LOCUS       NM_009242               2641 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus secreted acidic cysteine rich glycoprotein (Sparc),
            transcript variant 1, mRNA.
ACCESSION   NM_009242 XM_911741
VERSION     NM_009242.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Li,C., Zhang,M., Du,Y., Liu,S., Li,D., Zhang,S., Ji,F., Zhang,J.
            and Jiao,J.
  TITLE     Compromised cell competition exhausts neural stem cells pool
  JOURNAL   Cell Prolif 57 (12), e13710 (2024)
   PUBMED   39010274
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Xiao,G., Wei,Y., Xie,R., Tsang,Y., Gu,J., Shen,D., Ding,M.,
            Yuan,J., Xu,D. and Fei,J.
  TITLE     Citric acid promotes SPARC release in pancreatic cancer cells and
            inhibits the progression of pancreatic tumors in mice on a high-fat
            diet
  JOURNAL   FEBS J 291 (8), 1699-1718 (2024)
   PUBMED   38245817
  REMARK    GeneRIF: Citric acid promotes SPARC release in pancreatic cancer
            cells and inhibits the progression of pancreatic tumors in mice on
            a high-fat diet.
REFERENCE   3  (bases 1 to 2641)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2641)
  AUTHORS   Arai,M., Kaku,M., Thant,L., Kitami,M., Ono,Y., Dobashi,A.,
            Iwama,H., Mizukoshi,M., Kitami,K., Matsumoto,M., Saito,I. and
            Uoshima,K.
  TITLE     Effect of Sparc knockout on the extracellular matrix of mouse
            periodontal ligament cells
  JOURNAL   Biochem Biophys Res Commun 692, 149364 (2024)
   PUBMED   38070276
  REMARK    GeneRIF: Effect of Sparc knockout on the extracellular matrix of
            mouse periodontal ligament cells.
REFERENCE   5  (bases 1 to 2641)
  AUTHORS   Queen,R., Crosier,M., Eley,L., Kerwin,J., Turner,J.E., Yu,J.,
            Alqahtani,A., Dhanaseelan,T., Overman,L., Soetjoadi,H., Baldock,R.,
            Coxhead,J., Boczonadi,V., Laude,A., Cockell,S.J., Kane,M.A.,
            Lisgo,S. and Henderson,D.J.
  TITLE     Spatial transcriptomics reveals novel genes during the remodelling
            of the embryonic human arterial valves
  JOURNAL   PLoS Genet 19 (11), e1010777 (2023)
   PUBMED   38011284
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2641)
  AUTHORS   Baron,B., Poirier,C., Simon-Chazottes,D., Barnier,C. and
            Guenet,J.L.
  TITLE     A new strategy useful for rapid identification of microsatellites
            from DNA libraries with large size inserts
  JOURNAL   Nucleic Acids Res 20 (14), 3665-3669 (1992)
   PUBMED   1386425
REFERENCE   7  (bases 1 to 2641)
  AUTHORS   Bloom,M.L., Lee,B.K., Birkenmeier,C.S., Ma,Y., Zimmer,W.E.,
            Goodman,S.R., Eicher,E.M. and Barker,J.E.
  TITLE     Brain beta spectrin isoform 235 (Spnb-2) maps to mouse chromosome
            11
  JOURNAL   Mamm Genome 3 (5), 293-295 (1992)
   PUBMED   1638088
REFERENCE   8  (bases 1 to 2641)
  AUTHORS   Le Roy,H., Simon-Chazottes,D., Montagutelli,X. and Guenet,J.L.
  TITLE     A set of anonymous DNA clones as markers for mouse gene mapping
  JOURNAL   Mamm Genome 3 (4), 244-246 (1992)
   PUBMED   1351769
REFERENCE   9  (bases 1 to 2641)
  AUTHORS   Baldacci,P.A., Richoux,V., Renard,J.P., Guenet,J.L. and Babinet,C.
  TITLE     The locus Om, responsible for the DDK syndrome, maps close to Sigje
            on mouse chromosome 11
  JOURNAL   Mamm Genome 2 (2), 100-105 (1992)
   PUBMED   1347470
REFERENCE   10 (bases 1 to 2641)
  AUTHORS   Buckwalter,M.S., Katz,R.W. and Camper,S.A.
  TITLE     Localization of the panhypopituitary dwarf mutation (df) on mouse
            chromosome 11 in an intersubspecific backcross
  JOURNAL   Genomics 10 (3), 515-526 (1991)
   PUBMED   1889803
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY781653.1, BB857564.1, AK161229.1, AA798908.1, CJ132527.1 and
            BE655213.1.
            
            On Mar 20, 2014 this sequence version replaced NM_009242.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.15933.1,
                                           SRR1660811.202887.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                BY781653.1         130-169
            41-213              BB857564.1         42-214
            214-1378            AK161229.1         2-1166
            1379-1379           AA798908.1         5-5
            1380-2286           AK161229.1         1167-2073
            2287-2533           CJ132527.1         317-563
            2534-2641           BE655213.1         230-337
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 33.04 cM"
     gene            1..2641
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /note="secreted acidic cysteine rich glycoprotein"
                     /db_xref="GeneID:20692"
                     /db_xref="MGI:MGI:98373"
     exon            1..293
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /inference="alignment:Splign:2.1.0"
     exon            294..363
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /inference="alignment:Splign:2.1.0"
     CDS             307..1215
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /note="basement-membrane protein 40; secreted protein
                     acidic and rich in cysteine; osteonectin"
                     /codon_start=1
                     /product="SPARC precursor"
                     /protein_id="NP_033268.1"
                     /db_xref="CCDS:CCDS24712.1"
                     /db_xref="GeneID:20692"
                     /db_xref="MGI:MGI:98373"
                     /translation="MRAWIFFLLCLAGRALAAPQQTEVAEEIVEEETVVEETGVPVGA
                     NPVQVEMGEFEDGAEETVEEVVADNPCQNHHCKHGKVCELDESNTPMCVCQDPTSCPA
                     PIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIAPCLDSELT
                     EFPLRMRDWLKNVLVTLYERDEGNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARD
                     FEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDN
                     DKYIALEEWAGCFGIKEQDINKDLVI"
     exon            364..423
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /inference="alignment:Splign:2.1.0"
     exon            424..511
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /inference="alignment:Splign:2.1.0"
     exon            512..633
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /inference="alignment:Splign:2.1.0"
     exon            634..754
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /inference="alignment:Splign:2.1.0"
     exon            755..888
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1037
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1186
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..2641
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2274..2279
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /note="hexamer: AATAAA"
     polyA_site      2300
                     /gene="Sparc"
                     /gene_synonym="BM-40; ON"
                     /note="major polyA site"
ORIGIN      
        1 aggtggggcc agggtgtctg ggtagcacac agcctaccac aaggcaagga agggagaaag
       61 aaaagacagc tttggttctg gggagaaggg ggaggcaggc caggggaagg aggagacaga
      121 ggaggaggag ggcctttgag tggaggcgga gggtgagaca gaccctgccc agagctccaa
      181 gaggcttcct gctgctcgcc tctaaacccc tccacattcc tgcagccctt cagaccgcca
      241 gaactcttct gccgcctgcc tgcctgcctg cctgcctgcc tgcctgtgcc gagagttccc
      301 agcatcatga gggcctggat cttctttctc ctttgcctgg ccgggagggc cctggcagcc
      361 cctcagcaga ctgaagttgc tgaggagata gtggaggagg aaaccgtggt ggaggagaca
      421 ggggtacctg tgggtgccaa cccagtccag gtggaaatgg gagaatttga ggacggtgca
      481 gaggaaacgg tcgaggaggt ggtggctgac aacccctgcc agaaccatca ttgcaaacat
      541 ggcaaggtgt gtgagctgga cgagagcaac acccccatgt gtgtgtgcca ggaccccacc
      601 agctgccctg ctcccattgg cgagtttgag aaggtatgca gcaatgacaa caagaccttc
      661 gactcttcct gccacttctt tgccaccaag tgcaccctgg agggcaccaa gaagggccac
      721 aagctccacc tggactacat cggaccatgc aaatacatcg ccccctgcct ggattccgag
      781 ctgactgaat tccctctgcg catgcgtgac tggctcaaaa atgtcctggt caccttgtac
      841 gagagagatg agggcaacaa cctcctcact gagaagcaga agctgcgtgt gaagaagatc
      901 catgagaatg agaagcgcct ggaggctgga gaccaccccg tggagctgtt ggcccgagac
      961 tttgagaaga actacaatat gtacatcttc cctgtccact ggcagtttgg ccagctggat
     1021 cagcacccta ttgatgggta cctgtcccac actgagctgg ccccactgcg cgctcccctc
     1081 atccccatgg aacattgcac cacacgtttc tttgagacct gtgacctaga caacgacaag
     1141 tacattgccc tggaggaatg ggccggctgc tttggcatca aggagcagga catcaacaag
     1201 gatctggtga tctaagttca cgcctcctgc tgcagtcctg aactctctcc ctctgatgtg
     1261 tcccccctcc cattaccccc ttgtttaaaa tgtttggatg gttggctgtt ccgcctgggg
     1321 ataaggtgct aacatagatt taactgaata cattaacggt gctaaaaaaa aaaaaaaaac
     1381 aaagtaagaa agaaactaga acccaagtca cagcattttc ccacataact ctgaggccat
     1441 ggcccatcca cagcctcctg gtcccctgca ctacccagtg tctcactggc tgtgttggaa
     1501 acggagttgc ataagctcac cgtccacaag cacgaggaga tatctctagc tttcatttct
     1561 gttttgcatt tgactcttaa cactcaccca gactctgtgc ttatttcatt ttgggggatg
     1621 tgggcttttt cccctggtgg tttggagtta ggcagaggga agttacagac acaggtacaa
     1681 aatttgggta aagatgctgt gagacctgag gacccaccag tcagaaccca catggcaagt
     1741 cttagtagcc taggtcaagg aaagacagaa taatccagag ctgtggcaca catgacagac
     1801 tcccagcagc ccgggacctt gctgtcttct cgactcttag ggcgtttctt tccatgtttg
     1861 gctgttggtt ttagttttgg tgagccatgg gtgggccaga acatcactca actgcaattg
     1921 ggctttcagg ttcttgccgg gagctctagg cactgggagg ctgtttcagg aaagtgagac
     1981 tcaagaggaa gacagaaaag gttgtaacgt agaggaagtg agactggtga attggtttga
     2041 tttttttcac atctagatgg ctgtcataaa gtttctagca tgttccccct cacctctccc
     2101 caccccctgc cacttgaaac cttctactaa tcaagagaaa cttccaagcc aacggaatgg
     2161 tcagatctca caggctgaga aattgttccc ctccaagcat ttcatgaaaa agctgcttct
     2221 cattaaccat gcaaactctc acagcaatgt gaagagcttg acaagtcttt caaaataaaa
     2281 agtaacaact tagaaacggc tgtcctgtcc cgggtgatgg tatgtgtgtc ttactctccg
     2341 gtgcttcgtt ttcaggtgtg cacaaacatg gccatacttg tgcacagaag caaacactcc
     2401 attgtgcttt tatacacaca cacatattca cacactcaga cagggaagat atggctcctg
     2461 tgagcactaa tttaacacat gaagaagcca gttcctgggc tgggatgtag ctcagttgat
     2521 aaagtgctta cccaatatga acaaagccca ggttcgatcg tcagcgcaac ctaagctggc
     2581 tgtggcagtg aatgtttgaa atcccagccc caaggaggta gaggcaggac agtcaaatgt
     2641 c
//
