LOCUS       NM_178387               1949 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus spermatogenesis associated 18 (Spata18), mRNA.
ACCESSION   NM_178387 XM_001478951 XM_001479212
VERSION     NM_178387.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ikari,N., Honjo,K., Sagami,Y., Nakamura,Y. and Arakawa,H.
  TITLE     Mieap forms membrane-less organelles involved in cardiolipin
            metabolism
  JOURNAL   iScience 27 (2), 108916 (2024)
   PUBMED   38322995
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Hamada,K., Kurashige,T., Shimamura,M., Arakawa,H., Nakamura,Y. and
            Nagayama,Y.
  TITLE     MIEAP and ATG5 are tumor suppressors in a mouse model of
            BRAFV600E-positive thyroid cancer
  JOURNAL   Front Endocrinol (Lausanne) 13, 932754 (2022)
   PUBMED   36187114
  REMARK    GeneRIF: MIEAP and ATG5 are tumor suppressors in a mouse model of
            BRAF(V600E)-positive thyroid cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1949)
  AUTHORS   Dan,X., Babbar,M., Moore,A., Wechter,N., Tian,J., Mohanty,J.G.,
            Croteau,D.L. and Bohr,V.A.
  TITLE     DNA damage invokes mitophagy through a pathway involving Spata18
  JOURNAL   Nucleic Acids Res 48 (12), 6611-6623 (2020)
   PUBMED   32453416
  REMARK    GeneRIF: DNA damage invokes mitophagy through a pathway involving
            Spata18.
REFERENCE   4  (bases 1 to 1949)
  AUTHORS   Tsuneki,M., Nakamura,Y., Kinjo,T., Nakanishi,R. and Arakawa,H.
  TITLE     Mieap suppresses murine intestinal tumor via its mitochondrial
            quality control
  JOURNAL   Sci Rep 5, 12472 (2015)
   PUBMED   26216032
  REMARK    GeneRIF: findings suggest that the Mieap-regulated mitochondrial
            quality control plays a critical role in preventing mouse
            intestinal tumorigenesis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1949)
  AUTHORS   Bornstein,C., Brosh,R., Molchadsky,A., Madar,S., Kogan-Sakin,I.,
            Goldstein,I., Chakravarti,D., Flores,E.R., Goldfinger,N., Sarig,R.
            and Rotter,V.
  TITLE     SPATA18, a spermatogenesis-associated gene, is a novel
            transcriptional target of p53 and p63
  JOURNAL   Mol Cell Biol 31 (8), 1679-1689 (2011)
   PUBMED   21300779
  REMARK    GeneRIF: show that SPATA18 transcription is induced by p53 in a
            variety of cell types of both human and mouse origin
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140429.2.
            
            On or before Apr 10, 2009 this sequence version replaced
            XM_001478951.1, XM_001479212.1, NM_178387.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.190321.1,
                                           SRR7652917.613652.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-293               AC140429.2         124471-124763
            294-399             AC140429.2         130586-130691
            400-515             AC140429.2         130834-130949
            516-622             AC140429.2         132319-132425
            623-718             AC140429.2         139943-140038
            719-925             AC140429.2         141613-141819
            926-1220            AC140429.2         142530-142824
            1221-1382           AC140429.2         144203-144364
            1383-1558           AC140429.2         145429-145604
            1559-1682           AC140429.2         148307-148430
            1683-1766           AC140429.2         150030-150113
            1767-1812           AC140429.2         152083-152128
            1813-1949           AC140429.2         152439-152575
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 39.03 cM"
     gene            1..1949
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /note="spermatogenesis associated 18"
                     /db_xref="GeneID:73472"
                     /db_xref="MGI:MGI:1920722"
     exon            1..293
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..1820
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /note="spermatogenesis-associated protein 18;
                     spermatogenesis associated 18 homolog"
                     /codon_start=1
                     /product="mitochondria-eating protein"
                     /protein_id="NP_848474.2"
                     /db_xref="CCDS:CCDS57351.1"
                     /db_xref="GeneID:73472"
                     /db_xref="MGI:MGI:1920722"
                     /translation="MAESLKKLAKSESLQALQDKVTYWVNDYNSNSCDQNLNYCIELI
                     EQVAKVQAQLFGILTVTAQEGGNNEGVETIKCRLLPLLQTSFSSVNMGKTAESEMCAT
                     QDFQLRSKNRDNSPDQDQHQSDNESFSETQPTQVQDDLAESGKSLEGAKNGSTISLLA
                     AEEEINQLKKQLKSLQAQEDARHKTSENRRSEALKSDHRSTKRTQDQRPQDVVSNYEK
                     HLQNLKEEIAVLSAEKSGLQGRSARSPSPSTGTRSHRRGRSRSHSRSRSHSRSNSPCT
                     TVAKIRSPSPNRAKMSSVARKAALLSRFSDAYSQARLDAQCLLRRCIDRAETVQRIIY
                     IATVEAFHVAKMAFRHFKIRVRKMLTPSNVGSNTDFETAVSEYIVCHLDLYDSQSSVN
                     DVIRAMNVNPKISFPPEVDFCLLTDFIQEICCIAFAMQSLEPPLDIAFGADGEIFNDC
                     KYRRSYDSDFTAPLVFYHVWPALMENDCVIMKGEAVTKRGAFWSSVRPVMRCRSRSLS
                     PICPRNHFGISTVSRSRSPSPIRCTFARY"
     exon            294..399
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     exon            400..515
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     exon            516..622
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     exon            623..718
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     exon            719..925
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1220
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1382
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1558
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     exon            1559..1682
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1766
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     exon            1767..1812
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..1949
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1918..1923
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /note="hexamer: AATGAA"
     polyA_site      1949
                     /gene="Spata18"
                     /gene_synonym="1700067I02Rik; Mieap"
                     /note="major polyA site"
ORIGIN      
        1 gcgggggagc cagaacagcg cgccgggcag gggggacctg cccggtccga cccgaggggg
       61 aggatggaca ctccagccgc gttctgagcc tgtgtggaag ataacatcct taagctacat
      121 caccccgtcg ttcagcctaa gccacccact ccctcgtccc ctctactact agatctcccg
      181 gagtcggaag cgcgaaaaag cgcgcgatgg cagaaagcct gaagaagctg gccaaaagcg
      241 aatctttaca agcactgcag gataaagtca cctactgggt gaacgactac aactcaaact
      301 cctgtgatca aaatctaaat tattgcatcg aactcatcga acaagttgct aaagtgcagg
      361 cacagctctt tgggatcctc acagtgacag cccaagaagg gggaaataat gaaggcgtgg
      421 agacaatcaa gtgtcgcctt ttgcccttgc tgcagacttc cttcagttct gtaaacatgg
      481 ggaaaacggc tgagtctgag atgtgtgcta cacaggattt ccaacttaga agtaaaaaca
      541 gagacaattc tccggatcag gaccaacatc aatcagacaa tgaatcgttc tcagagactc
      601 aaccaaccca ggttcaagac gatctggctg aaagtggaaa gagtcttgaa ggagccaaga
      661 acggatcaac catatccctc ctggctgcag aggaggagat aaaccagcta aaaaagcagc
      721 tcaaatctct ccaagctcag gaggacgccc gccacaaaac ctcggagaac cgtcgctctg
      781 aggccttaaa aagtgatcat cgatcaacca aacgaaccca ggaccaacgg ccccaagacg
      841 tggtgtccaa ctatgagaaa catctgcaaa atctgaagga agagatagct gtcctgtctg
      901 ctgagaaaag tggtctccag gggaggtctg ccaggagccc atctcctagc acaggcaccc
      961 gcagccaccg tcgcggccgc agccgtagcc acagccgcag ccgcagccac agccggtcca
     1021 atagcccctg caccacagtg gccaagatca gaagcccgtc cccaaaccgt gccaaaatgt
     1081 ccagcgtggc tcgcaaagct gctctcctgt cccgattcag cgacgcctat tcccaggccc
     1141 gtctggatgc tcagtgcttg ctgaggcgtt gcatcgacag ggcagagacg gtgcagcgga
     1201 tcatttacat cgccacagtg gaggcatttc atgtagctaa aatggcattc aggcatttca
     1261 agatccgggt gaggaagatg ctgacgccat cgaacgtggg atctaatact gactttgaga
     1321 ccgcagtctc cgaatacatt gtttgccacc tggacctgta tgactcgcaa agcagtgtca
     1381 atgatgtgat ccgtgccatg aacgtcaacc ccaagatctc attccctcct gaagttgact
     1441 tctgcctcct cactgacttc atccaggaga tttgctgcat tgcttttgca atgcagtcct
     1501 tagagccacc cctcgatatc gcattcgggg cagatgggga aatctttaac gattgcaagt
     1561 accgccgcag ctatgactcg gactttactg ctcccttggt cttttatcac gtgtggcctg
     1621 ctctcatgga gaatgactgt gtcattatga agggagaagc tgtcaccaaa agaggggctt
     1681 tttggagttc cgtgcgacca gtaatgcggt gccgtagcag gagcttaagc cccatttgtc
     1741 ctcgtaatca ttttggaata agcacggtat ctcgaagtcg aagcccttct ccaataagat
     1801 gtacattcgc aaggtactga atccaccagc cacgttcctt tgccaccgtt ggaacagcca
     1861 gcttcctccg agcacccacc cacctgcctt gtctgcctcc gacctcgctt aacatgaaat
     1921 gaaaaggcaa ttctgtgtgt cacttggca
//
