LOCUS       NM_133711               1057 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus spermatogenesis associated 4 (Spata4), mRNA.
ACCESSION   NM_133711
VERSION     NM_133711.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Miyata,H., Castaneda,J.M., Fujihara,Y., Yu,Z., Archambeault,D.R.,
            Isotani,A., Kiyozumi,D., Kriseman,M.L., Mashiko,D., Matsumura,T.,
            Matzuk,R.M., Mori,M., Noda,T., Oji,A., Okabe,M.,
            Prunskaite-Hyyrylainen,R., Ramirez-Solis,R., Satouh,Y., Zhang,Q.,
            Ikawa,M. and Matzuk,M.M.
  TITLE     Genome engineering uncovers 54 evolutionarily conserved and
            testis-enriched genes that are not required for male fertility in
            mice
  JOURNAL   Proc Natl Acad Sci U S A 113 (28), 7704-7710 (2016)
   PUBMED   27357688
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Jiang,J., Zhang,N., Shiba,H., Li,L. and Wang,Z.
  TITLE     Spermatogenesis associated 4 promotes Sertoli cell proliferation
            modulated negatively by regulatory factor X1
  JOURNAL   PLoS One 8 (10), e75933 (2013)
   PUBMED   24146794
  REMARK    GeneRIF: RFX1 may have negative effect on cell proliferation of
            Sertoli cells via modulating Spata4 expression levels by binding
            the conserved 14-bp cis-elements of Spata4 promoter.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1057)
  AUTHORS   Duarte,D.T., Hul,S. and Sacher,M.
  TITLE     A yeast two hybrid screen identifies SPATA4 as a TRAPP interactor
  JOURNAL   FEBS Lett 585 (17), 2676-2681 (2011)
   PUBMED   21827752
REFERENCE   4  (bases 1 to 1057)
  AUTHORS   Wang,X., Harimoto,K., Liu,J., Guo,J., Hinshaw,S., Chang,Z. and
            Wang,Z.
  TITLE     Spata4 promotes osteoblast differentiation through Erk-activated
            Runx2 pathway
  JOURNAL   J Bone Miner Res 26 (8), 1964-1973 (2011)
   PUBMED   21445983
  REMARK    GeneRIF: Spata4 promotes osteoblast differentiation and
            mineralization through the Erk-activated Runx2 pathway.
REFERENCE   5  (bases 1 to 1057)
  AUTHORS   Choi,E., Lee,J., Oh,J., Park,I., Han,C., Yi,C., Kim,D.H., Cho,B.N.,
            Eddy,E.M. and Cho,C.
  TITLE     Integrative characterization of germ cell-specific genes from mouse
            spermatocyte UniGene library
  JOURNAL   BMC Genomics 8, 256 (2007)
   PUBMED   17662146
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1057)
  AUTHORS   Liu,S.F., Ai,C., Ge,Z.Q., Liu,H.L., Liu,B.W., He,S. and Wang,Z.
  TITLE     Molecular cloning and bioinformatic analysis of SPATA4 gene
  JOURNAL   J Biochem Mol Biol 38 (6), 739-747 (2005)
   PUBMED   16336790
  REMARK    GeneRIF: analysis of SPATA4 expression and variation in promotor
            sequences
REFERENCE   7  (bases 1 to 1057)
  AUTHORS   Liu,S.F., Li,L.Y., Mo,Y.Q., Fu,J.J., Liu,G., Xing,X.W. and Lu,G.X.
  TITLE     [Molecular cloning and expression in cryptorchid testis of SRG2
            from a mouse testis spermatocyte apoptosis-related gene]
  JOURNAL   Yi Chuan Xue Bao 30 (10), 943-948 (2003)
   PUBMED   14669511
  REMARK    GeneRIF: Srg2 gene was found up-regulated distinctly and playing an
            important role in cryptorchid testis.
REFERENCE   8  (bases 1 to 1057)
  AUTHORS   Liu,S.F., Li,L.Y., Fu,J.J., Liu,G., Xing,X.W. and Lu,G.X.
  TITLE     [Molecular cloning of SRG2, a mouse testis spermatocyte
            apoptosis-related gene]
  JOURNAL   Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34 (6),
            796-799 (2002)
   PUBMED   12417927
  REMARK    GeneRIF: RT-PCR showed that SRG2 was expressed significantly in
            testis.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102933.6.
            
            On Aug 22, 2018 this sequence version replaced NM_133711.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF395083.2, BC048650.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-235               AC102933.6         86208-86442
            236-365             AC102933.6         87229-87358
            366-484             AC102933.6         87739-87857
            485-705             AC102933.6         87967-88187
            706-822             AC102933.6         91392-91508
            823-1057            AC102933.6         95291-95525
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 29.27 cM"
     gene            1..1057
                     /gene="Spata4"
                     /gene_synonym="1700001N01Rik; Srg2; TSARG2"
                     /note="spermatogenesis associated 4"
                     /db_xref="GeneID:69281"
                     /db_xref="MGI:MGI:1916531"
     exon            1..235
                     /gene="Spata4"
                     /gene_synonym="1700001N01Rik; Srg2; TSARG2"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..908
                     /gene="Spata4"
                     /gene_synonym="1700001N01Rik; Srg2; TSARG2"
                     /note="spermatogenesis-related gene 2 protein; testis and
                     spermatogenesis cell-related protein 2; testis
                     spermatocyte apoptosis-related gene 2 protein"
                     /codon_start=1
                     /product="spermatogenesis-associated protein 4"
                     /protein_id="NP_598472.2"
                     /db_xref="CCDS:CCDS22308.1"
                     /db_xref="GeneID:69281"
                     /db_xref="MGI:MGI:1916531"
                     /translation="MAAAGQAEECLPLPAAESSKTSLPTPPAVPAGKKPKKCLVYPHP
                     PRSSRLSRSVLRWLQGLDLSFFPRNVTRDFSNGYLVAEIFCIYYPWDLRLSSFENGTS
                     LKVKLDNWAQIEKFLAKKKFKLPKELIHGTIHCKAGVPEILIQEIYTLLTHQEIRSIQ
                     DDLANFTDYIYQMRLPLVPRNTVSKSIKNNIRLSELLSNPNVLSNELKIEFLILLQML
                     QRKLSRKLNPGWFDVKPTVGEITIDRLPAHSYKRRYKSRGSKEKAAQPLSKSDNDGNA
                     RKEIHVKQSGNPCENTENL"
     exon            236..365
                     /gene="Spata4"
                     /gene_synonym="1700001N01Rik; Srg2; TSARG2"
                     /inference="alignment:Splign:2.1.0"
     exon            366..484
                     /gene="Spata4"
                     /gene_synonym="1700001N01Rik; Srg2; TSARG2"
                     /inference="alignment:Splign:2.1.0"
     exon            485..705
                     /gene="Spata4"
                     /gene_synonym="1700001N01Rik; Srg2; TSARG2"
                     /inference="alignment:Splign:2.1.0"
     exon            706..822
                     /gene="Spata4"
                     /gene_synonym="1700001N01Rik; Srg2; TSARG2"
                     /inference="alignment:Splign:2.1.0"
     exon            823..1057
                     /gene="Spata4"
                     /gene_synonym="1700001N01Rik; Srg2; TSARG2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atagaaaccc ataaagcacc atggctgccg ccggccaggc agaggagtgt ttaccactgc
       61 cggcggcaga atcatccaag acgtccttac ctacaccacc agcagttccc gcaggaaaga
      121 agccaaagaa atgtctggtg tatccacatc cgcctaggag ctcccgcctg agtcgttctg
      181 tcctgcgctg gctccagggc ctagatctca gcttcttccc taggaatgtc accagggatt
      241 tttcaaatgg ctacctagtt gcagagatat tctgtattta ttatccctgg gaccttcgat
      301 tatcatcctt tgaaaatgga acctccttaa aagtcaagtt ggataactgg gcacagatag
      361 agaagtttct agcgaaaaag aagtttaaac tacctaaaga actaatccat ggaacaattc
      421 actgtaaggc tggggtacct gaaatactga tacaggagat ttacacttta ctaacacatc
      481 aagaaattag aagtatccag gatgatctcg ctaatttcac agactatatt taccaaatgc
      541 gtttgcccct ggttcctagg aatacagtat caaaatctat taagaataac attaggttgt
      601 cggaactact aagcaatcct aatgtactca gcaatgaact caagatagaa ttcctcatcc
      661 ttttgcaaat gctgcaaaga aaattaagca gaaaactgaa tccagggtgg tttgatgtca
      721 agccaactgt gggagaaatc acaatcgacc gtcttcctgc ccatagctat aagcgcagat
      781 acaagtcaag aggttcaaag gagaaagctg cacaaccttt atcaaaatca gacaatgatg
      841 gcaatgcacg gaaagaaatc catgtgaagc aaagtggaaa tccttgtgag aacactgaaa
      901 acctgtgaca aatgtggaag agacagtgaa ggacatggag gaagtagctt agaagaagtg
      961 atatcactaa tgtctaccac agtctgagta tataatgtct caccccagtc ttggatgtat
     1021 ttaaataata aataacttaa attggtgtct taaccat
//
