LOCUS       NM_001418664            3024 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus spermatogenesis associated, serine-rich 2 (Spats2),
            transcript variant 2, mRNA.
ACCESSION   NM_001418664
VERSION     NM_001418664.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   3  (bases 1 to 3024)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   4  (bases 1 to 3024)
  AUTHORS   Senoo,M., Hoshino,S., Mochida,N., Matsumura,Y. and Habu,S.
  TITLE     Identification of a novel protein p59(scr), which is expressed at
            specific stages of mouse spermatogenesis
  JOURNAL   Biochem Biophys Res Commun 292 (4), 992-998 (2002)
   PUBMED   11944913
REFERENCE   5  (bases 1 to 3024)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC157610.2 and AC161198.5.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR12282455.15425718.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849384
                                           [ECO:0000350]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               AC157610.2         1669-1804           c
            137-260             AC157610.2         834-957             c
            261-557             AC161198.5         61781-62077
            558-637             AC161198.5         79227-79306
            638-746             AC161198.5         85549-85657
            747-796             AC161198.5         86549-86598
            797-1055            AC161198.5         90454-90712
            1056-1235           AC161198.5         92728-92907
            1236-1371           AC161198.5         98106-98241
            1372-1430           AC161198.5         111355-111413
            1431-1540           AC161198.5         117745-117854
            1541-1643           AC161198.5         120980-121082
            1644-1858           AC161198.5         123029-123243
            1859-3024           AC161198.5         124197-125362
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 56.01 cM"
     gene            1..3024
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /note="spermatogenesis associated, serine-rich 2"
                     /db_xref="GeneID:72572"
                     /db_xref="MGI:MGI:1919822"
     exon            1..136
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            137..260
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            261..557
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     CDS             533..2170
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /note="isoform 1 is encoded by transcript variant 2;
                     serine-rich spermatocytes and round spermatid protein,
                     59kDa; spermatogenesis-associated serine-rich protein 2;
                     serine-rich spermatocytes and round spermatid 59 kDa
                     protein"
                     /codon_start=1
                     /product="spermatogenesis-associated serine-rich protein 2
                     isoform 1"
                     /protein_id="NP_001405593.1"
                     /db_xref="GeneID:72572"
                     /db_xref="MGI:MGI:1919822"
                     /translation="MSRKQSQKDSSGFIFDLQSNTVLAQGGTFENMKEKINAVRAIVP
                     NKSNNEIILVLQHFDNCVDKTVQAFMEGSASEVLKEWIVTGKKKNKKKKSKPKPASEA
                     SGSAPDSSKSAPIQEEQPASSEKGSINGYHVNGAINDAESVDSLSEGLETLSIDAREL
                     EDPEFAAAETLDRTGSVLENGVSDFEPKSLTAHSISNVQQSRNAAKSLSRTTPGAQVS
                     NLGMENVPLSSTNKKLGSNIEKSVKDLQRCTVSLARYRVVVKEEMDASIKKMKQAFAE
                     LQSCLMDREVALLAEMDKVKAEAMEILLSRQKKAELLKKMTDVAVRMSEEQLVELRAD
                     IKHFVSERKYDEDLGRVARFTCDVETLKQSIDSFGQVSHPKNSYSTRSRCSLVAPVSL
                     SGPSDGSAASSSPDASVPSLPGANKRNCAPREASAAMTNSSDRPCQAHREVFPGNRRG
                     GQGYRAQSQKTADPSNPGRHDSVGRYRNSSWYSSGPRYQGVPPQAPGNAGERSRPYSA
                     GTNGTGAISEPSPPKPSFKKGLPQRKPRASQAEAANS"
     exon            558..637
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            638..746
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            747..796
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            797..1055
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1235
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1371
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1430
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1540
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1643
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            1644..1858
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..3024
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3001..3006
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /note="hexamer: AATAAA"
     polyA_site      3024
                     /gene="Spats2"
                     /gene_synonym="2700012F11Rik; 59kDa; p59; p59scr; Scr59"
                     /note="major polyA site"
ORIGIN      
        1 agcgacggcg gcggctcccg aggacgaggg cgaggaggag gcggaggctc ttctttcctc
       61 atcccagacc cgggagcgtc cggaacgcag agcccggaac tggggggacg cggcggctgc
      121 agggagcccg ggctagctca gcagactact gtatataatt tctccgtatt tattgaagca
      181 agagagaaca cactgccggc agtactctac catgagcgag gtcagcgata ctgcggagtt
      241 gaagatgttt ggtttgaaaa gatctccctg gggaaagaca tgactgtctg cggagattca
      301 tcctgacaag aagttgatac cagagaagga aaagaaattg acagctagag aacagaatct
      361 taaacaacca ggattttgga tttatcactt ccaactatag acttctattg ccacttttaa
      421 attagcagga ttcccagaac ttggacgaag ataatttctt ctggtttttg cttgttttct
      481 ctctttcttt tcttttttct ttctttttta atttgatcgt gaggaaactg caatgtctag
      541 gaaacaaagc cagaaagatt catcaggatt catttttgat ttgcagtcca atactgtcct
      601 ggcccaggga ggaacctttg agaatatgaa ggagaagata aacgcggtac gtgcgatagt
      661 tcccaataag agcaacaatg agatcatcct ggtcttgcag cactttgaca actgtgtgga
      721 caaaacagtg caagcgttca tggaaggtag tgccagtgaa gtgctcaaag aatggatagt
      781 aactggcaaa aaaaagaaca aaaagaagaa gagcaagcca aagcctgcat cagaagcaag
      841 tggcagtgcc ccagactcca gtaagtcggc ccccattcaa gaggagcagc ctgcatcttc
      901 agagaaaggc agcatcaatg gttaccacgt aaacggtgcc atcaacgatg ccgagtctgt
      961 ggactcgctc agtgaagggc tggagacgct gtcaatagat gccagagagc tggaggatcc
     1021 cgagtttgcc gcagcagaga cgctggacag aactggatct gtgctggaga atggtgtctc
     1081 tgactttgag cccaaatctt tgactgcgca ctctatctcg aatgtccagc aatcccggaa
     1141 cgctgccaaa tctctgtcca gaaccacccc aggagcacag gtttcaaatc tgggaatgga
     1201 gaacgttcca ctctcttcca ccaataaaaa gctaggttct aatattgaga aatcggtgaa
     1261 agacctccag cgctgcactg tttctcttgc acggtaccga gttgtcgtta aagaagagat
     1321 ggatgcgtcc attaagaaaa tgaaacaagc gtttgccgaa ttgcaaagct gtttaatgga
     1381 tcgagaagtg gcactgcttg ctgagatgga taaagtgaaa gctgaagcaa tggaaatctt
     1441 gctcagccgg cagaagaaag cggagcttct aaagaagatg acagatgtgg ccgttcgcat
     1501 gtcagaggag cagttggttg aactcagggc tgatatcaag cactttgtca gtgagcggaa
     1561 atatgatgaa gaccttggaa gggtagcccg cttcacctgt gatgtggaga ctctgaaaca
     1621 gagtatcgat tctttcggac aagtgtcaca tccaaagaac agctactcaa cccgctctcg
     1681 atgcagcttg gtggcacctg tgtccttgag tggcccaagt gatggctctg ctgcttcttc
     1741 ctcccctgat gcctctgttc ccagcctccc aggtgctaac aagagaaact gtgccccaag
     1801 agaggcctct gcagccatga cgaactccag tgaccggccc tgccaggctc atcgggaggt
     1861 atttccaggc aacaggagag gaggacaagg ctaccgggca caaagccaaa agaccgcaga
     1921 cccctcaaac ccagggcgcc atgacagtgt gggccgctac agaaacagct catggtattc
     1981 ttccgggccc aggtatcagg gtgttccgcc acaagcccca ggaaacgccg gcgagcggag
     2041 tcgcccttac tctgcaggga ccaatggaac tggagccatc tcagagccta gcccacccaa
     2101 gccttcgttc aaaaaggggc tcccacagcg caaaccaagg gcctctcagg ctgaagctgc
     2161 gaactcctga ggggaattcc agttagcctc tcttctctcc catccagttc aacctaataa
     2221 ctggacttta ggaaatgtat acttagattt aataacaaaa agaaatttat acatatctct
     2281 ttgggccatc ctaaatattt ttgatttctt ctctcctttt tttttttttt ttgaatgaaa
     2341 gctgttttcc cttggcctct cctagtgata tggactgact ctggttggtc gttttcacca
     2401 ggaaccaggg gaactattgc cagttttccc aaaggtgagc agcctccttc ccagcgcacc
     2461 tcacctccag ctgcttaccg cagaggctcc gggaggaaga gtgtctgcta gtccagtctc
     2521 cagtccattc gagcagatgc cacaggtgcc tgccaggttc ccaccagttg cctgagcggg
     2581 agtagaaatg aggggtttta tacactactg ggtcagccag aaaagggaaa tgtgcgtcag
     2641 ttcgtgaaag agcccgggga acgccctctg tctggaatgt gcctgaggtc tgacatcaag
     2701 tcagggtttc catcccacac ttggcaccgt accttaacct gcagtttctt ccaaatgccc
     2761 aaagccttgt ttccgcttac cttaggctga aacccggcct aaggaaatct atgggaaagt
     2821 aacattttaa gttttttaaa agaaaaaatg tttttctttg tttcagtgtt tggagatttt
     2881 tgttgtttgt tgtccctccc tttcctgaat cctgttatga accaatttta tataatataa
     2941 ttagtgttaa tctaaggtgt tactcggcac cgttgttgcg gtcatcttta taagtgaata
     3001 aataaaagct agtgcatcac gtga
//
