LOCUS       NM_026470               2454 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus spermatogenesis associated 6 (Spata6), transcript
            variant 1, mRNA.
ACCESSION   NM_026470 XM_981105
VERSION     NM_026470.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 2454)
  AUTHORS   Yuan,S., Stratton,C.J., Bao,J., Zheng,H., Bhetwal,B.P.,
            Yanagimachi,R. and Yan,W.
  TITLE     Spata6 is required for normal assembly of the sperm connecting
            piece and tight head-tail conjunction
  JOURNAL   Proc Natl Acad Sci U S A 112 (5), E430-E439 (2015)
   PUBMED   25605924
  REMARK    GeneRIF: Inactivation of Spata6 in mice leads to acephalic
            spermatozoa and male sterility. Our proteomic analyses reveal that
            SPATA6 is involved in myosin-based microfilament transport through
            interaction with myosin subunits
REFERENCE   4  (bases 1 to 2454)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 2454)
  AUTHORS   Oh,C., Aho,H., Shamsadin,R., Nayernia,K., Muller,C., Sancken,U.,
            Szpirer,C., Engel,W. and Adham,I.M.
  TITLE     Characterization, expression pattern and chromosomal localization
            of the spermatogenesis associated 6 gene (Spata6)
  JOURNAL   Mol Hum Reprod 9 (6), 321-330 (2003)
   PUBMED   12771232
REFERENCE   6  (bases 1 to 2454)
  AUTHORS   Chiellini,C., Costa,M., Novelli,S.E., Amri,E.Z., Benzi,L.,
            Bertacca,A., Cohen,P., Del Prato,S., Friedman,J.M. and Maffei,M.
  TITLE     Identification of cathepsin K as a novel marker of adiposity in
            white adipose tissue
  JOURNAL   J Cell Physiol 195 (2), 309-321 (2003)
   PUBMED   12652657
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK051574.1, AK153098.1, AK005819.1 and CK129415.1.
            
            On Jul 14, 2006 this sequence version replaced NM_026470.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132771.1, AK153098.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1291              AK051574.1         2-1292
            1292-1726           AK153098.1         1169-1603
            1727-2325           AK005819.1         1114-1712
            2326-2454           CK129415.1         1-129               c
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 51.53 cM"
     gene            1..2454
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /note="spermatogenesis associated 6"
                     /db_xref="GeneID:67946"
                     /db_xref="MGI:MGI:1915196"
     exon            1..248
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     CDS             198..1664
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; spermatogenesis-associated protein 6;
                     kinesin-related protein"
                     /codon_start=1
                     /product="spermatogenesis-associated protein 6 isoform 1
                     precursor"
                     /protein_id="NP_080746.3"
                     /db_xref="CCDS:CCDS38836.1"
                     /db_xref="GeneID:67946"
                     /db_xref="MGI:MGI:1915196"
                     /translation="MPKVKALQCALALEIRSVTCPGVVLKDKEDIYLSICVFGQYKKT
                     QCVPATFPLVFNARMVFEKVFPEAVDPGDVVAQLEYDTAVFELIQLVPPVGETLSTYD
                     ENTRDFMFPGPNQMSGHHDSNRQVTMRRISGLRGIAPKLEFSTTSVITECLISSRKCR
                     TQDKFTYHSAPVEKSHGRLQCRTSRSQKKKSKSPERSKYCINTKNYEQPTISSKSHSP
                     SPYTKRRMCELSEDTRRRLAHLNLGPYEFKKETDKPPFVIRHVDPPSPRTDNFFGSPG
                     RDCERDGWVRMHSDHPHIGCCRSKDYKVIRSPHGRDFEDPFERCEEYLSPRTCSKPQH
                     SARTLLVHSAPSTTPKHCASPVLNRASLRERFHSDWCSPPNCDEIHDRVKDVLKSHQA
                     HARHLCDERDPEREDELELKRSLLYRDSAYDSDPEYSSFQRPRGSFHLDDGECWSNRA
                     ASCKGKSHRPVFENSMDKMYRNLYKKACSSVSHTQESF"
     exon            249..386
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     exon            387..435
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     exon            436..477
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     exon            478..602
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     exon            603..683
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     exon            684..977
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1065
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1106
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1291
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1391
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1483
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..2435
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1708..1713
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /note="hexamer: AATGAA"
     polyA_site      1727
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /note="major polyA site"
     regulatory      2415..2420
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
                     /note="hexamer: AATAAA"
     polyA_site      2435
                     /gene="Spata6"
                     /gene_synonym="1700062C23Rik; Hash; KRP; Mash"
ORIGIN      
        1 atgacaacga gtccgcgccc cgcgctcagc tgctgttcgg gcgggaccca ggctggaccg
       61 cgggccccgg cctgggggcc acagctgcca cctccgccgc cacctcatct cctccctgtc
      121 cctcgtcccg ccccgcgtct tcccgccgta gtcgggccgg aggccggggt ccctccccgc
      181 cgccctaccc tgcgcggatg ccgaaggtga aggcgctgca atgcgccctg gcgctggaga
      241 tccgctctgt aacttgtcca ggagtggtgc tgaaagacaa ggaggacatc tatcttagta
      301 tctgtgtgtt tggccaatac aaaaagacac aatgtgtccc agccactttt cccctggtct
      361 tcaatgccag aatggtgttt gaaaaggtgt tcccggaagc agtagaccct ggagatgtgg
      421 tcgcacagct tgagtatgat acagcagtgt ttgagttgat acagctagtt ccaccagtgg
      481 gagaaacact gtctacctat gatgaaaata cacgagattt catgttcccg ggtccaaacc
      541 aaatgtctgg acaccatgat tcaaaccgcc aggttaccat gaggaggatt tctggcctaa
      601 gaggaattgc tccaaagctg gaattttcta caacttcagt gatcactgaa tgtctgataa
      661 gctcaaggaa gtgccgcact caggataaat ttacttacca ttcagctcca gttgaaaaat
      721 cccacggcag actgcaatgc agaacatcaa gatcacagaa gaaaaagtcc aagtcacccg
      781 agaggagtaa atattgcata aacaccaaga actacgaaca gcctacgatt tcctcaaagt
      841 cacactctcc atctccctac acaaaaagac ggatgtgtga gctttccgaa gacactcggc
      901 ggcggctggc ccatttaaat ctgggaccct atgaattcaa aaaggaaaca gataagcctc
      961 catttgtgat tagacatgtt gatcccccga gtcccagaac tgacaatttt tttggatcac
     1021 ctggcagaga ctgtgaacga gatggatggg tcaggatgca cagcgatcat cctcatattg
     1081 gctgctgcag atccaaggac tacaaggtta tcaggtcacc acatgggaga gactttgagg
     1141 atccctttga aaggtgtgag gaatatttga gcccaaggac atgtagcaag ccccagcatt
     1201 ctgcaaggac cttactagtc cattcagcac cttcaacaac accaaagcat tgtgcaagcc
     1261 ctgtgctaaa tagagcctct ctcagggaaa gattccactc tgattggtgt tcacctccga
     1321 actgtgatga gatccatgac cgggtaaaag atgtcttgaa atcacatcaa gctcatgcaa
     1381 gacatttatg tgatgagaga gacccagaga gagaagacga actggaactg aaaagaagtc
     1441 ttttatatag agactctgct tatgacagtg accctgagta tagctccttt cagcggccgc
     1501 gtggctcttt ccatttggat gatggcgaat gctggtccaa cagagcagcc tcttgtaaag
     1561 ggaaatccca ccgacccgtc tttgagaaca gcatggacaa gatgtacagg aacttataca
     1621 aaaaagcctg tagttctgtt tctcatacac aggaaagctt ctgagaccat ctaccataaa
     1681 ctgtataagc gtccatgtca acctctcaat gaaagcgttt gatgtgatct gtatttgtac
     1741 gcctttcttt aaaacagatg ttcactgagt aacagagttg aacccagcat atttcttcaa
     1801 gtggtaaatt atcacagtcc agcctttatt acagaagaat gtgctgtttc caatgtcaaa
     1861 actctataat attgtcacaa cttccaatct gtgacaaggc tatcttgcca catgtgctcc
     1921 tgtcaccatc gacaaactga agaataggtt ctttctagaa tattcttcca attttatacc
     1981 cttttaattt tccacctgct tggctggtta tcttatttgg ataatattta attagtatta
     2041 gcttcctgtt ctcaggaatg agcaaaaaga tgttctctgc agaaacaaat tttaataatt
     2101 taaacctgtt ctaatattat ctactttttg ctcttagaag aggctattgt atttcttggg
     2161 tcctaaaaac tggctttctt tgtaaagctc acatatgcac tcactccttc tttagaagac
     2221 cagctgagtt acctgaatga ctaacagata gatttgttca ctaggagaga ccatgaatgg
     2281 tcattcatgt agctaatatt ttagtgttgg attatttgca atactttctt ccagggaaat
     2341 acattttgat gcaaacagaa taatgaagca gcctgtgtta gtaagcatgt agaaactaaa
     2401 aataaataat tgaaaataaa agaattatat tgtaaaaaaa aaaaaaaaaa aaaa
//
