LOCUS       NM_029150               2011 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus spermatogenesis associated 16 (Spata16), transcript
            variant 2, mRNA.
ACCESSION   NM_029150
VERSION     NM_029150.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Fujihara,Y., Oji,A., Larasati,T., Kojima-Kita,K. and Ikawa,M.
  TITLE     Human Globozoospermia-Related Gene Spata16 Is Required for Sperm
            Formation Revealed by CRISPR/Cas9-Mediated Mouse Models
  JOURNAL   Int J Mol Sci 18 (10), 2208 (2017)
   PUBMED   29065458
  REMARK    GeneRIF: The point mutation in Spata16 was not essential for male
            fertility; however, deletion of the fourth exon of Spata16 resulted
            in infertile male mice due to spermiogenic arrest but not
            globozoospermia. This study demonstrates that Spata16 is
            indispensable for male fertility in mice, as well as in humans.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Xu,M., Xiao,J., Chen,J., Li,J., Yin,L., Zhu,H., Zhou,Z. and Sha,J.
  TITLE     Identification and characterization of a novel human
            testis-specific Golgi protein, NYD-SP12
  JOURNAL   Mol Hum Reprod 9 (1), 9-17 (2003)
   PUBMED   12529416
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK076628.1, BC132205.1 and AC108829.13.
            
            On Apr 6, 2010 this sequence version replaced NM_029150.3.
            
            Transcript Variant: This variant (2) represents the longer
            transcript and encodes the longer isoform (2).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK076628.1, BC132539.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-76                AK076628.1         2-77
            77-1949             BC132205.1         1-1873
            1950-2011           AC108829.13        71064-71125         c
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 10.74 cM"
     gene            1..2011
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /note="spermatogenesis associated 16"
                     /db_xref="GeneID:70862"
                     /db_xref="MGI:MGI:1918112"
     exon            1..107
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /inference="alignment:Splign:2.1.0"
     exon            108..740
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..1841
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /note="isoform 2 is encoded by transcript variant 2;
                     spermatogenesis-associated protein 16;
                     spermatogenesis-related protein"
                     /codon_start=1
                     /product="spermatogenesis-associated protein 16 isoform 2"
                     /protein_id="NP_083426.1"
                     /db_xref="CCDS:CCDS17269.1"
                     /db_xref="GeneID:70862"
                     /db_xref="MGI:MGI:1918112"
                     /translation="MDSGKSRSLETTVKRVYRDLLLPKINTSKKMSTLTNSPSSLEGT
                     PGIKKNSGEMQAEASAERVKLTKSIKEKQNNELEKVALKRKADSEEKLVGKKEAKIME
                     LDNQLVTTVPLPHIPLKNIMDVEMKLVYVDEEDVSYEFAQPNMCLGLQATGQTATTMP
                     PASPRDLTTLPQIDKWLQVALKDASSCYRQKKYAVAAGQFRTALELCSKGAALGKPFE
                     AHAEDIASIASFIETKLVTCYLRMRKPDLALNHAHRSIVLNPAYFRNHLRQAAVFRCL
                     ERYSEAARSAMIADYMFWLCGGSEHSVSKLIKLYWQAMIEEAITRAEAFSVMYTPFAT
                     RIKPENIEKVKEVFMRTHPTYVDCIYTDTQGLHVLPQTADWSCFPPQQYLLTLGFKNK
                     EDGKFLEKVSSRKLPTYTEHKTPFSPLTREDTVRHMETVGKRILPILDFIRSTQLNGN
                     FHACSGVMEKLHYASLLSRLQRVKEQAQVINQAMAELATVPYLQDISQQEAELLQSLM
                     ADAMDTLEGKKSDKERVWNTIQKVGRIEDFLYQLEDSFLKTKKLRTARRQKTKMKRLQ
                     TVQQN"
     exon            741..886
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /inference="alignment:Splign:2.1.0"
     exon            887..976
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1061
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1209
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1356
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1469
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1634
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /inference="alignment:Splign:2.1.0"
     exon            1635..1718
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..2011
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1981..1986
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /note="hexamer: AATAAA"
     polyA_site      2004
                     /gene="Spata16"
                     /gene_synonym="4921511F01Rik; 4930503K02Rik; Nyd-sp12"
                     /note="major polyA site"
ORIGIN      
        1 aagagctctc tgaagctcca cagcaggttg gcctgggatt gggaccgttg gcacgacaga
       61 tgctgtaggt ggtagtcact cggctcctct gaccctaaac cacacaggag tctacgggtg
      121 gaccaatgga ttctggcaag agtaggagtt tggagactac agtgaagaga gtctatcgag
      181 atctgcttct tccaaagata aatacaagca agaaaatgtc caccttgaca aattccccca
      241 gcagcctgga agggacacct ggaattaaga agaacagtgg agaaatgcaa gctgaagcct
      301 cagctgagag agtaaaactc acaaagagca tcaaagaaaa acaaaataac gagttagaga
      361 aggtggccct taaacggaag gctgacagcg aagagaagct ggtgggaaag aaagaggcca
      421 agataatgga actagacaat cagctagtca ccaccgtacc tctgccccac atccccttaa
      481 agaacataat ggatgtggaa atgaagctgg tctatgttga tgaagaggat gtgagctatg
      541 agtttgccca gcctaacatg tgccttgggc ttcaggcaac aggccagact gccaccacga
      601 tgccacctgc aagtccccgg gatctcacca ccctgccaca gattgacaaa tggcttcagg
      661 tagccttaaa ggatgccagc tcttgctata gacagaagaa atatgccgtg gcagcaggac
      721 agttcaggac agcacttgag ctttgcagca aaggagcagc tctggggaaa ccctttgaag
      781 cacacgctga agacatagca agcatcgcaa gtttcattga gacaaagctc gtcacatgct
      841 acctacgtat gcggaagcct gaccttgccc tgaaccatgc gcacaggagt attgttttaa
      901 acccagccta tttccgaaac catcttcgtc aggcagccgt gtttagatgt ctggagagat
      961 actcagaagc tgcccggagt gccatgattg cggactacat gttctggctc tgtggaggga
     1021 gcgagcactc tgtaagcaag ctcatcaaac tgtattggca ggccatgatt gaagaagcca
     1081 tcaccagggc cgaagctttc tcggttatgt acacgccctt tgcaacaaga atcaagcctg
     1141 agaacataga aaaggtgaaa gaggtgttca tgagaactca ccccacctac gtggactgta
     1201 tctacacaga tacccaagga ctccatgtac tgccgcagac agccgactgg tcgtgttttc
     1261 ctccccagca atatctgctg acacttgggt tcaaaaacaa agaagacggg aaattcttgg
     1321 aaaaagtgtc cagtaggaag ctccccacct acacagaaca taaaactccc ttctctccac
     1381 tgaccagaga agatacagtg agacacatgg agacagtggg gaagagaatc ctgcccatcc
     1441 tggactttat ccggagcaca caactgaatg ggaatttcca tgcctgctca ggtgtgatgg
     1501 agaagttgca ctatgccagc ctcctcagcc ggctgcagag agtgaaggaa caggcccagg
     1561 tgatcaatca agcaatggca gagctggcaa cagtccctta tctccaggac atcagtcaac
     1621 aggaggcaga attgctgcag tcgctaatgg cagacgccat ggacaccctt gaaggaaaga
     1681 agagtgataa ggagcgtgtg tggaatacaa tccaaaaggt cggacgaata gaagacttcc
     1741 tgtaccaact agaagacagc ttcttaaaaa ctaagaaact cagaaccgct cgaaggcaga
     1801 agacaaagat gaagagactt caaactgtac agcaaaacta gacactagga agcaggctgc
     1861 ctgaagtgtc tggagaagtt ggggtctgta gcctctgggt aacaaaggct gtcacccaag
     1921 gcctcagatg gcaccctctt ggcaaagaca gtctacactt cattactttc aactggtagc
     1981 aataaatgtt ctcgacttgt tggacttatg c
//
