LOCUS       NM_001122732            1104 bp    mRNA    linear   ROD 26-JAN-2025
DEFINITION  Mus musculus spermatogenesis associated 3 (Spata3), transcript
            variant 4, mRNA.
ACCESSION   NM_001122732
VERSION     NM_001122732.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Girault,M.S., Dupuis,S., Ialy-Radio,C., Stouvenel,L., Viollet,C.,
            Pierre,R., Favier,M., Ziyyat,A. and Barbaux,S.
  TITLE     Deletion of the Spata3 Gene Induces Sperm Alterations and In Vitro
            Hypofertility in Mice
  JOURNAL   Int J Mol Sci 22 (4), 1959 (2021)
   PUBMED   33669425
  REMARK    GeneRIF: Deletion of the Spata3 Gene Induces Sperm Alterations and
            In Vitro Hypofertility in Mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1104)
  AUTHORS   Wang,Y., Fang,R., Yuan,Y., Pan,M., Hu,M., Zhou,Y., Shen,B. and
            Zhao,J.
  TITLE     Identification of host proteins, Spata3 and Dkk2, interacting with
            Toxoplasma gondii micronemal protein MIC3
  JOURNAL   Parasitol Res 115 (7), 2825-2835 (2016)
   PUBMED   27053129
  REMARK    GeneRIF: Spermatogenesis-associated protein 3 (Spata3) and
            dickkopf-related protein 2 (Dkk2) were confirmed to interact with
            MIC3. The tandem repeat EGF domains of MIC3 were critical in
            mediating the interactions with the identified host proteins. The
            results show that MIC3 interacts with host proteins that are
            involved in reproduction, growth, and development.
REFERENCE   4  (bases 1 to 1104)
  AUTHORS   Li,L., Liu,G., Fu,J.J., Li,L.Y., Tan,X.J., Yang,S. and Lu,G.X.
  TITLE     Molecular cloning and characterization of a novel transcript
            variant of Mtsarg1 gene
  JOURNAL   Mol Biol Rep 36 (5), 1023-1032 (2009)
   PUBMED   18551385
  REMARK    GeneRIF: Mtsarg1 and Mtsarg1-beta may play an important role in
            mouse testicular function and in spermatocyte development.
REFERENCE   5  (bases 1 to 1104)
  AUTHORS   Fu,J.J., Lu,G.X., Li,L.Y., Liu,G., Xing,X.W. and Liu,S.F.
  TITLE     [Molecular cloning for testis spermatogenesis cell apoptosis
            related gene TSARG1 and Mtsarg1 and expression analysis for Mtsarg1
            gene]
  JOURNAL   Yi Chuan Xue Bao 30 (1), 25-29 (2003)
   PUBMED   12812072
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC102506.9.
            
            On Mar 31, 2020 this sequence version replaced NM_001122732.1.
            
            Transcript Variant: This variant (4) uses a different splice site
            in the CDS, compared to variant 2. The resulting isoform (3) lacks
            one amino acid when it is compared to isoform 2. Variants 3 and 4
            both encode the same isoform (3).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF399971.1, SRR5189685.927.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-405               AC102506.9         101104-101508
            406-577             AC102506.9         103489-103660
            578-721             AC102506.9         105573-105716
            722-1104            AC102506.9         108738-109120
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 43.94 cM"
     gene            1..1104
                     /gene="Spata3"
                     /gene_synonym="1700011N12Rik; 1700029H01Rik;
                     4930424D10Rik; Mtsarg1; Tsarg1"
                     /note="spermatogenesis associated 3"
                     /db_xref="GeneID:70060"
                     /db_xref="MGI:MGI:1917310"
     exon            1..405
                     /gene="Spata3"
                     /gene_synonym="1700011N12Rik; 1700029H01Rik;
                     4930424D10Rik; Mtsarg1; Tsarg1"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..683
                     /gene="Spata3"
                     /gene_synonym="1700011N12Rik; 1700029H01Rik;
                     4930424D10Rik; Mtsarg1; Tsarg1"
                     /note="isoform 3 is encoded by transcript variant 4;
                     testis and spermatogenesis cell apoptosis related protein
                     1; spermatogenesis-associated protein 3; testis and
                     spermatogenesis cell apoptosis related gene 1; testis
                     spermatocyte apoptosis-related protein 1; testis and
                     spermatogenesis cell-related protein 1"
                     /codon_start=1
                     /product="spermatogenesis-associated protein 3 isoform 3"
                     /protein_id="NP_001116204.1"
                     /db_xref="CCDS:CCDS15116.1"
                     /db_xref="GeneID:70060"
                     /db_xref="MGI:MGI:1917310"
                     /translation="MKKVKKKKSDSRRRRNSISPQTSSDSSQQPSSETPPSCPEPASP
                     PSKPQPCQESTTPHQVNPEPKPQQHTPQPLPPPEKPASSPFLVPMEPKPILPSRKAVP
                     LTYVAPRSCSCAACPGSSACWHRLGLCHSRIFDVLLPRDWSSMPGRGVPNLLTFYRKP
                     SRKYCAPRNSRASSSRNCCCGSGGLGSCLLHG"
     exon            406..577
                     /gene="Spata3"
                     /gene_synonym="1700011N12Rik; 1700029H01Rik;
                     4930424D10Rik; Mtsarg1; Tsarg1"
                     /inference="alignment:Splign:2.1.0"
     exon            578..721
                     /gene="Spata3"
                     /gene_synonym="1700011N12Rik; 1700029H01Rik;
                     4930424D10Rik; Mtsarg1; Tsarg1"
                     /inference="alignment:Splign:2.1.0"
     exon            722..1104
                     /gene="Spata3"
                     /gene_synonym="1700011N12Rik; 1700029H01Rik;
                     4930424D10Rik; Mtsarg1; Tsarg1"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1104
                     /gene="Spata3"
                     /gene_synonym="1700011N12Rik; 1700029H01Rik;
                     4930424D10Rik; Mtsarg1; Tsarg1"
                     /note="major polyA site"
ORIGIN      
        1 caaagatcag agacataccc acaggggaga ctgcctacct agggagggag ccaggaaatt
       61 cacagctcca tgtagctcca cggtgttact ctagaaggat caccatgaag aaggtcaaaa
      121 agaaaaagtc tgattccagg cgccgcagga attccatttc accgcagact agctccgatt
      181 cctcacagca gcccagctca gaaaccccgc cgtcatgtcc agagcccgcc tcgccaccat
      241 ctaagccaca gccttgccag gagtccacca caccacacca agttaaccct gagcctaaac
      301 cgcaacagca tacaccacag cctctcccgc ctccagagaa acctgcctca tctccgttcc
      361 tggtacccat ggaacctaaa ccaattcttc caagcaggaa agcagtgcct ctgacctatg
      421 tggccccacg ctcctgctcc tgtgcagcct gcccgggcag ctccgcttgc tggcatcgtc
      481 tggggctttg ccatagccga atctttgatg tcctcctacc tcgggactgg tcatccatgc
      541 cagggagagg agtcccaaac cttctcacct tctacagaaa accgtcaagg aagtactgcg
      601 ctccccgtaa ctctcgtgct tcaagctctc ggaactgttg ctgtggctct gggggccttg
      661 ggagttgcct acttcatggc tgaatccttg tgaattggac cccaggccca ccgtccggca
      721 ggggttcatg gcatggcacc ccaactatgc cactctgcgt aaggattatt ctaagaagaa
      781 agtcattgag aaactgaagc tcgtctctgc cctctctgat ctacctgaga acagcataat
      841 ctctgctatt acaatgaggt gacgccatcc cgggaagggt gaacagtctt gtccctgaag
      901 acagcacaca ggaaaaccat cccacacaaa gtctgccttg tctccatatc ggtgtccttc
      961 cacagtcagc caccattcac attcaaacct cttatagctt ccctacaaaa ttcttgctgt
     1021 ttatggggat gtctgtacat cttttgtgtc atttgctctg tgcacaggca ctgcctcatt
     1081 aataacttgt ttttctcttg ctaa
//
