LOCUS       NM_001289574            2137 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus spermatogenesis associated 7 (Spata7), transcript
            variant 4, mRNA.
ACCESSION   NM_001289574 XM_006515360
VERSION     NM_001289574.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Lu,J., Xiong,K., Qian,X., Choi,J., Shim,Y.K., Burnett,J., Mardon,G.
            and Chen,R.
  TITLE     Spata7 is required for maintenance of the retinal connecting cilium
  JOURNAL   Sci Rep 12 (1), 5575 (2022)
   PUBMED   35368022
  REMARK    GeneRIF: Spata7 is required for maintenance of the retinal
            connecting cilium.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2137)
  AUTHORS   Collin,G.B., Gogna,N., Chang,B., Damkham,N., Pinkney,J., Hyde,L.F.,
            Stone,L., Naggert,J.K., Nishina,P.M. and Krebs,M.P.
  TITLE     Mouse Models of Inherited Retinal Degeneration with Photoreceptor
            Cell Loss
  JOURNAL   Cells 9 (4), 931 (2020)
   PUBMED   32290105
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2137)
  AUTHORS   Dharmat,R., Eblimit,A., Robichaux,M.A., Zhang,Z., Nguyen,T.T.,
            Jung,S.Y., He,F., Jain,A., Li,Y., Qin,J., Overbeek,P., Roepman,R.,
            Mardon,G., Wensel,T.G. and Chen,R.
  TITLE     SPATA7 maintains a novel photoreceptor-specific zone in the distal
            connecting cilium
  JOURNAL   J Cell Biol 217 (8), 2851-2865 (2018)
   PUBMED   29899041
  REMARK    GeneRIF: The absence of Spata7 results in the mislocalization of
            DCC proteins without affecting the proximal connecting cilium (PCC)
            protein complexes.
REFERENCE   5  (bases 1 to 2137)
  AUTHORS   Kasowitz,S.D., Ma,J., Anderson,S.J., Leu,N.A., Xu,Y., Gregory,B.D.,
            Schultz,R.M. and Wang,P.J.
  TITLE     Nuclear m6A reader YTHDC1 regulates alternative polyadenylation and
            splicing during mouse oocyte development
  JOURNAL   PLoS Genet 14 (5), e1007412 (2018)
   PUBMED   29799838
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2137)
  AUTHORS   Eblimit,A., Agrawal,S.A., Thomas,K., Anastassov,I.A., Abulikemu,T.,
            Moayedi,Y., Mardon,G. and Chen,R.
  TITLE     Conditional loss of Spata7 in photoreceptors causes progressive
            retinal degeneration in mice
  JOURNAL   Exp Eye Res 166, 120-130 (2018)
   PUBMED   29100828
  REMARK    Erratum:[Exp Eye Res. 2018 Jun;171:119. doi:
            10.1016/j.exer.2018.03.011.. PMID: 29579643]
REFERENCE   7  (bases 1 to 2137)
  AUTHORS   Eblimit,A., Nguyen,T.M., Chen,Y., Esteve-Rudd,J., Zhong,H.,
            Letteboer,S., Van Reeuwijk,J., Simons,D.L., Ding,Q., Wu,K.M.,
            Li,Y., Van Beersum,S., Moayedi,Y., Xu,H., Pickard,P., Wang,K.,
            Gan,L., Wu,S.M., Williams,D.S., Mardon,G., Roepman,R. and Chen,R.
  TITLE     Spata7 is a retinal ciliopathy gene critical for correct RPGRIP1
            localization and protein trafficking in the retina
  JOURNAL   Hum Mol Genet 24 (6), 1584-1601 (2015)
   PUBMED   25398945
REFERENCE   8  (bases 1 to 2137)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   9  (bases 1 to 2137)
  AUTHORS   Wang,H., den Hollander,A.I., Moayedi,Y., Abulimiti,A., Li,Y.,
            Collin,R.W., Hoyng,C.B., Lopez,I., Abboud,E.B., Al-Rajhi,A.A.,
            Bray,M., Lewis,R.A., Lupski,J.R., Mardon,G., Koenekoop,R.K. and
            Chen,R.
  TITLE     Mutations in SPATA7 cause Leber congenital amaurosis and juvenile
            retinitis pigmentosa
  JOURNAL   Am J Hum Genet 84 (3), 380-387 (2009)
   PUBMED   19268277
  REMARK    GeneRIF: Spata7 is expressed in the mature mouse retina.
            Erratum:[Am J Hum Genet. 2010 Feb;86(2):293. Abboud, Emad B
            [added]; Al-Rajhi, Ali A [added]]
REFERENCE   10 (bases 1 to 2137)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124516.4.
            
            On Jan 24, 2014 this sequence version replaced XM_006515360.1.
            
            Transcript Variant: This variant (4) contains an alternate exon and
            lacks an alternate exon in the 5' coding region, and uses an
            alternate downstream translation start codon, compared to variant
            1. The encoded protein (isoform 4) has a shorter and distinct
            N-terminus, compared to isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.120004.1,
                                           SRR7345562.3834826.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-193               AC124516.4         89405-89597         c
            194-268             AC124516.4         85702-85776         c
            269-430             AC124516.4         83588-83749         c
            431-478             AC124516.4         80126-80173         c
            479-612             AC124516.4         69257-69390         c
            613-1046            AC124516.4         59093-59526         c
            1047-1113           AC124516.4         58123-58189         c
            1114-1229           AC124516.4         55652-55767         c
            1230-1283           AC124516.4         54521-54574         c
            1284-1361           AC124516.4         53434-53511         c
            1362-1419           AC124516.4         48850-48907         c
            1420-2137           AC124516.4         47920-48637         c
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 49.88 cM"
     gene            1..2137
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /note="spermatogenesis associated 7"
                     /db_xref="GeneID:104871"
                     /db_xref="MGI:MGI:2144877"
     exon            1..193
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     exon            194..268
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     exon            269..430
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     exon            431..478
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     exon            479..612
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     CDS             559..1989
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /note="isoform 4 is encoded by transcript variant 4;
                     spermatogenesis-associated protein 7 homolog"
                     /codon_start=1
                     /product="spermatogenesis-associated protein 7 homolog
                     isoform 4"
                     /protein_id="NP_001276503.1"
                     /db_xref="GeneID:104871"
                     /db_xref="MGI:MGI:2144877"
                     /translation="MQTNSKMNSKFFVNSLQKPSGEPQDQDVFIEEMTRYPSFSKSLI
                     PSSEGLHLSLPESSKMLMSGTQKHASTSPSRHSGCGHGCDRRPRSAHQFQVALAKTPS
                     GDLLEKHSDLFSNKQSPFTPRTLKTEAKSFLSQYRYYTPAKRRKDFSDQRMEAETQTE
                     LSSFNSELGTAEKTSSKDSEVNINQVPNYTRNGAEDKIAPLPSQGQNLAWDSIQDGIL
                     QQSSERASCKLSTEFSPDSKIYSDEEELLYLSFMENVTDEILKLGLFSNRFLERLFER
                     HIKKNKHHLEEGKMRYLLHGLKVDLGCISEEDPAKQKHFRMLNQLHFQKALISRENEF
                     VSDEETVSHHERQQYQEALDMLSAVPKDENKMFSLPGEFLIPAHKVKHSEGVIIQQVN
                     DETDNEASPWNENNPSVSDSVIDQETSVDVIEGDSDFERAETSRELCCLSTSLSPSGP
                     FPSINGGSNHGKELSTLRIMGMSIED"
     exon            613..1046
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1113
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1229
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1283
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1361
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1419
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..2137
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2006..2011
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /note="hexamer: AATAAA"
     polyA_site      2035
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /note="major polyA site"
     regulatory      2103..2108
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
                     /note="hexamer: AATAAA"
     polyA_site      2133
                     /gene="Spata7"
                     /gene_synonym="B230306G18Rik; HSD3"
ORIGIN      
        1 ataatctgca gccgcggagc tcgcccttag caaccgccct ggcgactgtc gctgtgaccc
       61 tttgcgcccc cgcggtcctg gtcttttcct tttcacctcc gctgcggggg agctcggctt
      121 catcccagta agaaccaagg ggatacaacc gcggaggccg caggaggact aagcatggat
      181 ggcagccgga gagtcagagc aacctctgtc ctccccagat acagccctcc atgcttgttt
      241 acaggacacc tgagcaccaa gagcaacgaa tctcttcgca aactctcaag cttgtaaaac
      301 ttcactcttg aagaggctgg agcccaggtg atggctgctg ctgtcagtgt cggagactgg
      361 acacagagcc atgacagtgt gtgcattctc cgccgcctcc agcgaagtcc agaacgggac
      421 tgagaatgac ccgcagtgga ctgctcaatt ccagtaagtg tgaatactag catcaaatat
      481 gctgaccaac aacgaagaga aaaactgagg aaggagttgg cacggtgtga aaaggagttt
      541 aaattaagta aatctgctat gcagaccaat tctaagatga attccaagtt ctttgtaaat
      601 tctctacaga agccctcagg ggaaccacaa gaccaggatg tgttcataga agaaatgaca
      661 agatacccat cattttcaaa gtcactcatc ccttcttccg agggactgca tctaagtctg
      721 cctgaatcaa gtaagatgct catgagtggc acccagaagc atgccagcac ctctccatcc
      781 agacactcag gctgtgggca tggctgcgac aggcggcctc ggagtgccca ccagttccag
      841 gtagccctcg ccaagacccc aagtggagat ctgttggaaa agcactctga cctcttctct
      901 aacaagcagt cgccattcac tccacgaact ttaaaaacag aagcaaagtc cttcctgtca
      961 cagtaccgct actacacacc tgccaaaaga agaaaggatt tttcagatca gcggatggaa
     1021 gctgaaaccc aaactgaatt aagcagcttt aactctgagc ttgggacagc tgagaaaacg
     1081 agctcaaagg actcagaagt gaacataaac caggtaccta attacacgag aaatggtgct
     1141 gaagacaaaa tagctccttt accctcacaa ggacaaaact tagcatggga cagtattcaa
     1201 gatgggattc tgcagcagtc ctcagaaagg gcatcctgta agctctccac agagttttct
     1261 ccagacagta aaatctactc tgatgaagaa gagctgctgt acctgagttt catggaaaat
     1321 gtgacggatg aaatcttgaa acttggttta ttttctaaca ggtttctaga gcgactgttt
     1381 gagcgacata taaagaaaaa taaacatcat ttggaggagg gaaagatgcg ctacctgctg
     1441 catgggctga aggttgactt aggctgcata tctgaggaag acccagcaaa gcaaaaacat
     1501 ttcagaatgt tgaatcaact tcattttcaa aaggctctga tttcaagaga aaatgagttt
     1561 gtaagtgatg aagaaacagt gagtcaccac gagcgccagc agtaccagga ggccctggac
     1621 atgctgtcgg ctgtgccgaa ggacgaaaac aagatgttct ctttaccggg cgaattttta
     1681 atacccgccc ataaagtcaa gcattcagag ggtgttataa ttcaacaggt caatgacgaa
     1741 acagataatg aagcctcacc ctggaatgaa aacaatccaa gtgtttctga tagtgtaata
     1801 gaccaggaaa cctctgtgga tgtcattgaa ggtgacagtg actttgaaag ggctgagact
     1861 tccagggaac tctgttgtct gagcacatca ctgtccccat ctggtccatt ccccagcatc
     1921 aatggtggca gtaatcatgg taaggaatta tcgactctac gaatcatggg aatgagcatt
     1981 gaagactgac ctctggatct acattaataa acactccatg tggccagtaa ctcaatatta
     2041 cttttcttct ttttttttgt ccccttttac atattaagat ttctgtaatt tcaatgctat
     2101 gtaataaatt accacaattt tagtgtcttg aaacacc
//
