LOCUS       NM_030047               3233 bp    mRNA    linear   ROD 07-OCT-2024
DEFINITION  Mus musculus spermatogenesis associated 31 (Spata31), mRNA.
ACCESSION   NM_030047 XM_989389
VERSION     NM_030047.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Tonelli Gombalova,Z., Kosuth,J., Alexovic Matiasova,A.,
            Zrubakova,J., Zezula,I., Giallongo,T., Di Giulio,A.M., Carelli,S.,
            Tomaskova,L., Daxnerova,Z. and Sevc,J.
  TITLE     Majority of cerebrospinal fluid-contacting neurons in the spinal
            cord of C57Bl/6N mice is present in ectopic position unlike in
            other studied experimental mice strains and mammalian species
  JOURNAL   J Comp Neurol 528 (15), 2523-2550 (2020)
   PUBMED   32212159
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Wu,Y.Y., Yang,Y., Xu,Y.D. and Yu,H.L.
  TITLE     Targeted disruption of the spermatid-specific gene Spata31 causes
            male infertility
  JOURNAL   Mol Reprod Dev 82 (6), 432-440 (2015)
   PUBMED   25930072
  REMARK    GeneRIF: Targeted disruption of the spermatid-specific gene Spata31
            causes male infertility.
REFERENCE   3  (bases 1 to 3233)
  AUTHORS   Zuo,Y., Gao,J., Yeung,W.S. and Lee,K.F.
  TITLE     The testis-specific VAD1.3/AEP1 interacts with beta-actin and
            syntaxin 1 and directs peri-nuclear/Golgi expression with bipartite
            nucleus localization (BNL) sequence
  JOURNAL   Biochem Biophys Res Commun 401 (2), 275-280 (2010)
   PUBMED   20850414
  REMARK    GeneRIF: VAD1.3 interacts with beta-actin and syntaxin 1 in vitro.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK133249.1 and AC159190.2.
            
            On Dec 19, 2009 this sequence version replaced NM_030047.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK133249.1, AY842249.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-614               AK133249.1         2-615
            615-3233            AC159190.2         101796-104414       c
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 34.21 cM"
     gene            1..3233
                     /gene="Spata31"
                     /gene_synonym="4930458L03Rik; Aep1; Fam75a; Spata31a;
                     Vad1.3"
                     /note="spermatogenesis associated 31"
                     /db_xref="GeneID:78124"
                     /db_xref="MGI:MGI:1925374"
     exon            1..260
                     /gene="Spata31"
                     /gene_synonym="4930458L03Rik; Aep1; Fam75a; Spata31a;
                     Vad1.3"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..3122
                     /gene="Spata31"
                     /gene_synonym="4930458L03Rik; Aep1; Fam75a; Spata31a;
                     Vad1.3"
                     /note="acrosome expressed protein 1; acrosome-specific
                     protein VAD1.3; family with sequence similarity 75, member
                     A"
                     /codon_start=1
                     /product="spermatogenesis-associated protein 31"
                     /protein_id="NP_084323.2"
                     /db_xref="CCDS:CCDS26602.1"
                     /db_xref="GeneID:78124"
                     /db_xref="MGI:MGI:1925374"
                     /translation="MESLPSLLESIYTTWLSLSSTIWAMDMILAFVCGLGLYHLLLPF
                     LESHLSSPPSNIKFTRKPQIQMTWQSQFKKKFRNHCRNDAKAWGECLKKLKEKEKDKL
                     FLEEMSPGHHLNSLGNIFNSSSAKQDSTTLSPFWNLKEKSEEQVATQKLSYPKISEDH
                     FQQKCDQLFWGLPSLHSESLVAAAWIPQTTSTLPSPFFLFNVISSVYPIQLQDKMSPM
                     LPHTHPLSYLDLQSPHLILSPLEFQTPALNLPVLLPSSLPYNSDFGTSYSQSQSKPQY
                     LPTEIKYNKKPSLAKQIENRSTLPLMVQKPQEAYDILAPNPSQDWVVSILPDNFPICR
                     ELREKLEQHIQKWLIQHQWNFPHKIQVSEKMKELQNTVIGNCQTRDKPGTLQALGEHS
                     NEGQKRKFQLEKESGKNLGPILGKISKDPIRGLEKTTAIKDLENNLKAHLGTKSGQID
                     QGLTPLSMRQSWLAVDDSFYEMENNLTSLKSSAKSMCSSEKLAFLKPETRQALEAHIV
                     RFWAKHRWSLPLKILKPVKLFQLSIESLPVVLCAQTSSTTSVHRTRSAAEVVRFLGKP
                     CLRQMIIEDSSPSPQNLLLVSSPSCKRARRRLPFGVDHEPSTALPTKPECGHISEDLT
                     YNFMNTTSQTRTIFKKEIETREVVLLPRRTSDQNLEAYKHQEKVVSEFPHNVETELAG
                     QPQIYTTTVLPPKRSRSMPLPVDTLTSHVLGDIVVADMDNSLVQQRPSTPKHLVSQKS
                     QIKMLAPTYQSEGTKRQSEIKYEDRPKLTPVTEKKANFGSQYYQTLPKIAQVLPGRPP
                     QRHLGRFLQWIHPKKTIKGHEFHPLKGNPTAAIVQNQRRQVRKIPYMDNNVTEAQELM
                     TTVGQMLEKKMMLQHQPYASKFNQHREVPPAPMSRFSHGHMPVSYLQQRRAPSYPGSC
                     SCQRCSVQNRHIRNQLPQRSVRFSKKPQNPRNPSHKKPQNPRNPSRLSRNAALNLVNS
                     QNRTIVPGNSNHHLYCPRHCALQRDVCRELGHSSVVFPNRKT"
     exon            261..333
                     /gene="Spata31"
                     /gene_synonym="4930458L03Rik; Aep1; Fam75a; Spata31a;
                     Vad1.3"
                     /inference="alignment:Splign:2.1.0"
     exon            334..3233
                     /gene="Spata31"
                     /gene_synonym="4930458L03Rik; Aep1; Fam75a; Spata31a;
                     Vad1.3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3199..3204
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spata31"
                     /gene_synonym="4930458L03Rik; Aep1; Fam75a; Spata31a;
                     Vad1.3"
                     /note="hexamer: AATACA"
     polyA_site      3222
                     /gene="Spata31"
                     /gene_synonym="4930458L03Rik; Aep1; Fam75a; Spata31a;
                     Vad1.3"
                     /note="major polyA site"
ORIGIN      
        1 ggagagtctc cagctctgtg ttgtgggcct gagttatcct ttagctccaa ggaagaactc
       61 ccaacaatgg gattcagatg gagagccttc cctctcttct ggaaagcatt tacaccacat
      121 ggctgagcct cagctccact atatgggcaa tggacatgat ccttgccttt gtgtgtggac
      181 tggggctcta ccacctgtta cttccttttc ttgagagtca tctgtcttca cctccatcaa
      241 atataaagtt caccaggaag cctcagatac agatgacctg gcagagccag ttcaagaaaa
      301 agtttaggaa tcattgtagg aatgatgcaa aagcttgggg agaatgcctg aaaaagctga
      361 aagaaaaaga aaaggataaa ttatttctag aagaaatgag cccaggacac catttgaact
      421 ctttagggaa tatatttaac tcatcaagtg ctaaacaaga cagcactacc ctgtcaccct
      481 tttggaacct gaaagaaaaa tcagaagagc aggtggccac tcagaaacta tcttatccca
      541 agatctcaga agaccatttt caacagaaat gtgaccagct tttctggggt ctcccttctc
      601 tccacagtga gtctctggta gctgccgcct ggatccctca gacaacgtca actctccctt
      661 ctcccttttt cttattcaat gtcatctcaa gtgtttatcc aattcaactg caggataaaa
      721 tgtcaccaat gcttccccat acacatcctc tgtcctatct ggacctccaa tctccacact
      781 taattctgtc tccacttgaa ttccagaccc cagctttgaa tctcccagtc ctactaccct
      841 cttctctacc ctataatagt gattttggta catcttattc tcaatcacaa agtaagccac
      901 aatacctccc aactgaaata aaatacaata aaaaaccctc actggcaaaa caaatagaaa
      961 atagatcaac tttacccttg atggtccaga aacctcaaga agcctatgac atcttggccc
     1021 ccaacccttc ccaagactgg gtagtctcca tccttcctga taattttccc atctgccgtg
     1081 agctccggga gaaactggag caacacattc aaaagtggct cattcaacac caatggaatt
     1141 ttccccataa gatccaagtg tctgagaaaa tgaaggagct tcagaacaca gtaataggaa
     1201 attgtcaaac cagagacaag cctggcacct tacaagccct tggtgaacac agcaatgagg
     1261 gccagaagag aaaattccag ctagaaaagg aatctggcaa gaatctggga cctattctag
     1321 gaaagatctc aaaagatcca atcaggggct tggaaaaaac tacagcaatc aaagatttag
     1381 aaaacaactt gaaagctcat ttgggtacaa agtcagggca gatagatcaa ggtctgaccc
     1441 ctctaagtat gcgacaatcc tggcttgctg tggatgatag tttttacgag atggaaaaca
     1501 atttaacatc cttaaagagt tcagcaaagt ccatgtgctc ttcagagaag cttgcctttc
     1561 tcaaaccaga aactcgacag gctctggagg cacatattgt aaggttttgg gcaaagcaca
     1621 ggtggagtct accccttaag atactcaagc ccgtaaaact ctttcagtta agtattgagt
     1681 ccttgcctgt tgtactctgt gcccagacct cctcaaccac ttctgtacat aggaccagat
     1741 cagcagctga agtagtcaga ttcctaggaa aaccatgctt gagacagatg ataattgagg
     1801 attcttctcc atcaccacag aatcttctcc ttgtctcatc tccttcctgt aagagggcca
     1861 ggagaaggct tccctttggt gttgaccatg agccttctac agccctgcca accaaaccag
     1921 agtgtgggca tatttcagaa gatctcactt ataatttcat gaacacaacc tctcagacta
     1981 ggactatctt caagaaagag atagagactc gagaagtcgt tttactgcct agaaggacga
     2041 gtgaccaaaa tttggaggca tacaaacatc aggaaaaagt tgttagtgag tttccacata
     2101 atgtggagac agaattagca ggccagccac aaatctatac cactactgtg ctccctccaa
     2161 aacgttccag gagcatgcct cttcctgtag acactttgac ttcacatgta ttaggtgaca
     2221 ttgtggtggc agacatggac aatagcctgg tgcagcagag acccagtact ccaaagcatc
     2281 tagtctcaca gaagagccaa atcaagatgt tggcccctac ttatcaaagt gagggcacta
     2341 agagacagag tgaaataaag tatgaagaca gacctaagct caccccagtc acagagaaaa
     2401 aagccaactt tggcagccaa tactaccaga cccttccaaa aatcgcacaa gtgcttccag
     2461 gaaggccccc ccaaagacat ttaggtcgct ttctacagtg gattcatcct aagaaaacaa
     2521 tcaaagggca tgaatttcat cccctaaaag gtaatcctac agcagccatt gtccagaacc
     2581 aacgaaggca agtgagaaaa ataccatata tggacaacaa tgtgactgag gcccaggaac
     2641 ttatgacaac tgttggacag atgttagaaa agaaaatgat gctgcaacat caaccctatg
     2701 cttcaaaatt caaccagcac agggaagttc ctccagcccc catgtctcgg ttttcccatg
     2761 gccacatgcc agtctcctac ttacagcaaa ggagagcacc cagttaccct ggcagttgct
     2821 catgtcagag gtgttctgta cagaacaggc acatcagaaa ccaactgcct cagagaagtg
     2881 tacgctttag caagaaacca cagaacccac ggaaccccag ccacaagaaa ccacagaacc
     2941 cacggaaccc cagccgcttg tcccgtaacg cagctttgaa cctagtgaat tctcagaaca
     3001 gaacaatagt gccaggaaat tcaaatcacc atctttattg tcctaggcac tgtgccctcc
     3061 agagagatgt ctgtagagaa ctaggacatt cctctgtagt ttttcctaat aggaaaacat
     3121 aattgaaaga aaaagattag taaatgcaaa aattattttt ctcatgttag tacattcaag
     3181 atacttaatg tagcccaaaa tacatgtttt tcccccataa aagggtaatg gtt
//
