LOCUS       NM_144827               2606 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Mus musculus spermatogenesis associated 20 (Spata20), mRNA.
ACCESSION   NM_144827 XM_354637
VERSION     NM_144827.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Wu,C., Xiao,Y., Wu,C., Xie,D., Luo,M., Yao,D., Chen,M. and Lu,D.
  TITLE     Regulation of BCRP expression and sulfasalazine pharmacokinetics by
            the nuclear receptor REV-ERBalpha
  JOURNAL   Xenobiotica 53 (3), 215-222 (2023)
   PUBMED   37039301
  REMARK    GeneRIF: Regulation of BCRP expression and sulfasalazine
            pharmacokinetics by the nuclear receptor REV-ERBalpha.
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Masola,V., Greco,N., Tozzo,P., Caenazzo,L. and Onisto,M.
  TITLE     The role of SPATA2 in TNF signaling, cancer, and spermatogenesis
  JOURNAL   Cell Death Dis 13 (11), 977 (2022)
   PUBMED   36402749
  REMARK    GeneRIF: The role of SPATA2 in TNF signaling, cancer, and
            spermatogenesis.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2606)
  AUTHORS   Liu,M., Ru,Y., Gu,Y., Tang,J., Zhang,T., Wu,J., Yu,F., Yuan,Y.,
            Xu,C., Wang,J. and Shi,H.
  TITLE     Disruption of Ssp411 causes impaired sperm head formation and male
            sterility in mice
  JOURNAL   Biochim Biophys Acta Gen Subj 1862 (3), 660-668 (2018)
   PUBMED   29247744
  REMARK    GeneRIF: Ssp411 mRNA and protein were detected exclusively in
            spermatids after step 9 during spermiogenesis in testis. Males are
            sterile in an Ssp411 expression-disrupted murine strain
            (Ssp411(PB/PB)). Ssp411 participates in the ubiquitin-proteasome
            system by interacting with PSMC3.  Ssp411 is required for
            spermatogenesis and plays a role in sperm head shaping.
REFERENCE   4  (bases 1 to 2606)
  AUTHORS   Lo,J.C., Jamsai,D., O'Connor,A.E., Borg,C., Clark,B.J.,
            Whisstock,J.C., Field,M.C., Adams,V., Ishikawa,T., Aitken,R.J.,
            Whittle,B., Goodnow,C.C., Ormandy,C.J. and O'Bryan,M.K.
  TITLE     RAB-like 2 has an essential role in male fertility, sperm
            intra-flagellar transport, and tail assembly
  JOURNAL   PLoS Genet 8 (10), e1002969 (2012)
   PUBMED   23055941
REFERENCE   5  (bases 1 to 2606)
  AUTHORS   Ding,S., Wu,X., Li,G., Han,M., Zhuang,Y. and Xu,T.
  TITLE     Efficient transposition of the piggyBac (PB) transposon in
            mammalian cells and mice
  JOURNAL   Cell 122 (3), 473-483 (2005)
   PUBMED   16096065
REFERENCE   6  (bases 1 to 2606)
  AUTHORS   Shi,H.J., Wu,A.Z., Santos,M., Feng,Z.M., Huang,L., Chen,Y.M.,
            Zhu,K. and Chen,C.L.
  TITLE     Cloning and characterization of rat spermatid protein SSP411: a
            thioredoxin-like protein
  JOURNAL   J Androl 25 (4), 479-493 (2004)
   PUBMED   15223837
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC050788.1.
            
            On Jun 18, 2008 this sequence version replaced NM_144827.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050788.1, SRR5189685.169630.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2606              BC050788.1         1-2606
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 58.9 cM"
     gene            1..2606
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /note="spermatogenesis associated 20"
                     /db_xref="GeneID:217116"
                     /db_xref="MGI:MGI:2183449"
     exon            1..101
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..2397
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /note="sperm protein SSP411; ssp411; sperm-specific
                     protein 411; transcript increased in spermiogenesis 78
                     protein"
                     /codon_start=1
                     /product="spermatogenesis-associated protein 20"
                     /protein_id="NP_659076.2"
                     /db_xref="CCDS:CCDS25256.1"
                     /db_xref="GeneID:217116"
                     /db_xref="MGI:MGI:2183449"
                     /translation="MSHHSSPPPKHKGEHKGHGLPRGSERGSSSRDKDRSATTVSSSA
                     PMPAGGKSSRTNCPPPAPPKTVNRLINEKSPYLLQHAYNPVDWYPWGQEAFDKAKKEN
                     KPIFLSVGYSTCHWCHMMEEESFQNEEIGRLLNENFICVMVDREERPDVDKVYMTFVQ
                     ATSSGGGWPMNVWLTPGLQPFVGGTYFPPEDGLTRVGFRTVLMRICDQWKLNKNTLLE
                     NSQRVTTALLARSEISVGDRQIPASAATMNSRCFQQLDEGYDEEYGGFAEAPKFPTPV
                     ILNFLFSYWLSHRLTQDGSRAQQMALHTLKMMANGGIQDHVGQGFHRYSTDRQWHIPH
                     FEKMLYDQAQLSVVYTQAFQISGDEFYADVAKGILQYVTRTLSHRSGGFYSAEDADSP
                     PERGMKPQEGAYYVWTVKEVQQLLPEPVVGASEPLTSGQLLMKHYGLSEVGNINSSQD
                     PNGELHGQNVLMVRYSLELTAARYGLEVEAVRALLNTGLEKLFQARKHRPKAHLDNKM
                     LAAWNGLMVSGFAVTGAALGMEKLVAQATSGAKFLKRHMFDVSSGRLKRTCYAGTGGT
                     VEQSNPPCWGFLEDYAFVVRGLLDLYEASQESSWLEWALRLQDTQDKLFWDPRGGGYF
                     CSEAELGADLPLRLKDDQDGAEPSANSVSAHNLLRLHSFTGHKDWMDKCVCLLTAFSE
                     RMRRVPVALPEMVRTLSAQQQTLKQIVICGDPQAKDTKALLQCVHSIYVPNKVLILAD
                     GDPSSFLSRQLPFLSSLRRVEDRATVYIFENQACSMPITDPCELRKLLHQ"
     exon            102..284
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            285..349
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            350..504
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            505..648
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            649..850
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            851..981
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1086
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1161
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1371
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1564
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1733
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1945
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            1946..2145
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            2146..2226
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..2589
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2563..2568
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /note="hexamer: AATAAA"
     polyA_site      2589
                     /gene="Spata20"
                     /gene_synonym="Tisp78"
                     /note="major polyA site"
ORIGIN      
        1 gactaacctg gcagggagcc cccaatgagc caccattcct caccaccccc aaaacataag
       61 ggggagcaca aaggccacgg tctcccccgt ggttcagaaa ggggcagctc ttcccgggac
      121 aaggaccgaa gtgctactac agttagtagt tcagcgccca tgcctgctgg aggaaagtca
      181 agccggacta actgtcctcc acctgcaccg ccgaagaccg tcaatcgcct gatcaatgag
      241 aagtctccat acctcttaca gcatgcctac aacccggtgg attggtaccc ctggggacag
      301 gaagccttcg acaaggccaa aaaagaaaac aagccgattt ttctctcagt ggggtactct
      361 acctgtcact ggtgccacat gatggaggag gaatccttcc agaatgaaga gatagggcgc
      421 ctgctcaatg agaacttcat ctgtgtcatg gtggaccgag aggagcggcc agatgtggac
      481 aaagtataca tgacttttgt gcaggccacc agtagtggag gaggctggcc aatgaatgtg
      541 tggctgaccc ccggcctcca gccattcgtt gggggcacct acttcccgcc agaggatggt
      601 ttgactcgag ttggcttccg aacagtgttg atgagaatct gtgatcagtg gaaactgaac
      661 aagaacaccc ttctggagaa cagccagcgc gtcaccacag cccttctggc ccgatctgag
      721 atcagcgtgg gtgacagaca gattccagcc tctgctgcca ccatgaacag ccgttgcttc
      781 cagcagctgg atgagggcta tgatgaggaa tacggcggct tcgccgaggc ccccaagttt
      841 cccacacctg tgatcctgaa cttcctgttc tcctattggc tcagtcaccg gctcacccag
      901 gatggctccc gagctcagca gatggcgttg cacaccctga agatgatggc caacggaggc
      961 atccaggatc atgtggggca gggctttcac cgctactcca cggaccgaca gtggcacatc
     1021 cctcactttg agaaaatgct gtatgaccag gcccaactct cggtggtcta tacccaggcc
     1081 ttccagatct ctggcgatga attctacgct gatgttgcta aaggcatcct gcagtatgtg
     1141 acccggactc tgagccatcg gtctggaggc ttttacagtg cagaggatgc tgactccccc
     1201 ccggagagag gcatgaagcc ccaagagggt gcttactatg tgtggacagt caaagaggtc
     1261 caacagctac tcccagagcc cgtggtaggc gccagcgagc cgctgacctc aggccagctc
     1321 ctaatgaagc actatggact cagtgaagta ggcaatatca actcctctca ggatcccaac
     1381 ggggaactgc acggccagaa tgtcctgatg gtgcgttact cactagagct gactgcagcc
     1441 cgctatggcc tggaggtgga ggctgtgagg gccttgctca acacagggct ggagaagctc
     1501 ttccaggccc ggaaacatcg gccgaaagca cacctagaca acaagatgct cgctgcctgg
     1561 aacggcttga tggtttctgg ctttgctgtg acgggggctg ccctgggtat ggaaaagtta
     1621 gttgcccagg ccaccagcgg tgccaaattc cttaagcggc acatgtttga tgtgtccagc
     1681 ggccgcctga agaggacatg ctatgctggc accggaggaa ctgtggagca aagcaaccca
     1741 ccatgctggg gtttcctgga ggactatgcc tttgtggtac ggggcctcct ggacctgtac
     1801 gaggcctcac aggagagctc atggcttgag tgggctctgc gcctgcagga cacacaggac
     1861 aagctcttct gggacccccg aggtggtggc tacttctgca gtgaggctga gctgggggca
     1921 gacctgcctc ttcgtctgaa ggatgaccag gatggcgcag agcccagtgc taactctgtg
     1981 tcggcccaca acctccttcg gttgcacagc ttcactggcc acaaggattg gatggacaag
     2041 tgtgtgtgcc tgctgacagc cttctctgag cgcatgcgcc gcgtcccagt ggccttgcct
     2101 gagatggtcc gtaccctctc agctcaacag cagactctga agcagattgt gatctgtgga
     2161 gacccccagg ccaaggacac caaggccctg ctgcaatgtg tccactccat ctacgtccct
     2221 aacaaggtgc tcattctggc tgatggagac ccatcgagct tcctgtcccg tcagcttccc
     2281 ttcttgagca gccttcgaag agtagaagac cgggccacag tctacatatt tgagaaccaa
     2341 gcctgctcca tgcctatcac ggatccctgt gagttacgaa agctgctaca ccaatgactg
     2401 cccagaaccc tatgagctgg gccagaaggc agaattttcc aactgaccag agactcaggc
     2461 cttgagaggc taataccaaa cctatagcta ctcctgggta ccctgcctcc aggtgatcac
     2521 agccatcctt gcggctcccc catgggcacc tactcaccat gaaataaacc taacagtgtc
     2581 ccgtggtgaa aaaaaaaaaa aaaaaa
//
