LOCUS       NM_001411395            4260 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus spermatogenesis associated 2 (Spata2), transcript
            variant 5, mRNA.
ACCESSION   NM_001411395 XM_017318649
VERSION     NM_001411395.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Griewahn,L., Muller-Foxworth,M., Peintner,L., Wissler,M., Weiss,M.,
            Brauns-Schubert,P., Massoumi,R., Borner,C., Gross,O., Yabal,M.,
            Charvet,C. and Maurer,U.
  TITLE     SPATA2 restricts OTULIN-dependent LUBAC activity independently of
            CYLD
  JOURNAL   Cell Rep 42 (1), 111961 (2023)
   PUBMED   36640323
  REMARK    GeneRIF: SPATA2 restricts OTULIN-dependent LUBAC activity
            independently of CYLD.
REFERENCE   3  (bases 1 to 4260)
  AUTHORS   Yang,X.D., Li,W., Zhang,S., Wu,D., Jiang,X., Tan,R., Niu,X.,
            Wang,Q., Wu,X., Liu,Z., Chen,L.F., Qin,J. and Su,B.
  TITLE     PLK4 deubiquitination by Spata2-CYLD suppresses NEK7-mediated NLRP3
            inflammasome activation at the centrosome
  JOURNAL   EMBO J 39 (2), e102201 (2020)
   PUBMED   31762063
REFERENCE   4  (bases 1 to 4260)
  AUTHORS   Zhao,J., Zhao,J., Xu,G., Wang,Z., Gao,J., Cui,S. and Liu,J.
  TITLE     Deletion of Spata2 by CRISPR/Cas9n causes increased inhibin alpha
            expression and attenuated fertility in male mice
  JOURNAL   Biol Reprod 97 (3), 497-513 (2017)
   PUBMED   29025062
  REMARK    GeneRIF: Our data revealed the impact of SPATA2 on male fertility
            and suggested that SPATA2 ensures the normal secretory function of
            Sertoli cells
REFERENCE   5  (bases 1 to 4260)
  AUTHORS   Wei,R., Xu,L.W., Liu,J., Li,Y., Zhang,P., Shan,B., Lu,X., Qian,L.,
            Wu,Z., Dong,K., Zhu,H., Pan,L., Yuan,J. and Pan,H.
  TITLE     SPATA2 regulates the activation of RIPK1 by modulating linear
            ubiquitination
  JOURNAL   Genes Dev 31 (11), 1162-1176 (2017)
   PUBMED   28701375
  REMARK    GeneRIF: Spata2 can regulate inflammatory response and cell death
            in both RIPK1-dependent and RIPK1-independent manners
            Erratum:[Genes Dev. 2018 Dec 1;32(23-24):1591. doi:
            10.1101/gad.321075.118. PMID: 30509949]
REFERENCE   6  (bases 1 to 4260)
  AUTHORS   Schlicher,L., Wissler,M., Preiss,F., Brauns-Schubert,P., Jakob,C.,
            Dumit,V., Borner,C., Dengjel,J. and Maurer,U.
  TITLE     SPATA2 promotes CYLD activity and regulates TNF-induced NF-kappaB
            signaling and cell death
  JOURNAL   EMBO Rep 17 (10), 1485-1497 (2016)
   PUBMED   27458237
  REMARK    GeneRIF: this study identifies SPATA2 as an important factor in the
            TNF signaling pathway with a substantial role for the effects
            mediated by the cytokine.
REFERENCE   7  (bases 1 to 4260)
  AUTHORS   Kupka,S., De Miguel,D., Draber,P., Martino,L., Surinova,S.,
            Rittinger,K. and Walczak,H.
  TITLE     SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to
            Signaling Complexes
  JOURNAL   Cell Rep 16 (9), 2271-2280 (2016)
   PUBMED   27545878
  REMARK    GeneRIF: SPATA2 has been described as a previously unrecognized
            factor in the linear ubiquitin chain assembly complex-dependent
            signaling pathways that serves as an adaptor between HOIP and CYLD,
            thereby enabling recruitment of CYLD to signaling complexes.
REFERENCE   8  (bases 1 to 4260)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL589870.30.
            
            On Aug 25, 2022 this sequence version replaced XM_017318649.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13862062.75384.1,
                                           SRR14995048.2251162.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164136, SAMN01164140
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-116               AL589870.30        48810-48925         c
            117-316             AL589870.30        45220-45419         c
            317-377             AL589870.30        42483-42543         c
            378-813             AL589870.30        41209-41644         c
            814-4260            AL589870.30        37184-40630         c
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 87.23 cM"
     gene            1..4260
                     /gene="Spata2"
                     /gene_synonym="mKIAA0757"
                     /note="spermatogenesis associated 2"
                     /db_xref="GeneID:263876"
                     /db_xref="MGI:MGI:2146885"
     exon            1..116
                     /gene="Spata2"
                     /gene_synonym="mKIAA0757"
                     /inference="alignment:Splign:2.1.0"
     exon            117..316
                     /gene="Spata2"
                     /gene_synonym="mKIAA0757"
                     /inference="alignment:Splign:2.1.0"
     exon            317..377
                     /gene="Spata2"
                     /gene_synonym="mKIAA0757"
                     /inference="alignment:Splign:2.1.0"
     exon            378..813
                     /gene="Spata2"
                     /gene_synonym="mKIAA0757"
                     /inference="alignment:Splign:2.1.0"
     exon            814..4260
                     /gene="Spata2"
                     /gene_synonym="mKIAA0757"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3971..3976
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spata2"
                     /gene_synonym="mKIAA0757"
                     /note="hexamer: AATAAA"
     polyA_site      3998
                     /gene="Spata2"
                     /gene_synonym="mKIAA0757"
                     /note="major polyA site"
     regulatory      4239..4244
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spata2"
                     /gene_synonym="mKIAA0757"
                     /note="hexamer: ATTAAA"
     polyA_site      4260
                     /gene="Spata2"
                     /gene_synonym="mKIAA0757"
ORIGIN      
        1 acttccgggc tgcttttgcc cccggtactg ttctcggttc ccggagccgg cgggagcggc
       61 cgggccgtgt gggcctgtgc tttggaggcg ctcgctttcc tggcaggcag cggtgggtct
      121 tcagcagaag gtgtggccca gattaaaggt gggacaccac gcctggatct gggacttgtt
      181 tttgtcccag gctggccttg aactcagatc tctttgcttc agtctcctgg gattcatagc
      241 cactgtgcct caagatctcc atgccaagat ccaggtcaga aacttgtatc tcccagcttc
      301 aagacctgga tcgcaggtcc cctgtgagct gctgggctgt gtgcttcttc tcgcagctgg
      361 ctgcggcccc ctctaagagc atgcgctggt ccatgggcac caggccagtt cttcatgtga
      421 tgtgccagcc tccagttcca gagccatggg ggagatgagg tagcccttgg gtttgatttg
      481 gggaagccca gttcaatgga tacgaagtac aaggatgact tattccggaa gtacgtgcag
      541 ttccatgagg gcaaagtgga caccaccccc ggcaaccagc agcctggcag cgatgagtac
      601 ctgcgcgtgg cagctgccac cctgctcagc ctgcacaagg tggacccttt atatcgattt
      661 cggctgatcc agttttatga ggtggtggag agctccctcc gctcgctgag cagctccagc
      721 ctgagtgctc tgcactgcgc cttcagcatg ctggagacaa tggccatcaa cctcttcctg
      781 ttcccctgga agaaggagtt ccgcagcatc aagacctaca ctggcccttt tgtttactat
      841 gtcaagtcca cgttgctgga gaaggacatc cgagccattc taaggttcat gggctacgag
      901 cctgagttgg ggactgtgta caaactcaaa gagcttgtgg agtccctcca ggtgaagatg
      961 gtctcctttg agctcttcct ggccaaggtc gagtgtgagc agatgctggg catccactcg
     1021 caggtgaagg acaagggcta ctcagagctg gacgtggtgg ctgagcgcaa gggcagcacg
     1081 gaggatgcgc gcgggtgctc agatgctctg cggaggcggg ctgagagtcg ggagcacctg
     1141 accacttcca tggctcgtgt ggcactccag aaatcagcca gtgagcgagc agccaaggac
     1201 tactacaagc cccgggtgac caaaccctcc aggtctgtgg atgcctacga cagctactgg
     1261 gagagcagga agcccccttc aaaggcctca ctgagtctgc gcaaggagcc cctggccatg
     1321 gatgtagggg aagacttgaa ggacgagatc atccgcccat ccccctcatt gctggccatg
     1381 tccagctccc cccatggtag ccctgatgac ctttcctcca tctcctccat caatggcctt
     1441 ggccttcttc gtagtacata cttttccact caggatgacg tggacctgta tacggattca
     1501 gaacccaggg ccacctaccg gaggcaggat gctctgcggc ccgatgtgtg gctggtcaaa
     1561 aatgataccc accccatcta ccacaagcgt tcacccccca ccaaagagtc tgccctctcc
     1621 aagtgccaaa actgcggcct gtcctgcagc tcctccctct gccagcgctg tgacagtgtg
     1681 ctggtctgtc cttcggcctc caagcccagc gctttcccca gcaaggcttc tgtacacgac
     1741 agcttggccc acggggcacc tatgcgggag aagtatgtgg gccaccagac tcagggcctt
     1801 gaccggctgg cacctgtcca ctcaaagccc aagccctcta ccacagccac ctcccgctgt
     1861 ggcttctgta accgtgcagg tgccaccaac acgtgcaccc agtgttcaaa agtttcctgt
     1921 gacgcctgcc tcggcgccta ccactacgac ccgtgctgca gaaagagtga gctgcacaag
     1981 ttcctgccca acagtcagct gaactacaag tccgccccgt tctcccagct cgtgtacaga
     2041 taggcctcac cccccgtctt ccgctacaag ggctacacac cactgacctg gcttggtccc
     2101 atcaagaggg aacagagctg caccggaagg aagcagagac ttgagcttga ccgtggaagc
     2161 accagggtta ctgctttgtc acatggctcc cgtagcaggt cacattccag ccagtggctg
     2221 ctgtgtggtc cgaaggacag gatggcactt ccattcagtc acttcgctgc cctctccact
     2281 tcctgctcag ctggtatggg tttgggttct ggttctggtt cccttgaaat aagtctctgt
     2341 ctctgggact cttgccacac ctgccccacc cactgcaaag ccagcttgga ctgggaagga
     2401 gcctttgggg tcaccttcaa atccccacca ggagtggtga gctagaggcc tgctggcttg
     2461 tgaaatgagc cctcctgcca caccgggcct ctctggtggc tcgcctcttt tcctgctccc
     2521 ttgatacaag gatccctagg cagatgtccc caccccttgt ctgccccctg actggtatta
     2581 atgggtagcc aagtccagta ttagtcgaat gccatcttgc cagctccact caaccagccc
     2641 acaatgttgg caagctctgc ttggcagttg gaaagtggac tccatagcag gactgacgtc
     2701 ccctcctgtt tgcataaccc ccttactgta ttgtacatac agaatttgag gtttattttt
     2761 taatgagtgt tcaacttgct gtgtgtttca acatggtgaa agaaattaat gatacggtat
     2821 ttataaaggt gccctgggtt ctttagtctt ccaagggggg ccgcatgccc tttccccgtg
     2881 tttcttccac agctagggtg gagctgggag cctggcgtaa gggactgata aagcccatgt
     2941 tccttcccat ggtggtagag agctactgtc ttccctacgg taaatattat acacctacct
     3001 ccagtgtgca cacaggggtt agcatggtgc cggggcaatg acctcagtga caagatgggg
     3061 acctcagccc ccacgtctat agctctgtct ggtcttggtg cctgctgagc atgaatcttg
     3121 taccctgaac cttgaatctc agaaggaagt tcaggcggtg ggtgcctcca cccccatcta
     3181 gccttcctca ctggttcccg gggctgtcag cgcggctctc agtctttttg cactagggcg
     3241 ggcagaggtg ggatgcgctc cttagatgcc tctccagtat tcagtgtcca cacttcctgg
     3301 gataaatccc acctctgggt tttccttttt ggggagaggg gcagggcagg aatttgaagc
     3361 tgtcacttcc ttgtgaagtg tgaacacagt ttagccatag gcagttgtga tgtccagtgt
     3421 ttgggatcta tgggcaagta gttttcccaa agcaggcatt cagttctgga gcaggcttga
     3481 ctctggccaa accctgagcc actgtgaatt tgctggtggt cttccgactt actggagtga
     3541 ttttttaggt tttccaaatc ttatatgact ctttttttat ctctagctga tagaccgcat
     3601 gtaattaggt gtacatacag cagactctgg gcagttttat tcctgagact ggccttcctg
     3661 ggcagctctt tgatgtcagc acctcagacc tggcaaggcc tggccctttc ctgagagctc
     3721 cagctctgca ttccagtcac tccagcattg cagggactct cttaagatgg aggacaggcc
     3781 acctgaggaa gtccagtgtg ttccaccagg aaatacagtg tacagactgg caaggcctct
     3841 cccctcctag gtgtgtctca ctataggaga tcgctgtgat ccgggtagtt gagtatatgt
     3901 taatttaaaa tgcacatatt ttgcatttga tttctacgga gttgaaatgt tgagagattt
     3961 aaaaaaaaaa aataaagtgt cttccattgt taaatgaagt gttcatggtg ttacagcccc
     4021 ttctgtggct tccctcattt atttgcagca tgtgctggcc tagacaccac agttagctca
     4081 agtgacagga ctcagacgga tcctactgct ttggtcatct ggggaagctg gaaaccaggc
     4141 ccgaggtcaa gctgtctgtt ttcccaaagt atttctggga aaaaataatt gttactagta
     4201 aaggcacaac ttttttcccc tgccttctga tttttctcat taaaggaata ttctaccaca
//
