtcgc ctcaccatct gtaccatctc ctgctttttt gccatcgtgg
      301 ctttgatttg ggattacatg cacccctttc ctgagtccaa gccagttttg gctttgtgcg
      361 tcatatccta ttttgtgatg atggggattc tgaccattta tacctcatat aaagagaaga
      421 gcatcttcct tgtggcccac agaaaagacc ccacaggaat ggatcctgat gatatttggc
      481 agctgtcctc cagcctcaaa aggtttgatg acaaatacac cctgaagttg accttcatca
      541 gtgggaggac aaagcagcag cgggaagccg agttcaccaa gtccattgct aagttctttg
      601 accacagcgg gacattggtc atggatgcat atgaacctga aatatccagg ctccatgaca
      661 gtctcgccac agaaagaaaa ataaaatagc cacttgtaaa agctctcctt ctgctggatc
      721 atacgactca ctggtgggta ggggaggaga cagaaagctt aagcttggtc aagtaagaaa
      781 tgttaaagat gcccctgtct aatccaccct ggatgaatag agttttctag ttcagatatg
      841 cagttgtccc tttgatggct gtagcctccc tggtctgctg agtggttttc tgttgctttc
      901 cttaagcgtt tttgtaagag ctaagcatat ttttcctttg tgcttaatag tactttatgc
      961 agtctagctt tgtgccatgc agcgtttgaa tgctcagttc ttaagaacta ctaaccttgc
     1021 tggggcacag tagtgttcac ccagaatccc agcacttccc aggctgagga agggggaatg
     1081 atgtgaggcc atcctgggct gtagagccag gccctggctt ggggagtggg cattgttaac
     1141 ttgttgctga ctttgtgttg acccctgcat cagcaactat ttccttaaat ccaggataca
     1201 acttgttaag tgtgacagct ttcctttaca caccattttt gtgggtgtat atatatattt
     1261 gacttgggga gaattatttt ttacaaaaat acaaaatagc tttttaattt gtttaaaata
     1321 gacctgcctg ttactttttg tgctcttaac caattaaaga agccggtggc attttttagt
     1381 tttatattct ctgttttcca ctcgtatgtt cctgttgatt tgtgcccttt attaactgcc
     1441 attttctaaa atttttttca ataaatgaaa gaagatgtga aaatgtggag tcttgaaaga
     1501 aggaacagca cacacagcac gtgctctact tgggtggagt cccctctagg ctggctcact
     1561 gtttgtgggc ccttgagaat tcggagccag gggcttagtt ctagtgatag cagtgacact
     1621 gggcacatca catgcattgg ctcgctgaag ctcacccaca acagtcctaa ggcaggcact
     1681 gttgtcctca cacaggagga agcttaggca tgaaagttga caggccacca gatgttatgt
     1741 tccttcagag acaaatttct caacttggtc agagaactgg gcagtttgag gagtctaatt
     1801 ctaagtctag taccccaaat tatattccct atatgaaagc atccagagag catccagaat
     1861 gttgtcctgt ctagcactac acagtgagac cacactgcca gatgttgaag gcttggattc
     1921 tgtaagccta taagcctgcc tataatcctt cgctaagtca gttccttgag cttctaatgc
     1981 tgcccccacc ctttcttgta aaagcaacag tgcagcgctg agaaggtggc tcacttaata
     2041 cagtgctggt gtgcaagcat gggatcccta gtttggcctt gagaatgcat gtgaagcgaa
     2101 gccagctgct agagttgtaa tccctctgct ggtgacatgg gtacagggtg ctctctagca
     2161 tcctagccag ccagcctcaa agccagagct ggggagatga ctgggagggt gaaatgcttg
     2221 ctgtgtaagc agcggcgtct gagtttggat ccccagaacc cacatagaag ccatgcatgg
     2281 tgaagcacat ctaacccttg tgctagggaa caaatcaagg cagaggctag ggtcttgctg
     2341 gcagccagtc tggctgaatt agcaagctct ggtgcagtaa aagccctggt ctcagaagtc
     2401 aggctgcata gaaatggaag acctgtactg tcagccgctg gcctccacag gtgcatagac
     2461 tgataatcta ggtcagcctc tgacctctgt gcgtgggcac acaggcacgt acacacaggg
     2521 taaaggttca agttcagtga cccacttaaa ggttttgaaa gtgtttgatt agataatgac
     2581 actggcacag ggacagtcac tattgttagg gaggttccag tgctaggaga ggaggccgtg
     2641 gttaccgcac agctgctcta ccctttgctt ccctttgctt tatgtctata caaatatatc
     2701 cttggctttg ggtttgtact tgttaggcaa atgttctaac actgacctac tcctccagct
     2761 ctccaaacac agatttatat atgcgccccc ttaaataaac actccaacta tgctggca
//
