LOCUS       NM_001199124            1388 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus SPC25, NDC80 kinetochore complex component, homolog
            (S. cerevisiae) (Spc25), transcript variant 3, mRNA.
ACCESSION   NM_001199124
VERSION     NM_001199124.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Zhang,J., Liu,W., Li,G., Xu,C., Nie,X., Qin,D., Wang,Q., Lu,X.,
            Liu,J. and Li,L.
  TITLE     BCAS2 is involved in alternative splicing and mouse oocyte
            development
  JOURNAL   FASEB J 36 (2), e22128 (2022)
   PUBMED   34972250
REFERENCE   3  (bases 1 to 1388)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1388)
  AUTHORS   Ulmke,P.A., Sakib,M.S., Ditte,P., Sokpor,G., Kerimoglu,C., Pham,L.,
            Xie,Y., Mao,X., Rosenbusch,J., Teichmann,U., Nguyen,H.P.,
            Fischer,A., Eichele,G., Staiger,J.F. and Tuoc,T.
  TITLE     Molecular Profiling Reveals Involvement of ESCO2 in Intermediate
            Progenitor Cell Maintenance in the Developing Mouse Cortex
  JOURNAL   Stem Cell Reports 16 (4), 968-984 (2021)
   PUBMED   33798452
REFERENCE   5  (bases 1 to 1388)
  AUTHORS   Naiman,N., Fujioka,K., Fujino,M., Valerius,M.T., Potter,S.S.,
            McMahon,A.P. and Kobayashi,A.
  TITLE     Repression of Interstitial Identity in Nephron Progenitor Cells by
            Pax2 Establishes the Nephron-Interstitium Boundary during Kidney
            Development
  JOURNAL   Dev Cell 41 (4), 349-365 (2017)
   PUBMED   28535371
REFERENCE   6  (bases 1 to 1388)
  AUTHORS   Foley,E.A. and Kapoor,T.M.
  TITLE     Microtubule attachment and spindle assembly checkpoint signalling
            at the kinetochore
  JOURNAL   Nat Rev Mol Cell Biol 14 (1), 25-37 (2013)
   PUBMED   23258294
  REMARK    Review article
REFERENCE   7  (bases 1 to 1388)
  AUTHORS   Machold,R., Klein,C. and Fishell,G.
  TITLE     Genes expressed in Atoh1 neuronal lineages arising from the
            r1/isthmus rhombic lip
  JOURNAL   Gene Expr Patterns 11 (5-6), 349-359 (2011)
   PUBMED   21440680
REFERENCE   8  (bases 1 to 1388)
  AUTHORS   Sun,S.C., Lee,S.E., Xu,Y.N. and Kim,N.H.
  TITLE     Perturbation of Spc25 expression affects meiotic spindle
            organization, chromosome alignment and spindle assembly checkpoint
            in mouse oocytes
  JOURNAL   Cell Cycle 9 (22), 4552-4559 (2010)
   PUBMED   21084868
  REMARK    GeneRIF: Spc25 is required for chromosome alignment, spindle
            formation, and proper spindle checkpoint signaling during meiosis.
REFERENCE   9  (bases 1 to 1388)
  AUTHORS   Ciferri,C., Pasqualato,S., Screpanti,E., Varetti,G., Santaguida,S.,
            Dos Reis,G., Maiolica,A., Polka,J., De Luca,J.G., De Wulf,P.,
            Salek,M., Rappsilber,J., Moores,C.A., Salmon,E.D. and Musacchio,A.
  TITLE     Implications for kinetochore-microtubule attachment from the
            structure of an engineered Ndc80 complex
  JOURNAL   Cell 133 (3), 427-439 (2008)
   PUBMED   18455984
REFERENCE   10 (bases 1 to 1388)
  AUTHORS   Trimarchi,J.M., Stadler,M.B. and Cepko,C.L.
  TITLE     Individual retinal progenitor cells display extensive heterogeneity
            of gene expression
  JOURNAL   PLoS One 3 (2), e1588 (2008)
   PUBMED   18270576
  REMARK    Publication Status: Online-Only
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL929221.6, CJ086993.1,
            CX222514.1 and BC027121.1.
            
            On Apr 3, 2015 this sequence version replaced NM_001199124.1.
            
            Summary: This gene encodes a component of the
            kinetochore-associated NDC80 protein complex, which is required for
            the mitotic spindle checkpoint and for microtubule-kinetochore
            attachment. During meiosis in mouse, the protein localizes to the
            germinal vesicle and then is associated with the chromosomes
            following germinal vesicle breakdown. Knockdown of this gene in
            oocytes results in precocious polar body extrusion, chromosome
            misalignment and aberrant spindle formation. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Apr
            2015].
            
            Transcript Variant: This variant (3) uses an alternate splice site
            in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode
            the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027121.1, AK151554.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 AL929221.6         160089-160093       c
            6-23                CJ086993.1         6-23
            24-82               CX222514.1         1-59
            83-1388             BC027121.1         1-1306
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 39.64 cM"
     gene            1..1388
                     /gene="Spc25"
                     /gene_synonym="2600017H08Rik; 2610205L13Rik; Spbc25"
                     /note="SPC25, NDC80 kinetochore complex component, homolog
                     (S. cerevisiae)"
                     /db_xref="GeneID:66442"
                     /db_xref="MGI:MGI:1913692"
     exon            1..150
                     /gene="Spc25"
                     /gene_synonym="2600017H08Rik; 2610205L13Rik; Spbc25"
                     /inference="alignment:Splign:2.1.0"
     exon            151..303
                     /gene="Spc25"
                     /gene_synonym="2600017H08Rik; 2610205L13Rik; Spbc25"
                     /inference="alignment:Splign:2.1.0"
     exon            304..369
                     /gene="Spc25"
                     /gene_synonym="2600017H08Rik; 2610205L13Rik; Spbc25"
                     /inference="alignment:Splign:2.1.0"
     exon            370..516
                     /gene="Spc25"
                     /gene_synonym="2600017H08Rik; 2610205L13Rik; Spbc25"
                     /inference="alignment:Splign:2.1.0"
     exon            517..621
                     /gene="Spc25"
                     /gene_synonym="2600017H08Rik; 2610205L13Rik; Spbc25"
                     /inference="alignment:Splign:2.1.0"
     exon            622..720
                     /gene="Spc25"
                     /gene_synonym="2600017H08Rik; 2610205L13Rik; Spbc25"
                     /inference="alignment:Splign:2.1.0"
     exon            721..1371
                     /gene="Spc25"
                     /gene_synonym="2600017H08Rik; 2610205L13Rik; Spbc25"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1348..1353
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spc25"
                     /gene_synonym="2600017H08Rik; 2610205L13Rik; Spbc25"
                     /note="hexamer: AATAAA"
     polyA_site      1371
                     /gene="Spc25"
                     /gene_synonym="2600017H08Rik; 2610205L13Rik; Spbc25"
                     /note="major polyA site"
ORIGIN      
        1 ggcgggtaag caggcggtgg agagtttgaa attgaaagac tgcgcgctgg aggagcagca
       61 gacccgggag cagcagacgg aagagcggcg ggctggttcc tgccggacag gtccagtctg
      121 gtggagaccg cggcgttcct ggccgctcag tgtcttaaaa cataatgggt gaagacgaat
      181 tggcactctt aaatcaaagc atcaatgaat ttggggataa gttcagaaac agactcgatg
      241 ataatcatag tcaagtgctg ggacttagag acgccttcaa ggactccatg aaagcgtttt
      301 cagaaaagat gtctttgaaa ttaaaggaag aagagaggat gactgagatg attctggagt
      361 ataaaaacca gctctgtaag cagaataagc tcattcaaga aaagaaagag aatgtgttga
      421 agatgattgc tgaagtaaaa ggcaaggagc aagagtcgga agagctgact gctaaaatcc
      481 aggagctcaa ggaagagtac gctaggaaga gggaaaccat ttccactgct aacaaagcta
      541 atgaagagag attgaaagga ctgcagaaat cagcggatct gtatagagat taccttggac
      601 tagaaattag aaagattcac ggtaataaat tgcagtttat atttactagt attgacccta
      661 agaatcctga gagcccatat atgttttcca tgagcataaa tgaagctaag gaatatgaag
      721 tgtacgacag ttcgcctcat cttgagtgcc tagcagaatt tcaggagaaa gtcaggaaga
      781 ccaacaattt ttcagctttt cttgccaata ttcggaaggc ttttatagct aaggttcata
      841 attgatatag gtttgttctt tgttactgta tatatccaga ggaattctca tctcttgtcc
      901 ctgtcatttt tctctctttc tgtgtggtat taaagaaact agaatatatc atcccaaacc
      961 atgcctcttt gaggtaacat ttgtgagctg aaaacagtta agcagcagca gagttagaaa
     1021 acctttgcct tcccccagtc tacctaaaca tgggacctag atgatagagg tatctagctc
     1081 tgtacccaca aagaaagata gaagttagtc gcttaccaca gcagacgtgg caccagagga
     1141 attgacaagc cttgcttcat taatttattc ctgtaaatat attcttcaca attccccatc
     1201 tgtggaagtc aaaaggcaaa tctgtctgtc ttatcatgtt gtaaaatcct actgttctct
     1261 gttgaagatg ctgtgtaaac ctgactaggc cactgtttgg agttccttct gttggggttc
     1321 tcctgtattg taggctctgc ttgtgttaat aaacttgctt gttgtctccc aaaaaaaaaa
     1381 aaaaaaaa
//
